ID   ATOH1_MOUSE             Reviewed;         351 AA.
AC   P48985;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   27-MAR-2024, entry version 170.
DE   RecName: Full=Transcription factor Atoh1 {ECO:0000305};
DE   AltName: Full=Atonal bHLH transcription factor 1 {ECO:0000312|MGI:MGI:104654};
DE   AltName: Full=Helix-loop-helix protein mATH-1 {ECO:0000303|PubMed:7721778};
DE            Short=mATH1 {ECO:0000303|PubMed:7721778};
DE   AltName: Full=Protein atonal homolog 1 {ECO:0000312|MGI:MGI:104654};
GN   Name=Atoh1 {ECO:0000312|MGI:MGI:104654}; Synonyms=Ath1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RC   STRAIN=129/J;
RX   PubMed=7721778; DOI=10.1074/jbc.270.15.8730;
RA   Akazawa C., Ishibashi M., Shimizu C., Nakanishi S., Kageyama R.;
RT   "A mammalian helix-loop-helix factor structurally related to the product of
RT   Drosophila proneural gene atonal is a positive transcriptional regulator
RT   expressed in the developing nervous system.";
RL   J. Biol. Chem. 270:8730-8738(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=10648228; DOI=10.1242/dev.127.4.693;
RA   Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.;
RT   "Generation of neurons by transient expression of neural bHLH proteins in
RT   mammalian cells.";
RL   Development 127:693-702(2000).
RN   [4]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=17977745; DOI=10.1016/j.mcn.2007.09.006;
RA   Saul S.M., Brzezinski J.A. IV, Altschuler R.A., Shore S.E., Rudolph D.D.,
RA   Kabara L.L., Halsey K.E., Hufnagel R.B., Zhou J., Dolan D.F., Glaser T.;
RT   "Math5 expression and function in the central auditory system.";
RL   Mol. Cell. Neurosci. 37:153-169(2008).
CC   -!- FUNCTION: Transcriptional regulator. Activates E box-dependent
CC       transcription in collaboration with TCF3/E47, but the activity is
CC       completely antagonized by the negative regulator of neurogenesis HES1.
CC       Plays a role in the differentiation of subsets of neural cells by
CC       activating E box-dependent transcription.
CC       {ECO:0000269|PubMed:10648228}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein.
CC   -!- INTERACTION:
CC       P48985; Q91YV0: Tcf4; NbExp=2; IntAct=EBI-15659000, EBI-15658970;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC       ECO:0000269|PubMed:10648228}.
CC   -!- TISSUE SPECIFICITY: Developing nervous system, and in adult epithelial
CC       cells of the gastrointestinal tract. {ECO:0000269|PubMed:7721778}.
CC   -!- DEVELOPMENTAL STAGE: First detected in the cranial ganglions and the
CC       dorsal part of the central nervous system on 9.5 dpc (PubMed:7721778).
CC       From 10.5 dpc onward, prominently expressed in the dorsal part of the
CC       central nervous system but becomes restricted to the external granular
CC       layer of the cerebellum by 18 dpc and is undetectable in the adult
CC       nervous system (PubMed:7721778). Expressed in the cochlear nucleus at
CC       15.5 dpc (PubMed:17977745). {ECO:0000269|PubMed:17977745,
CC       ECO:0000269|PubMed:7721778}.
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DR   EMBL; D43694; BAA07791.1; -; Genomic_DNA.
DR   EMBL; BC010820; AAH10820.1; -; mRNA.
DR   EMBL; BC051256; AAH51256.1; -; mRNA.
DR   CCDS; CCDS20203.1; -.
DR   PIR; A56387; A56387.
DR   RefSeq; NP_031526.1; NM_007500.4.
DR   AlphaFoldDB; P48985; -.
DR   SMR; P48985; -.
DR   BioGRID; 198237; 1.
DR   DIP; DIP-46434N; -.
DR   IntAct; P48985; 3.
DR   STRING; 10090.ENSMUSP00000098903; -.
DR   iPTMnet; P48985; -.
DR   PhosphoSitePlus; P48985; -.
DR   PaxDb; 10090-ENSMUSP00000098903; -.
DR   ProteomicsDB; 265154; -.
DR   Antibodypedia; 25709; 605 antibodies from 32 providers.
DR   DNASU; 11921; -.
DR   Ensembl; ENSMUST00000101351.6; ENSMUSP00000098903.5; ENSMUSG00000073043.6.
DR   GeneID; 11921; -.
DR   KEGG; mmu:11921; -.
DR   UCSC; uc009ceb.2; mouse.
DR   AGR; MGI:104654; -.
DR   CTD; 474; -.
DR   MGI; MGI:104654; Atoh1.
DR   VEuPathDB; HostDB:ENSMUSG00000073043; -.
DR   eggNOG; KOG4395; Eukaryota.
DR   GeneTree; ENSGT00940000160064; -.
DR   HOGENOM; CLU_083039_0_0_1; -.
DR   InParanoid; P48985; -.
DR   OMA; CKNDHHH; -.
DR   OrthoDB; 5406645at2759; -.
DR   PhylomeDB; P48985; -.
DR   TreeFam; TF315153; -.
DR   BioGRID-ORCS; 11921; 0 hits in 78 CRISPR screens.
DR   PRO; PR:P48985; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; P48985; Protein.
DR   Bgee; ENSMUSG00000073043; Expressed in cerebellum external granule cell layer and 77 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0031490; F:chromatin DNA binding; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IDA:MGI.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0042668; P:auditory receptor cell fate determination; IDA:MGI.
DR   GO; GO:0042667; P:auditory receptor cell fate specification; IMP:MGI.
DR   GO; GO:0061564; P:axon development; IBA:GO_Central.
DR   GO; GO:0007411; P:axon guidance; IMP:MGI.
DR   GO; GO:0007420; P:brain development; IMP:MGI.
DR   GO; GO:0030154; P:cell differentiation; IMP:MGI.
DR   GO; GO:0021987; P:cerebral cortex development; IMP:MGI.
DR   GO; GO:1904019; P:epithelial cell apoptotic process; IMP:MGI.
DR   GO; GO:0042491; P:inner ear auditory receptor cell differentiation; IDA:MGI.
DR   GO; GO:0048839; P:inner ear development; IMP:MGI.
DR   GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI.
DR   GO; GO:1904036; P:negative regulation of epithelial cell apoptotic process; IMP:MGI.
DR   GO; GO:0014014; P:negative regulation of gliogenesis; IGI:UniProtKB.
DR   GO; GO:0097402; P:neuroblast migration; IMP:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR   GO; GO:0048663; P:neuron fate commitment; IBA:GO_Central.
DR   GO; GO:0001764; P:neuron migration; IMP:MGI.
DR   GO; GO:0007219; P:Notch signaling pathway; IDA:MGI.
DR   GO; GO:0045609; P:positive regulation of inner ear auditory receptor cell differentiation; IDA:MGI.
DR   GO; GO:2000982; P:positive regulation of inner ear receptor cell differentiation; IMP:UniProtKB.
DR   GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0045664; P:regulation of neuron differentiation; IDA:MGI.
DR   GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IMP:MGI.
DR   CDD; cd19713; bHLH_TS_ATOH1; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR032661; ATOH1_bHLH.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   PANTHER; PTHR19290; BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED; 1.
DR   PANTHER; PTHR19290:SF82; TRANSCRIPTION FACTOR ATOH1; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   Genevisible; P48985; MM.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; Differentiation; DNA-binding;
KW   Neurogenesis; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..351
FT                   /note="Transcription factor Atoh1"
FT                   /id="PRO_0000127140"
FT   DOMAIN          156..208
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          16..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          89..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          244..278
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          308..351
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        326..351
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   351 AA;  37854 MW;  596E03DF23C3BF96 CRC64;
     MSRLLHAEEW AEVKELGDHH RHPQPHHVPP LTPQPPATLQ ARDLPVYPAE LSLLDSTDPR
     AWLTPTLQGL CTARAAQYLL HSPELGASEA AAPRDEADSQ GELVRRSGCG GLSKSPGPVK
     VREQLCKLKG GVVVDELGCS RQRAPSSKQV NGVQKQRRLA ANARERRRMH GLNHAFDQLR
     NVIPSFNNDK KLSKYETLQM AQIYINALSE LLQTPNVGEQ PPPPTASCKN DHHHLRTASS
     YEGGAGASAV AGAQPAPGGG PRPTPPGPCR TRFSGPASSG GYSVQLDALH FPAFEDRALT
     AMMAQKDLSP SLPGGILQPV QEDNSKTSPR SHRSDGEFSP HSHYSDSDEA S
//