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Protein

M-phase inducer phosphatase 2

Gene

Cdc25b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Stimulated by B-type cyclins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei481 – 4811By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene namesi
Name:Cdc25b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621500. Cdc25b.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574M-phase inducer phosphatase 2PRO_0000198646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by MELKBy similarity
Modified residuei246 – 2461PhosphoserineBy similarity
Modified residuei319 – 3191Phosphoserine; by MAPKAPK2 and MELK; alternateBy similarity
Modified residuei319 – 3191Phosphoserine; by MELK and MAPK14; alternateBy similarity
Modified residuei349 – 3491Phosphoserine; by AURKABy similarity
Modified residuei370 – 3701Phosphoserine; by BRSK1 and MAPK14By similarity

Post-translational modificationi

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-349 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-166 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-319 and Ser-370 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48966.
PRIDEiP48966.

PTM databases

PhosphoSiteiP48966.

Expressioni

Gene expression databases

GenevestigatoriP48966.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028864.

Structurei

3D structure databases

ProteinModelPortaliP48966.
SMRiP48966. Positions 381-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini425 – 532108RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP48966.
KOiK05866.
PhylomeDBiP48966.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVPPQKSAP GSALSTARVL GGIQRPRHLS GFGFGSDGLL GSPERAASSS
60 70 80 90 100
PVTTLTQTMY NLAGLGSETP KTQVGSLSFQ NRLTDLSLSR RTSECSLSSE
110 120 130 140 150
SSESSDAGLC MDSPSPMDPQ TAERTFEQAI QAASRVIQKM QFTIKASVFA
160 170 180 190 200
SEAAGHSPVL QNITNSQALD SWEKDEAGYR AASSPGEDKE NDGYIFKMPQ
210 220 230 240 250
KLPHSSSARA LAEWASRREA FTQRPSSAPD LMCLTTDGKM DVEEASPVAQ
260 270 280 290 300
SSSLTPVERA CEEDDGFVDI LESDLKDDDM VPAGMENLIS APLVKKLDKE
310 320 330 340 350
EEQDLIMFSK CQRLFRSPSM PCSVIRPILK RLERPHDRDV PVLSKRRKSG
360 370 380 390 400
TPLEEQQLEE PKARVFRSKS LCHEIESILD SDHRGLIGDY SKAFLLQTVD
410 420 430 440 450
GKHQDLKYIS PETMVALLTG KFSNIVEKFV IVDCRYPYEY EGGHIKNAVN
460 470 480 490 500
LPLEPDAETF LLKHPITPCN LDKRIILIFH CEFSSERGPR MCRFIRERDR
510 520 530 540 550
AANDYPSLYY PEMYILKGGY KEFFPQHPNF CEPQDYRPMN HAAFRDELRN
560 570
FRLKTRSWAG ERSTTQLCSR LQDQ
Length:574
Mass (Da):64,287
Last modified:January 31, 1996 - v1
Checksum:i9367CE203B15FAAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16237 mRNA. Translation: BAA03762.1.
RefSeqiNP_598256.1. NM_133572.1.
UniGeneiRn.11312.

Genome annotation databases

GeneIDi171103.
KEGGirno:171103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16237 mRNA. Translation: BAA03762.1.
RefSeqiNP_598256.1. NM_133572.1.
UniGeneiRn.11312.

3D structure databases

ProteinModelPortaliP48966.
SMRiP48966. Positions 381-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028864.

PTM databases

PhosphoSiteiP48966.

Proteomic databases

PaxDbiP48966.
PRIDEiP48966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171103.
KEGGirno:171103.

Organism-specific databases

CTDi994.
RGDi621500. Cdc25b.

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP48966.
KOiK05866.
PhylomeDBiP48966.

Miscellaneous databases

NextBioi621786.
PROiP48966.

Gene expression databases

GenevestigatoriP48966.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cdc25A is a novel phosphatase functioning early in the cell cycle."
    Jinno S., Suto K., Nagata A., Igarashi M., Kanaoka Y., Nojima H., Okayama H.
    EMBO J. 13:1549-1556(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NRK49F.

Entry informationi

Entry nameiMPIP2_RAT
AccessioniPrimary (citable) accession number: P48966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: January 31, 1996
Last modified: March 31, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.