P49025 (CTRO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citron Rho-interacting kinase
Short name=CRIK
EC=2.7.11.1

Alternative name(s):
Rho-interacting, serine/threonine-protein kinase 21

Gene names

Name:	Cit
Synonyms:	Crik

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2055 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2 By similarity. Ref.1 Ref.2 Ref.5 Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.2
Subunit structure	Interacts with TTC3 By similarity. Homodimer Probable. Directly interacts with KIF14 depending on the activation state (stronger interaction with the kinase-dead form).
Subcellular location	Cytoplasm Ref.2.
Tissue specificity	A major signal was observed in testis and brain, but it was also detected in thymus, spleen, kidney, heart and lung. Ref.2 Ref.7
Developmental stage	Detected at E10.5 with highest expression in the developing central nervous system. After E16.5 expression decreases and at two weeks after birth is restricted to the proliferating neuronal precursor cells in the external germinal layer of the cerebellum and subventricular migratory stream. Ref.7
Disruption phenotype	Death before reaching adulthood, probably due to lethal epilepsy. Mice display severe defects in the olfactory bulbs, the hippocampus, and the cerebellum. These defects appear to result from impaired cytokinesis followed by the induction of apoptosis in specific neuroblast populations. Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
Sequence caution	The sequence AAC72822.1 differs from that shown. Reason: Frameshift at positions 396 and 408. The sequence AAC72823.1 differs from that shown. Reason: Frameshift at positions 396 and 408. The sequence AAH23775.1 differs from that shown. Reason: Frameshift at position 1011. The sequence AAH51165.1 differs from that shown. Reason: Frameshift at position 1011.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil SH3-binding Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell cycle cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB generation of neurons Inferred from direct assay Ref.7. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro mitosis Inferred from direct assay Ref.7. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.2. Source: UniProtKB small GTPase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P49025-1)

Also known as: CRIK;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49025-2)

Also known as: CRIK-SK;

The sequence of this isoform differs from the canonical sequence as follows:
     467-494: MEQEMTRLHRRVSEVEAVLSQKEVELKA → VSISTAGLRPCSRILQSIYAEGSAGGHC
     495-2055: Missing.

Isoform 3 (identifier: P49025-3)

Also known as: Citron;

The sequence of this isoform differs from the canonical sequence as follows:
     1-458: Missing.
     459-466: DSQDKCHK → MLLGEEAM

Isoform 4 (identifier: P49025-4)

The sequence of this isoform differs from the canonical sequence as follows:
     693-735: Missing.

