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Protein

MAP kinase-activated protein kinase 2

Gene

MAPk-Ak2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei142 – 1421Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: FlyBase
  • cell surface receptor signaling pathway Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of cell size Source: FlyBase
  • protein autophosphorylation Source: GO_Central
  • protein phosphorylation Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • regulation of JNK cascade Source: FlyBase
  • response to salt stress Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-171007. p38MAPK events.
R-DME-199920. CREB phosphorylation.
R-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-3371453. Regulation of HSF1-mediated heat shock response.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-450302. activated TAK1 mediates p38 MAPK activation.
R-DME-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
SignaLinkiP49071.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-activated protein kinase 2 (EC:2.7.11.1)
Short name:
MAPK-activated protein kinase 2
Short name:
MAPKAP kinase 2
Short name:
MAPKAPK-2
Gene namesi
Name:MAPk-Ak2
ORF Names:CG3086
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0013987. MAPk-Ak2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359MAP kinase-activated protein kinase 2PRO_0000086291Add
BLAST

Post-translational modificationi

Phosphorylated and activated by MAP kinase.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49071.
PRIDEiP49071.

Expressioni

Gene expression databases

BgeeiP49071.
GenevisibleiP49071. DM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi69137. 3 interactions.
DIPiDIP-21714N.
IntActiP49071. 4 interactions.
MINTiMINT-760885.
STRINGi7227.FBpp0070802.

Structurei

3D structure databases

ProteinModelPortaliP49071.
SMRiP49071. Positions 9-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 281262Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0604. Eukaryota.
ENOG410XP8F. LUCA.
GeneTreeiENSGT00830000128274.
InParanoidiP49071.
KOiK04443.
OMAiRHIVNII.
OrthoDBiEOG786H3M.
PhylomeDBiP49071.

Family and domain databases

Gene3Di4.10.1170.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR027442. MAPKAPK_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLQNQRQP KTTPLTDDYV TSNTVLGYGI NGKVVQCTHR RTQQNYALKV
60 70 80 90 100
LLDSERARRE VDLHWRVSGC RYIVNIIDVY ENTFKDRKCL LVVMECMEGG
110 120 130 140 150
ELFQRIQDKA DGAFTEREAA QIMHEICAAV DYLHSRDIAH RDLKPENLLY
160 170 180 190 200
TTTQPNATLK LTDFGFAKET FTSYTLQTPC YTPYYVAPEV LGPEKYDKSC
210 220 230 240 250
DIWSLGVVMY IIMCGFPPFY SNHGLAISPG MKNRIRTGQY DFPDPEWTNV
260 270 280 290 300
SQAAKDLIKG MLNVDPSKRL RIQDVISNKW IAQYNAVPQT PLCTGRMLKE
310 320 330 340 350
AEETWPEVQE EMTRSLATMR VDYDQMQIKA LDKSNNPLLT KRRKKIEEME

LYMANATRN
Length:359
Mass (Da):41,401
Last modified:February 1, 1996 - v1
Checksum:i69C5F9A94D1511EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20757 mRNA. Translation: AAA86885.1.
AE014298 Genomic DNA. Translation: AAG22408.1.
PIRiJC4297.
RefSeqiNP_001188547.1. NM_001201618.1.
NP_001245536.1. NM_001258607.2.
NP_524769.1. NM_080030.5.
NP_788861.1. NM_176688.3.
UniGeneiDm.1442.

Genome annotation databases

EnsemblMetazoaiFBtr0070837; FBpp0070802; FBgn0013987.
FBtr0070839; FBpp0070804; FBgn0013987.
FBtr0302940; FBpp0292066; FBgn0013987.
FBtr0308577; FBpp0300801; FBgn0013987.
GeneIDi44573.
KEGGidme:Dmel_CG3086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20757 mRNA. Translation: AAA86885.1.
AE014298 Genomic DNA. Translation: AAG22408.1.
PIRiJC4297.
RefSeqiNP_001188547.1. NM_001201618.1.
NP_001245536.1. NM_001258607.2.
NP_524769.1. NM_080030.5.
NP_788861.1. NM_176688.3.
UniGeneiDm.1442.

3D structure databases

ProteinModelPortaliP49071.
SMRiP49071. Positions 9-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69137. 3 interactions.
DIPiDIP-21714N.
IntActiP49071. 4 interactions.
MINTiMINT-760885.
STRINGi7227.FBpp0070802.

Proteomic databases

PaxDbiP49071.
PRIDEiP49071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070837; FBpp0070802; FBgn0013987.
FBtr0070839; FBpp0070804; FBgn0013987.
FBtr0302940; FBpp0292066; FBgn0013987.
FBtr0308577; FBpp0300801; FBgn0013987.
GeneIDi44573.
KEGGidme:Dmel_CG3086.

Organism-specific databases

CTDi44573.
FlyBaseiFBgn0013987. MAPk-Ak2.

Phylogenomic databases

eggNOGiKOG0604. Eukaryota.
ENOG410XP8F. LUCA.
GeneTreeiENSGT00830000128274.
InParanoidiP49071.
KOiK04443.
OMAiRHIVNII.
OrthoDBiEOG786H3M.
PhylomeDBiP49071.

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-171007. p38MAPK events.
R-DME-199920. CREB phosphorylation.
R-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-3371453. Regulation of HSF1-mediated heat shock response.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-450302. activated TAK1 mediates p38 MAPK activation.
R-DME-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
SignaLinkiP49071.

Miscellaneous databases

GenomeRNAii44573.
PROiP49071.

Gene expression databases

BgeeiP49071.
GenevisibleiP49071. DM.

Family and domain databases

Gene3Di4.10.1170.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR027442. MAPKAPK_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Drosophila melanogaster homolog of the mammalian MAPK-activated protein kinase-2 (MAPKAPK-2) lacks a proline-rich N-terminus."
    Larochelle S., Suter B.
    Gene 163:209-214(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BIC-D(R26).
    Tissue: Ovary.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.

Entry informationi

Entry nameiMAPK2_DROME
AccessioniPrimary (citable) accession number: P49071
Secondary accession number(s): Q9W480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.