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Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei322By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:CDC25C
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeMesocricetus
Proteomesi
  • UP000189706 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001986492 – 420M-phase inducer phosphatase 3Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei38PhosphoserineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei58PhosphoserineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei64Phosphothreonine; by CDK1By similarity1
Modified residuei126PhosphoserineBy similarity1
Modified residuei127PhosphothreonineBy similarity1
Modified residuei198Phosphoserine; by CDK1By similarity1
Modified residuei219Phosphoserine; by PLK3By similarity1
Modified residuei226Phosphoserine; by PLK3By similarity1
Modified residuei418PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-219 promotes nuclear translocation. Ser-226 is a minor phosphorylation site (By similarity). Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-126 and/or Thr-127, interacts with PLK1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP48968.
SMRiP48968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini266 – 373RhodanesePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

HOVERGENiHBG052501.
OrthoDBiEOG091G0H0D.

Family and domain databases

CDDicd01530. Cdc25. 1 hit.
Gene3Di3.40.250.10. 1 hit.
InterProiView protein in InterPro
IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
IPR036873. Rhodanese-like_dom_sf.
PfamiView protein in Pfam
PF00581. Rhodanese. 1 hit.
PRINTSiPR00716. MPIPHPHTASE.
SMARTiView protein in SMART
SM00450. RHOD. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGPFPSSR REESSVSAPS FRFSQRKMLN LLLERNTSFT QDFPRSPGDK
60 70 80 90 100
LLDSTNLSIL SGGTPKRCLD LSNLSNGEMS ASPLITSADF DDTGSLDSSG
110 120 130 140 150
PQDVQLTEKN HHQDPMKGIP VQLLCSTPNA LDHSHRKKDA VRGLSANKEN
160 170 180 190 200
INTNLKTLQW ESPRIPRFQN TPGDPLASPL PLLGNGVSMD TEVRSLGSPI
210 220 230 240 250
TAVPKLSKNL NLEDQEEISE EPMEFSLEDH DTKECVLPTV SGKHQDLKYI
260 270 280 290 300
TPDTVAALLS GKFQGLIEKF YIIDCRYPYE YLGGHILGAI NLCSQKELHE
310 320 330 340 350
FFLKKPIVPL DIQKRVIIVF LCEFSSERGP RMCRSLRRKD RALNQYPALY
360 370 380 390 400
YPELYILKGG YRDFFPEYTE LCEPQGYCPM HHQDHQAELL MWRNQSKAQE
410 420
GERQLSEQIA LLMKKGVSLP
Length:420
Mass (Da):47,327
Last modified:February 1, 1996 - v1
Checksum:iD7564B0AF2124783
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10878 mRNA. Translation: BAA01646.1.
RefSeqiXP_012979162.1. XM_013123708.1.

Genome annotation databases

GeneIDi101828859.

Similar proteinsi

Entry informationi

Entry nameiMPIP3_MESAU
AccessioniPrimary (citable) accession number: P48968
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families