Reviewed, UniProtKB/Swiss-Prot P48981 (BGAL_MALDO)

Names and origin

Protein names	Recommended name:     Beta-galactosidase     EC=3.2.1.23 Alternative name(s):     Acid beta-galactosidase       Short name=Lactase     Exo-(1-->4)-beta-D-galactanase
Organism	Malus domestica (Apple) (Malus sylvestris)
Taxonomic identifier	3750 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cell wall degradation. Degrades polysaccharides containing beta-(1-->4)-linked galactans, acting as an exo-(1-->4)-beta-D-galactanase.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 731	708	Beta-galactosidase		PRO_0000012197
Sites
	Active site	182	1	Proton donor Potential
	Active site	251	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	459	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48981-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: FAB65D24A0D30BD4	FASTA	731	80,996
	10 20 30 40 50 60 MGVGIQTMWS ILLLFSCIFS AASASVSYDH KAIIINGQKR ILISGSIHYP RSTPEMWPDL 70 80 90 100 110 120 IQKAKDGGLD VIQTYVFWNG HEPSPGNYYF EERYDLVKFI KLVQQEGLFV NLRIGPYVCA 130 140 150 160 170 180 EWNFGGFPVW LKYVPGIAFR TDNEPFKAAM QKFTEKIVSM MKAEKLFQTQ GGPIILSQIE 190 200 210 220 230 240 NEFGPVEWEI GAPGKAYTKW AAQMAVGLDT GVPWIMCKQE DAPDPVIDTC NGFYCENFKP 250 260 270 280 290 300 NKDYKPKMWT EVWTGWYTEF GGAVPTRPAE DVAFSVARFI QSGGSFLNYY MYHGGTNFGR 310 320 330 340 350 360 TAGGPFMATS YDYDAPLDEY GLPREPKWGH LRDLHKAIKS CESALVSVDP SVTKLGSNQE 370 380 390 400 410 420 AHVFKSESDC AAFLANYDAK YSVKVSFGGG QYDLPPWSIS ILPDCKTEVY NTAKVGSQSS 430 440 450 460 470 480 QVQMTPVHSG FPWQSFIEET TSSDETDTTT LDGLYEQINI TRDTTDYLWY MTDITIGSDE 490 500 510 520 530 540 AFLKNGKSPL LTIFSAGHAL NVFINGQLSG TVYGSLENPK LSFSQNVNLR SGINKLALLS 550 560 570 580 590 600 ISVGLPNVGT HFETWNAGVL GPITLKGLNS GTWDMSGWKW TYKTGLKGEA LGLHTVTGSS 610 620 630 640 650 660 SVEWVEGPSM AEKQPLTWYK ATFNAPPGDA PLALDMGSMG KGQIWINGQS VGRHWPGYIA 670 680 690 700 710 720 RGSCGDCSYA GTYDDKKCRT HCGEPSQRWY HIPRSWLTPT GNLLVVFEEW GGDPSRISLV 730 ERGTALDAKK L

References

[1]

"Apple beta-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone." Ross G.S., Wegrzyn T., Macrae E.A., Redgwell R.J. Plant Physiol. 106:521-528(1994) [PubMed: 7991682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Granny Smith. Tissue: Fruit cortical tissue.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L29451 mRNA. Translation: AAA62324.1.
PIR	T17002.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Enzyme and pathway databases
BRENDA	3.2.1.23. 66979.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_MALDO

Accession

Primary (citable) accession number: P48981

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)