Reviewed, UniProtKB/Swiss-Prot P48967 (MPIP3_MOUSE)

Names and origin

Protein names

Recommended name:
    M-phase inducer phosphatase 3
    EC=3.1.3.48
Alternative name(s):
    Dual specificity phosphatase Cdc25C

Gene names

Name:	Cdc25c
Synonyms:	Cdc25m1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity. May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Nucleus.
Tissue specificity	Spleen and thymus.
Post-translational modification	Phosphorylated by PLK4 By similarity. Phosphorylated by CHK1. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cdc2	P11440	1	EBI-1216048,EBI-846949

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	M-phase inducer phosphatase 3		PRO_0000198648
Regions
	Domain	294 – 401	108	Rhodanese
Sites
	Active site	350	1	By similarity
Amino acid modifications
	Modified residue	38	1	Phosphoserine By similarity
	Modified residue	56	1	Phosphoserine By similarity
	Modified residue	60	1	Phosphoserine By similarity
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	66	1	Phosphothreonine By similarity
	Modified residue	192	1	Phosphoserine By similarity
	Modified residue	445	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	153 – 165	13	INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409. Ref.2
	Sequence conflict	336	1	V → L in AAA74912. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48967-1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 513E51BAB3D1A39E	FASTA	447	50,046
	10 20 30 40 50 60 MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK DKLHDSANLS 70 80 90 100 110 120 ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS SGPLDRQLTG KDFHQDLMKG 130 140 150 160 170 180 IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK ENINTSLKAL EWEAPRTPRF RKMPGGPLTS 190 200 210 220 230 240 PLCELEMKHL GSPITTVPKL SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM 250 260 270 280 290 300 NAIQMEEEES GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI 310 320 330 340 350 360 IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC EFSSERGPRM 370 380 390 400 410 420 CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC DPQSYCPMLH QDHQAELLSW 430 440 RSQSKAQEGE RQLQGQIALL VKGASPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis." Wu S., Wolgemuth D.J. Dev. Biol. 170:195-206(1995) [PubMed: 7601309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes." Nargi J.L., Woodford-Thomas T.A. Immunogenetics 39:99-108(1994) [PubMed: 8276463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15562 mRNA. Translation: AAA74912.1. L16926 mRNA. Translation: AAA37409.1. BC004669 mRNA. Translation: AAH04669.1.
IPI	IPI00266454.
PIR	I49126.
RefSeq	NP_033990.2.
UniGene	Mm.286602
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P48967. 1 interaction.
STRING	P48967.
PTM databases
PhosphoSite	P48967.
Proteomic databases
PRIDE	P48967.
Genome annotation databases
Ensembl	ENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201; Mus musculus. [Genome view]
GeneID	12532.
KEGG	mmu:12532.
UCSC	uc008elf.1. mouse.
Organism-specific databases
CTD	12532.
MGI	MGI:88350. Cdc25c.
Phylogenomic databases
HOVERGEN	P48967.
OMA	LDTQKRI
OrthoDB	EOG9J13V3
Enzyme and pathway databases
BRENDA	3.1.3.48. 244.
Gene expression databases
ArrayExpress	P48967.
Bgee	P48967.
CleanEx	MM_CDC25C.
Genevestigator	P48967.
Family and domain databases
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
PANTHER	PTHR10828. MPI_Phosphatase. 1 hit.
Pfam	PF06617. M-inducer_phosp. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281566.
SOURCE	Search...

Entry information

Entry name

MPIP3_MOUSE

Accession

Primary (citable) accession number: P48967
Secondary accession number(s): Q99KG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 27, 2002
Last modified:	November 24, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)