P49062 (PGLR1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exopolygalacturonase clone GBGE184
Short name=ExoPG
EC=3.2.1.67

Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Pectinase

Gene names

Name:	PGA3
Ordered Locus Names:	At1g02790
ORF Names:	F22D16.22, T14P4_2

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.
Catalytic activity	((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.
Subcellular location	Secreted. Secreted › cell wall.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galacturan 1,4-alpha-galacturonidase activity Inferred from electronic annotation. Source: EC polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 422	391	Exopolygalacturonase clone GBGE184		PRO_0000024799
Sites
	Active site	241	1	Proton donor By similarity
	Active site	264	1	By similarity
Amino acid modifications
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49062 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 17B02E2CE979B46A	FASTA	422	44,430
	10 20 30 40 50 60 MANARSLVAK ANNINVGSLI LMALVFGSCV ANGEYLGGRR GLAANSGNPT VYDITKFGAV 70 80 90 100 110 120 GDGSTNTFKA FLNTWIQVCD SPVPATLLVP KGTFLAGPVI FAGPCKSKVT VNVIGTIIAT 130 140 150 160 170 180 TSGYATPEWF LFERVDNLVL TGTGTFHGKG EAVWKADGCG KKVQCNLPPT SLKFRNMKNV 190 200 210 220 230 240 EINGISSVNA KAFHMFLVKT ENVNIQNIKL TAPAESPNTD GIHLSNADNV SILDSTIATG 250 260 270 280 290 300 DDCVSVGRGS NNVTVERVIC GPGHGLSVGS LGKYKNEEDV SGIHVNNCTM IETDNGLRIK 310 320 330 340 350 360 TWGGSDPSKA VDIKFENIIM QSVKNPIIID QNYGSRGGDS QVAISDILFK NIRGTTITKD 370 380 390 400 410 420 VVQIMCSKSV PCQGVNVVDV NLDYVGKTGG EKKSSSGGLV GALCDNANVI FGGKLSFPMC PK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of a polygalacturonase gene family in Arabidopsis thaliana." Torki M., Mandaron P., Thomas F., Quigley F., Mache R., Falconet D. Mol. Gen. Genet. 261:948-952(1999) [PubMed: 10485285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C24. Tissue: Flower bud.
[2]	"Organisation and expression of a family of polygalacturonase gene in Arabidopsis thaliana." Torki M., Thomas F., Mache R., Mandaron P., Falconet D. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72291 mRNA. Translation: CAA51032.1. Y16230 Genomic DNA. Translation: CAA76127.1. AC009525 Genomic DNA. Translation: AAF02888.1. CP002684 Genomic DNA. Translation: AEE27470.1. AF428425 mRNA. Translation: AAL16194.1. AY065210 mRNA. Translation: AAL38686.1. AY133812 mRNA. Translation: AAM91746.1.
IPI	IPI00532768.
PIR	S34199.
RefSeq	NP_171778.1. NM_100158.2.
UniGene	At.22350.
3D structure databases
ProteinModelPortal	P49062.
SMR	P49062. Positions 47-101.
ModBase	Search...
Protein-protein interaction databases
STRING	P49062.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	P49062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02790.1; AT1G02790.1; AT1G02790.
GeneID	839391.
GenomeReviews	Gene locus AT1G02790 in contig CT485782_GR.
KEGG	ath:AT1G02790.
NMPDR	fig|3702.1.peg.400.
Organism-specific databases
TAIR	At1g02790.
Phylogenomic databases
eggNOG	COG5434.
HOGENOM	HBG744658.
InParanoid	P49062.
OMA	IIDQNYG.
PhylomeDB	P49062.
ProtClustDB	CLSN2912732.
Gene expression databases
ArrayExpress	P49062.
Genevestigator	P49062.
GermOnline	AT1G02790. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01213.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_ARATH

Accession

Primary (citable) accession number: P49062

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program