Isoform 5 (identifier: P49025-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1279-1279: K → KGLFSRRKEDPALPTQ
     1602-1602: A → AARDHTSSEHQPVWVE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2055	2055	Citron Rho-interacting kinase		PRO_0000085909
Regions
	Domain	97 – 359	263	Protein kinase
	Domain	360 – 430	71	AGC-kinase C-terminal
	Domain	1469 – 1589	121	PH
	Domain	1617 – 1907	291	CNH
	Nucleotide binding	103 – 111	9	ATP By similarity
	Zinc finger	1388 – 1437	50	Phorbol-ester/DAG-type
	Region	1132 – 1328	197	Interaction with Rho/Rac
	Coiled coil	441 – 1086	646	Potential
	Coiled coil	1091 – 1247	157	Potential
	Coiled coil	1275 – 1325	51	Potential
	Motif	1979 – 1984	6	SH3-binding Potential
	Compositional bias	550 – 962	413	Glu-rich
	Compositional bias	1276 – 1279	4	Poly-Lys
Sites
	Active site	221	1	Proton acceptor By similarity
	Binding site	126	1	ATP By similarity
Amino acid modifications
	Modified residue	432	1	Phosphoserine By similarity
	Modified residue	439	1	Phosphoserine By similarity
	Modified residue	479	1	Phosphoserine Ref.8
	Modified residue	1237	1	Phosphotyrosine Ref.9
	Modified residue	1504	1	Phosphotyrosine Ref.9
	Modified residue	1608	1	Phosphoserine Ref.10
	Modified residue	1747	1	N6-acetyllysine By similarity
	Modified residue	1999	1	Phosphoserine By similarity
	Modified residue	2021	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 458	458	Missing in isoform 3.		VSP_012436
	Alternative sequence	459 – 466	8	DSQDKCHK → MLLGEEAM in isoform 3.		VSP_012437
	Alternative sequence	467 – 494	28	MEQEM…VELKA → VSISTAGLRPCSRILQSIYA EGSAGGHC in isoform 2.		VSP_012438
	Alternative sequence	495 – 2055	1561	Missing in isoform 2.		VSP_012439
	Alternative sequence	693 – 735	43	Missing in isoform 4.		VSP_016093
	Alternative sequence	1279	1	K → KGLFSRRKEDPALPTQ in isoform 5.		VSP_016094
	Alternative sequence	1602	1	A → AARDHTSSEHQPVWVE in isoform 5.		VSP_016095
Experimental info
	Mutagenesis	126	1	K → A: Loss of phosphorylation. Ref.2
	Sequence conflict	78	1	Q → R in BAE26199. Ref.3
	Sequence conflict	182	1	F → L in BAE26199. Ref.3
	Sequence conflict	234	1	E → H in BAE26199. Ref.3
	Sequence conflict	1945	1	Missing in AAH51165. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CRIK) [UniParc]. Last modified November 8, 2005. Version 3. Checksum: BDA9A12C14E12DF8	FASTA	2,055	235,389
	10 20 30 40 50 60 MLKFKYGVRN PPEASASEPI ASRASRLNLF FQGKPPLMTQ QQMSALSREG MLDALFALFE 70 80 90 100 110 120 ECSQPALMKM KHVSSFVQKY SDTIAELREL QPSARDFEVR SLVGCGHFAE VQVVREKATG 130 140 150 160 170 180 DVYAMKIMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNNL YLVMEYQPGG 190 200 210 220 230 240 DFLSLLNRYE DQLDESMIQF YLAELILAVH SVHQMGYVHR DIKPENILID RTGEIKLVDF 250 260 270 280 290 300 GSAAKMNSNK VDAKLPIGTP DYMAPEVLTV MNEDRRGTYG LDCDWWSVGV VAYEMVYGKT 310 320 330 340 350 360 PFTEGTSART FNNIMNFQRF LKFPDDPKVS SELLDLLQSL LCVQKERLKF EGLCCHPFFA 370 380 390 400 410 420 RTDWNNIRNS PPPFVPTLKS DDDTSNFDEP EKNSWVSSSV CQLSPSGFSG EELPFVGFSY 430 440 450 460 470 480 SKALGYLGRS ESVVSSLDSP AKVSSMEKKL LIKSKELQDS QDKCHKMEQE MTRLHRRVSE 490 500 510 520 530 540 VEAVLSQKEV ELKASETQRS LLEQDLATYI TECSSLKRSL EQARMEVSQE DDKALQLLHD 550 560 570 580 590 600 IREQSRKLQE IKEQEYQAQV EEMRLMMNQL EEDLVSARRR SDLYESELRE SRLAAEEFKR 610 620 630 640 650 660 KANECQHKLM KAKDQGKPEV GEYSKLEKIN AEQQLKIQEL QEKLEKAVKA STEATELLQN 670 680 690 700 710 720 IRQAKERAER ELEKLHNRED SSEGIKKKLV EAEERRHSLE NKVKRLETME RRENRLKDDI 730 740 750 760 770 780 QTKSEQIQQM ADKILELEEK HREAQVSAQH LEVHLKQKEQ HYEEKIKVLD NQIKKDLADK 790 800 810 820 830 840 ESLENMMQRH EEEAHEKGKI LSEQKAMINA MDSKIRSLEQ RIVELSEANK LAANSSLFTQ 850 860 870 880 890 900 RNMKAQEEMI SELRQQKFYL ETQAGKLEAQ NRKLEEQLEK ISHQDHSDKS RLLELETRLR 910 920 930 940 950 960 EVSLEHEEQK LELKRQLTEL QLSLQERESQ LTALQAARAA LESQLRQAKT ELEETTAEAE 970 980 990 1000 1010 1020 EEIQALTAHR DEIQRKFDAL RNSCTVITDL EEQLNQLTED NAELNNQNFY LSKQLDEASG 1030 1040 1050 1060 1070 1080 ANDEIVQLRS EVDHLRREIT EREMQLTSQK QTMEALKTTC TMLEEQVLDL EALNDELLEK 1090 1100 1110 1120 1130 1140 ERQWEAWRSV LGDEKSQFEC RVRELQRMLD TEKQSRARAD QRITESRQVV ELAVKEHKAE 1150 1160 1170 1180 1190 1200 ILALQQALKE QKLKAESLSD KLNDLEKKHA MLEMNARSLQ QKLETERELK QRLLEEQAKL 1210 1220 1230 1240 1250 1260 QQQMDLQKNH IFRLTQGLQE ALDRADLLKT ERSDLEYQLE NIQVLYSHEK VKMEGTISQQ 1270 1280 1290 1300 1310 1320 TKLIDFLQAK MDQPAKKKKV PLQYNELKLA LEKEKARCAE LEEALQKTRI ELRSAREEAA 1330 1340 1350 1360 1370 1380 HRKATDHPHP STPATARQQI AMSAIVRSPE HQPSAMSLLA PPSSRRKESS TPEEFSRRLK 1390 1400 1410 1420 1430 1440 ERMHHNIPHR FNVGLNMRAT KCAVCLDTVH FGRQASKCLE CQVMCHPKCS TCLPATCGLP 1450 1460 1470 1480 1490 1500 AEYATHFTEA FCRDKMNSPG LQSKEPGSSL HLEGWMKVPR NNKRGQQGWD RKYIVLEGSK 1510 1520 1530 1540 1550 1560 VLIYDNEARE AGQRPVEEFE LCLPDGDVSI HGAVGASELA NTAKADVPYI LKMESHPHTT 1570 1580 1590 1600 1610 1620 CWPGRTLYLL APSFPDKQRW VTALESVVAG GRVSREKAEA DAKLLGNSLL KLEGDDRLDM 1630 1640 1650 1660 1670 1680 NCTLPFSDQV VLVGTEEGLY ALNVLKNSLT HIPGIGAVFQ IYIIKDLEKL LMIAGEERAL 1690 1700 1710 1720 1730 1740 CLVDVKKVKQ SLAQSHLPAQ PDVSPNIFEA VKGCHLFAAG KIENSLCICA AMPSKVVILR 1750 1760 1770 1780 1790 1800 YNDNLSKYCI RKEIETSEPC SCIHFTNYSI LIGTNKFYEI DMKQYTLDEF LDKNDHSLAP 1810 1820 1830 1840 1850 1860 AVFASSSNSF PVSIVQANSA GQREEYLLCF HEFGVFVDSY GRRSRTDDLK WSRLPLAFAY 1870 1880 1890 1900 1910 1920 REPYLFVTHF NSLEVIEIQA RSSLGSPARA YLEIPNPRYL GPAISSGAIY LASSYQDKLR 1930 1940 1950 1960 1970 1980 VICCKGNLVK ESGTEQHRVP STSRSSPNKR GPPTYNEHIT KRVASSPAPP EGPSHPREPS 1990 2000 2010 2020 2030 2040 TPHRYRDREG RTELRRDKSP GRPLEREKSP GRMLSTRRER SPGRLFEDSS RGRLPAGAVR 2050 TPLSQVNKVW DQSSV
	Isoform 2 (CRIK-SK) [UniParc]. Checksum: E4AEAEA4C22D0051	FASTA	494	55,650
	10 20 30 40 50 60 MLKFKYGVRN PPEASASEPI ASRASRLNLF FQGKPPLMTQ QQMSALSREG MLDALFALFE 70 80 90 100 110 120 ECSQPALMKM KHVSSFVQKY SDTIAELREL QPSARDFEVR SLVGCGHFAE VQVVREKATG 130 140 150 160 170 180 DVYAMKIMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNNL YLVMEYQPGG 190 200 210 220 230 240 DFLSLLNRYE DQLDESMIQF YLAELILAVH SVHQMGYVHR DIKPENILID RTGEIKLVDF 250 260 270 280 290 300 GSAAKMNSNK VDAKLPIGTP DYMAPEVLTV MNEDRRGTYG LDCDWWSVGV VAYEMVYGKT 310 320 330 340 350 360 PFTEGTSART FNNIMNFQRF LKFPDDPKVS SELLDLLQSL LCVQKERLKF EGLCCHPFFA 370 380 390 400 410 420 RTDWNNIRNS PPPFVPTLKS DDDTSNFDEP EKNSWVSSSV CQLSPSGFSG EELPFVGFSY 430 440 450 460 470 480 SKALGYLGRS ESVVSSLDSP AKVSSMEKKL LIKSKELQDS QDKCHKVSIS TAGLRPCSRI 490 LQSIYAEGSA GGHC
	Isoform 3 (Citron) [UniParc]. Checksum: 7B7286C2305676DA	FASTA	1,597	183,449
	10 20 30 40 50 60 MLLGEEAMME QEMTRLHRRV SEVEAVLSQK EVELKASETQ RSLLEQDLAT YITECSSLKR 70 80 90 100 110 120 SLEQARMEVS QEDDKALQLL HDIREQSRKL QEIKEQEYQA QVEEMRLMMN QLEEDLVSAR 130 140 150 160 170 180 RRSDLYESEL RESRLAAEEF KRKANECQHK LMKAKDQGKP EVGEYSKLEK INAEQQLKIQ 190 200 210 220 230 240 ELQEKLEKAV KASTEATELL QNIRQAKERA ERELEKLHNR EDSSEGIKKK LVEAEERRHS 250 260 270 280 290 300 LENKVKRLET MERRENRLKD DIQTKSEQIQ QMADKILELE EKHREAQVSA QHLEVHLKQK 310 320 330 340 350 360 EQHYEEKIKV LDNQIKKDLA DKESLENMMQ RHEEEAHEKG KILSEQKAMI NAMDSKIRSL 370 380 390 400 410 420 EQRIVELSEA NKLAANSSLF TQRNMKAQEE MISELRQQKF YLETQAGKLE AQNRKLEEQL 430 440 450 460 470 480 EKISHQDHSD KSRLLELETR LREVSLEHEE QKLELKRQLT ELQLSLQERE SQLTALQAAR 490 500 510 520 530 540 AALESQLRQA KTELEETTAE AEEEIQALTA HRDEIQRKFD ALRNSCTVIT DLEEQLNQLT 550 560 570 580 590 600 EDNAELNNQN FYLSKQLDEA SGANDEIVQL RSEVDHLRRE ITEREMQLTS QKQTMEALKT 610 620 630 640 650 660 TCTMLEEQVL DLEALNDELL EKERQWEAWR SVLGDEKSQF ECRVRELQRM LDTEKQSRAR 670 680 690 700 710 720 ADQRITESRQ VVELAVKEHK AEILALQQAL KEQKLKAESL SDKLNDLEKK HAMLEMNARS 730 740 750 760 770 780 LQQKLETERE LKQRLLEEQA KLQQQMDLQK NHIFRLTQGL QEALDRADLL KTERSDLEYQ 790 800 810 820 830 840 LENIQVLYSH EKVKMEGTIS QQTKLIDFLQ AKMDQPAKKK KVPLQYNELK LALEKEKARC 850 860 870 880 890 900 AELEEALQKT RIELRSAREE AAHRKATDHP HPSTPATARQ QIAMSAIVRS PEHQPSAMSL 910 920 930 940 950 960 LAPPSSRRKE SSTPEEFSRR LKERMHHNIP HRFNVGLNMR ATKCAVCLDT VHFGRQASKC 970 980 990 1000 1010 1020 LECQVMCHPK CSTCLPATCG LPAEYATHFT EAFCRDKMNS PGLQSKEPGS SLHLEGWMKV 1030 1040 1050 1060 1070 1080 PRNNKRGQQG WDRKYIVLEG SKVLIYDNEA REAGQRPVEE FELCLPDGDV SIHGAVGASE 1090 1100 1110 1120 1130 1140 LANTAKADVP YILKMESHPH TTCWPGRTLY LLAPSFPDKQ RWVTALESVV AGGRVSREKA 1150 1160 1170 1180 1190 1200 EADAKLLGNS LLKLEGDDRL