P48846 (CHIS_NOCSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitosanase
EC=3.2.1.132

Gene names

Name:

csn

Organism

Nocardioides sp. (strain N106)

Taxonomic identifier

47919 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
Catalytic activity	Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 46 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitosanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 278	237	Chitosanase		PRO_0000012208
Sites
	Active site	63	1	Proton donor By similarity
	Active site	81	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48846 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E76AA20065FAB4FF	FASTA	278	29,987
	10 20 30 40 50 60 MRLKHPTARL ALAALLVAVP RSVAAAGTVH AAPAPAGATR LAAVGLDDPH KKDIAMQLVS 70 80 90 100 110 120 SAENSSLDWK SQYKYIEDIK DGRGYTAGII GFCSGTGDML DLVADYTDLK PGNILAKYLP 130 140 150 160 170 180 ALRKVNGTES HAGLASAFEK DWATAAKDSV FQQAQNDERD RSYFNPAVNQ AKASLRALGQ 190 200 210 220 230 240 FAYYDAIVMH GPGDSSDSFG GIRKAAMKKA KTPAQGRDEA TYLKAFLAAR KTVMLKEEAH 250 260 270 SDTSRVDTEQ TVFLNAKNFD LNPPLKWKVY GDSYAINS

References

[1]	"A new chitosanase gene from a Nocardioides sp. is a third member of glycosyl hydrolase family 46." Masson J.Y., Boucher I., Neugebauer W.A., Ramotar D., Brzezinski R. Microbiology 141:2629-2635(1995) [PubMed: 7582023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L40408 Genomic DNA. Translation: AAA63405.1.
3D structure databases
ProteinModelPortal	P48846.
SMR	P48846. Positions 42-278.
ModBase	Search...
Protein family/group databases
CAZy	GH46. Glycoside Hydrolase Family 46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000400. Glyco_hydro_46. IPR023099. Glyco_hydro_46_N. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.386.10. Glyco_hydro_46_N. 1 hit.
Pfam	PF01374. Glyco_hydro_46. 1 hit. [Graphical view]
PIRSF	PIRSF036551. Chitosanase. 1 hit.
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS60000. CHITOSANASE_46_80. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIS_NOCSP

Accession

Primary (citable) accession number: P48846

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 5, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program