Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Citron Rho-interacting kinase

Gene

Cit

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126ATPPROSITE-ProRule annotation1
Active sitei221Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi103 – 111ATPPROSITE-ProRule annotation9
Zinc fingeri1388 – 1437Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • dendrite development Source: MGI
  • generation of neurons Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • metaphase/anaphase transition of mitotic cell cycle Source: MGI
  • mitotic cell cycle Source: UniProtKB
  • mitotic cytokinesis Source: UniProtKB
  • mitotic sister chromatid segregation Source: MGI
  • negative regulation of dendrite morphogenesis Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • neuron apoptotic process Source: UniProtKB
  • positive regulation of cytokinesis Source: MGI
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Differentiation, Mitosis, Neurogenesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Citron Rho-interacting kinase (EC:2.7.11.1)
Short name:
CRIK
Alternative name(s):
Rho-interacting, serine/threonine-protein kinase 21
Gene namesi
Name:Cit
Synonyms:Crik
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105313 Cit

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death before reaching adulthood, probably due to lethal epilepsy. Mice display severe defects in the olfactory bulbs, the hippocampus, and the cerebellum. These defects appear to result from impaired cytokinesis followed by the induction of apoptosis in specific neuroblast populations.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi126K → A: Loss of phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859091 – 2055Citron Rho-interacting kinaseAdd BLAST2055

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei439PhosphoserineCombined sources1
Modified residuei479PhosphoserineCombined sources1
Modified residuei581PhosphoserineBy similarity1
Modified residuei1237PhosphotyrosineCombined sources1
Modified residuei1747N6-acetyllysineBy similarity1
Modified residuei1966PhosphoserineBy similarity1
Modified residuei2021PhosphoserineBy similarity1
Modified residuei2041PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP49025
PaxDbiP49025
PeptideAtlasiP49025
PRIDEiP49025

PTM databases

iPTMnetiP49025
PhosphoSitePlusiP49025

Expressioni

Tissue specificityi

A major signal was observed in testis and brain, but it was also detected in thymus, spleen, kidney, heart and lung.2 Publications

Developmental stagei

Detected at E10.5 with highest expression in the developing central nervous system. After E16.5 expression decreases and at two weeks after birth is restricted to the proliferating neuronal precursor cells in the external germinal layer of the cerebellum and subventricular migratory stream.1 Publication

Gene expression databases

CleanExiMM_CIT

Interactioni

Subunit structurei

Interacts with TTC3 (By similarity). Homodimer (Probable). Directly interacts with KIF14 depending on the activation state (stronger interaction with the kinase-dead form).By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198720, 21 interactors
IntActiP49025, 10 interactors
MINTiP49025
STRINGi10090.ENSMUSP00000062049

Structurei

3D structure databases

ProteinModelPortaliP49025
SMRiP49025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 359Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini360 – 430AGC-kinase C-terminalAdd BLAST71
Domaini1469 – 1589PHPROSITE-ProRule annotationAdd BLAST121
Domaini1617 – 1907CNHPROSITE-ProRule annotationAdd BLAST291

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1132 – 1328Interaction with Rho/RacAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili441 – 1086Sequence analysisAdd BLAST646
Coiled coili1091 – 1247Sequence analysisAdd BLAST157
Coiled coili1275 – 1325Sequence analysisAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1979 – 1984SH3-bindingSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi550 – 962Glu-richAdd BLAST413
Compositional biasi1276 – 1279Poly-Lys4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1388 – 1437Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, SH3-binding, Zinc-finger

Phylogenomic databases

eggNOGiKOG0612 Eukaryota
KOG0976 Eukaryota
ENOG410XR1Q LUCA
HOGENOMiHOG000015347
HOVERGENiHBG071093
InParanoidiP49025
PhylomeDBiP49025

Family and domain databases

CDDicd00029 C1, 1 hit
cd05601 STKc_CRIK, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR017405 Citron_Rho-interacting_kinase
IPR001180 CNH_dom
IPR037708 CRIK_dom
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22988:SF26 PTHR22988:SF26, 2 hits
PfamiView protein in Pfam
PF00780 CNH, 1 hit
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PIRSFiPIRSF038145 Citron_Rho-interacting_kinase, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00036 CNH, 1 hit
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50219 CNH, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P49025-1) [UniParc]FASTAAdd to basket
Also known as: CRIK

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKFKYGVRN PPEASASEPI ASRASRLNLF FQGKPPLMTQ QQMSALSREG
60 70 80 90 100
MLDALFALFE ECSQPALMKM KHVSSFVQKY SDTIAELREL QPSARDFEVR
110 120 130 140 150
SLVGCGHFAE VQVVREKATG DVYAMKIMKK KALLAQEQVS FFEEERNILS
160 170 180 190 200
RSTSPWIPQL QYAFQDKNNL YLVMEYQPGG DFLSLLNRYE DQLDESMIQF
210 220 230 240 250
YLAELILAVH SVHQMGYVHR DIKPENILID RTGEIKLVDF GSAAKMNSNK
260 270 280 290 300
VDAKLPIGTP DYMAPEVLTV MNEDRRGTYG LDCDWWSVGV VAYEMVYGKT
310 320 330 340 350
PFTEGTSART FNNIMNFQRF LKFPDDPKVS SELLDLLQSL LCVQKERLKF
360 370 380 390 400
EGLCCHPFFA RTDWNNIRNS PPPFVPTLKS DDDTSNFDEP EKNSWVSSSV
410 420 430 440 450
CQLSPSGFSG EELPFVGFSY SKALGYLGRS ESVVSSLDSP AKVSSMEKKL
460 470 480 490 500
LIKSKELQDS QDKCHKMEQE MTRLHRRVSE VEAVLSQKEV ELKASETQRS
510 520 530 540 550
LLEQDLATYI TECSSLKRSL EQARMEVSQE DDKALQLLHD IREQSRKLQE
560 570 580 590 600
IKEQEYQAQV EEMRLMMNQL EEDLVSARRR SDLYESELRE SRLAAEEFKR
610 620 630 640 650
KANECQHKLM KAKDQGKPEV GEYSKLEKIN AEQQLKIQEL QEKLEKAVKA
660 670 680 690 700
STEATELLQN IRQAKERAER ELEKLHNRED SSEGIKKKLV EAEERRHSLE
710 720 730 740 750
NKVKRLETME RRENRLKDDI QTKSEQIQQM ADKILELEEK HREAQVSAQH
760 770 780 790 800
LEVHLKQKEQ HYEEKIKVLD NQIKKDLADK ESLENMMQRH EEEAHEKGKI
810 820 830 840 850
LSEQKAMINA MDSKIRSLEQ RIVELSEANK LAANSSLFTQ RNMKAQEEMI
860 870 880 890 900
SELRQQKFYL ETQAGKLEAQ NRKLEEQLEK ISHQDHSDKS RLLELETRLR
910 920 930 940 950
EVSLEHEEQK LELKRQLTEL QLSLQERESQ LTALQAARAA LESQLRQAKT
960 970 980 990 1000
ELEETTAEAE EEIQALTAHR DEIQRKFDAL RNSCTVITDL EEQLNQLTED
1010 1020 1030 1040 1050
NAELNNQNFY LSKQLDEASG ANDEIVQLRS EVDHLRREIT EREMQLTSQK
1060 1070 1080 1090 1100
QTMEALKTTC TMLEEQVLDL EALNDELLEK ERQWEAWRSV LGDEKSQFEC
1110 1120 1130 1140 1150
RVRELQRMLD TEKQSRARAD QRITESRQVV ELAVKEHKAE ILALQQALKE
1160 1170 1180 1190 1200
QKLKAESLSD KLNDLEKKHA MLEMNARSLQ QKLETERELK QRLLEEQAKL
1210 1220 1230 1240 1250
QQQMDLQKNH IFRLTQGLQE ALDRADLLKT ERSDLEYQLE NIQVLYSHEK
1260 1270 1280 1290 1300
VKMEGTISQQ TKLIDFLQAK MDQPAKKKKV PLQYNELKLA LEKEKARCAE
1310 1320 1330 1340 1350
LEEALQKTRI ELRSAREEAA HRKATDHPHP STPATARQQI AMSAIVRSPE
1360 1370 1380 1390 1400
HQPSAMSLLA PPSSRRKESS TPEEFSRRLK ERMHHNIPHR FNVGLNMRAT
1410 1420 1430 1440 1450
KCAVCLDTVH FGRQASKCLE CQVMCHPKCS TCLPATCGLP AEYATHFTEA
1460 1470 1480 1490 1500
FCRDKMNSPG LQSKEPGSSL HLEGWMKVPR NNKRGQQGWD RKYIVLEGSK
1510 1520 1530 1540 1550
VLIYDNEARE AGQRPVEEFE LCLPDGDVSI HGAVGASELA NTAKADVPYI
1560 1570 1580 1590 1600
LKMESHPHTT CWPGRTLYLL APSFPDKQRW VTALESVVAG GRVSREKAEA
1610 1620 1630 1640 1650
DAKLLGNSLL KLEGDDRLDM NCTLPFSDQV VLVGTEEGLY ALNVLKNSLT
1660 1670 1680 1690 1700
HIPGIGAVFQ IYIIKDLEKL LMIAGEERAL CLVDVKKVKQ SLAQSHLPAQ
1710 1720 1730 1740 1750
PDVSPNIFEA VKGCHLFAAG KIENSLCICA AMPSKVVILR YNDNLSKYCI
1760 1770 1780 1790 1800
RKEIETSEPC SCIHFTNYSI LIGTNKFYEI DMKQYTLDEF LDKNDHSLAP
1810 1820 1830 1840 1850
AVFASSSNSF PVSIVQANSA GQREEYLLCF HEFGVFVDSY GRRSRTDDLK
1860 1870 1880 1890 1900
WSRLPLAFAY REPYLFVTHF NSLEVIEIQA RSSLGSPARA YLEIPNPRYL
1910 1920 1930 1940 1950
GPAISSGAIY LASSYQDKLR VICCKGNLVK ESGTEQHRVP STSRSSPNKR
1960 1970 1980 1990 2000
GPPTYNEHIT KRVASSPAPP EGPSHPREPS TPHRYRDREG RTELRRDKSP
2010 2020 2030 2040 2050
GRPLEREKSP GRMLSTRRER SPGRLFEDSS RGRLPAGAVR TPLSQVNKVW

DQSSV
Length:2,055
Mass (Da):235,389
Last modified:November 8, 2005 - v3
Checksum:iBDA9A12C14E12DF8
GO
Isoform 2 (identifier: P49025-2) [UniParc]FASTAAdd to basket
Also known as: CRIK-SK

The sequence of this isoform differs from the canonical sequence as follows:
     467-494: MEQEMTRLHRRVSEVEAVLSQKEVELKA → VSISTAGLRPCSRILQSIYAEGSAGGHC
     495-2055: Missing.

Show »
Length:494
Mass (Da):55,650
Checksum:iE4AEAEA4C22D0051
GO
Isoform 3 (identifier: P49025-3) [UniParc]FASTAAdd to basket
Also known as: Citron

The sequence of this isoform differs from the canonical sequence as follows:
     1-458: Missing.
     459-466: DSQDKCHK → MLLGEEAM

Show »
Length:1,597
Mass (Da):183,449
Checksum:i7B7286C2305676DA
GO
Isoform 4 (identifier: P49025-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     693-735: Missing.

Show »
Length:2,012
Mass (Da):230,083
Checksum:i50944A35251224E9
GO
Isoform 5 (identifier: P49025-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1279-1279: K → KGLFSRRKEDPALPTQ
     1602-1602: A → AARDHTSSEHQPVWVE

Note: No experimental confirmation available.
Show »
Length:2,085
Mass (Da):238,846
Checksum:i996B21DE7CBF45AD
GO

Sequence cautioni

The sequence AAC72822 differs from that shown. Reason: Frameshift at positions 396 and 408.Curated
The sequence AAC72823 differs from that shown. Reason: Frameshift at positions 396 and 408.Curated
The sequence AAH23775 differs from that shown. Reason: Frameshift at position 1011.Curated
The sequence AAH51165 differs from that shown. Reason: Frameshift at position 1011.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78Q → R in BAE26199 (PubMed:16141072).Curated1
Sequence conflicti182F → L in BAE26199 (PubMed:16141072).Curated1
Sequence conflicti234E → H in BAE26199 (PubMed:16141072).Curated1
Sequence conflicti1945Missing in AAH51165 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0124361 – 458Missing in isoform 3. 1 PublicationAdd BLAST458
Alternative sequenceiVSP_012437459 – 466DSQDKCHK → MLLGEEAM in isoform 3. 1 Publication8
Alternative sequenceiVSP_012438467 – 494MEQEM…VELKA → VSISTAGLRPCSRILQSIYA EGSAGGHC in isoform 2. 2 PublicationsAdd BLAST28
Alternative sequenceiVSP_012439495 – 2055Missing in isoform 2. 2 PublicationsAdd BLAST1561
Alternative sequenceiVSP_016093693 – 735Missing in isoform 4. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_0160941279K → KGLFSRRKEDPALPTQ in isoform 5. 1 Publication1
Alternative sequenceiVSP_0160951602A → AARDHTSSEHQPVWVE in isoform 5. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39904 mRNA Translation: AAC52341.1
AF086823 mRNA Translation: AAC72822.1 Frameshift.
AF086824 mRNA Translation: AAC72823.1 Frameshift.
AK145037 mRNA Translation: BAE26199.1
AF070066 mRNA Translation: AAC27933.1
BC023775 mRNA Translation: AAH23775.1 Frameshift.
BC051165 mRNA Translation: AAH51165.1 Frameshift.
CCDSiCCDS19597.1 [P49025-1]
PIRiS68420
RefSeqiXP_006530214.1, XM_006530151.3 [P49025-3]
UniGeneiMm.426282
Mm.8321

Genome annotation databases

GeneIDi12704
UCSCiuc008zep.1 mouse [P49025-2]
uc008zet.1 mouse [P49025-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCTRO_MOUSE
AccessioniPrimary (citable) accession number: P49025
Secondary accession number(s): O88528
, O88937, O88938, Q3UM99, Q8CIJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health