P49009 (HEXA_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase subunit alpha
EC=3.2.1.52

Alternative name(s):
Beta-GlcNAcase subunit alpha
Beta-N-acetylhexosaminidase subunit alpha
N-acetyl-beta-glucosaminidase subunit alpha

Gene names

Name:	HEXA
Synonyms:	BEX-1, HEX-A1

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hexoaminidase complex may contribute to amoebic pathogenicity and may be involved in the destruction of extracellular matrix components. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Cytoplasmic granule Ref.1.
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.
Sequence caution	The sequence AAA80165.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 14	14	By similarity
	Chain	15 – 564	550	Beta-hexosaminidase subunit alpha		PRO_0000012011
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	P49009 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 8EFE1D34609C86A4	FASTA	564	63,935
	10 20 30 40 50 60 MLFIFIVFIS SVFAGNGLNV QNQLLLMPYP SSVSFQWKSP LAIALTSSIQ LNVKSTCNTD 70 80 90 100 110 120 CMNFLKSNFN HTISFPLQQQ TGLQDFKVSL FKEIDLPRIT PSVSSVITDV VVELSSSNPM 130 140 150 160 170 180 PKLQIGFDES YILEVTTNSI SIKAVTVYGA RHAFETLLQL IRISSNKFVI SQLPIKISDA 190 200 210 220 230 240 PRFKWRGLMV DPSRNPLSPL MFKRIIDTLA SVKANVLHIH LSDAQTFVFE SKKYPLLHQK 250 260 270 280 290 300 GMYDESFVLT QSFLRELAQY GANRGVIVYG EIDTPAHTAS WNLGYPGVVA NCWDYIVSTS 310 320 330 340 350 360 MRYGENVLSL NPANPNTFPI IDALMKELSD TFGTDYVHVG GDEVWTSGWS KSKEYSDIQK 370 380 390 400 410 420 FMKSKGLNSL TELEGYFNKY AQEQVIHNGK HPVVWEEVFK KGNADKNTII QVWDDIRLLQ 430 440 450 460 470 480 QVVNSGYKAI FSAGFYLDKQ MPLCNSYDSS TCVNTHSMWV WTNRDMYDND PVKSLSSSEK 490 500 510 520 530 540 ENVLGGEGCS WGESTDEQNF FDRVFQRYSA IAERLWSKES VVDKESHEVR ANYLRCLDVR 550 560 RDIMKGTGPL YHSFCQLPKK EKSN

References

[1]	"The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granules." Riekenberg S., Flockenhaus B., Vahrmann A., Mueller M.C.M., Leippe M., Kiess M., Scholze H.H. Mol. Biochem. Parasitol. 138:217-225(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH HEXB. Strain: ATCC 30459 / HM-1:IMSS.
[2]	Scholze H.H. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 30459 / HM-1:IMSS.
[3]	"The primary structure of an Entamoeba histolytica beta-hexosaminidase A subunit." Beanan M.J., Bailey G.B. J. Eukaryot. Microbiol. 42:632-636(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-564. Strain: ATCC 30459 / HM-1:IMSS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ582954 Genomic DNA. Translation: CAE46968.1. AJ634053 Genomic DNA. Translation: CAG23943.1. U09735 mRNA. Translation: AAA80165.1. Different initiation.
3D structure databases
ProteinModelPortal	P49009.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG3525.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEXA_ENTHI

Accession

Primary (citable) accession number: P49009
Secondary accession number(s): Q70FJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)