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P48968

- MPIP3_MESAU

UniProt

P48968 - MPIP3_MESAU

Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei322 – 3221By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 3 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25C
    Gene namesi
    Name:CDC25C
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 420419M-phase inducer phosphatase 3PRO_0000198649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei38 – 381PhosphoserineBy similarity
    Modified residuei54 – 541PhosphoserineBy similarity
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei61 – 611PhosphoserineBy similarity
    Modified residuei64 – 641Phosphothreonine; by CDK1By similarity
    Modified residuei126 – 1261PhosphoserineBy similarity
    Modified residuei127 – 1271Phosphothreonine; by CDK1By similarity
    Modified residuei198 – 1981Phosphoserine; by CDK1By similarity
    Modified residuei219 – 2191Phosphoserine; by PLK3By similarity
    Modified residuei226 – 2261Phosphoserine; by PLK3By similarity
    Modified residuei418 – 4181PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-219 promotes nuclear translocation. Ser-226 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-126 and/or Thr-127, interacts with PLK1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP48968.
    SMRiP48968. Positions 232-386.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini266 – 373108RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG052501.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48968-1 [UniParc]FASTAAdd to Basket

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    MSTGPFPSSR REESSVSAPS FRFSQRKMLN LLLERNTSFT QDFPRSPGDK    50
    LLDSTNLSIL SGGTPKRCLD LSNLSNGEMS ASPLITSADF DDTGSLDSSG 100
    PQDVQLTEKN HHQDPMKGIP VQLLCSTPNA LDHSHRKKDA VRGLSANKEN 150
    INTNLKTLQW ESPRIPRFQN TPGDPLASPL PLLGNGVSMD TEVRSLGSPI 200
    TAVPKLSKNL NLEDQEEISE EPMEFSLEDH DTKECVLPTV SGKHQDLKYI 250
    TPDTVAALLS GKFQGLIEKF YIIDCRYPYE YLGGHILGAI NLCSQKELHE 300
    FFLKKPIVPL DIQKRVIIVF LCEFSSERGP RMCRSLRRKD RALNQYPALY 350
    YPELYILKGG YRDFFPEYTE LCEPQGYCPM HHQDHQAELL MWRNQSKAQE 400
    GERQLSEQIA LLMKKGVSLP 420
    Length:420
    Mass (Da):47,327
    Last modified:February 1, 1996 - v1
    Checksum:iD7564B0AF2124783
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10878 mRNA. Translation: BAA01646.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10878 mRNA. Translation: BAA01646.1 .

    3D structure databases

    ProteinModelPortali P48968.
    SMRi P48968. Positions 232-386.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG052501.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm."
      Seki T., Yamashita K., Nishitani H., Takagi T., Russell P., Nishimoto T.
      Mol. Biol. Cell 3:1373-1388(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiMPIP3_MESAU
    AccessioniPrimary (citable) accession number: P48968
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3