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P49009 (HEXA_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase subunit alpha

EC=3.2.1.52
Alternative name(s):
Beta-GlcNAcase subunit alpha
Beta-N-acetylhexosaminidase subunit alpha
N-acetyl-beta-glucosaminidase subunit alpha
Gene names
Name:HEXA
Synonyms:BEX-1, HEX-A1
OrganismEntamoeba histolytica
Taxonomic identifier5759 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hexoaminidase complex may contribute to amoebic pathogenicity and may be involved in the destruction of extracellular matrix components. Ref.1

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Subcellular location

Cytoplasmic granule Ref.1.

Post-translational modification

Glycosylated. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Sequence caution

The sequence AAA80165.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-N-acetylhexosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 By similarity
Chain15 – 564550Beta-hexosaminidase subunit alpha
PRO_0000012011

Amino acid modifications

Glycosylation701N-linked (GlcNAc...) Probable

Sequences

Sequence LengthMass (Da)Tools
P49009 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 8EFE1D34609C86A4

FASTA56463,935
        10         20         30         40         50         60 
MLFIFIVFIS SVFAGNGLNV QNQLLLMPYP SSVSFQWKSP LAIALTSSIQ LNVKSTCNTD 

        70         80         90        100        110        120 
CMNFLKSNFN HTISFPLQQQ TGLQDFKVSL FKEIDLPRIT PSVSSVITDV VVELSSSNPM 

       130        140        150        160        170        180 
PKLQIGFDES YILEVTTNSI SIKAVTVYGA RHAFETLLQL IRISSNKFVI SQLPIKISDA 

       190        200        210        220        230        240 
PRFKWRGLMV DPSRNPLSPL MFKRIIDTLA SVKANVLHIH LSDAQTFVFE SKKYPLLHQK 

       250        260        270        280        290        300 
GMYDESFVLT QSFLRELAQY GANRGVIVYG EIDTPAHTAS WNLGYPGVVA NCWDYIVSTS 

       310        320        330        340        350        360 
MRYGENVLSL NPANPNTFPI IDALMKELSD TFGTDYVHVG GDEVWTSGWS KSKEYSDIQK 

       370        380        390        400        410        420 
FMKSKGLNSL TELEGYFNKY AQEQVIHNGK HPVVWEEVFK KGNADKNTII QVWDDIRLLQ 

       430        440        450        460        470        480 
QVVNSGYKAI FSAGFYLDKQ MPLCNSYDSS TCVNTHSMWV WTNRDMYDND PVKSLSSSEK 

       490        500        510        520        530        540 
ENVLGGEGCS WGESTDEQNF FDRVFQRYSA IAERLWSKES VVDKESHEVR ANYLRCLDVR 

       550        560 
RDIMKGTGPL YHSFCQLPKK EKSN 

« Hide

References

[1]"The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granules."
Riekenberg S., Flockenhaus B., Vahrmann A., Mueller M.C.M., Leippe M., Kiess M., Scholze H.H.
Mol. Biochem. Parasitol. 138:217-225(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH HEXB.
Strain: ATCC 30459 / HM-1:IMSS.
[2]Scholze H.H.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 30459 / HM-1:IMSS.
[3]"The primary structure of an Entamoeba histolytica beta-hexosaminidase A subunit."
Beanan M.J., Bailey G.B.
J. Eukaryot. Microbiol. 42:632-636(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-564.
Strain: ATCC 30459 / HM-1:IMSS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ582954 Genomic DNA. Translation: CAE46968.1.
AJ634053 Genomic DNA. Translation: CAG23943.1.
U09735 mRNA. Translation: AAA80165.1. Different initiation.

3D structure databases

ProteinModelPortalP49009.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3525.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR025705. Beta_hexosaminidase_sua/sub.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00728. Glyco_hydro_20. 1 hit.
[Graphical view]
PIRSFPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSPR00738. GLHYDRLASE20.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEXA_ENTHI
AccessionPrimary (citable) accession number: P49009
Secondary accession number(s): Q70FJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: October 16, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries