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P48981

- BGAL_MALDO

UniProt

P48981 - BGAL_MALDO

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Protein

Beta-galactosidase

Gene
N/A
Organism
Malus domestica (Apple) (Pyrus malus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in cell wall degradation. Degrades polysaccharides containing beta-(1-->4)-linked galactans, acting as an exo-(1-->4)-beta-D-galactanase.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei182 – 1821Proton donorSequence Analysis
Active sitei251 – 2511NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Exo-(1-->4)-beta-D-galactanase
OrganismiMalus domestica (Apple) (Pyrus malus)
Taxonomic identifieri3750 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeMaleaeMalus

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 731708Beta-galactosidasePRO_0000012197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi459 – 4591N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP48981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48981-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVGIQTMWS ILLLFSCIFS AASASVSYDH KAIIINGQKR ILISGSIHYP
60 70 80 90 100
RSTPEMWPDL IQKAKDGGLD VIQTYVFWNG HEPSPGNYYF EERYDLVKFI
110 120 130 140 150
KLVQQEGLFV NLRIGPYVCA EWNFGGFPVW LKYVPGIAFR TDNEPFKAAM
160 170 180 190 200
QKFTEKIVSM MKAEKLFQTQ GGPIILSQIE NEFGPVEWEI GAPGKAYTKW
210 220 230 240 250
AAQMAVGLDT GVPWIMCKQE DAPDPVIDTC NGFYCENFKP NKDYKPKMWT
260 270 280 290 300
EVWTGWYTEF GGAVPTRPAE DVAFSVARFI QSGGSFLNYY MYHGGTNFGR
310 320 330 340 350
TAGGPFMATS YDYDAPLDEY GLPREPKWGH LRDLHKAIKS CESALVSVDP
360 370 380 390 400
SVTKLGSNQE AHVFKSESDC AAFLANYDAK YSVKVSFGGG QYDLPPWSIS
410 420 430 440 450
ILPDCKTEVY NTAKVGSQSS QVQMTPVHSG FPWQSFIEET TSSDETDTTT
460 470 480 490 500
LDGLYEQINI TRDTTDYLWY MTDITIGSDE AFLKNGKSPL LTIFSAGHAL
510 520 530 540 550
NVFINGQLSG TVYGSLENPK LSFSQNVNLR SGINKLALLS ISVGLPNVGT
560 570 580 590 600
HFETWNAGVL GPITLKGLNS GTWDMSGWKW TYKTGLKGEA LGLHTVTGSS
610 620 630 640 650
SVEWVEGPSM AEKQPLTWYK ATFNAPPGDA PLALDMGSMG KGQIWINGQS
660 670 680 690 700
VGRHWPGYIA RGSCGDCSYA GTYDDKKCRT HCGEPSQRWY HIPRSWLTPT
710 720 730
GNLLVVFEEW GGDPSRISLV ERGTALDAKK L
Length:731
Mass (Da):80,996
Last modified:February 1, 1996 - v1
Checksum:iFAB65D24A0D30BD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29451 mRNA. Translation: AAA62324.1.
PIRiT17002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29451 mRNA. Translation: AAA62324.1 .
PIRi T17002.

3D structure databases

ProteinModelPortali P48981.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Apple beta-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone."
    Ross G.S., Wegrzyn T., Macrae E.A., Redgwell R.J.
    Plant Physiol. 106:521-528(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Granny Smith.
    Tissue: Fruit cortical tissue.

Entry informationi

Entry nameiBGAL_MALDO
AccessioniPrimary (citable) accession number: P48981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3