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Protein

Probable sucrose-phosphate synthase

Gene

SPS

Organism
Beta vulgaris (Sugar beet)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in photosynthetic sucrose synthesis by catalyzing the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose- 6-phosphate. Involved in the regulation of carbon partitioning in the leaves of plants. May regulate the synthesis of sucrose and therefore play a major role as a limiting factor in the export of photoassimilates out of the leaf. Plays a role for sucrose availability that is essential for plant growth and fiber elongation (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate.

Enzyme regulationi

Activity is regulated by phosphorylation and moderated by concentration of metabolites and light.By similarity

Pathwayi: sucrose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable sucrose-phosphate synthase (SPS)
  2. no protein annotated in this organism
This subpathway is part of the pathway sucrose biosynthesis, which is itself part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose, the pathway sucrose biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable sucrose-phosphate synthase (EC:2.4.1.14)
Alternative name(s):
UDP-glucose-fructose-phosphate glucosyltransferase
Gene namesi
Name:SPS
OrganismiBeta vulgaris (Sugar beet)
Taxonomic identifieri161934 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeBetoideaeBeta

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10451045Probable sucrose-phosphate synthasePRO_0000204667Add
BLAST

Expressioni

Tissue specificityi

Predominantly active in tap root.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP49031.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

KOiK00696.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.

Sequencei

Sequence statusi: Complete.

P49031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNDWINSY LEAILDVGPG LDDAKSSLLL RERGRFSPTR YFVEEVITGF
60 70 80 90 100
DETDLHRSWV RAQATRSPQE RNTRLENMCW RIWNLARQKK QLENEEAQRK
110 120 130 140 150
TKRRMELERG RREATADMSE DLSEGEKDIS AHGDSTRPRL PRINSLDAME
160 170 180 190 200
TWISQQKEKK LYLVLISLHG LIRGENMELG RDSDTGGQVK YVVELARALG
210 220 230 240 250
SMPGVYRVDL LTRQVSSPDV DWSYGEPTEM LNPRDSNGFD DDDDEMGESS
260 270 280 290 300
GAYIVRIPFG PRDKYIAKEE LWPYIPEFVD GALNHIVQMS KVLGEQIGSG
310 320 330 340 350
ETVWPVAIHG HYADAGDSAA LLSGGLNVPM LLTGHSLGRD KLEQLLKQGR
360 370 380 390 400
MSKDDINNTY KIMRRIEAEE LSLDASEIVI TSTRQEIEEQ WHLYDGFDPV
410 420 430 440 450
LERKLRARMK RGVSCYGRFM PRMVVIPPGM EFNHIVPHEG DMDGETEETE
460 470 480 490 500
EHPTSPDPPI WAEIMRFFSK PRKPMILALA RPDPKKNITT LVKAFGECRP
510 520 530 540 550
LRELANLTLI MGNRDGIDEM SSTSSSVLLS VLKLIDQYDL YGQVAYPKHH
560 570 580 590 600
KQADVPEIYR LAAKTKGVFI NPAFIEPFGL TLIEAAAHGL PMVATKNGGP
610 620 630 640 650
VDIQRVLDNG LLVDPHEQQS IATALLKLVA DKQLWTKCQQ NGLKNIHLYS
660 670 680 690 700
WPEHSKTYLS RIASSRQRQP QWQRSSDEGL DNQEPESPSD SLRDIKDISL
710 720 730 740 750
NLEVLVRPEK RVKTLKILGL MTKANSRMLL CSWSNGVHKM LRKARFSDKV
760 770 780 790 800
DQASSKYPAF RRRKLIYVIA VDGDYEDGLF DIVRRIFDAA GKEKIEGSIG
810 820 830 840 850
FILSTSYSMP EIQNYLLSKG FNLHDFDAYI CNSGSELYYS SLNSEESNII
860 870 880 890 900
ADSDYHSHIE YRWGGEGLRR TLLRWAASIT EKNGENEEQV ITEDEEVSTG
910 920 930 940 950
YCFAFKIKNQ NKVPPTKELR KSMRIQALRC HVIYCQNGSK MNVIPVLASR
960 970 980 990 1000
SQALRYLYVR WGVELSKMVV FVGECGDTDY EGLLGGVHKT VILKGVSNTA
1010 1020 1030 1040
LRSLHANRSY PLSHVVSLDS PNIGEVSKGC SSSEIQSIVT KLSKA
Length:1,045
Mass (Da):118,181
Last modified:February 1, 1996 - v1
Checksum:i8AEC555A5F805307
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81975 mRNA. Translation: CAA57500.1.
PIRiS55253.
RefSeqiNP_001289997.1. NM_001303068.1.

Genome annotation databases

GeneIDi104901950.
KEGGibvg:104901950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81975 mRNA. Translation: CAA57500.1.
PIRiS55253.
RefSeqiNP_001289997.1. NM_001303068.1.

3D structure databases

ProteinModelPortaliP49031.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi104901950.
KEGGibvg:104901950.

Phylogenomic databases

KOiK00696.

Enzyme and pathway databases

UniPathwayiUPA00371; UER00545.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR006380. SPP_N.
IPR000368. Sucrose_synth.
IPR012819. SucrsPsyn_pln.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF05116. S6PP. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02468. sucrsPsyn_pln. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression analysis of sucrose-phosphate synthase from sugar beet (Beta vulgaris L.)."
    Hesse H., Sonnewald U., Willmitzer L.
    Mol. Gen. Genet. 247:515-520(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Tap root.

Entry informationi

Entry nameiSPSA_BETVU
AccessioniPrimary (citable) accession number: P49031
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 13, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.