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Protein

M-phase inducer phosphatase 3

Gene

Cdc25c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase (By similarity). May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei350 – 3501By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_274616. Activation of ATR in response to replication stress.
REACT_291378. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_323594. Cyclin B2 mediated events.
REACT_349749. Cyclin A/B1 associated events during G2/M transition.
REACT_353875. Polo-like kinase mediated events.
REACT_358414. RHO GTPases activate PKNs.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:Cdc25c
Synonyms:Cdc25m1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:88350. Cdc25c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 447446M-phase inducer phosphatase 3PRO_0000198648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei38 – 381PhosphoserineBy similarity
Modified residuei56 – 561PhosphoserineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei63 – 631PhosphoserineBy similarity
Modified residuei66 – 661Phosphothreonine; by CDK1By similarity
Modified residuei128 – 1281PhosphoserineBy similarity
Modified residuei129 – 1291PhosphothreonineBy similarity
Modified residuei192 – 1921Phosphoserine; by CDK1By similarity
Modified residuei213 – 2131Phosphoserine; by PLK3By similarity
Modified residuei220 – 2201Phosphoserine; by PLK3By similarity
Modified residuei445 – 4451PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity (By similarity). Phosphorylated by CHEK1 and MAPKAPK2. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase activity. Phosphorylation by PLK3 at Ser-213 promotes nuclear translocation. Ser-220 is a minor phosphorylation site (By similarity). Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48967.
PRIDEiP48967.

PTM databases

PhosphoSiteiP48967.

Expressioni

Tissue specificityi

Spleen and thymus.

Gene expression databases

BgeeiP48967.
CleanExiMM_CDC25C.
ExpressionAtlasiP48967. baseline and differential.
GenevestigatoriP48967.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-128 and/or Thr-129, interacts with PLK1.By similarity

Protein-protein interaction databases

IntActiP48967. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP48967.
SMRiP48967. Positions 255-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini294 – 401108RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP48967.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
PhylomeDBiP48967.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK
60 70 80 90 100
DKLHDSANLS ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS
110 120 130 140 150
SGPLDRQLTG KDFHQDLMKG IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK
160 170 180 190 200
ENINTSLKAL EWEAPRTPRF RKMPGGPLTS PLCELEMKHL GSPITTVPKL
210 220 230 240 250
SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM NAIQMEEEES
260 270 280 290 300
GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI
310 320 330 340 350
IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC
360 370 380 390 400
EFSSERGPRM CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC
410 420 430 440
DPQSYCPMLH QDHQAELLSW RSQSKAQEGE RQLQGQIALL VKGASPQ
Length:447
Mass (Da):50,046
Last modified:May 27, 2002 - v2
Checksum:i513E51BAB3D1A39E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 16513INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409 (PubMed:8276463).CuratedAdd
BLAST
Sequence conflicti336 – 3361V → L in AAA74912 (PubMed:7601309).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15562 mRNA. Translation: AAA74912.1.
L16926 mRNA. Translation: AAA37409.1.
BC004669 mRNA. Translation: AAH04669.1.
CCDSiCCDS37759.1.
PIRiI49126.
RefSeqiNP_033990.2. NM_009860.3.
XP_006525613.1. XM_006525550.2.
UniGeneiMm.286602.

Genome annotation databases

EnsembliENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
GeneIDi12532.
KEGGimmu:12532.
UCSCiuc008elf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15562 mRNA. Translation: AAA74912.1.
L16926 mRNA. Translation: AAA37409.1.
BC004669 mRNA. Translation: AAH04669.1.
CCDSiCCDS37759.1.
PIRiI49126.
RefSeqiNP_033990.2. NM_009860.3.
XP_006525613.1. XM_006525550.2.
UniGeneiMm.286602.

3D structure databases

ProteinModelPortaliP48967.
SMRiP48967. Positions 255-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48967. 1 interaction.

PTM databases

PhosphoSiteiP48967.

Proteomic databases

PaxDbiP48967.
PRIDEiP48967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
GeneIDi12532.
KEGGimmu:12532.
UCSCiuc008elf.1. mouse.

Organism-specific databases

CTDi995.
MGIiMGI:88350. Cdc25c.

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP48967.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
PhylomeDBiP48967.
TreeFamiTF101056.

Enzyme and pathway databases

ReactomeiREACT_274616. Activation of ATR in response to replication stress.
REACT_291378. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_323594. Cyclin B2 mediated events.
REACT_349749. Cyclin A/B1 associated events during G2/M transition.
REACT_353875. Polo-like kinase mediated events.
REACT_358414. RHO GTPases activate PKNs.

Miscellaneous databases

NextBioi281566.
PROiP48967.
SOURCEiSearch...

Gene expression databases

BgeeiP48967.
CleanExiMM_CDC25C.
ExpressionAtlasiP48967. baseline and differential.
GenevestigatoriP48967.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis."
    Wu S., Wolgemuth D.J.
    Dev. Biol. 170:195-206(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes."
    Nargi J.L., Woodford-Thomas T.A.
    Immunogenetics 39:99-108(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiMPIP3_MOUSE
AccessioniPrimary (citable) accession number: P48967
Secondary accession number(s): Q99KG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 27, 2002
Last modified: May 27, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.