P49017 (COQ5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
EC=2.1.1.201

Alternative name(s):
Ubiquinone biosynthesis methyltransferase COQ5

Gene names

Name:	COQ5
Synonyms:	DBI56
Ordered Locus Names:	YML110C
ORF Names:	YM8339.09C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).
Catalytic activity	S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol. Ref.4
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis.
Subcellular location	Mitochondrion Ref.4 Ref.5.
Miscellaneous	Present with 8100 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.4. Source: SGD ubiquinone biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from direct assay. Source: SGD
Molecular function	2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-4937,EBI-4937

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 307	288	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial		PRO_0000193361

Sequences

	Sequence		Length	Mass (Da)
	P49017 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: C8951F9C8FFF8F41	FASTA	307	34,685
	10 20 30 40 50 60 MLISSRIVRS SLVNVPLRLS RCFTQAHRAC KEEEVNSPLS SAAEQPEQKY THFGSKTVLK 70 80 90 100 110 120 STKQKLVGDV FSSVANRYDL MNDVMSLGIH RLWKDHFINK LDAGKRPNST TPLNFIDVAG 130 140 150 160 170 180 GSGDIAFGLL DHAESKFGDT ESTMDIVDIN PDMLKEGEKR AMEQGKYFKD PRVRFLVSNG 190 200 210 220 230 240 EKLEEIDSDS KDIYTVSFGI RNFTDIQKGL NTAYRVLKPG GIFYCLEFSK IENPLMDFAY 250 260 270 280 290 300 QQWAKVLPVM GSMIANDYDS YQYLVESIER FPDQETFKSM IEKAGFKSAG YESLTFGICA IHWGIKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis." Barkovich R.J., Shtanko A., Shepherd J.A., Lee P.T., Myles D.C., Tzagoloff A., Clarke C.F. J. Biol. Chem. 272:9182-9188(1997) [PubMed: 9083049] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49210 Genomic DNA. Translation: CAA89108.1. AY693194 Genomic DNA. Translation: AAT93213.1. BK006946 Genomic DNA. Translation: DAA09788.1.
PIR	S53962.
RefSeq	NP_013597.1. NM_001182472.1.
3D structure databases
ProteinModelPortal	P49017.
SMR	P49017. Positions 108-292.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1639N.
IntAct	P49017. 8 interactions.
MINT	MINT-408577.
STRING	P49017.
Proteomic databases
PeptideAtlas	P49017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML110C; YML110C; YML110C.
GeneID	854930.
KEGG	sce:YML110C.
NMPDR	fig|4932.3.peg.4633.
Organism-specific databases
CYGD	YML110c.
SGD	S000004578. COQ5.
Phylogenomic databases
eggNOG	fuNOG04810.
GeneTree	EFGT00050000003356.
HOGENOM	HBG559580.
OMA	PYQYLVE.
OrthoDB	EOG4N605V.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13704.
Gene expression databases
ArrayExpress	P49017.
Genevestigator	P49017.
GermOnline	YML110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. [Graphical view]
KO	K06127.
PANTHER	PTHR10108:SF24. UbiE/COQ5mtfrase. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977959.

Entry information

Entry name

COQ5_YEAST

Accession

Primary (citable) accession number: P49017
Secondary accession number(s): D6W0H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program