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Protein

Arachidonate 5-lipoxygenase

Gene

Alox5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.1 Publication

Catalytic activityi

Arachidonate + O2 = leukotriene A4 + H2O.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation
  • Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Pathwayi: leukotriene A4 biosynthesis

This protein is involved in the pathway leukotriene A4 biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway leukotriene A4 biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Calcium 1; via carbonyl oxygen; structuralBy similarity1
Metal bindingi18Calcium 2; via carbonyl oxygen; structuralBy similarity1
Metal bindingi19Calcium 2; structuralBy similarity1
Metal bindingi44Calcium 2; structuralBy similarity1
Metal bindingi45Calcium 2; via carbonyl oxygen; structuralBy similarity1
Metal bindingi47Calcium 2; structuralBy similarity1
Metal bindingi79Calcium 1; via carbonyl oxygen; structuralBy similarity1
Metal bindingi80Calcium 1; via carbonyl oxygen; structuralBy similarity1
Sitei103Essential for stabilizing binding to COTL1By similarity1
Metal bindingi368Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi373Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi551Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi555Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi674Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • inflammatory response Source: MGI
  • leukotriene biosynthetic process Source: MGI
  • leukotriene metabolic process Source: MGI
  • leukotriene production involved in inflammatory response Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Leukotriene biosynthesis

Keywords - Ligandi

Calcium, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.34. 3474.
ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).
R-MMU-6798695. Neutrophil degranulation.
UniPathwayiUPA00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase (EC:1.13.11.34)
Short name:
5-LO
Short name:
5-lipoxygenase
Gene namesi
Name:Alox5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:87999. Alox5.

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation1 Publication
  • Nucleus matrix 1 Publication
  • Nucleus membrane By similarity; Peripheral membrane protein By similarity

  • Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • extracellular space Source: MGI
  • nuclear envelope Source: MGI
  • nuclear envelope lumen Source: UniProtKB
  • nuclear matrix Source: MGI
  • nuclear membrane Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi672V → M: Loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5211.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206951 – 674Arachidonate 5-lipoxygenaseAdd BLAST674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1

Post-translational modificationi

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-524 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48999.
PaxDbiP48999.
PeptideAtlasiP48999.
PRIDEiP48999.

PTM databases

iPTMnetiP48999.
PhosphoSitePlusiP48999.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025701.
CleanExiMM_ALOX5.
ExpressionAtlasiP48999. baseline and differential.
GenevisibleiP48999. MM.

Interactioni

Subunit structurei

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi198076. 1 interactor.
STRINGi10090.ENSMUSP00000026795.

Chemistry databases

BindingDBiP48999.

Structurei

3D structure databases

ProteinModelPortaliP48999.
SMRiP48999.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 118PLATPROSITE-ProRule annotationAdd BLAST117
Domaini119 – 674LipoxygenasePROSITE-ProRule annotationAdd BLAST556

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF0U. Eukaryota.
ENOG410YN4N. LUCA.
GeneTreeiENSGT00550000074415.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiP48999.
KOiK00461.
OMAiMQHWKED.
OrthoDBiEOG091G04A4.
TreeFamiTF105320.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGA
60 70 80 90 100
VDSYDVTVDE ELGEIYLVKI EKRKYWLHDD WYLKYITLKT PHGDYIEFPC
110 120 130 140 150
YRWITGEGEI VLRDGRAKLA RDDQIHILKQ HRRKELEARQ KQYRWMEWNP
160 170 180 190 200
GFPLSIDAKC HKDLPRDIQF DSEKGVDFVL NYSKAMENLF INRFMHMFQS
210 220 230 240 250
SWHDFADFEK IFVKISNTIS ERVKNHWQED LMFGYQFLNG CNPVLIKRCT
260 270 280 290 300
ALPPKLPVTT EMVECSLERQ LSLEQEVQEG NIFIVDYELL DGIDANKTDP
310 320 330 340 350
CTHQFLAAPI CLLYKNLANK IVPIAIQLNQ TPGESNPIFL PTDSKYDWLL
360 370 380 390 400
AKIWVRSSDF HVHQTITHLL RTHLVSEVFG IAMYRQLPAV HPLFKLLVAH
410 420 430 440 450
VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC
460 470 480 490 500
FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV
510 520 530 540 550
EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKLSEYL TVVIFTASAQ
560 570 580 590 600
HAAVNFGQYD WCSWIPNAPP TMRAPPPTAK GVVTIEQIVD TLPDRGRSCW
610 620 630 640 650
HLGAVWALSQ FQENELFLGM YPEEHFIEKP VKEAMIRFRK NLEAIVSVIA
660 670
ERNKNKKLPY YYLSPDRIPN SVAI
Length:674
Mass (Da):77,967
Last modified:July 27, 2011 - v3
Checksum:i130F27F9A77A3D88
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti466I → M in AAC37673 (PubMed:7629107).Curated1
Sequence conflicti646V → I in AAC37673 (PubMed:7629107).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42198 mRNA. Translation: AAC37673.1.
AK137481 mRNA. Translation: BAE23373.1.
AK171413 mRNA. Translation: BAE42439.1.
BC139102 mRNA. Translation: AAI39103.1.
BC141213 mRNA. Translation: AAI41214.1.
CCDSiCCDS20452.1.
PIRiI49479.
RefSeqiNP_033792.1. NM_009662.2.
UniGeneiMm.41072.

Genome annotation databases

EnsembliENSMUST00000026795; ENSMUSP00000026795; ENSMUSG00000025701.
GeneIDi11689.
KEGGimmu:11689.
UCSCiuc009dkd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42198 mRNA. Translation: AAC37673.1.
AK137481 mRNA. Translation: BAE23373.1.
AK171413 mRNA. Translation: BAE42439.1.
BC139102 mRNA. Translation: AAI39103.1.
BC141213 mRNA. Translation: AAI41214.1.
CCDSiCCDS20452.1.
PIRiI49479.
RefSeqiNP_033792.1. NM_009662.2.
UniGeneiMm.41072.

3D structure databases

ProteinModelPortaliP48999.
SMRiP48999.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198076. 1 interactor.
STRINGi10090.ENSMUSP00000026795.

Chemistry databases

BindingDBiP48999.
ChEMBLiCHEMBL5211.

PTM databases

iPTMnetiP48999.
PhosphoSitePlusiP48999.

Proteomic databases

MaxQBiP48999.
PaxDbiP48999.
PeptideAtlasiP48999.
PRIDEiP48999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026795; ENSMUSP00000026795; ENSMUSG00000025701.
GeneIDi11689.
KEGGimmu:11689.
UCSCiuc009dkd.1. mouse.

Organism-specific databases

CTDi240.
MGIiMGI:87999. Alox5.

Phylogenomic databases

eggNOGiENOG410IF0U. Eukaryota.
ENOG410YN4N. LUCA.
GeneTreeiENSGT00550000074415.
HOGENOMiHOG000234358.
HOVERGENiHBG005150.
InParanoidiP48999.
KOiK00461.
OMAiMQHWKED.
OrthoDBiEOG091G04A4.
TreeFamiTF105320.

Enzyme and pathway databases

UniPathwayiUPA00877.
BRENDAi1.13.11.34. 3474.
ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP48999.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025701.
CleanExiMM_ALOX5.
ExpressionAtlasiP48999. baseline and differential.
GenevisibleiP48999. MM.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX5_MOUSE
AccessioniPrimary (citable) accession number: P48999
Secondary accession number(s): Q3TB75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.