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Protein

CD97 antigen

Gene

CD97

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • G-protein coupled receptor activity Source: ProtInc
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  • cell adhesion Source: ProtInc
  • cell-cell signaling Source: ProtInc
  • cell surface receptor signaling pathway Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: ProtInc
  • immune response Source: ProtInc
  • inflammatory response Source: ProtInc
  • movement of cell or subcellular component Source: ProtInc
  • neutrophil degranulation Source: Reactome

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-HSA-373080. Class B/2 (Secretin family receptors).
R-HSA-6798695. Neutrophil degranulation.
SIGNORiP48960.

Protein family/group databases

MEROPSiP02.002.
TCDBi9.A.14.6.2. the g-protein-coupled receptor (gpcr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CD97 antigen
Alternative name(s):
Leukocyte antigen CD97
CD_antigen: CD97
Cleaved into the following 2 chains:
Gene namesi
Name:CD97
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000123146.19.
HGNCiHGNC:1711. CD97.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 552ExtracellularSequence analysisAdd BLAST532
Transmembranei553 – 572Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini573 – 581CytoplasmicSequence analysis9
Transmembranei582 – 601Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini602 – 620ExtracellularSequence analysisAdd BLAST19
Transmembranei621 – 642Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini643 – 653CytoplasmicSequence analysisAdd BLAST11
Transmembranei654 – 674Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini675 – 691ExtracellularSequence analysisAdd BLAST17
Transmembranei692 – 712Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini713 – 739CytoplasmicSequence analysisAdd BLAST27
Transmembranei740 – 760Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini761 – 766ExtracellularSequence analysis6
Transmembranei767 – 789Helical; Name=7Sequence analysisAdd BLAST23
Topological domaini790 – 835CytoplasmicSequence analysisAdd BLAST46

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi976.
OpenTargetsiENSG00000123146.
PharmGKBiPA26248.

Polymorphism and mutation databases

BioMutaiCD97.
DMDMi90110013.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001286821 – 835CD97 antigenAdd BLAST815
ChainiPRO_000029623521 – 530CD97 antigen subunit alphaBy similarityAdd BLAST510
ChainiPRO_0000296236531 – 835CD97 antigen subunit betaBy similarityAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 36PROSITE-ProRule annotation
Disulfide bondi30 ↔ 42PROSITE-ProRule annotation
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi44 ↔ 62PROSITE-ProRule annotation
Disulfide bondi68 ↔ 82PROSITE-ProRule annotation
Disulfide bondi76 ↔ 91PROSITE-ProRule annotation
Disulfide bondi93 ↔ 114PROSITE-ProRule annotation
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi120 ↔ 133PROSITE-ProRule annotation
Disulfide bondi127 ↔ 142PROSITE-ProRule annotation
Disulfide bondi144 ↔ 158PROSITE-ProRule annotation
Disulfide bondi164 ↔ 177PROSITE-ProRule annotation
Disulfide bondi171 ↔ 186PROSITE-ProRule annotation
Disulfide bondi188 ↔ 207PROSITE-ProRule annotation
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi213 ↔ 226PROSITE-ProRule annotation
Disulfide bondi220 ↔ 235PROSITE-ProRule annotation
Disulfide bondi237 ↔ 256PROSITE-ProRule annotation
Glycosylationi371N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi406N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi413N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi453N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi520N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei815PhosphoserineCombined sources1
Modified residuei816PhosphothreonineCombined sources1
Modified residuei818PhosphoserineCombined sources1
Modified residuei825PhosphothreonineCombined sources1
Modified residuei831PhosphoserineCombined sources1
Modified residuei833PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei530 – 531CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP48960.
MaxQBiP48960.
PaxDbiP48960.
PeptideAtlasiP48960.
PRIDEiP48960.
TopDownProteomicsiP48960-2. [P48960-2]

PTM databases

iPTMnetiP48960.
PhosphoSitePlusiP48960.
SwissPalmiP48960.

Expressioni

Tissue specificityi

Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid.

Inductioni

Rapid up-regulation during lymphocyte activation.

Gene expression databases

BgeeiENSG00000123146.
CleanExiHS_CD97.
ExpressionAtlasiP48960. baseline and differential.
GenevisibleiP48960. HS.

Organism-specific databases

HPAiHPA013707.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) interacts with chondroitin sulfate.2 Publications

Protein-protein interaction databases

BioGridi107414. 59 interactors.
DIPiDIP-52292N.
IntActiP48960. 11 interactors.
MINTiMINT-4531069.
STRINGi9606.ENSP00000242786.

Structurei

3D structure databases

ProteinModelPortaliP48960.
SMRiP48960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 63EGF-like 1PROSITE-ProRule annotationAdd BLAST42
Domaini64 – 115EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST52
Domaini116 – 159EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST44
Domaini160 – 208EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST49
Domaini209 – 257EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST49
Domaini492 – 542GPSPROSITE-ProRule annotationAdd BLAST51

Domaini

The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain.

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOVERGENiHBG048917.
InParanoidiP48960.
KOiK08446.
OMAiREEYRKW.
OrthoDBiEOG091G063N.
PhylomeDBiP48960.
TreeFamiTF316380.

