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Protein

Exopolygalacturonase clone GBGE184

Gene

PGA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei241Proton donorBy similarity1
Active sitei264PROSITE-ProRule annotation1

GO - Molecular functioni

  • galacturan 1,4-alpha-galacturonidase activity Source: UniProtKB-EC
  • polygalacturonase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT1G02790-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolygalacturonase clone GBGE184 (EC:3.2.1.67)
Short name:
ExoPG
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Pectinase
Gene namesi
Name:PGA3
Ordered Locus Names:At1g02790
ORF Names:F22D16.22, T14P4_2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G02790.
TAIRilocus:2024680. AT1G02790.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000002479932 – 422Exopolygalacturonase clone GBGE184Add BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi229N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi243 ↔ 260By similarity
Glycosylationi252N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi287N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi366 ↔ 372By similarity
Disulfide bondi404 ↔ 420By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49062.

Expressioni

Gene expression databases

ExpressionAtlasiP49062. baseline and differential.
GenevisibleiP49062. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02790.1.

Structurei

3D structure databases

ProteinModelPortaliP49062.
SMRiP49062.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati200 – 226PbH1 1Sequence analysisAdd BLAST27
Repeati227 – 248PbH1 2Sequence analysisAdd BLAST22
Repeati250 – 270PbH1 3Sequence analysisAdd BLAST21
Repeati280 – 301PbH1 4Sequence analysisAdd BLAST22
Repeati310 – 331PbH1 5Sequence analysisAdd BLAST22

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IH4B. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiP49062.
KOiK01213.
OMAiHEWINFA.
OrthoDBiEOG09360AHF.
PhylomeDBiP49062.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF00295. Glyco_hydro_28. 1 hit.
SMARTiView protein in SMART
SM00710. PbH1. 5 hits.
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiView protein in PROSITE
PS00502. POLYGALACTURONASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANARSLVAK ANNINVGSLI LMALVFGSCV ANGEYLGGRR GLAANSGNPT
60 70 80 90 100
VYDITKFGAV GDGSTNTFKA FLNTWIQVCD SPVPATLLVP KGTFLAGPVI
110 120 130 140 150
FAGPCKSKVT VNVIGTIIAT TSGYATPEWF LFERVDNLVL TGTGTFHGKG
160 170 180 190 200
EAVWKADGCG KKVQCNLPPT SLKFRNMKNV EINGISSVNA KAFHMFLVKT
210 220 230 240 250
ENVNIQNIKL TAPAESPNTD GIHLSNADNV SILDSTIATG DDCVSVGRGS
260 270 280 290 300
NNVTVERVIC GPGHGLSVGS LGKYKNEEDV SGIHVNNCTM IETDNGLRIK
310 320 330 340 350
TWGGSDPSKA VDIKFENIIM QSVKNPIIID QNYGSRGGDS QVAISDILFK
360 370 380 390 400
NIRGTTITKD VVQIMCSKSV PCQGVNVVDV NLDYVGKTGG EKKSSSGGLV
410 420
GALCDNANVI FGGKLSFPMC PK
Length:422
Mass (Da):44,430
Last modified:February 1, 1996 - v1
Checksum:i17B02E2CE979B46A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72291 mRNA. Translation: CAA51032.1.
Y16230 Genomic DNA. Translation: CAA76127.1.
AC009525 Genomic DNA. Translation: AAF02888.1.
CP002684 Genomic DNA. Translation: AEE27470.1.
AF428425 mRNA. Translation: AAL16194.1.
AY065210 mRNA. Translation: AAL38686.1.
AY133812 mRNA. Translation: AAM91746.1.
PIRiS34199.
RefSeqiNP_171778.1. NM_100158.3.
UniGeneiAt.22350.

Genome annotation databases

EnsemblPlantsiAT1G02790.1; AT1G02790.1; AT1G02790.
GeneIDi839391.
GrameneiAT1G02790.1; AT1G02790.1; AT1G02790.
KEGGiath:AT1G02790.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPGLR1_ARATH
AccessioniPrimary (citable) accession number: P49062
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 7, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families