Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P48982 (BGAL_XANMN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Lactase
EC=3.2.1.23
Gene names
Name:bga
OrganismXanthomonas manihotis
Taxonomic identifier43353 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Preferentially hydrolyzes beta(1->3) galactosyl linkages over beta(1->4) linkages.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.5. Ref.1

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.1
Chain22 – 598577Beta-galactosidase
PRO_0000012198

Sites

Active site1841Proton donor Potential
Active site2601Nucleophile Potential

Sequences

Sequence LengthMass (Da)Tools
P48982 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: DB4C3F05E01435BF

FASTA59866,086
        10         20         30         40         50         60 
MLRTTLAPLV LALALALPAA AATPESWPTF GTQGTQFVRD GKPYQLLSGA IHFQRIPRAY 

        70         80         90        100        110        120 
WKDRLQKARA LGLNTVETYV FWNLVEPQQG QFDFSGNNDV AAFVKEAAAQ GLNVILRPGP 

       130        140        150        160        170        180 
YACAEWEAGG YPAWLFGKGN IRVRSRDPRF LAASQAYLDA LAKQVQPLLN HNGGPIIAVQ 

       190        200        210        220        230        240 
VENEYGSYAD DHAYMADNRA MYVKAGFDKA LLFTSDGADM LANGTLPDTL AVVNFAPGEA 

       250        260        270        280        290        300 
KSAFDKLIKF RPDQPRMVGE YWAGWFDHWG KPHAATDARQ QAEEFEWILR QGHSANLYMF 

       310        320        330        340        350        360 
IGGTSFGFMN GANFQNNPSD HYAPQTTSYD YDAILDEAGH PTPKFALMRD AIARVTGVQP 

       370        380        390        400        410        420 
PALPAPITTT TLPATPLRES ASLWDNLPTP IAIDTPQPME QFGQDYGYIL YRTTITGPRK 

       430        440        450        460        470        480 
GPLYLGDVRD VARVYVDQRP VGSVERRLQQ VSLEVEIPAG QHTLDVLVEN SGRINYGTRM 

       490        500        510        520        530        540 
ADGRAGLVDP VLLDSQQLTG WQAFPLPMRT PDSIRGWTGK AVQGPAFHRG TLRIGTPTDT 

       550        560        570        580        590 
YLDMRAFGKG FAWANGVNLG RHWNIGPQTA LYLRPSSARV TTRWWSSTWT MLHPSVRG 

« Hide

References

[1]"A novel beta-galactosidase gene isolated from the bacterium Xanthomonas manihotis exhibits strong homology to several eukaryotic beta-galactosidases."
Taron C.H., Benner J.S., Hornstra L.J., Guthrie E.P.
Glycobiology 5:603-610(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-38, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: 7AS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35444 Genomic DNA. Translation: AAC41485.1.
PIRA57249.

3D structure databases

ProteinModelPortalP48982.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFPIRSF006336. B-gal. 1 hit.
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_XANMN
AccessionPrimary (citable) accession number: P48982
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries