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P48967

- MPIP3_MOUSE

UniProt

P48967 - MPIP3_MOUSE

Protein

M-phase inducer phosphatase 3

Gene

Cdc25c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity. May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei350 – 3501By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: Ensembl
    2. mitotic nuclear division Source: UniProtKB-KW
    3. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_209377. Cyclin B2 mediated events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 3 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25C
    Gene namesi
    Name:Cdc25c
    Synonyms:Cdc25m1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:88350. Cdc25c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 447446M-phase inducer phosphatase 3PRO_0000198648Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei38 – 381PhosphoserineBy similarity
    Modified residuei56 – 561PhosphoserineBy similarity
    Modified residuei60 – 601PhosphoserineBy similarity
    Modified residuei63 – 631PhosphoserineBy similarity
    Modified residuei66 – 661Phosphothreonine; by CDK1By similarity
    Modified residuei128 – 1281PhosphoserineBy similarity
    Modified residuei129 – 1291Phosphothreonine; by CDK1By similarity
    Modified residuei192 – 1921Phosphoserine; by CDK1By similarity
    Modified residuei213 – 2131Phosphoserine; by PLK3By similarity
    Modified residuei220 – 2201Phosphoserine; by PLK3By similarity
    Modified residuei445 – 4451PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity By similarity. Phosphorylated by CHEK1 and MAPKAPK2. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase activity. Phosphorylation by PLK3 at Ser-213 promotes nuclear translocation. Ser-220 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP48967.
    PRIDEiP48967.

    PTM databases

    PhosphoSiteiP48967.

    Expressioni

    Tissue specificityi

    Spleen and thymus.

    Gene expression databases

    ArrayExpressiP48967.
    BgeeiP48967.
    CleanExiMM_CDC25C.
    GenevestigatoriP48967.

    Interactioni

    Subunit structurei

    Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-128 and/or Thr-129, interacts with PLK1.By similarity

    Protein-protein interaction databases

    IntActiP48967. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP48967.
    SMRiP48967. Positions 255-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini294 – 401108RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    HOGENOMiHOG000082672.
    HOVERGENiHBG052501.
    InParanoidiP48967.
    KOiK05867.
    OMAiTVSLCDI.
    OrthoDBiEOG7288R1.
    PhylomeDBiP48967.
    TreeFamiTF101056.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48967-1 [UniParc]FASTAAdd to Basket

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    MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK    50
    DKLHDSANLS ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS 100
    SGPLDRQLTG KDFHQDLMKG IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK 150
    ENINTSLKAL EWEAPRTPRF RKMPGGPLTS PLCELEMKHL GSPITTVPKL 200
    SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM NAIQMEEEES 250
    GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI 300
    IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC 350
    EFSSERGPRM CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC 400
    DPQSYCPMLH QDHQAELLSW RSQSKAQEGE RQLQGQIALL VKGASPQ 447
    Length:447
    Mass (Da):50,046
    Last modified:May 27, 2002 - v2
    Checksum:i513E51BAB3D1A39E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti153 – 16513INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409. (PubMed:8276463)CuratedAdd
    BLAST
    Sequence conflicti336 – 3361V → L in AAA74912. (PubMed:7601309)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15562 mRNA. Translation: AAA74912.1.
    L16926 mRNA. Translation: AAA37409.1.
    BC004669 mRNA. Translation: AAH04669.1.
    CCDSiCCDS37759.1.
    PIRiI49126.
    RefSeqiNP_033990.2. NM_009860.2.
    XP_006525613.1. XM_006525550.1.
    UniGeneiMm.286602.

    Genome annotation databases

    EnsembliENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
    GeneIDi12532.
    KEGGimmu:12532.
    UCSCiuc008elf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15562 mRNA. Translation: AAA74912.1 .
    L16926 mRNA. Translation: AAA37409.1 .
    BC004669 mRNA. Translation: AAH04669.1 .
    CCDSi CCDS37759.1.
    PIRi I49126.
    RefSeqi NP_033990.2. NM_009860.2.
    XP_006525613.1. XM_006525550.1.
    UniGenei Mm.286602.

    3D structure databases

    ProteinModelPortali P48967.
    SMRi P48967. Positions 255-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48967. 1 interaction.

    PTM databases

    PhosphoSitei P48967.

    Proteomic databases

    PaxDbi P48967.
    PRIDEi P48967.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000060710 ; ENSMUSP00000055427 ; ENSMUSG00000044201 .
    GeneIDi 12532.
    KEGGi mmu:12532.
    UCSCi uc008elf.1. mouse.

    Organism-specific databases

    CTDi 995.
    MGIi MGI:88350. Cdc25c.

    Phylogenomic databases

    eggNOGi COG5105.
    HOGENOMi HOG000082672.
    HOVERGENi HBG052501.
    InParanoidi P48967.
    KOi K05867.
    OMAi TVSLCDI.
    OrthoDBi EOG7288R1.
    PhylomeDBi P48967.
    TreeFami TF101056.

    Enzyme and pathway databases

    Reactomei REACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_209377. Cyclin B2 mediated events.

    Miscellaneous databases

    NextBioi 281566.
    PROi P48967.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48967.
    Bgeei P48967.
    CleanExi MM_CDC25C.
    Genevestigatori P48967.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis."
      Wu S., Wolgemuth D.J.
      Dev. Biol. 170:195-206(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes."
      Nargi J.L., Woodford-Thomas T.A.
      Immunogenetics 39:99-108(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiMPIP3_MOUSE
    AccessioniPrimary (citable) accession number: P48967
    Secondary accession number(s): Q99KG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3