P49008 (HEXA_PORGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-GlcNAcase
Beta-N-acetylhexosaminidase
Short name=Beta-NAHase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nahA
Ordered Locus Names:	PG_0043

Organism

Porphyromonas gingivalis (strain ATCC BAA-308 / W83) [Complete proteome] [HAMAP]

Taxonomic identifier

242619 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Cell outer membrane; Lipid-anchor Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 777	759	Beta-hexosaminidase		PRO_0000012016
Amino acid modifications
	Lipidation	19	1	N-palmitoyl cysteine Probable
	Lipidation	19	1	S-diacylglycerol cysteine Probable
Experimental info
	Sequence conflict	258	1	R → H in CAA55582. Ref.1
	Sequence conflict	265	1	E → M in CAA55582. Ref.1
	Sequence conflict	282 – 283	2	LA → FR in CAA55582. Ref.1
	Sequence conflict	575	1	T → S in CAA55582. Ref.1
	Sequence conflict	747	1	G → A in CAA55582. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49008 [UniParc]. Last modified October 3, 2003. Version 2. Checksum: D0A55D2C2FFAD864	FASTA	777	87,661
	10 20 30 40 50 60 MKRLTFGACI CCLLSLMACS QKAKQVQIPE YDKGINIIPL PMQLTESDDS FEVDDKTTIC 70 80 90 100 110 120 VSAEELKPIA KLLADKLRAS ADLSLQIEIG EEPSGNAIYI GVDTALPLKE EGYMLRSDKR 130 140 150 160 170 180 GVSIIGKSAH GAFYGMQTLL QLLPAEVESS NEVLLPMTVP GVEIKDEPAF GYRGFMLDVC 190 200 210 220 230 240 RHFLSVEDIK KHIDIMAMFK INRFHWHLTE DQAWRIEIKK YPRLTEVGST RTEGDGTQYS 250 260 270 280 290 300 GFYTQEQVRD IVQYASDRFI TVIPEIEMPG HAMAALAAYP QLACFPREFK PRIIWGVEQD 310 320 330 340 350 360 VYCAGKDSVF RFISDVIDEV APLFPGTYFH IGGDECPKDR WKACSLCQKR MRDNGLKDEH 370 380 390 400 410 420 ELQSYFIKQA EKVLQKHGKR LIGWDEILEG GLAPSATVMS WRGEDGGIAA ANMNHDVIMT 430 440 450 460 470 480 PGSGGLYLDH YQGDPTVEPV AIGGYAPLEQ VYAYNPLPKE LPADKHRYVL GAQANLWAEY 490 500 510 520 530 540 LYTSERYDYQ AYPRLLAVAE LTWTPLAKKD FADFCRRLDN ACVRLDMHGI NYHIPLPEQP 550 560 570 580 590 600 GGSSDFIAFT DKAKLTFTTS RPMKMVYTLD ETEPTLTSTP YTVPLEFAQT GLLKIRTVTA 610 620 630 640 650 660 GGKMSPVRRI RVEKQPFNMS MEVPAPKPGL TIRTAYGDLY DVPDLQQVAS WEVGTVSSLE 670 680 690 700 710 720 EIMHGKEKIT SPEVLERRVV EATGYVLIPE DGVYEFSTEN NEFWIDNVKL IDNVGEVKKF 730 740 750 760 770 SRRNSSRALQ KGYHPIKTIW VGAIQGGWPT YWNYSRVMIR LKGEEKFKPI SSDMLFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression in Escherichia coli of the nahA gene from Porphyromonas gingivalis indicates that beta-N-acetylhexosaminidase is an outer-membrane-associated lipoprotein." Lovatt A., Roberts I.S. Microbiology 140:3399-3406(1994) [PubMed: 7881557] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-308 / W83.
[2]	"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83." Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M. J. Bacteriol. 185:5591-5601(2003) [PubMed: 12949112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-308 / W83.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78979 Genomic DNA. Translation: CAA55582.1. AE015924 Genomic DNA. Translation: AAQ65295.1.
RefSeq	NP_904396.1. NC_002950.2.
3D structure databases
ProteinModelPortal	P49008.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2552216.
GenomeReviews	Gene locus PG_0043 in contig AE015924_GR.
KEGG	pgi:PG0043.
NMPDR	fig|242619.1.peg.37.
PATRIC	22977240. VBIPorGin134034_0039.
TIGR	PG_0043.
Phylogenomic databases
HOGENOM	HBG649706.
OMA	HIDIMAM.
PhylomeDB	P49008.
ProtClustDB	CLSK826754.
Enzyme and pathway databases
BioCyc	PGIN242619:PG_0043-MONOMER.
Family and domain databases
InterPro	IPR015882. Acetylhexosaminidase_sua/b. IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR011658. PA14. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K12373.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. PF07691. PA14. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
SMART	SM00758. PA14. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEXA_PORGI

Accession

Primary (citable) accession number: P49008

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 3, 2003
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program