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P48967

- MPIP3_MOUSE

UniProt

P48967 - MPIP3_MOUSE

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Protein

M-phase inducer phosphatase 3

Gene
Cdc25c, Cdc25m1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity. May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei350 – 3501 By similarity

GO - Molecular functioni

  1. protein binding Source: MGI
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: Ensembl
  2. mitotic nuclear division Source: UniProtKB-KW
  3. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_206803. Cyclin A/B1 associated events during G2/M transition.
REACT_209377. Cyclin B2 mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:Cdc25c
Synonyms:Cdc25m1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:88350. Cdc25c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
  3. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 447446M-phase inducer phosphatase 3PRO_0000198648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei38 – 381Phosphoserine By similarity
Modified residuei56 – 561Phosphoserine By similarity
Modified residuei60 – 601Phosphoserine By similarity
Modified residuei63 – 631Phosphoserine By similarity
Modified residuei66 – 661Phosphothreonine; by CDK1 By similarity
Modified residuei128 – 1281Phosphoserine By similarity
Modified residuei129 – 1291Phosphothreonine; by CDK1 By similarity
Modified residuei192 – 1921Phosphoserine; by CDK1 By similarity
Modified residuei213 – 2131Phosphoserine; by PLK3 By similarity
Modified residuei220 – 2201Phosphoserine; by PLK3 By similarity
Modified residuei445 – 4451Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity By similarity. Phosphorylated by CHEK1 and MAPKAPK2. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase activity. Phosphorylation by PLK3 at Ser-213 promotes nuclear translocation. Ser-220 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48967.
PRIDEiP48967.

PTM databases

PhosphoSiteiP48967.

Expressioni

Tissue specificityi

Spleen and thymus.

Gene expression databases

ArrayExpressiP48967.
BgeeiP48967.
CleanExiMM_CDC25C.
GenevestigatoriP48967.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins By similarity. When phosphorylated at Ser-128 and/or Thr-129, interacts with PLK1 By similarity.

Protein-protein interaction databases

IntActiP48967. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP48967.
SMRiP48967. Positions 255-421.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini294 – 401108RhodaneseAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP48967.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
PhylomeDBiP48967.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48967-1 [UniParc]FASTAAdd to Basket

« Hide

MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK    50
DKLHDSANLS ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS 100
SGPLDRQLTG KDFHQDLMKG IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK 150
ENINTSLKAL EWEAPRTPRF RKMPGGPLTS PLCELEMKHL GSPITTVPKL 200
SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM NAIQMEEEES 250
GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI 300
IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC 350
EFSSERGPRM CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC 400
DPQSYCPMLH QDHQAELLSW RSQSKAQEGE RQLQGQIALL VKGASPQ 447
Length:447
Mass (Da):50,046
Last modified:May 27, 2002 - v2
Checksum:i513E51BAB3D1A39E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 16513INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409. 1 PublicationAdd
BLAST
Sequence conflicti336 – 3361V → L in AAA74912. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15562 mRNA. Translation: AAA74912.1.
L16926 mRNA. Translation: AAA37409.1.
BC004669 mRNA. Translation: AAH04669.1.
CCDSiCCDS37759.1.
PIRiI49126.
RefSeqiNP_033990.2. NM_009860.2.
XP_006525613.1. XM_006525550.1.
UniGeneiMm.286602.

Genome annotation databases

EnsembliENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
GeneIDi12532.
KEGGimmu:12532.
UCSCiuc008elf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15562 mRNA. Translation: AAA74912.1 .
L16926 mRNA. Translation: AAA37409.1 .
BC004669 mRNA. Translation: AAH04669.1 .
CCDSi CCDS37759.1.
PIRi I49126.
RefSeqi NP_033990.2. NM_009860.2.
XP_006525613.1. XM_006525550.1.
UniGenei Mm.286602.

3D structure databases

ProteinModelPortali P48967.
SMRi P48967. Positions 255-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48967. 1 interaction.

PTM databases

PhosphoSitei P48967.

Proteomic databases

PaxDbi P48967.
PRIDEi P48967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000060710 ; ENSMUSP00000055427 ; ENSMUSG00000044201 .
GeneIDi 12532.
KEGGi mmu:12532.
UCSCi uc008elf.1. mouse.

Organism-specific databases

CTDi 995.
MGIi MGI:88350. Cdc25c.

Phylogenomic databases

eggNOGi COG5105.
HOGENOMi HOG000082672.
HOVERGENi HBG052501.
InParanoidi P48967.
KOi K05867.
OMAi TVSLCDI.
OrthoDBi EOG7288R1.
PhylomeDBi P48967.
TreeFami TF101056.

Enzyme and pathway databases

Reactomei REACT_206803. Cyclin A/B1 associated events during G2/M transition.
REACT_209377. Cyclin B2 mediated events.

Miscellaneous databases

NextBioi 281566.
PROi P48967.
SOURCEi Search...

Gene expression databases

ArrayExpressi P48967.
Bgeei P48967.
CleanExi MM_CDC25C.
Genevestigatori P48967.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis."
    Wu S., Wolgemuth D.J.
    Dev. Biol. 170:195-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes."
    Nargi J.L., Woodford-Thomas T.A.
    Immunogenetics 39:99-108(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiMPIP3_MOUSE
AccessioniPrimary (citable) accession number: P48967
Secondary accession number(s): Q99KG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 27, 2002
Last modified: September 3, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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