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Protein

ADP/ATP translocase 1

Gene

Slc25a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801NucleotideBy similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic mitochondrial changes Source: MGI
  • negative regulation of necroptotic process Source: MGI
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-422356. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP/ATP translocase 1
Alternative name(s):
ADP,ATP carrier protein 1
ADP,ATP carrier protein, heart/skeletal muscle isoform T1
Adenine nucleotide translocator 1
Short name:
ANT 1
Solute carrier family 25 member 4
mANC1
Gene namesi
Name:Slc25a4
Synonyms:Anc1, Ant1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1353495. Slc25a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 3935Helical; Name=1By similarityAdd
BLAST
Transmembranei75 – 10026Helical; Name=2By similarityAdd
BLAST
Transmembranei109 – 14335Helical; Name=3By similarityAdd
BLAST
Transmembranei176 – 20227Helical; Name=4By similarityAdd
BLAST
Transmembranei207 – 24135Helical; Name=5By similarityAdd
BLAST
Transmembranei273 – 29826Helical; Name=6By similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: MGI
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 298297ADP/ATP translocase 1PRO_0000090575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei147 – 1471N6-succinyllysineCombined sources
Modified residuei149 – 1491PhosphoserineBy similarity
Modified residuei150 – 1501PhosphoserineBy similarity
Modified residuei160 – 1601S-nitrosocysteineBy similarity
Modified residuei245 – 2451N6-succinyllysineCombined sources
Modified residuei272 – 2721N6-succinyllysineCombined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugation

Proteomic databases

EPDiP48962.
MaxQBiP48962.
PaxDbiP48962.
PRIDEiP48962.
TopDownProteomicsiP48962.

PTM databases

iPTMnetiP48962.
PhosphoSiteiP48962.
SwissPalmiP48962.

Expressioni

Gene expression databases

BgeeiP48962.
GenevisibleiP48962. MM.

Interactioni

Subunit structurei

Homodimer. Found in a complex with ARL2, ARL2BP and SLC25A4. Interacts with ARL2BP.1 Publication

Protein-protein interaction databases

BioGridi198105. 5 interactions.
IntActiP48962. 15 interactions.
MINTiMINT-262910.
STRINGi10090.ENSMUSP00000034049.

Structurei

3D structure databases

ProteinModelPortaliP48962.
SMRiP48962. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 9893Solcar 1Add
BLAST
Repeati111 – 20191Solcar 2Add
BLAST
Repeati212 – 29786Solcar 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi235 – 2406Substrate recognitionBy similarity

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0749. Eukaryota.
ENOG410XNW0. LUCA.
GeneTreeiENSGT00390000011543.
HOGENOMiHOG000165727.
HOVERGENiHBG108348.
InParanoidiP48962.
KOiK05863.
OMAiGSSQREF.
OrthoDBiEOG7T1RBR.
PhylomeDBiP48962.
TreeFamiTF300743.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002113. Aden_trnslctor.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00927. ADPTRNSLCASE.
PR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDQALSFLK DFLAGGIAAA VSKTAVAPIE RVKLLLQVQH ASKQISAEKQ
60 70 80 90 100
YKGIIDCVVR IPKEQGFLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL
110 120 130 140 150
GGVDRHKQFW RYFAGNLASG GAAGATSLCF VYPLDFARTR LAADVGKGSS
160 170 180 190 200
QREFNGLGDC LTKIFKSDGL KGLYQGFSVS VQGIIIYRAA YFGVYDTAKG
210 220 230 240 250
MLPDPKNVHI IVSWMIAQSV TAVAGLVSYP FDTVRRRMMM QSGRKGADIM
260 270 280 290
YTGTLDCWRK IAKDEGANAF FKGAWSNVLR GMGGAFVLVL YDEIKKYV
Length:298
Mass (Da):32,904
Last modified:January 23, 2007 - v4
Checksum:i3A849FEAB0981462
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361F → L in AAC52837 (PubMed:8903724).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27315 mRNA. Translation: AAC52837.1.
X74510 mRNA. Translation: CAA52616.1.
AF240002 Genomic DNA. Translation: AAF64470.1.
BC003791 mRNA. Translation: AAH03791.1.
BC026925 mRNA. Translation: AAH26925.1.
CCDSiCCDS40333.1.
PIRiS37210.
RefSeqiNP_031476.3. NM_007450.4.
UniGeneiMm.16228.

Genome annotation databases

EnsembliENSMUST00000034049; ENSMUSP00000034049; ENSMUSG00000031633.
GeneIDi11739.
KEGGimmu:11739.
UCSCiuc009lpz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27315 mRNA. Translation: AAC52837.1.
X74510 mRNA. Translation: CAA52616.1.
AF240002 Genomic DNA. Translation: AAF64470.1.
BC003791 mRNA. Translation: AAH03791.1.
BC026925 mRNA. Translation: AAH26925.1.
CCDSiCCDS40333.1.
PIRiS37210.
RefSeqiNP_031476.3. NM_007450.4.
UniGeneiMm.16228.

3D structure databases

ProteinModelPortaliP48962.
SMRiP48962. Positions 3-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198105. 5 interactions.
IntActiP48962. 15 interactions.
MINTiMINT-262910.
STRINGi10090.ENSMUSP00000034049.

PTM databases

iPTMnetiP48962.
PhosphoSiteiP48962.
SwissPalmiP48962.

Proteomic databases

EPDiP48962.
MaxQBiP48962.
PaxDbiP48962.
PRIDEiP48962.
TopDownProteomicsiP48962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034049; ENSMUSP00000034049; ENSMUSG00000031633.
GeneIDi11739.
KEGGimmu:11739.
UCSCiuc009lpz.2. mouse.

Organism-specific databases

CTDi291.
MGIiMGI:1353495. Slc25a4.

Phylogenomic databases

eggNOGiKOG0749. Eukaryota.
ENOG410XNW0. LUCA.
GeneTreeiENSGT00390000011543.
HOGENOMiHOG000165727.
HOVERGENiHBG108348.
InParanoidiP48962.
KOiK05863.
OMAiGSSQREF.
OrthoDBiEOG7T1RBR.
PhylomeDBiP48962.
TreeFamiTF300743.

Enzyme and pathway databases

ReactomeiR-MMU-422356. Regulation of insulin secretion.

Miscellaneous databases

ChiTaRSiSlc25a4. mouse.
PROiP48962.
SOURCEiSearch...

Gene expression databases

BgeeiP48962.
GenevisibleiP48962. MM.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002113. Aden_trnslctor.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00927. ADPTRNSLCASE.
PR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rapid evolution of human pseudoautosomal genes and their mouse homologs."
    Ellison J.W., Li X., Francke U., Shapiro L.J.
    Mamm. Genome 7:25-30(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Laplace C., Costet P.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Muscle.
  3. "Expression and sequence analysis of the mouse adenine nucleotide translocase 1 and 2 genes."
    Levy S.E., Chen Y.-S., Graham B.H., Wallace D.C.
    Gene 254:57-66(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 81-92, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. "ARL2 and BART enter mitochondria and bind the adenine nucleotide transporter."
    Sharer J.D., Shern J.F., Van Valkenburgh H., Wallace D.C., Kahn R.A.
    Mol. Biol. Cell 13:71-83(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH ARL2 AND ARL2BP, INTERACTION WITH ARL2BP, TISSUE SPECIFICITY.
  7. "A proteomics approach to identify the ubiquitinated proteins in mouse heart."
    Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
    Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-10.
    Tissue: Heart.
  8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  10. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-147; LYS-245 AND LYS-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.

Entry informationi

Entry nameiADT1_MOUSE
AccessioniPrimary (citable) accession number: P48962
Secondary accession number(s): Q62164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 147 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.