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Reviewed, UniProtKB/Swiss-Prot P49063 (PGLR2_ARATH)

Last modified November 3, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Exopolygalacturonase clone GBGA483
      Short name=ExoPG
    EC=3.2.1.67
Alternative name(s):
    Pectinase
    Galacturan 1,4-alpha-galacturonidase
Gene names
Ordered Locus Names: At3g07850
ORF Names: F17A17.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.

Catalytic activity

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

Subcellular location

Secreted. Secreted › cell wall.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 5 PbH1 repeats.

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Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiongalacturan 1,4-alpha-galacturonidase activity

Inferred from electronic annotation. Source: EC

polygalacturonase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 444421Exopolygalacturonase clone GBGA483
PRO_0000024800

Sites

Active site2611Proton donor By similarity
Active site2841 By similarity

Amino acid modifications

Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation3421N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict811S → G in CAA51033. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P49063-1 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: DF1D4C30FB1C3F11

FASTA44445,600
        10         20         30         40         50         60 
MVGSHKASGV LLVLLVVMAT TIANGTPVVD KAKNAATAVE DTAKNAATAV GGAAASVGAK 

        70         80         90        100        110        120 
VSGAKPGAAV DVKASGAKGD SKTDDSAAFA AAWKEACAAG STITVPKGEY MVESLEFKGP 

       130        140        150        160        170        180 
CKGPVTLELN GNFKAPATVK TTKPHAGWID FENIADFTLN GNKAIFDGQG SLAWKANDCA 

       190        200        210        220        230        240 
KTGKCNSLPI NIRFTGLTNS KINSITSTNS KLFHMNILNC KNITLSDIGI DAPPESLNTD 

       250        260        270        280        290        300 
GIHIGRSNGV NLIGAKIKTG DDCVSIGDGT ENLIVENVEC GPGHGISIGS LGRYPNEQPV 

       310        320        330        340        350        360 
KGVTVRKCLI KNTDNGVRIK TWPGSPPGIA SNILFEDITM DNVSLPVLID QEYCPYGHCK 

       370        380        390        400        410        420 
AGVPSQVKLS DVTIKGIKGT SATKVAVKLM CSKGVPCTNI ALSDINLVHN GKEGPAVSAC 

       430        440 
SNIKPILSGK LVPAACTEVA KPGP

« Hide

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References

« Hide 'large scale' references
[1]"Differential expression of a polygalacturonase gene family in Arabidopsis thaliana."
Torki M., Mandaron P., Thomas F., Quigley F., Mache R., Falconet D.
Mol. Gen. Genet. 261:948-952(1999) [PubMed: 10485285] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. C24.
Tissue: Flower bud.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X72292 mRNA. Translation: CAA51033.1.
AC013483 Genomic DNA. Translation: AAF21195.1.
AY056173 mRNA. Translation: AAL07022.1.
AY091200 mRNA. Translation: AAM14139.1.
IPIIPI00544320.
PIRS34200.
RefSeqNP_187442.1.
UniGeneAt.22234

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP49063.

Protein family/group databases

CAZyGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PRIDEP49063.
ProMEXP49063.

Genome annotation databases

GeneID819976.
GenomeReviewsGene locus AT3G07850 in contig BA000014_GR.
KEGGath:AT3G07850.
NMPDRfig|3702.1.peg.12823.

Organism-specific databases

GeneFarm575. 2.
TAIRAt3g07850.

Phylogenomic databases

OMAFENIADF.

Enzyme and pathway databases

BRENDA3.2.1.67. 302.

Gene expression databases

ArrayExpressP49063.
GenevestigatorP49063.
GermOnlineAT3G07850. Arabidopsis thaliana.

Family and domain databases

InterProIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 5 hits.
[Graphical view]
PROSITEPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePGLR2_ARATH
AccessionPrimary (citable) accession number: P49063
Secondary accession number(s): Q9SFC9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 10, 2003
Last modified: November 3, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents