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P48967 (MPIP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase 3

EC=3.1.3.48
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene names
Name:Cdc25c
Synonyms:Cdc25m1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity. May be involved in regulating the proliferation of T-lymphocytes following cytokine stimulation.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with MAPK14 and 14-3-3 proteins By similarity. When phosphorylated at Ser-128 and/or Thr-129, interacts with PLK1 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Spleen and thymus.

Post-translational modification

Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity By similarity. Phosphorylated by CHEK1 and MAPKAPK2. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase activity. Phosphorylation by PLK3 at Ser-213 promotes nuclear translocation. Ser-220 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 447446M-phase inducer phosphatase 3
PRO_0000198648

Regions

Domain294 – 401108Rhodanese

Sites

Active site3501 By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue381Phosphoserine By similarity
Modified residue561Phosphoserine By similarity
Modified residue601Phosphoserine By similarity
Modified residue631Phosphoserine By similarity
Modified residue661Phosphothreonine; by CDK1 By similarity
Modified residue1281Phosphoserine By similarity
Modified residue1291Phosphothreonine; by CDK1 By similarity
Modified residue1921Phosphoserine; by CDK1 By similarity
Modified residue2131Phosphoserine; by PLK3 By similarity
Modified residue2201Phosphoserine; by PLK3 By similarity
Modified residue4451Phosphoserine By similarity

Experimental info

Sequence conflict153 – 16513INTSL…EWEAP → AAFLLLSLQLNDAGPSCADK HQFKGIGMGGT in AAA37409. Ref.2
Sequence conflict3361V → L in AAA74912. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48967 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: 513E51BAB3D1A39E

FASTA44750,046
        10         20         30         40         50         60 
MSTGPIPPAS EEGSFVSAPS FRSKQRKILH LLLERNTSFT IRSDFPESPK DKLHDSANLS 

        70         80         90        100        110        120 
ILSGGTPKCC LDLSNLSSGE MSASPLTTSA DLEDNGSLDS SGPLDRQLTG KDFHQDLMKG 

       130        140        150        160        170        180 
IPVQLLCSTP NAMNHGHRKK IAKRSTSAHK ENINTSLKAL EWEAPRTPRF RKMPGGPLTS 

       190        200        210        220        230        240 
PLCELEMKHL GSPITTVPKL SQNVKLEDQE RISEDPMECS LGDQDAKGLS LRKMVPLCDM 

       250        260        270        280        290        300 
NAIQMEEEES GSELLIGDFS KVCVLPTVPG KHPDLKYISP DTVAALLSGK FQSVIERFYI 

       310        320        330        340        350        360 
IDCRYPYEYL GGHILGALNL HSQKELHEFF LRKPVVPLDI QKRVIIVFLC EFSSERGPRM 

       370        380        390        400        410        420 
CRSLREKDRA LNQYPALYYP ELYILKGGYR DFFPEYMELC DPQSYCPMLH QDHQAELLSW 

       430        440 
RSQSKAQEGE RQLQGQIALL VKGASPQ 

« Hide

References

« Hide 'large scale' references
[1]"The distinct and developmentally regulated patterns of expression of members of the mouse Cdc25 gene family suggest differential functions during gametogenesis."
Wu S., Wolgemuth D.J.
Dev. Biol. 170:195-206(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of a cdc25 phosphatase from mouse lymphocytes."
Nargi J.L., Woodford-Thomas T.A.
Immunogenetics 39:99-108(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15562 mRNA. Translation: AAA74912.1.
L16926 mRNA. Translation: AAA37409.1.
BC004669 mRNA. Translation: AAH04669.1.
PIRI49126.
RefSeqNP_033990.2. NM_009860.2.
XP_006525613.1. XM_006525550.1.
UniGeneMm.286602.

3D structure databases

ProteinModelPortalP48967.
SMRP48967. Positions 255-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48967. 1 interaction.

PTM databases

PhosphoSiteP48967.

Proteomic databases

PaxDbP48967.
PRIDEP48967.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000060710; ENSMUSP00000055427; ENSMUSG00000044201.
GeneID12532.
KEGGmmu:12532.
UCSCuc008elf.1. mouse.

Organism-specific databases

CTD995.
MGIMGI:88350. Cdc25c.

Phylogenomic databases

eggNOGCOG5105.
HOGENOMHOG000082672.
HOVERGENHBG052501.
InParanoidP48967.
KOK05867.
OMATVSLCDI.
OrthoDBEOG7288R1.
PhylomeDBP48967.
TreeFamTF101056.

Gene expression databases

ArrayExpressP48967.
BgeeP48967.
CleanExMM_CDC25C.
GenevestigatorP48967.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281566.
PROP48967.
SOURCESearch...

Entry information

Entry nameMPIP3_MOUSE
AccessionPrimary (citable) accession number: P48967
Secondary accession number(s): Q99KG6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 27, 2002
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot