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Protein

Transient-receptor-potential-like protein

Gene

trpl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A light-sensitive calcium channel that is required for inositide-mediated Ca2+ entry in the retina during phospholipase C (PLC)-mediated phototransduction. Required for vision in the dark and in dim light. Binds calmodulin. Trp and trpl act together in the light response, although it is unclear whether as heteromultimers or distinct units. Also forms a functional cation channel with Trpgamma. Activated by fatty acids, metabolic stress, inositols and GTP-binding proteins.5 Publications

GO - Molecular functioni

  • calcium channel activity Source: FlyBase
  • calmodulin binding Source: UniProtKB
  • cation channel activity Source: FlyBase
  • identical protein binding Source: FlyBase
  • ion transmembrane transporter activity Source: UniProtKB
  • light-activated ion channel activity Source: FlyBase
  • protein heterodimerization activity Source: UniProtKB
  • store-operated calcium channel activity Source: GO_Central

GO - Biological processi

  • body fluid secretion Source: FlyBase
  • calcium ion transmembrane transport Source: FlyBase
  • calcium ion transport Source: UniProtKB
  • calcium-mediated signaling Source: FlyBase
  • cation transport Source: FlyBase
  • cellular response to anoxia Source: FlyBase
  • detection of light stimulus involved in visual perception Source: UniProtKB
  • ion transport Source: UniProtKB
  • manganese ion transport Source: GO_Central
  • phototransduction, visible light Source: FlyBase
  • regulation of cytosolic calcium ion concentration Source: GO_Central
  • response to light stimulus Source: FlyBase
  • sensory perception of sound Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

Calcium, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-DME-3295583. TRP channels.
R-DME-5578775. Ion homeostasis.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiP48994.

Protein family/group databases

TCDBi1.A.4.1.8. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient-receptor-potential-like protein
Gene namesi
Name:trpl
ORF Names:CG18345
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0005614. trpl.

Subcellular locationi

  • Membrane 2 Publications; Multi-pass membrane protein 2 Publications

  • Note: In the dark, there is 20 fold more rhabdomeral trpl protein forming plasma membrane channels than in the light. In the light, the protein translocates to an intracellular compartment. Protein levels remain unchanged in light and dark conditions.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 340CytoplasmicSequence analysisAdd BLAST340
Transmembranei341 – 361HelicalSequence analysisAdd BLAST21
Topological domaini362 – 373ExtracellularSequence analysisAdd BLAST12
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Topological domaini395 – 431CytoplasmicSequence analysisAdd BLAST37
Transmembranei432 – 452HelicalSequence analysisAdd BLAST21
Topological domaini453 – 512ExtracellularSequence analysisAdd BLAST60
Transmembranei513 – 533HelicalSequence analysisAdd BLAST21
Topological domaini534 – 548CytoplasmicSequence analysisAdd BLAST15
Transmembranei549 – 569HelicalSequence analysisAdd BLAST21
Topological domaini570 – 645ExtracellularSequence analysisAdd BLAST76
Transmembranei646 – 666HelicalSequence analysisAdd BLAST21
Topological domaini667 – 1124CytoplasmicSequence analysisAdd BLAST458

GO - Cellular componenti

  • cation channel complex Source: FlyBase
  • dendrite Source: FlyBase
  • inaD signaling complex Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • intracellular Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • rhabdomere Source: FlyBase
  • rhabdomere microvillus membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi702P → Q: Abolishes interaction with FKBP59. 1 Publication1
Mutagenesisi709P → Q: Abolishes interaction with FKBP59. 1 Publication1
Mutagenesisi713W → G: Disrupts Ca(2+) inflow through the channel. Calmodulin has little effect on Ca(2+) flow. 1 Publication1
Mutagenesisi814W → G: Does not abolish Ca(2+) inflow through the channel. Calmodulin has no effect on initial rates. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153591 – 1124Transient-receptor-potential-like proteinAdd BLAST1124

Proteomic databases

PaxDbiP48994.
PRIDEiP48994.

Expressioni

Tissue specificityi

Expressed predominantly in the rhabdomeres of photoreceptor cells.1 Publication

Gene expression databases

BgeeiFBgn0005614.
GenevisibleiP48994. DM.

Interactioni

Subunit structurei

Forms heteromultimers with Trpgamma and, to a lower extent, with trp. Interacts with FKBP59 in vivo and is found in the inaD signaling complex.3 Publications

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB
  • identical protein binding Source: FlyBase
  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi61834. 4 interactors.
IntActiP48994. 2 interactors.
STRINGi7227.FBpp0290876.

Structurei

3D structure databases

ProteinModelPortaliP48994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati40 – 69ANK 1Add BLAST30
Repeati78 – 107ANK 2Add BLAST30
Repeati152 – 181ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni710 – 728Calmodulin-binding 1Add BLAST19
Regioni853 – 895Calmodulin-binding 2Add BLAST43

Domaini

Binding of calmodulin to binding site 1 is Ca2+ dependent, whereas binding of calmodulin to site 2 is Ca2+ independent.

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
InParanoidiP48994.
KOiK13803.
OMAiSQIGRCT.
OrthoDBiEOG091G029I.
PhylomeDBiP48994.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKKKLPTG VSSGVSHASS APKSVGGCCV PLGLPQPLLL EEKKFLLAVE
60 70 80 90 100
RGDMPNVRRI LQKALRHQHI NINCMDPLGR RALTLAIDNE NLEMVELLVV
110 120 130 140 150
MGVETKDALL HAINAEFVEA VELLLEHEEL IYKEGEPYSW QKVDINTAMF
160 170 180 190 200
APDITPLMLA AHKNNFEILR ILLDRGAAVP VPHDIRCGCE ECVRLTAEDS
210 220 230 240 250
LRHSLSRVNI YRALCSPSLI CLTSNDPIIT AFQLSWELRN LALTEQECKS
260 270 280 290 300
EYMDLRRQCQ KFAVDLLDQT RTSNELAIIL NYDPQMSSYE PGDRMSLTRL
310 320 330 340 350
VQAISYKQKK FVAHSNIQQL LSSIWYDGLP GFRRKSIVDK VICIAQVAVL
360 370 380 390 400
FPLYCLIYMC APNCRTGQLM RKPFMKFLIH ASSYLFFLFI LILVSQRADD
410 420 430 440 450
DFVRIFGTTR MKKELAEQEL RQRGQTPSKL ELIVVMYVIG FVWEEVQEIF
460 470 480 490 500
AVGMKSYLRN MWNFIDFLRN SLYVSVMCLR AFAYIQQATE IARDPQMAYI
510 520 530 540 550
PREKWHDFDP QLIAEGLFAA ANVFSALKLV HLFSINPHLG PLQISLGRMV
560 570 580 590 600
IDIVKFFFIY TLVLFAFACG LNQLLWYFAA LEKSKCYVLP GGEADWGSHG
610 620 630 640 650
DSCMKWRRFG NLFESSQSLF WASFGMVGLD DFELSGIKSY TRFWGLLMFG
660 670 680 690 700
SYSVINVIVL LNLLIAMMSN SYAMIDEHSD TEWKFARTKL WMSYFEDSAT
710 720 730 740 750
LPPPFNVLPS VKWVIRIFRK SSKTIDRQRS KKRKEQEQFS EYDNIMRSLV
760 770 780 790 800
WRYVAAMHRK FENNPVSEDD INEVKSEINT MRYEMLEIFE NSGMDVSSAN
810 820 830 840 850
KKERQPRPRR IKVWERRLMK GFQVAPVQNG CELDAFGNVN GQGEMQEIKV
860 870 880 890 900
ESIPSKPAKE TAKERFQRVA RTVLLQSTTH KWNVVLRAAK DSQIGRCTKN
910 920 930 940 950
ERKSLQNLGR AIEEAKRLIM LNPGCPSGRE SPIRIEFEDE KTSTLLELLN
960 970 980 990 1000
QISAEISDSE KPKIRPIWRP PLKTVPARAM AANNTRSLTA PELKISRKSS
1010 1020 1030 1040 1050
PAPTPTPTPG VSHTALSQFR NRELPLCPSK LIANSAPSAP TAPPKKSAPT
1060 1070 1080 1090 1100
APTPTYKPTT HAPFSVEGGN RENTRASDGV RSDNSNFDIH VVDLDEKGGH
1110 1120
LGRDNVSDIS SIASTSPQRP KHRN
Length:1,124
Mass (Da):127,750
Last modified:November 25, 2002 - v2
Checksum:iAF6323BA27626583
GO

