P48962 (ADT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP/ATP translocase 1

Alternative name(s):
ADP,ATP carrier protein 1
ADP,ATP carrier protein, heart/skeletal muscle isoform T1
Adenine nucleotide translocator 1
Short name=ANT 1
Solute carrier family 25 member 4
mANC1

Gene names

Name:	Slc25a4
Synonyms:	Anc1, Ant1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane.
Subunit structure	Homodimer. Found in a complex with ARL2, ARL2BP and SLC25A4. Interacts with ARL2BP. Ref.6
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein. Note: The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria.
Miscellaneous	The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity.
Sequence similarities	Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 298	297	ADP/ATP translocase 1		PRO_0000090575
Regions
	Transmembrane	5 – 39	35	Helical; Name=1; By similarity
	Transmembrane	75 – 100	26	Helical; Name=2; By similarity
	Transmembrane	109 – 143	35	Helical; Name=3; By similarity
	Transmembrane	176 – 202	27	Helical; Name=4; By similarity
	Transmembrane	207 – 241	35	Helical; Name=5; By similarity
	Transmembrane	273 – 298	26	Helical; Name=6; By similarity
	Repeat	6 – 98	93	Solcar 1
	Repeat	111 – 201	91	Solcar 2
	Repeat	212 – 297	86	Solcar 3
	Motif	235 – 240	6	Substrate recognition By similarity
Sites
	Binding site	80	1	Nucleotide By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	96	1	N6-acetyllysine By similarity
	Modified residue	126	1	Phosphothreonine Ref.10
	Modified residue	127	1	Phosphoserine Ref.10
	Modified residue	191	1	Phosphotyrosine Ref.9
	Modified residue	195	1	Phosphotyrosine Ref.8
	Cross-link	10		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
Experimental info
	Sequence conflict	136	1	F → L in AAC52837. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48962 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 3A849FEAB0981462	FASTA	298	32,904
	10 20 30 40 50 60 MGDQALSFLK DFLAGGIAAA VSKTAVAPIE RVKLLLQVQH ASKQISAEKQ YKGIIDCVVR 70 80 90 100 110 120 IPKEQGFLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL GGVDRHKQFW RYFAGNLASG 130 140 150 160 170 180 GAAGATSLCF VYPLDFARTR LAADVGKGSS QREFNGLGDC LTKIFKSDGL KGLYQGFSVS 190 200 210 220 230 240 VQGIIIYRAA YFGVYDTAKG MLPDPKNVHI IVSWMIAQSV TAVAGLVSYP FDTVRRRMMM 250 260 270 280 290 QSGRKGADIM YTGTLDCWRK IAKDEGANAF FKGAWSNVLR GMGGAFVLVL YDEIKKYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rapid evolution of human pseudoautosomal genes and their mouse homologs." Ellison J.W., Li X., Francke U., Shapiro L.J. Mamm. Genome 7:25-30(1996) [PubMed: 8903724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	Laplace C., Costet P. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Muscle.
[3]	"Expression and sequence analysis of the mouse adenine nucleotide translocase 1 and 2 genes." Levy S.E., Chen Y.-S., Graham B.H., Wallace D.C. Gene 254:57-66(2000) [PubMed: 10974536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[5]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 81-92, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[6]	"ARL2 and BART enter mitochondria and bind the adenine nucleotide transporter." Sharer J.D., Shern J.F., Van Valkenburgh H., Wallace D.C., Kahn R.A. Mol. Biol. Cell 13:71-83(2002) [PubMed: 11809823] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ARL2 AND ARL2BP, INTERACTION WITH ARL2BP, TISSUE SPECIFICITY.
[7]	"A proteomics approach to identify the ubiquitinated proteins in mouse heart." Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J. Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed: 17451654] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-10, MASS SPECTROMETRY. Tissue: Heart.
[8]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-195, MASS SPECTROMETRY. Tissue: Brain.
[9]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-191, MASS SPECTROMETRY. Tissue: Brain cortex.
[10]	Lubec G., Kang S. Submitted (APR-2007) to UniProtKB Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-126 AND SER-127, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27315 mRNA. Translation: AAC52837.1. X74510 mRNA. Translation: CAA52616.1. AF240002 Genomic DNA. Translation: AAF64470.1. BC003791 mRNA. Translation: AAH03791.1. BC026925 mRNA. Translation: AAH26925.1.
IPI	IPI00115564.
PIR	S37210.
RefSeq	NP_031476.3. NM_007450.4.
UniGene	Mm.16228.
3D structure databases
ProteinModelPortal	P48962.
SMR	P48962. Positions 3-294.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48962. 6 interactions.
STRING	P48962.
PTM databases
PhosphoSite	P48962.
Proteomic databases
PRIDE	P48962.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034049; ENSMUSP00000034049; ENSMUSG00000031633.
GeneID	11739.
KEGG	mmu:11739.
UCSC	uc009lpz.1. mouse.
Organism-specific databases
CTD	291.
MGI	MGI:1353495. Slc25a4.
Phylogenomic databases
eggNOG	roNOG15102.
HOGENOM	HBG610399.
HOVERGEN	HBG108348.
InParanoid	P48962.
OMA	QREFNGL.
OrthoDB	EOG49CQ86.
PhylomeDB	P48962.
Gene expression databases
ArrayExpress	P48962.
Bgee	P48962.
Genevestigator	P48962.
GermOnline	ENSMUSG00000031633. Mus musculus.
Family and domain databases
InterPro	IPR002113. Aden_trnslctor. IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Gene3D	G3DSA:1.50.40.10. G3DSA:1.50.40.10. 1 hit.
KO	K05863.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00927. ADPTRNSLCASE. PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	279463.
SOURCE	Search...

Entry information

Entry name

ADT1_MOUSE

Accession

Primary (citable) accession number: P48962
Secondary accession number(s): Q62164

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 109 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program