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P48966

- MPIP2_RAT

UniProt

P48966 - MPIP2_RAT

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Protein

M-phase inducer phosphatase 2

Gene
Cdc25b
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Stimulated by B-type cyclins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei481 – 4811 By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. mitotic nuclear division Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene namesi
Name:Cdc25b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621500. Cdc25b.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574M-phase inducer phosphatase 2PRO_0000198646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by MELK By similarity
Modified residuei246 – 2461Phosphoserine By similarity
Modified residuei319 – 3191Phosphoserine; by MAPKAPK2 and MELK; alternate By similarity
Modified residuei319 – 3191Phosphoserine; by MELK and MAPK14; alternate By similarity
Modified residuei349 – 3491Phosphoserine; by AURKA By similarity
Modified residuei370 – 3701Phosphoserine; by BRSK1 and MAPK14 By similarity

Post-translational modificationi

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-349 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-166 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-319 and Ser-370 by MAPK14 is required for binding to 14-3-3 proteins By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48966.
PRIDEiP48966.

PTM databases

PhosphoSiteiP48966.

Expressioni

Gene expression databases

GenevestigatoriP48966.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028864.

Structurei

3D structure databases

ProteinModelPortaliP48966.
SMRiP48966. Positions 381-558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini425 – 532108RhodaneseAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
KOiK05866.
PhylomeDBiP48966.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48966-1 [UniParc]FASTAAdd to Basket

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MEVPPQKSAP GSALSTARVL GGIQRPRHLS GFGFGSDGLL GSPERAASSS    50
PVTTLTQTMY NLAGLGSETP KTQVGSLSFQ NRLTDLSLSR RTSECSLSSE 100
SSESSDAGLC MDSPSPMDPQ TAERTFEQAI QAASRVIQKM QFTIKASVFA 150
SEAAGHSPVL QNITNSQALD SWEKDEAGYR AASSPGEDKE NDGYIFKMPQ 200
KLPHSSSARA LAEWASRREA FTQRPSSAPD LMCLTTDGKM DVEEASPVAQ 250
SSSLTPVERA CEEDDGFVDI LESDLKDDDM VPAGMENLIS APLVKKLDKE 300
EEQDLIMFSK CQRLFRSPSM PCSVIRPILK RLERPHDRDV PVLSKRRKSG 350
TPLEEQQLEE PKARVFRSKS LCHEIESILD SDHRGLIGDY SKAFLLQTVD 400
GKHQDLKYIS PETMVALLTG KFSNIVEKFV IVDCRYPYEY EGGHIKNAVN 450
LPLEPDAETF LLKHPITPCN LDKRIILIFH CEFSSERGPR MCRFIRERDR 500
AANDYPSLYY PEMYILKGGY KEFFPQHPNF CEPQDYRPMN HAAFRDELRN 550
FRLKTRSWAG ERSTTQLCSR LQDQ 574
Length:574
Mass (Da):64,287
Last modified:February 1, 1996 - v1
Checksum:i9367CE203B15FAAD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16237 mRNA. Translation: BAA03762.1.
RefSeqiNP_598256.1. NM_133572.1.
UniGeneiRn.11312.

Genome annotation databases

GeneIDi171103.
KEGGirno:171103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16237 mRNA. Translation: BAA03762.1 .
RefSeqi NP_598256.1. NM_133572.1.
UniGenei Rn.11312.

3D structure databases

ProteinModelPortali P48966.
SMRi P48966. Positions 381-558.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000028864.

PTM databases

PhosphoSitei P48966.

Proteomic databases

PaxDbi P48966.
PRIDEi P48966.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 171103.
KEGGi rno:171103.

Organism-specific databases

CTDi 994.
RGDi 621500. Cdc25b.

Phylogenomic databases

eggNOGi COG5105.
HOGENOMi HOG000082672.
HOVERGENi HBG052501.
KOi K05866.
PhylomeDBi P48966.

Miscellaneous databases

NextBioi 621786.
PROi P48966.

Gene expression databases

Genevestigatori P48966.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cdc25A is a novel phosphatase functioning early in the cell cycle."
    Jinno S., Suto K., Nagata A., Igarashi M., Kanaoka Y., Nojima H., Okayama H.
    EMBO J. 13:1549-1556(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NRK49F.

Entry informationi

Entry nameiMPIP2_RAT
AccessioniPrimary (citable) accession number: P48966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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