P49007 (HEXB_PSEO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase B
EC=3.2.1.52

Alternative name(s):
Beta-GlcNAcase
Beta-N-acetylhexosaminidase
Short name=Beta-NAHase
N-acetyl-beta-glucosaminidase

Gene names

Name:

nag096

Organism

Pseudoalteromonas piscicida

Taxonomic identifier

43662 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 773	754	Beta-hexosaminidase B		PRO_0000012015
Sites
	Active site	531	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 53		By similarity
	Disulfide bond	389 ↔ 397		By similarity
	Disulfide bond	496 ↔ 542		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49007 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DBB379885E004642	FASTA	773	86,760
	10 20 30 40 50 60 MKFNRLMALL FGVSSPLYAL DQTAVNWLGQ NLDVKYTLLD TKPTTCPKAQ QKCYYSELSF 70 80 90 100 110 120 SVRKENTKAN NDFAIFFSQL MPIYHVEGDN FAITHINGDI HKITPAAGFS GFSSAPTTVR 130 140 150 160 170 180 FYTKDSQVTR SEVYAKLCCE RTRSLKLTPQ VIKSTQTQRD NDTGLDCNLN LTPFVTLNQL 190 200 210 220 230 240 QTSSKDDTPW MGSEYLYQHQ VKPTLDAAIG LIPKPKQLTV LSDKRLNLAA GINLQLSGIS 250 260 270 280 290 300 ADAIAMAQQR LNTLGVKSTK EGLVVNVAVK PNKQSSPHYQ LTVAENNISI QGNNSAAAFY 310 320 330 340 350 360 ALQSLAGLLD INDLRIPMVD IIDTPRYDFR GLHVDVARNF RSKAFILQTI EQMAAYKLNK 370 380 390 400 410 420 LHLHLADDEG WRLAIDGLDE LTSVGAYRCF DLTETRCLLP QLGAGNDKNA QVNGFYSAED 430 440 450 460 470 480 YIEILRYAKA HHIEVLPSLD MPGHSRAAII AMEARYKKLM AQGKPEDAQK YRLVETADKT 490 500 510 520 530 540 RYSSIQHYND NTLNVCIANT YTFIDKVLSE VKVLHDRAGV PLNTYHIGAD ETAVLWLESP 550 560 570 580 590 600 ACKKLQASVK DFTNFNGYFI ERVAKLLDKK GIQVAGWSDG LGDVRAANMP ANIQSNGLGD 610 620 630 640 650 660 IKRKRAPVAH RFANQGWQVV LSSPDVTYFD FPYQSHPEER GNHWASRAIE SKKMFEFMPD 670 680 690 700 710 720 NLPAHAEIWK NTNNHAYIAN DSDSSLNKGV QFAGLQGHLW SEMLRSDAQA EYMLYPRLLA 730 740 750 760 770 LAERAWHHAE WELPYQAGRI YSQSSGYFTA KLQAQREADW QRFVAILGNS RTT

References

[1]

"Molecular cloning of the gene which encodes beta-N-acetylglucosaminidase from a marine bacterium, Alteromonas sp. strain O-7." Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Kimura Y., Imada C., Okami Y., Inamori Y. Appl. Environ. Microbiol. 61:804-806(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O-7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D29665 Genomic DNA. Translation: BAA06136.1.
3D structure databases
ProteinModelPortal	P49007.
ModBase	Search...
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.290. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR008965. Carb-bd_dom. IPR012291. CBD_carb-bd_dom. IPR004866. CHB/HEX_N_dom. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF03173. CHB_HEX. 1 hit. PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
SMART	SM01081. CHB_HEX. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEXB_PSEO7

Accession

Primary (citable) accession number: P49007

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program