Sequence cautioni

The sequence AAN71152 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228 – 229II → SS in AAA28979 (PubMed:1314616).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88185 mRNA. Translation: AAA28979.1.
AE013599 Genomic DNA. Translation: AAF58904.1.
AE013599 Genomic DNA. Translation: AAM68793.1.
AE013599 Genomic DNA. Translation: AAM68794.2.
BT001397 mRNA. Translation: AAN71152.1. Sequence problems.
BT099607 mRNA. Translation: ACU45760.1.
PIRiJH0588.
RefSeqiNP_476895.1. NM_057547.5.
NP_724822.1. NM_165694.3.
NP_724823.2. NM_165695.2.
UniGeneiDm.546.

Genome annotation databases

EnsemblMetazoaiFBtr0088471; FBpp0087555; FBgn0005614.
FBtr0088473; FBpp0087557; FBgn0005614.
FBtr0301662; FBpp0290876; FBgn0005614.
GeneIDi36003.
KEGGidme:Dmel_CG18345.
UCSCiCG18345-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88185 mRNA. Translation: AAA28979.1.
AE013599 Genomic DNA. Translation: AAF58904.1.
AE013599 Genomic DNA. Translation: AAM68793.1.
AE013599 Genomic DNA. Translation: AAM68794.2.
BT001397 mRNA. Translation: AAN71152.1. Sequence problems.
BT099607 mRNA. Translation: ACU45760.1.
PIRiJH0588.
RefSeqiNP_476895.1. NM_057547.5.
NP_724822.1. NM_165694.3.
NP_724823.2. NM_165695.2.
UniGeneiDm.546.

3D structure databases

ProteinModelPortaliP48994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61834. 4 interactors.
IntActiP48994. 2 interactors.
STRINGi7227.FBpp0290876.

Protein family/group databases

TCDBi1.A.4.1.8. the transient receptor potential ca(2+) channel (trp-cc) family.

Proteomic databases

PaxDbiP48994.
PRIDEiP48994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088471; FBpp0087555; FBgn0005614.
FBtr0088473; FBpp0087557; FBgn0005614.
FBtr0301662; FBpp0290876; FBgn0005614.
GeneIDi36003.
KEGGidme:Dmel_CG18345.
UCSCiCG18345-RA. d. melanogaster.

Organism-specific databases

CTDi36003.
FlyBaseiFBgn0005614. trpl.

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
InParanoidiP48994.
KOiK13803.
OMAiSQIGRCT.
OrthoDBiEOG091G029I.
PhylomeDBiP48994.

Enzyme and pathway databases

ReactomeiR-DME-3295583. TRP channels.
R-DME-5578775. Ion homeostasis.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiP48994.

Miscellaneous databases

ChiTaRSitrpl. fly.
GenomeRNAii36003.
PROiP48994.

Gene expression databases

BgeeiFBgn0005614.
GenevisibleiP48994. DM.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPL_DROME
AccessioniPrimary (citable) accession number: P48994
Secondary accession number(s): C6TPC1
, Q0E9E3, Q8IH62, Q8MKU9, Q9V5B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 25, 2002
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.