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P48980 (BGAL_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

EC=3.2.1.23
Alternative name(s):
Acid beta-galactosidase
Short name=Lactase
Exo-(1-->4)-beta-D-galactanase
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell wall degradation. Degrades polysaccharides containing beta-(1-->4)-linked galactans, acting as an exo-(1-->4)-beta-D-galactanase.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.5.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentplant-type cell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 835813Beta-galactosidase
PRO_0000012195

Regions

Domain749 – 83587SUEL-type lectin

Sites

Active site1801Proton donor Potential
Active site2491Nucleophile Potential

Sequences

Sequence LengthMass (Da)Tools
P48980 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 94C9685F95C4A646

FASTA83593,336
        10         20         30         40         50         60 
MGFWMAMLLM LLLCLWVSCG IASVSYDHKA IIVNGQRKIL ISGSIHYPRS TPEMWPDLIQ 

        70         80         90        100        110        120 
KAKEGGVDVI QTYVFWNGHE PEEGKYYFEE RYDLVKFIKV VQEAGLYVHL RIGPYACAEW 

       130        140        150        160        170        180 
NFGGFPVWLK YVPGISFRTN NEPFKAAMQK FTTKIVDMMK AEKLYETQGG PIILSQIENE 

       190        200        210        220        230        240 
YGPMEWELGE PGKVYSEWAA KMAVDLGTGV PWIMCKQDDV PDPIINTCNG FYCDYFTPNK 

       250        260        270        280        290        300 
ANKPKMWTEA WTAWFTEFGG PVPYRPAEDM AFAVARFIQT GGSFINYYMY HGGTNFGRTS 

       310        320        330        340        350        360 
GGPFIATSYD YDAPLDEFGS LRQPKWGHLK DLHRAIKLCE PALVSVDPTV TSLGNYQEAR 

       370        380        390        400        410        420 
VFKSESGACA AFLANYNQHS FAKVAFGNMH YNLPPWSISI LPDCKNTVYN TARVGAQSAQ 

       430        440        450        460        470        480 
MKMTPVSRGF SWESFNEDAA SHEDDTFTVV GLLEQINITR DVSDYLWYMT DIEIDPTEGF 

       490        500        510        520        530        540 
LNSGNWPWLT VFSAGHALHV FVNGQLAGTV YGSLENPKLT FSNGINLRAG VNKISLLSIA 

       550        560        570        580        590        600 
VGLPNVGPHF ETWNAGVLGP VSLNGLNEGT RDLTWQKWFY KVGLKGEALS LHSLSGSPSV 

       610        620        630        640        650        660 
EWVEGSLVAQ KQPLSWYKTT FNAPDGNEPL ALDMNTMGKG QVWINGQSLG RHWPAYKSSG 

       670        680        690        700        710        720 
SCSVCNYTGW FDEKKCLTNC GEGSQRWYHV PRSWLYPTGN LLVVFEEWGG DPYGITLVKR 

       730        740        750        760        770        780 
EIGSVCADIY EWQPQLLNWQ RLVSGKFDRP LRPKAHLKCA PGQKISSIKF ASFGTPEGVC 

       790        800        810        820        830 
GNFQQGSCHA PRSYDAFKKN CVGKESCSVQ VTPENFGGDP CRNVLKKLSV EAICS 

« Hide

References

[1]"Tomato exo-(1-->4)-beta-D-galactanase. Isolation, changes during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone."
Carey A.T., Holt K., Picard S., Wilde R., Tucker G.A., Bird C.R., Schuch W., Seymour G.B.
Plant Physiol. 108:1099-1107(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Ailsa Craig.
Tissue: Pericarp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83854 mRNA. Translation: CAA58734.1.
PIRT06590.
RefSeqNP_001234465.1. NM_001247536.1.
UniGeneLes.3979.

3D structure databases

ProteinModelPortalP48980.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc12g044880.1.1; Solyc12g044880.1.1; Solyc12g044880.1.
GeneID544167.
KEGGsly:544167.

Phylogenomic databases

OMAINITRDV.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_SOLLC
AccessionPrimary (citable) accession number: P48980
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries