Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Paxillin

Gene

PXN

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of v-CRK.

GO - Molecular functioni

  • vinculin binding Source: AgBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell-matrix adhesion Source: InterPro
  • response to muscle stretch Source: AgBase
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Paxillin
Gene namesi
Name:PXN
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • cell Source: AgBase
  • cytoplasm Source: UniProtKB-KW
  • focal adhesion Source: AgBase
  • plasma membrane Source: AgBase
  • stress fiber Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559PaxillinPRO_0000075857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311PhosphotyrosineBy similarity
Modified residuei118 – 1181Phosphotyrosine; by FAK11 Publication

Post-translational modificationi

Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49024.

PTM databases

iPTMnetiP49024.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
E6P031262EBI-2896280,EBI-1177242From a different organism.
E6P069315EBI-2896280,EBI-7281937From a different organism.
GIT1Q9Y2X73EBI-2896280,EBI-466061From a different organism.
PTK2Q009442EBI-2896280,EBI-2896409
VCLP120035EBI-2896280,EBI-1039563
VCLP12003-12EBI-2896280,EBI-6138078
VCLP12003-22EBI-2896280,EBI-6138096
VclQ647274EBI-2896280,EBI-432047From a different organism.

GO - Molecular functioni

  • vinculin binding Source: AgBase

Protein-protein interaction databases

BioGridi676106. 4 interactions.
IntActiP49024. 12 interactions.
MINTiMINT-1532065.
STRINGi9031.ENSGALP00000011713.

Structurei

Secondary structure

1
559
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi142 – 15716Combined sources
Helixi262 – 2654Combined sources
Helixi267 – 2759Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L6FNMR-A140-161[»]
A262-275[»]
2L6GNMR-A140-161[»]
2L6HNMR-A263-276[»]
4R32X-ray3.50B/C139-162[»]
ProteinModelPortaliP49024.
SMRiP49024. Positions 434-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 37651LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini385 – 43551LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini444 – 49451LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini503 – 55351LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 538Pro-rich

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
HOGENOMiHOG000018764.
HOVERGENiHBG001512.
InParanoidiP49024.
KOiK05760.
PhylomeDBiP49024.

Family and domain databases

Gene3Di2.10.110.10. 4 hits.
InterProiIPR001904. Paxillin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24216:SF11. PTHR24216:SF11. 1 hit.
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDLDALLAD LESTTSHISK RPVFLTEETP YSYPTGNHTY QEIAVPPPVP
60 70 80 90 100
PPPSSEALNG TVIDPLDQWQ PSVSRYGHQQ PQSQSPIYSS SAKSSSASVP
110 120 130 140 150
RDGLSSPSPR ASEEEHVYSF PNKQKSAEPS PTMTSTSLGS NLSELDRLLL
160 170 180 190 200
ELNAVQHNPP SGFSADEVSR SPSLPNVTGP HYVIPESSSS AGGKAAPPTK
210 220 230 240 250
EKPKRNGGRG IEDVRPSVES LLDELESSVP SPVPAITVSQ GEVSSPQRVN
260 270 280 290 300
ASQQQTRISA SSATRELDEL MASLSDFKFM AQGKAGGSSS PPSTTPKPGS
310 320 330 340 350
QLDTMLGSLQ SDLNKLGVAT VAKGVCGACK KPIAGQVVTA MGKTWHPEHF
360 370 380 390 400
VCTHCQEEIG SRNFFERDGQ PYCEKDYHNL FSPRCYYCNG PILDKVVTAL
410 420 430 440 450
DRTWHPEHFF CAQCGVFFGP EGFHEKDGKA YCRKDYFDMF APKCGGCARA
460 470 480 490 500
ILENYISALN TLWHPECFVC RECFTPFING SFFEHDGQPY CEVHYHERRG
510 520 530 540 550
SLCSGCQKPI TGRCITAMGK KFHPEHFVCA FCLKQLNKGT FKEQNDKPYC

QNCFLKLFC
Length:559
Mass (Da):61,243
Last modified:February 1, 1996 - v1
Checksum:i6450270D90B2DE84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14589 mRNA. Translation: AAC59665.1.
L30099 mRNA. Translation: AAC38018.1.
PIRiB55933.
RefSeqiNP_990315.1. NM_204984.1.
UniGeneiGga.4056.

Genome annotation databases

GeneIDi395832.
KEGGigga:395832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14589 mRNA. Translation: AAC59665.1.
L30099 mRNA. Translation: AAC38018.1.
PIRiB55933.
RefSeqiNP_990315.1. NM_204984.1.
UniGeneiGga.4056.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L6FNMR-A140-161[»]
A262-275[»]
2L6GNMR-A140-161[»]
2L6HNMR-A263-276[»]
4R32X-ray3.50B/C139-162[»]
ProteinModelPortaliP49024.
SMRiP49024. Positions 434-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676106. 4 interactions.
IntActiP49024. 12 interactions.
MINTiMINT-1532065.
STRINGi9031.ENSGALP00000011713.

PTM databases

iPTMnetiP49024.

Proteomic databases

PaxDbiP49024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395832.
KEGGigga:395832.

Organism-specific databases

CTDi5829.

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
HOGENOMiHOG000018764.
HOVERGENiHBG001512.
InParanoidiP49024.
KOiK05760.
PhylomeDBiP49024.

Miscellaneous databases

PROiP49024.

Family and domain databases

Gene3Di2.10.110.10. 4 hits.
InterProiIPR001904. Paxillin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24216:SF11. PTHR24216:SF11. 1 hit.
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAXI_CHICK
AccessioniPrimary (citable) accession number: P49024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.