P49067 (AMYA_PYRFU) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 74.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-amylase EC=3.2.1.1 | ||||
| Gene names |
| ||||
| Organism | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) | ||||
| Taxonomic identifier | 186497 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus |
Protein attributes
| Sequence length | 649 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Displays a broad range of substrate specificity, with the capacity to hydrolyze carbohydrates as simple as maltotriose. |
| Catalytic activity | Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. |
| Subunit structure | Homodimer. |
| Sequence similarities | Belongs to the glycosyl hydrolase 57 family. |
| Biophysicochemical properties | pH dependence: Optimum pH is 6.5-7.5. Temperature dependence: Optimum temperature is 100 degrees Celsius. Inactive below 40 degrees Celsius. Extremely thermostable. |
| Sequence caution | The sequence AAL80396.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | alpha-amylase activity Inferred from electronic annotation. Source: EC alpha-mannosidase activityInferred from electronic annotation. Source: InterPro carbohydrate bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus. Cloning and sequencing of the gene and expression in Escherichia coli." Laderman K.A., Asada K., Uemori T., Mukai H., Taguchi Y., Kato I., Anfinsen C.B. J. Biol. Chem. 268:24402-24407(1993) [PubMed: 8226990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
| [2] | "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
| [3] | "The purification and characterization of an extremely thermostable alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus." Laderman K.A., Davis B.R., Krutzsch H.C., Lewis M.S., Griko Y.V., Privalov P.L., Anfinsen C.B. J. Biol. Chem. 268:24394-24401(1993) [PubMed: 8226989] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L22346 Unassigned DNA. Translation: AAA72035.1. AE009950 Genomic DNA. Translation: AAL80396.1. Different initiation. |
| PIR | A49512. |
| RefSeq | NP_578001.1. NC_003413.1. |
3D structure databases | |
| ProteinModelPortal | P49067. |
| SMR | P49067. Positions 3-646. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH57. Glycoside Hydrolase Family 57. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBPYRT00000005619; EBPYRP00000005480; EBPYRG00000005618. |
| GeneID | 1468106. |
| GenomeReviews | Gene locus PF0272 in contig AE009950_GR. |
| KEGG | pfu:PF0272. |
| NMPDR | fig|186497.1.peg.277. |
Phylogenomic databases | |
| GeneTree | EBGT00050000023113. |
| HOGENOM | HBG460637. |
| OMA | LTERVWE. |
| ProtClustDB | CLSK2459743. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-16304. |
Family and domain databases | |
| InterPro | IPR015179. A-amylase/a-glucTrfase_C. IPR015178. A-amylase/a-glucTrfase_central. IPR011013. Glyco_hydro-type_carb-bd. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR000602. Glyco_hydro_38_core. IPR004300. Glyco_hydro_57_N. [Graphical view] |
| Gene3D | G3DSA:3.20.110.10. Glyco_hydro_38_core. 1 hit. |
| KO | K07405. |
| Pfam | PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view] |
| ProDom | PD014405. DUF1925. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SUPFAM | SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | AMYA_PYRFU | ||||||||
| Accession | Primary (citable) accession number: P49067 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with