P49010 (HEXC_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Chitooligosaccharidolytic beta-N-acetylglucosaminidase EC=3.2.1.52 Alternative name(s): Beta-GlcNAcase Beta-N-acetylhexosaminidase Beta-hexosaminidase
Organism	Bombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier	7091 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Bombycoidea › Bombyciformes › Bombycidae › Bombycinae › Bombyx

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active during metamorphosis to degrade chitin.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chitin catabolic process Inferred from direct assay Ref.1. Source: UniProtKB polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 596	573	Chitooligosaccharidolytic beta-N-acetylglucosaminidase		PRO_0000012013
Amino acid modifications
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	377	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49010 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F302963E94A3A2FF	FASTA	596	68,213
	10 20 30 40 50 60 MWLQAICIYT VFIIIGCGIP TAAEEHSLWR WTCENNRCTK IRNEPENKEP VLSLEACKMF 70 80 90 100 110 120 CDDYGLLWPK PTIETNLGNF LSKINMNTID IQITKQGKSD DLLTAAADRF KTLVSSSVPK 130 140 150 160 170 180 GFSAKAAGKS VTVYLVNENP YIREFSLDMD ESYELYISST SSDKVNATIR GNSFFGVRNG 190 200 210 220 230 240 LETLSQLIVY DDIRNNLLIV RDVTIKDRPV YPYRGILLDT ARNFYSIDSI KRTIDAMAAV 250 260 270 280 290 300 KLNTFHWHIT DSQSFPLVLQ KRPNLSKLGA YSPTKVYTKQ DIREVVEYGL ERGVRVLPEF 310 320 330 340 350 360 DAPAHVGEGW QDTGLTVCFK AEPWTKFCVE PPCGQLNPTK EELYDYLEDI YVEMAEAFES 370 380 390 400 410 420 TDMFHMGGDE VSERCWNSSE EIQNFMIQNR WNLDKSSFLK LWNYFQKNAQ DRAYKAFGKR 430 440 450 460 470 480 LPLILWTSTL TDYTHVEKFL DKDEYIIQVW TTGADPQIQG LLQKGYRLIM SNYDALYFDC 490 500 510 520 530 540 GFGAWVGSGN NWCSPYIGGQ KVYGNSPAVM ALSYRDQILG GEVALWSEQS DPATLDGRLW 550 560 570 580 590 PRAAAFAERM WAEPSTAWQD AEHRMLHVRE RLVRMGIQAE SLEPEWCYQN QGLCYG

References

[1]

"Purification of a chitooligosaccharidolytic beta-N-acetylglucosaminidase from Bombyx mori larvae during metamorphosis and the nucleotide sequence of its cDNA." Nagamatsu Y., Yanagisawa I., Kimoto M., Okamoto E., Koga D. Biosci. Biotechnol. Biochem. 59:219-225(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Kinshu X Showa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S77548 mRNA. Translation: AAC60521.1.
PIR	JC2539.
RefSeq	NP_001037466.1. NM_001044001.1.
UniGene	Bmo.345.
3D structure databases
ProteinModelPortal	P49010.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	693032.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEXC_BOMMO

Accession

Primary (citable) accession number: P49010

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)