P48979 (PGLR_PRUPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polygalacturonase Short name=PG EC=3.2.1.15 Alternative name(s): Pectinase
Organism	Prunus persica (Peach) (Amygdalus persica)
Taxonomic identifier	3760 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Maloideae › Amygdaleae › Prunus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in concert with the pectinesterase, in the ripening process. Is involved in cell wall metabolism, specifically in polyuronide degradation.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 393	368	Polygalacturonase		PRO_0000024812
Sites
	Active site	219	1	Proton donor By similarity
	Active site	242	1	By similarity
Amino acid modifications
	Glycosylation	260	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48979 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 53DCC26944D22BF9	FASTA	393	41,472
	10 20 30 40 50 60 MANRRSLFSL SLIFVFMINS AIASPLTYNV ASLGAKADGK TDSTKAFLSA WAKACASMNP 70 80 90 100 110 120 GVIYVPAGTF FLRDVVFSGP CKNNAITFRI AGTLVAPSDY RVIGNAANWI FFHHVNGVTI 130 140 150 160 170 180 SGGILDGQGT ALWACKACHG ESCPSGATTL GFSDSNNIVV SGLASLNSQM FHIVINDFQN 190 200 210 220 230 240 VQMQGVRVSR SGNSPNTDGI HVQMSSGVTI LNSKIATGDD CVSIGPGTSN LWIEGVACGP 250 260 270 280 290 300 GHGISIGSLG KEQEEAGVQN VTVKTVTFSG TQNGLRIKSW GRPSTGFARN ILFQHATMVN 310 320 330 340 350 360 VENPIVIDQH YCPDNKGCPG QVSGVQISDV TYEDIHGTSA TEVAVKFDCS PKHPCREIKL 370 380 390 EDVKLTYKNQ AAESSCSHAD GTTEGVVQPT SCL

References

[1]	"Peach (Prunus persica) endopolygalacturonase cDNA isolation and mRNA analysis in melting and nonmelting peach cultivars." Lester D.R., Speiers J., Orr G., Brady C.J. Plant Physiol. 105:225-231(1994) [PubMed: 8029352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Flavorcrest.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76735 mRNA. Translation: CAA54150.1.
PIR	S40123.
3D structure databases
ProteinModelPortal	P48979.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14862.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PRUPE

Accession

Primary (citable) accession number: P48979

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	October 19, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program