DMNCTLPFSD QVVLVGTEEG LYALNVLKNS LTHIPGIGAV 1210 1220 1230 1240 1250 1260 FQIYIIKDLE KLLMIAGEER ALCLVDVKKV KQSLAQSHLP AQPDVSPNIF EAVKGCHLFA 1270 1280 1290 1300 1310 1320 AGKIENSLCI CAAMPSKVVI LRYNDNLSKY CIRKEIETSE PCSCIHFTNY SILIGTNKFY 1330 1340 1350 1360 1370 1380 EIDMKQYTLD EFLDKNDHSL APAVFASSSN SFPVSIVQAN SAGQREEYLL CFHEFGVFVD 1390 1400 1410 1420 1430 1440 SYGRRSRTDD LKWSRLPLAF AYREPYLFVT HFNSLEVIEI QARSSLGSPA RAYLEIPNPR 1450 1460 1470 1480 1490 1500 YLGPAISSGA IYLASSYQDK LRVICCKGNL VKESGTEQHR VPSTSRSSPN KRGPPTYNEH 1510 1520 1530 1540 1550 1560 ITKRVASSPA PPEGPSHPRE PSTPHRYRDR EGRTELRRDK SPGRPLEREK SPGRMLSTRR 1570 1580 1590 ERSPGRLFED SSRGRLPAGA VRTPLSQVNK VWDQSSV
	Isoform 4 [UniParc]. Checksum: 50944A35251224E9	FASTA	2,012	230,083
	10 20 30 40 50 60 MLKFKYGVRN PPEASASEPI ASRASRLNLF FQGKPPLMTQ QQMSALSREG MLDALFALFE 70 80 90 100 110 120 ECSQPALMKM KHVSSFVQKY SDTIAELREL QPSARDFEVR SLVGCGHFAE VQVVREKATG 130 140 150 160 170 180 DVYAMKIMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNNL YLVMEYQPGG 190 200 210 220 230 240 DFLSLLNRYE DQLDESMIQF YLAELILAVH SVHQMGYVHR DIKPENILID RTGEIKLVDF 250 260 270 280 290 300 GSAAKMNSNK VDAKLPIGTP DYMAPEVLTV MNEDRRGTYG LDCDWWSVGV VAYEMVYGKT 310 320 330 340 350 360 PFTEGTSART FNNIMNFQRF LKFPDDPKVS SELLDLLQSL LCVQKERLKF EGLCCHPFFA 370 380 390 400 410 420 RTDWNNIRNS PPPFVPTLKS DDDTSNFDEP EKNSWVSSSV CQLSPSGFSG EELPFVGFSY 430 440 450 460 470 480 SKALGYLGRS ESVVSSLDSP AKVSSMEKKL LIKSKELQDS QDKCHKMEQE MTRLHRRVSE 490 500 510 520 530 540 VEAVLSQKEV ELKASETQRS LLEQDLATYI TECSSLKRSL EQARMEVSQE DDKALQLLHD 550 560 570 580 590 600 IREQSRKLQE IKEQEYQAQV EEMRLMMNQL EEDLVSARRR SDLYESELRE SRLAAEEFKR 610 620 630 640 650 660 KANECQHKLM KAKDQGKPEV GEYSKLEKIN AEQQLKIQEL QEKLEKAVKA STEATELLQN 670 680 690 700 710 720 IRQAKERAER ELEKLHNRED SSEGIKKKLV EAELEEKHRE AQVSAQHLEV HLKQKEQHYE 730 740 750 760 770 780 EKIKVLDNQI KKDLADKESL ENMMQRHEEE AHEKGKILSE QKAMINAMDS KIRSLEQRIV 790 800 810 820 830 840 ELSEANKLAA NSSLFTQRNM KAQEEMISEL RQQKFYLETQ AGKLEAQNRK LEEQLEKISH 850 860 870 880 890 900 QDHSDKSRLL ELETRLREVS LEHEEQKLEL KRQLTELQLS LQERESQLTA LQAARAALES 910 920 930 940 950 960 QLRQAKTELE ETTAEAEEEI QALTAHRDEI QRKFDALRNS CTVITDLEEQ LNQLTEDNAE 970 980 990 1000 1010 1020 LNNQNFYLSK QLDEASGAND EIVQLRSEVD HLRREITERE MQLTSQKQTM EALKTTCTML 1030 1040 1050 1060 1070 1080 EEQVLDLEAL NDELLEKERQ WEAWRSVLGD EKSQFECRVR ELQRMLDTEK QSRARADQRI 1090 1100 1110 1120 1130 1140 TESRQVVELA VKEHKAEILA LQQALKEQKL KAESLSDKLN DLEKKHAMLE MNARSLQQKL 1150 1160 1170 1180 1190 1200 ETERELKQRL LEEQAKLQQQ MDLQKNHIFR LTQGLQEALD RADLLKTERS DLEYQLENIQ 1210 1220 1230 1240 1250 1260 VLYSHEKVKM EGTISQQTKL IDFLQAKMDQ PAKKKKVPLQ YNELKLALEK EKARCAELEE 1270 1280 1290 1300 1310 1320 ALQKTRIELR SAREEAAHRK ATDHPHPSTP ATARQQIAMS AIVRSPEHQP SAMSLLAPPS 1330 1340 1350 1360 1370 1380 SRRKESSTPE EFSRRLKERM HHNIPHRFNV GLNMRATKCA VCLDTVHFGR QASKCLECQV 1390 1400 1410 1420 1430 1440 MCHPKCSTCL PATCGLPAEY ATHFTEAFCR DKMNSPGLQS KEPGSSLHLE GWMKVPRNNK 1450 1460 1470 1480 1490 1500 RGQQGWDRKY IVLEGSKVLI YDNEAREAGQ RPVEEFELCL PDGDVSIHGA VGASELANTA 1510 1520 1530 1540 1550 1560 KADVPYILKM ESHPHTTCWP GRTLYLLAPS FPDKQRWVTA LESVVAGGRV SREKAEADAK 1570 1580 1590 1600 1610 1620 LLGNSLLKLE GDDRLDMNCT LPFSDQVVLV GTEEGLYALN VLKNSLTHIP GIGAVFQIYI 1630 1640 1650 1660 1670 1680 IKDLEKLLMI AGEERALCLV DVKKVKQSLA QSHLPAQPDV SPNIFEAVKG CHLFAAGKIE 1690 1700 1710 1720 1730 1740 NSLCICAAMP SKVVILRYND NLSKYCIRKE IETSEPCSCI HFTNYSILIG TNKFYEIDMK 1750 1760 1770 1780 1790 1800 QYTLDEFLDK NDHSLAPAVF ASSSNSFPVS IVQANSAGQR EEYLLCFHEF GVFVDSYGRR 1810 1820 1830 1840 1850 1860 SRTDDLKWSR LPLAFAYREP YLFVTHFNSL EVIEIQARSS LGSPARAYLE IPNPRYLGPA 1870 1880 1890 1900 1910 1920 ISSGAIYLAS SYQDKLRVIC CKGNLVKESG TEQHRVPSTS RSSPNKRGPP TYNEHITKRV 1930 1940 1950 1960 1970 1980 ASSPAPPEGP SHPREPSTPH RYRDREGRTE LRRDKSPGRP LEREKSPGRM LSTRRERSPG 1990 2000 2010 RLFEDSSRGR LPAGAVRTPL SQVNKVWDQS SV
	Isoform 5 [UniParc]. Checksum: 996B21DE7CBF45AD	FASTA	2,085	238,846
	10 20 30 40 50 60 MLKFKYGVRN PPEASASEPI ASRASRLNLF FQGKPPLMTQ QQMSALSREG MLDALFALFE 70 80 90 100 110 120 ECSQPALMKM KHVSSFVQKY SDTIAELREL QPSARDFEVR SLVGCGHFAE VQVVREKATG 130 140 150 160 170 180 DVYAMKIMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNNL YLVMEYQPGG 190 200 210 220 230 240 DFLSLLNRYE DQLDESMIQF YLAELILAVH SVHQMGYVHR DIKPENILID RTGEIKLVDF 250 260 270 280 290 300 GSAAKMNSNK VDAKLPIGTP DYMAPEVLTV MNEDRRGTYG LDCDWWSVGV VAYEMVYGKT 310 320 330 340 350 360 PFTEGTSART FNNIMNFQRF LKFPDDPKVS SELLDLLQSL LCVQKERLKF EGLCCHPFFA 370 380 390 400 410 420 RTDWNNIRNS PPPFVPTLKS DDDTSNFDEP EKNSWVSSSV CQLSPSGFSG EELPFVGFSY 430 440 450 460 470 480 SKALGYLGRS ESVVSSLDSP AKVSSMEKKL LIKSKELQDS QDKCHKMEQE MTRLHRRVSE 490 500 510 520 530 540 VEAVLSQKEV ELKASETQRS LLEQDLATYI TECSSLKRSL EQARMEVSQE DDKALQLLHD 550 560 570 580 590 600 IREQSRKLQE IKEQEYQAQV EEMRLMMNQL EEDLVSARRR SDLYESELRE SRLAAEEFKR 610 620 630 640 650 660 KANECQHKLM KAKDQGKPEV GEYSKLEKIN AEQQLKIQEL QEKLEKAVKA STEATELLQN 670 680 690 700 710 720 IRQAKERAER ELEKLHNRED SSEGIKKKLV EAEERRHSLE NKVKRLETME RRENRLKDDI 730 740 750 760 770 780 QTKSEQIQQM ADKILELEEK HREAQVSAQH LEVHLKQKEQ HYEEKIKVLD NQIKKDLADK 790 800 810 820 830 840 ESLENMMQRH EEEAHEKGKI LSEQKAMINA MDSKIRSLEQ RIVELSEANK LAANSSLFTQ 850 860 870 880 890 900 RNMKAQEEMI SELRQQKFYL ETQAGKLEAQ NRKLEEQLEK ISHQDHSDKS RLLELETRLR 910 920 930 940 950 960 EVSLEHEEQK LELKRQLTEL QLSLQERESQ LTALQAARAA LESQLRQAKT ELEETTAEAE 970 980 990 1000 1010 1020 EEIQALTAHR DEIQRKFDAL RNSCTVITDL EEQLNQLTED NAELNNQNFY LSKQLDEASG 1030 1040 1050 1060 1070 1080 ANDEIVQLRS EVDHLRREIT EREMQLTSQK QTMEALKTTC TMLEEQVLDL EALNDELLEK 1090 1100 1110 1120 1130 1140 ERQWEAWRSV LGDEKSQFEC RVRELQRMLD TEKQSRARAD QRITESRQVV ELAVKEHKAE 1150 1160 1170 1180 1190 1200 ILALQQALKE QKLKAESLSD KLNDLEKKHA MLEMNARSLQ QKLETERELK QRLLEEQAKL 1210 1220 1230 1240 1250 1260 QQQMDLQKNH IFRLTQGLQE ALDRADLLKT ERSDLEYQLE NIQVLYSHEK VKMEGTISQQ 1270 1280 1290 1300 1310 1320 TKLIDFLQAK MDQPAKKKKG LFSRRKEDPA LPTQVPLQYN ELKLALEKEK ARCAELEEAL 1330 1340 1350 1360 1370 1380 QKTRIELRSA REEAAHRKAT DHPHPSTPAT ARQQIAMSAI VRSPEHQPSA MSLLAPPSSR 1390 1400 1410 1420 1430 1440 RKESSTPEEF SRRLKERMHH NIPHRFNVGL NMRATKCAVC LDTVHFGRQA SKCLECQVMC 1450 1460 1470 1480 1490 1500 HPKCSTCLPA TCGLPAEYAT HFTEAFCRDK MNSPGLQSKE PGSSLHLEGW MKVPRNNKRG 1510 1520 1530 1540 1550 1560 QQGWDRKYIV LEGSKVLIYD NEAREAGQRP VEEFELCLPD GDVSIHGAVG ASELANTAKA 1570 1580 1590 1600 1610 1620 DVPYILKMES HPHTTCWPGR TLYLLAPSFP DKQRWVTALE SVVAGGRVSR EKAEADAARD 1630 1640 1650 1660 1670 1680 HTSSEHQPVW VEKLLGNSLL KLEGDDRLDM NCTLPFSDQV VLVGTEEGLY ALNVLKNSLT 1690 1700 1710 1720 1730 1740 HIPGIGAVFQ IYIIKDLEKL LMIAGEERAL CLVDVKKVKQ SLAQSHLPAQ PDVSPNIFEA 1750 1760 1770 1780 1790 1800 VKGCHLFAAG KIENSLCICA AMPSKVVILR YNDNLSKYCI RKEIETSEPC SCIHFTNYSI 1810 1820 1830 1840 1850 1860 LIGTNKFYEI DMKQYTLDEF LDKNDHSLAP AVFASSSNSF PVSIVQANSA GQREEYLLCF 1870 1880 1890 1900 1910 1920 HEFGVFVDSY GRRSRTDDLK WSRLPLAFAY REPYLFVTHF NSLEVIEIQA RSSLGSPARA 1930 1940 1950 1960 1970 1980 YLEIPNPRYL GPAISSGAIY LASSYQDKLR VICCKGNLVK ESGTEQHRVP STSRSSPNKR 1990 2000 2010 2020 2030 2040 GPPTYNEHIT KRVASSPAPP EGPSHPREPS TPHRYRDREG RTELRRDKSP GRPLEREKSP 2050 2060 2070 2080 GRMLSTRRER SPGRLFEDSS RGRLPAGAVR TPLSQVNKVW DQSSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel partner for the GTP-bound forms of rho and rac." Madaule P., Furuyashiki T., Reid T., Ishizaki T., Watanabe G., Morii N., Narumiya S. FEBS Lett. 377:243-248(1995) [PubMed: 8543060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION. Tissue: Brain.
[2]	"Citron Rho-interacting kinase, a novel tissue-specific Ser/Thr kinase encompassing the Rho-Rac-binding protein Citron." Di Cunto F., Calautti E., Hsiao J., Ong L., Topley G., Turco E., Dotto G.P. J. Biol. Chem. 273:29706-29711(1998) [PubMed: 9792683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-126. Tissue: Keratinocyte.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Mammary gland.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 246-254, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[5]	"Role of citron kinase as a target of the small GTPase Rho in cytokinesis." Madaule P., Eda M., Watanabe N., Fujisawa K., Matsuoka T., Bito H., Ishizaki T., Narumiya S. Nature 394:491-494(1998) [PubMed: 9697773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 373-2055 (ISOFORM 4), FUNCTION. Tissue: Brain.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2055 (ISOFORM 5). Strain: FVB/N. Tissue: Mammary tumor.
[7]	"Defective neurogenesis in citron kinase knockout mice by altered cytokinesis and massive apoptosis." Di Cunto F., Imarisio S., Hirsch E., Broccoli V., Bulfone A., Migheli A., Atzori C., Turco E., Triolo R., Dotto G.P., Silengo L., Altruda F. Neuron 28:115-127(2000) [PubMed: 11086988] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[8]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, MASS SPECTROMETRY. Tissue: Brain.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1237 AND TYR-1504, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1608, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39904 mRNA. Translation: AAC52341.1. AF086823 mRNA. Translation: AAC72822.1. Frameshift. AF086824 mRNA. Translation: AAC72823.1. Frameshift. AK145037 mRNA. Translation: BAE26199.1. AF070066 mRNA. Translation: AAC27933.1. BC023775 mRNA. Translation: AAH23775.1. Frameshift. BC051165 mRNA. Translation: AAH51165.1. Frameshift.
IPI	IPI00406762. IPI00474730. IPI00655040. IPI00655108. IPI00956788.
PIR	S68420.
UniGene	Mm.426282. Mm.8321.
3D structure databases
ProteinModelPortal	P49025.
SMR	P49025. Positions 44-423, 1389-1437, 1471-1587.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49025. 5 interactions.
STRING	P49025.
PTM databases
PhosphoSite	P49025.
Proteomic databases
PRIDE	P49025.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	uc008zep.1. mouse. uc008zet.1. mouse.
Organism-specific databases
MGI	MGI:105313. Cit.
Phylogenomic databases
eggNOG	roNOG11544.
GeneTree	ENSGT00600000084128.
HOVERGEN	HBG071093.
OrthoDB	EOG402WR8.
PhylomeDB	P49025.
Gene expression databases
ArrayExpress	P49025.
Bgee	P49025.
CleanEx	MM_CIT.
Genevestigator	P49025.
GermOnline	ENSMUSG00000029516. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR001180. Citron. IPR017405. Citron_Rho-interacting_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR017892. Pkinase_C. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00780. CNH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF038145. Citron_Rho-interacting_kinase. 1 hit.
SMART	SM00109. C1. 1 hit. SM00036. CNH. 1 hit. SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50219. CNH. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

CTRO_MOUSE

Accession

Primary (citable) accession number: P49025
Secondary accession number(s): O88528 , O88937, O88938, Q3UM99, Q8CIJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 8, 2005
Last modified:	November 16, 2011
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program