Family and domain databases

InterProiView protein in InterPro
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR003056. GPCR_2_CD97.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_.
PfamiView protein in Pfam
PF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 4 hits.
PF01825. GPS. 1 hit.
PRINTSiPR01278. CD97PROTEIN.
PR00249. GPCRSECRETIN.
SMARTiView protein in SMART
SM00181. EGF. 5 hits.
SM00179. EGF_CA. 4 hits.
SM00303. GPS. 1 hit.
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiView protein in PROSITE
PS00010. ASX_HYDROXYL. 4 hits.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 4 hits.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48960-1) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,3,4,5)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGRVFLAFC VWLTLPGAET QDSRGCARWC PQNSSCVNAT ACRCNPGFSS
60 70 80 90 100
FSEIITTPTE TCDDINECAT PSKVSCGKFS DCWNTEGSYD CVCSPGYEPV
110 120 130 140 150
SGAKTFKNES ENTCQDVDEC QQNPRLCKSY GTCVNTLGSY TCQCLPGFKF
160 170 180 190 200
IPEDPKVCTD VNECTSGQNP CHSSTHCLNN VGSYQCRCRP GWQPIPGSPN
210 220 230 240 250
GPNNTVCEDV DECSSGQHQC DSSTVCFNTV GSYSCRCRPG WKPRHGIPNN
260 270 280 290 300
QKDTVCEDMT FSTWTPPPGV HSQTLSRFFD KVQDLGRDSK TSSAEVTIQN
310 320 330 340 350
VIKLVDELME APGDVEALAP PVRHLIATQL LSNLEDIMRI LAKSLPKGPF
360 370 380 390 400
TYISPSNTEL TLMIQERGDK NVTMGQSSAR MKLNWAVAAG AEDPGPAVAG
410 420 430 440 450
ILSIQNMTTL LANASLNLHS KKQAELEEIY ESSIRGVQLR RLSAVNSIFL
460 470 480 490 500
SHNNTKELNS PILFAFSHLE SSDGEAGRDP PAKDVMPGPR QELLCAFWKS
510 520 530 540 550
DSDRGGHWAT EGCQVLGSKN GSTTCQCSHL SSFAILMAHY DVEDWKLTLI
560 570 580 590 600
TRVGLALSLF CLLLCILTFL LVRPIQGSRT TIHLHLCICL FVGSTIFLAG
610 620 630 640 650
IENEGGQVGL RCRLVAGLLH YCFLAAFCWM SLEGLELYFL VVRVFQGQGL
660 670 680 690 700
STRWLCLIGY GVPLLIVGVS AAIYSKGYGR PRYCWLDFEQ GFLWSFLGPV
710 720 730 740 750
TFIILCNAVI FVTTVWKLTQ KFSEINPDMK KLKKARALTI TAIAQLFLLG
760 770 780 790 800
CTWVFGLFIF DDRSLVLTYV FTILNCLQGA FLYLLHCLLN KKVREEYRKW
810 820 830
ACLVAGGSKY SEFTSTTSGT GHNQTRALRA SESGI
Length:835
Mass (Da):91,869
Last modified:March 21, 2006 - v4
Checksum:i06DDDE9178BC494B
GO
Isoform 2 (identifier: P48960-2) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,5)

The sequence of this isoform differs from the canonical sequence as follows:
     116-208: Missing.

Show »
Length:742
Mass (Da):81,743
Checksum:iC98F20493CA3E482
GO
Isoform 3 (identifier: P48960-3) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,3,5)

The sequence of this isoform differs from the canonical sequence as follows:
     160-208: Missing.

Show »
Length:786
Mass (Da):86,628
Checksum:i03982263B73EFA2A
GO

Sequence cautioni

The sequence AAC27673 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC06178 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103A → T in AAB36682 (PubMed:8955192).Curated1
Sequence conflicti512G → V (PubMed:7636245).Curated1
Sequence conflicti512G → V (PubMed:8786105).Curated1
Sequence conflicti534A → T (PubMed:7636245).Curated1
Sequence conflicti534A → T (PubMed:8786105).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017760367R → Q. Corresponds to variant dbSNP:rs2230748Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009411116 – 208Missing in isoform 2. 4 PublicationsAdd BLAST93
Alternative sequenceiVSP_009412160 – 208Missing in isoform 3. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84700 mRNA. Translation: CAA59173.1.
X94630
, X94631, X94632, X94633, Z99830, Z99831, X94634, X94635, X94636, X94637, X94638, X94639, X94640, X94641, X94642, X94643, X94644, X94645, X94646, X94647 Genomic DNA. Translation: CAA64333.1.
U76764 mRNA. Translation: AAB36682.1.
AB065966 Genomic DNA. Translation: BAC06178.1. Sequence problems.
AC005327 Genomic DNA. Translation: AAC27673.1. Sequence problems.
AK292159 mRNA. Translation: BAF84848.1.
AK314697 mRNA. Translation: BAG37246.1.
CH471106 Genomic DNA. Translation: EAW84412.1.
CH471106 Genomic DNA. Translation: EAW84413.1.
BC026690 mRNA. Translation: AAH26690.1.
CCDSiCCDS32929.1. [P48960-1]
CCDS32930.1. [P48960-3]
CCDS32931.1. [P48960-2]
PIRiI37225.
RefSeqiNP_001020331.1. NM_001025160.2. [P48960-3]
NP_001775.2. NM_001784.4. [P48960-2]
NP_510966.1. NM_078481.3. [P48960-1]
XP_016883036.1. XM_017027547.1. [P48960-1]
UniGeneiHs.466039.
Hs.531619.

Genome annotation databases

EnsembliENST00000242786; ENSP00000242786; ENSG00000123146. [P48960-1]
ENST00000357355; ENSP00000349918; ENSG00000123146. [P48960-3]
ENST00000358600; ENSP00000351413; ENSG00000123146. [P48960-2]
GeneIDi976.
KEGGihsa:976.
UCSCiuc002myl.4. human. [P48960-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCD97_HUMAN
AccessioniPrimary (citable) accession number: P48960
Secondary accession number(s): A8K7Z4
, B2RBJ9, O00718, O76101, Q8NG72, Q8TBQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 21, 2006
Last modified: September 27, 2017
This is version 183 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. SIMILARITY comments
    Index of protein domains and families