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Protein

Beta-galactosidase

Gene

bga

Organism
Xanthomonas manihotis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially hydrolyzes beta(1->3) galactosyl linkages over beta(1->4) linkages.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

pH dependencei

Optimum pH is 4.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei184 – 1841Proton donorSequence Analysis
Active sitei260 – 2601NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
Gene namesi
Name:bga
OrganismiXanthomonas manihotis
Taxonomic identifieri43353 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 598577Beta-galactosidasePRO_0000012198Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP48982.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48982-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTTLAPLV LALALALPAA AATPESWPTF GTQGTQFVRD GKPYQLLSGA
60 70 80 90 100
IHFQRIPRAY WKDRLQKARA LGLNTVETYV FWNLVEPQQG QFDFSGNNDV
110 120 130 140 150
AAFVKEAAAQ GLNVILRPGP YACAEWEAGG YPAWLFGKGN IRVRSRDPRF
160 170 180 190 200
LAASQAYLDA LAKQVQPLLN HNGGPIIAVQ VENEYGSYAD DHAYMADNRA
210 220 230 240 250
MYVKAGFDKA LLFTSDGADM LANGTLPDTL AVVNFAPGEA KSAFDKLIKF
260 270 280 290 300
RPDQPRMVGE YWAGWFDHWG KPHAATDARQ QAEEFEWILR QGHSANLYMF
310 320 330 340 350
IGGTSFGFMN GANFQNNPSD HYAPQTTSYD YDAILDEAGH PTPKFALMRD
360 370 380 390 400
AIARVTGVQP PALPAPITTT TLPATPLRES ASLWDNLPTP IAIDTPQPME
410 420 430 440 450
QFGQDYGYIL YRTTITGPRK GPLYLGDVRD VARVYVDQRP VGSVERRLQQ
460 470 480 490 500
VSLEVEIPAG QHTLDVLVEN SGRINYGTRM ADGRAGLVDP VLLDSQQLTG
510 520 530 540 550
WQAFPLPMRT PDSIRGWTGK AVQGPAFHRG TLRIGTPTDT YLDMRAFGKG
560 570 580 590
FAWANGVNLG RHWNIGPQTA LYLRPSSARV TTRWWSSTWT MLHPSVRG
Length:598
Mass (Da):66,086
Last modified:February 1, 1996 - v1
Checksum:iDB4C3F05E01435BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35444 Genomic DNA. Translation: AAC41485.1.
PIRiA57249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35444 Genomic DNA. Translation: AAC41485.1.
PIRiA57249.

3D structure databases

ProteinModelPortaliP48982.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel beta-galactosidase gene isolated from the bacterium Xanthomonas manihotis exhibits strong homology to several eukaryotic beta-galactosidases."
    Taron C.H., Benner J.S., Hornstra L.J., Guthrie E.P.
    Glycobiology 5:603-610(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-38, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: 7AS1.

Entry informationi

Entry nameiBGAL_XANMN
AccessioniPrimary (citable) accession number: P48982
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.