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Protein

Beta-hexosaminidase subunit alpha

Gene

HEXA

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hexoaminidase complex may contribute to amoebic pathogenicity and may be involved in the destruction of extracellular matrix components.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase subunit alpha (EC:3.2.1.52)
Alternative name(s):
Beta-GlcNAcase subunit alpha
Beta-N-acetylhexosaminidase subunit alpha
N-acetyl-beta-glucosaminidase subunit alpha
Gene namesi
Name:HEXA
Synonyms:BEX-1, HEX-A1
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Subcellular locationi

Cytoplasmic granule 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414By similarityAdd
BLAST
Chaini15 – 564550Beta-hexosaminidase subunit alphaPRO_0000012011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi70 – 701N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Structurei

3D structure databases

ProteinModelPortaliP49009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3525.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49009-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFIFIVFIS SVFAGNGLNV QNQLLLMPYP SSVSFQWKSP LAIALTSSIQ
60 70 80 90 100
LNVKSTCNTD CMNFLKSNFN HTISFPLQQQ TGLQDFKVSL FKEIDLPRIT
110 120 130 140 150
PSVSSVITDV VVELSSSNPM PKLQIGFDES YILEVTTNSI SIKAVTVYGA
160 170 180 190 200
RHAFETLLQL IRISSNKFVI SQLPIKISDA PRFKWRGLMV DPSRNPLSPL
210 220 230 240 250
MFKRIIDTLA SVKANVLHIH LSDAQTFVFE SKKYPLLHQK GMYDESFVLT
260 270 280 290 300
QSFLRELAQY GANRGVIVYG EIDTPAHTAS WNLGYPGVVA NCWDYIVSTS
310 320 330 340 350
MRYGENVLSL NPANPNTFPI IDALMKELSD TFGTDYVHVG GDEVWTSGWS
360 370 380 390 400
KSKEYSDIQK FMKSKGLNSL TELEGYFNKY AQEQVIHNGK HPVVWEEVFK
410 420 430 440 450
KGNADKNTII QVWDDIRLLQ QVVNSGYKAI FSAGFYLDKQ MPLCNSYDSS
460 470 480 490 500
TCVNTHSMWV WTNRDMYDND PVKSLSSSEK ENVLGGEGCS WGESTDEQNF
510 520 530 540 550
FDRVFQRYSA IAERLWSKES VVDKESHEVR ANYLRCLDVR RDIMKGTGPL
560
YHSFCQLPKK EKSN
Length:564
Mass (Da):63,935
Last modified:March 15, 2005 - v2
Checksum:i8EFE1D34609C86A4
GO

Sequence cautioni

The sequence AAA80165.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ582954 Genomic DNA. Translation: CAE46968.1.
AJ634053 Genomic DNA. Translation: CAG23943.1.
U09735 mRNA. Translation: AAA80165.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ582954 Genomic DNA. Translation: CAE46968.1.
AJ634053 Genomic DNA. Translation: CAG23943.1.
U09735 mRNA. Translation: AAA80165.1. Different initiation.

3D structure databases

ProteinModelPortaliP49009.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG3525.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The beta-N-acetylhexosaminidase of Entamoeba histolytica is composed of two homologous chains and has been localized to cytoplasmic granules."
    Riekenberg S., Flockenhaus B., Vahrmann A., Mueller M.C.M., Leippe M., Kiess M., Scholze H.H.
    Mol. Biochem. Parasitol. 138:217-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH HEXB.
    Strain: ATCC 30459 / HM-1:IMSS.
  2. Scholze H.H.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 30459 / HM-1:IMSS.
  3. "The primary structure of an Entamoeba histolytica beta-hexosaminidase A subunit."
    Beanan M.J., Bailey G.B.
    J. Eukaryot. Microbiol. 42:632-636(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-564.
    Strain: ATCC 30459 / HM-1:IMSS.

Entry informationi

Entry nameiHEXA_ENTHI
AccessioniPrimary (citable) accession number: P49009
Secondary accession number(s): Q70FJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: October 1, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.