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Protein

Exopolygalacturonase clone GBGE184

Gene

PGA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei241 – 2411Proton donorPROSITE-ProRule annotation
Active sitei264 – 2641PROSITE-ProRule annotation

GO - Molecular functioni

  • galacturan 1,4-alpha-galacturonidase activity Source: UniProtKB-EC
  • polygalacturonase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G02790-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolygalacturonase clone GBGE184 (EC:3.2.1.67)
Short name:
ExoPG
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Pectinase
Gene namesi
Name:PGA3
Ordered Locus Names:At1g02790
ORF Names:F22D16.22, T14P4_2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02790.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 422391Exopolygalacturonase clone GBGE184PRO_0000024799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence analysis
Glycosylationi252 – 2521N-linked (GlcNAc...)Sequence analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP49062.
PRIDEiP49062.

Expressioni

Gene expression databases

GenevisibleiP49062. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02790.1.

Structurei

3D structure databases

ProteinModelPortaliP49062.
SMRiP49062. Positions 53-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IH4B. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiP49062.
KOiK01213.
OMAiENIIMQS.
OrthoDBiEOG09360AHF.
PhylomeDBiP49062.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANARSLVAK ANNINVGSLI LMALVFGSCV ANGEYLGGRR GLAANSGNPT
60 70 80 90 100
VYDITKFGAV GDGSTNTFKA FLNTWIQVCD SPVPATLLVP KGTFLAGPVI
110 120 130 140 150
FAGPCKSKVT VNVIGTIIAT TSGYATPEWF LFERVDNLVL TGTGTFHGKG
160 170 180 190 200
EAVWKADGCG KKVQCNLPPT SLKFRNMKNV EINGISSVNA KAFHMFLVKT
210 220 230 240 250
ENVNIQNIKL TAPAESPNTD GIHLSNADNV SILDSTIATG DDCVSVGRGS
260 270 280 290 300
NNVTVERVIC GPGHGLSVGS LGKYKNEEDV SGIHVNNCTM IETDNGLRIK
310 320 330 340 350
TWGGSDPSKA VDIKFENIIM QSVKNPIIID QNYGSRGGDS QVAISDILFK
360 370 380 390 400
NIRGTTITKD VVQIMCSKSV PCQGVNVVDV NLDYVGKTGG EKKSSSGGLV
410 420
GALCDNANVI FGGKLSFPMC PK
Length:422
Mass (Da):44,430
Last modified:February 1, 1996 - v1
Checksum:i17B02E2CE979B46A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72291 mRNA. Translation: CAA51032.1.
Y16230 Genomic DNA. Translation: CAA76127.1.
AC009525 Genomic DNA. Translation: AAF02888.1.
CP002684 Genomic DNA. Translation: AEE27470.1.
AF428425 mRNA. Translation: AAL16194.1.
AY065210 mRNA. Translation: AAL38686.1.
AY133812 mRNA. Translation: AAM91746.1.
PIRiS34199.
RefSeqiNP_171778.1. NM_100158.2.
UniGeneiAt.22350.

Genome annotation databases

EnsemblPlantsiAT1G02790.1; AT1G02790.1; AT1G02790.
GeneIDi839391.
GrameneiAT1G02790.1; AT1G02790.1; AT1G02790.
KEGGiath:AT1G02790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72291 mRNA. Translation: CAA51032.1.
Y16230 Genomic DNA. Translation: CAA76127.1.
AC009525 Genomic DNA. Translation: AAF02888.1.
CP002684 Genomic DNA. Translation: AEE27470.1.
AF428425 mRNA. Translation: AAL16194.1.
AY065210 mRNA. Translation: AAL38686.1.
AY133812 mRNA. Translation: AAM91746.1.
PIRiS34199.
RefSeqiNP_171778.1. NM_100158.2.
UniGeneiAt.22350.

3D structure databases

ProteinModelPortaliP49062.
SMRiP49062. Positions 53-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G02790.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiP49062.
PRIDEiP49062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02790.1; AT1G02790.1; AT1G02790.
GeneIDi839391.
GrameneiAT1G02790.1; AT1G02790.1; AT1G02790.
KEGGiath:AT1G02790.

Organism-specific databases

TAIRiAT1G02790.

Phylogenomic databases

eggNOGiENOG410IH4B. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiP49062.
KOiK01213.
OMAiENIIMQS.
OrthoDBiEOG09360AHF.
PhylomeDBiP49062.

Enzyme and pathway databases

BioCyciARA:AT1G02790-MONOMER.

Miscellaneous databases

PROiP49062.

Gene expression databases

GenevisibleiP49062. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR1_ARATH
AccessioniPrimary (citable) accession number: P49062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.