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P48981 (BGAL_MALDO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

EC=3.2.1.23
Alternative name(s):
Acid beta-galactosidase
Short name=Lactase
Exo-(1-->4)-beta-D-galactanase
OrganismMalus domestica (Apple) (Pyrus malus)
Taxonomic identifier3750 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeMaleaeMalus

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell wall degradation. Degrades polysaccharides containing beta-(1-->4)-linked galactans, acting as an exo-(1-->4)-beta-D-galactanase.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 731708Beta-galactosidase
PRO_0000012197

Sites

Active site1821Proton donor Potential
Active site2511Nucleophile Potential

Amino acid modifications

Glycosylation4591N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P48981 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: FAB65D24A0D30BD4

FASTA73180,996
        10         20         30         40         50         60 
MGVGIQTMWS ILLLFSCIFS AASASVSYDH KAIIINGQKR ILISGSIHYP RSTPEMWPDL 

        70         80         90        100        110        120 
IQKAKDGGLD VIQTYVFWNG HEPSPGNYYF EERYDLVKFI KLVQQEGLFV NLRIGPYVCA 

       130        140        150        160        170        180 
EWNFGGFPVW LKYVPGIAFR TDNEPFKAAM QKFTEKIVSM MKAEKLFQTQ GGPIILSQIE 

       190        200        210        220        230        240 
NEFGPVEWEI GAPGKAYTKW AAQMAVGLDT GVPWIMCKQE DAPDPVIDTC NGFYCENFKP 

       250        260        270        280        290        300 
NKDYKPKMWT EVWTGWYTEF GGAVPTRPAE DVAFSVARFI QSGGSFLNYY MYHGGTNFGR 

       310        320        330        340        350        360 
TAGGPFMATS YDYDAPLDEY GLPREPKWGH LRDLHKAIKS CESALVSVDP SVTKLGSNQE 

       370        380        390        400        410        420 
AHVFKSESDC AAFLANYDAK YSVKVSFGGG QYDLPPWSIS ILPDCKTEVY NTAKVGSQSS 

       430        440        450        460        470        480 
QVQMTPVHSG FPWQSFIEET TSSDETDTTT LDGLYEQINI TRDTTDYLWY MTDITIGSDE 

       490        500        510        520        530        540 
AFLKNGKSPL LTIFSAGHAL NVFINGQLSG TVYGSLENPK LSFSQNVNLR SGINKLALLS 

       550        560        570        580        590        600 
ISVGLPNVGT HFETWNAGVL GPITLKGLNS GTWDMSGWKW TYKTGLKGEA LGLHTVTGSS 

       610        620        630        640        650        660 
SVEWVEGPSM AEKQPLTWYK ATFNAPPGDA PLALDMGSMG KGQIWINGQS VGRHWPGYIA 

       670        680        690        700        710        720 
RGSCGDCSYA GTYDDKKCRT HCGEPSQRWY HIPRSWLTPT GNLLVVFEEW GGDPSRISLV 

       730 
ERGTALDAKK L 

« Hide

References

[1]"Apple beta-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone."
Ross G.S., Wegrzyn T., Macrae E.A., Redgwell R.J.
Plant Physiol. 106:521-528(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Granny Smith.
Tissue: Fruit cortical tissue.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29451 mRNA. Translation: AAA62324.1.
PIRT17002.

3D structure databases

ProteinModelPortalP48981.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_MALDO
AccessionPrimary (citable) accession number: P48981
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries