P48967 (MPIP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase 3
EC=3.1.3.48

Alternative name(s):
Dual specificity phosphatase Cdc25C

Gene names

Name:	Cdc25c
Synonyms:	Cdc25m1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity. May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with MAPK14 and 14-3-3 proteins By similarity. When phosphorylated at Ser-128 and/or Thr-129, interacts with PLK1 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Spleen and thymus.
Post-translational modification	Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity By similarity. Phosphorylated by CHEK1 and MAPKAPK2. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase activity. Phosphorylation by PLK3 at Ser-213 promotes nuclear translocation. Ser-220 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from direct assay PubMed 12937170. Source: MGI nucleus Inferred from direct assay PubMed 12937170. Source: MGI
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	M-phase inducer phosphatase 3		PRO_0000198648
Regions
	Domain	294 – 401	108	Rhodanese
Sites
	Active site	350	1	By similarity
Amino acid modifications
	Modified residue	38	1	Phosphoserine By similarity
	Modified residue	56	1	Phosphoserine By similarity
	Modified residue	60	1	Phosphoserine By similarity
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	66	1	Phosphothreonine; by CDK1 By similarity
	Modified residue	128	1	Phosphoserine By similarity
	Modified residue	129	1	Phosphothreonine; by CDK1 By similarity
	Modified residue	192	1	Phosphoserine; by CDK1 By similarity
	Modified residue	213	1	Phosphoserine; by PLK3 By similarity
	Modified residue	220	1	Phosphoserine; by PLK3 By similarity
	Modified residue	445	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	153 – 165	13	INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409. Ref.2
	Sequence conflict	336	1	V → L in AAA74912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48967 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 513E51BAB3D1A39E	FASTA	447	50,046
	10 20 30 40 50 60 MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK DKLHDSANLS 70 80 90 100 110 120 ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS SGPLDRQLTG KDFHQDLMKG 130 140 150 160 170 180 IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK ENINTSLKAL EWEAPRTPRF RKMPGGPLTS 190 200 210 220 230 240 PLCELEMKHL GSPITTVPKL SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM 250 260 270 280 290 300 NAIQMEEEES GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI 310 320 330 340 350 360 IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC EFSSERGPRM 370 380 390 400 410 420 CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC DPQSYCPMLH QDHQAELLSW 430 440 RSQSKAQEGE RQLQGQIALL VKGASPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis." Wu S., Wolgemuth D.J. Dev. Biol. 170:195-206(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes." Nargi J.L., Woodford-Thomas T.A. Immunogenetics 39:99-108(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15562 mRNA. Translation: AAA74912.1. L16926 mRNA. Translation: AAA37409.1. BC004669 mRNA. Translation: AAH04669.1.
IPI	IPI00266454.
PIR	I49126.
RefSeq	NP_033990.2. NM_009860.2.
UniGene	Mm.286602.
3D structure databases
ProteinModelPortal	P48967.
SMR	P48967. Positions 255-421.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48967. 1 interaction.
PTM databases
PhosphoSite	P48967.
Proteomic databases
PaxDb	P48967.
PRIDE	P48967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
GeneID	12532.
KEGG	mmu:12532.
UCSC	uc008elf.1. mouse.
Organism-specific databases
CTD	995.
MGI	MGI:88350. Cdc25c.
Phylogenomic databases
eggNOG	COG5105.
HOGENOM	HOG000082672.
HOVERGEN	HBG052501.
InParanoid	P48967.
KO	K05867.
OMA	FRSNQRK.
OrthoDB	EOG47D9G1.
Gene expression databases
ArrayExpress	P48967.
Bgee	P48967.
CleanEx	MM_CDC25C.
Genevestigator	P48967.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF06617. M-inducer_phosp. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281566.
SOURCE	Search...

Entry information

Entry name

MPIP3_MOUSE

Accession

Primary (citable) accession number: P48967
Secondary accession number(s): Q99KG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 27, 2002
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program