Reviewed, UniProtKB/Swiss-Prot P48978 (PGLR_MALDO)

Names and origin

Protein names	Recommended name:     Polygalacturonase       Short name=PG     EC=3.2.1.15 Alternative name(s):     Pectinase
Organism	Malus domestica (Apple) (Malus sylvestris)
Taxonomic identifier	3750 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in concert with the pectinesterase, in the ripening process. Is involved in cell wall metabolism, specifically in polyuronide degradation.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 460	434	Polygalacturonase		PRO_0000024809
Sites
	Active site	292	1	Proton donor By similarity
	Active site	315	1	By similarity
Amino acid modifications
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48978-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 13E99A7A049F4FCE	FASTA	460	49,404
	10 20 30 40 50 60 MALKTQLLWS FVVVFVVSFS TTSCSGSSFQ EVNALHSYVD HVDDKESGYN SRAYPSYTDT 70 80 90 100 110 120 IEGLKVMELI RPRTQLFSSR KLNTITGGIA TSSAPAKTIS VDDFGAKGNG ADDTQAFVKA 130 140 150 160 170 180 WKAACSSSGA MVLVVPQKNY LVRPIEFSGP CKSQLTLQIY GTIEASEDRS IYKDIDHWLI 190 200 210 220 230 240 FDNVQNLLVV GPGTINGNGN IWWKNSCKIK PQPPCGTYAP TAVTFNRCNN LVVKNLNIQD 250 260 270 280 290 300 AQQIHVIFQN CINVQASCLT VTAPEDSPNT DGIHVTNTQN ITISSSVIGT GDDCISIVSG 310 320 330 340 350 360 SQRVQATDIT CGPGHGISIG SLGEDGSEDH VSGVFVNGAK LSGTSNGLRI KTWKGGSGSA 370 380 390 400 410 420 TNIVFQNVQM NDVTNPIIID QNYCDHKTKD CKQQKSAVQV KNVLYQNIRG TSASGDAITL 430 440 450 460 NCSQSVPCQG IVLQSVQLQN GRAECNNVQP AYKGVVSPRC

References

[1]	"A cDNA clone for endopolygalacturonase from apple." Atkinson R.G. Plant Physiol. 105:1437-1438(1994) [PubMed: 7972500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Golden Delicious.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27743 mRNA. Translation: AAA74452.1.
PIR	T17011.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 66979.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_MALDO

Accession

Primary (citable) accession number: P48978

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)