Reviewed, UniProtKB/Swiss-Prot P48841 (GANA_PSEFL)

Names and origin

Protein names

Recommended name:
    Arabinogalactan endo-1,4-beta-galactosidase
    EC=3.2.1.89
Alternative name(s):
    Endo-1,4-beta-galactanase
      Short name=Galactanase

Gene names

Name:	galA
Synonyms:	ganA

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-galactosidic linkages in arabinogalactans.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Induction	By galactan. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 53 family.
biophysicochemical properties	Kinetic parameters: KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius) pH dependence: Optimum pH is 7.5. Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	arabinogalactan endo-1,4-beta-galactosidase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW glucosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 376	359	Arabinogalactan endo-1,4-beta-galactosidase		PRO_0000012220
Sites
	Active site	161	1	Proton donor
	Active site	270	1	Nucleophile
	Metal binding	281	1	Calcium By similarity
	Metal binding	285	1	Calcium By similarity
Experimental info
	Mutagenesis	161	1	E → A: Partial loss of activity. Ref.1
	Mutagenesis	270	1	E → A: Complete loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48841-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BEC4F7243C6CAE10	FASTA	376	42,315
	10 20 30 40 50 60 MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG KKVDPFQLFA 70 80 90 100 110 120 DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT LLDFHYSDTW TDPEKQFIPK 130 140 150 160 170 180 AWAHITDTKE LAKALYDYTT DTLASLDQQQ LLPNLVQVGN ETNIEILQAE DTLVHGIPNW 190 200 210 220 230 240 QRNATLLNSG VNAVRDYSKK TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY 250 260 270 280 290 300 PQWSEYSLPQ LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP 310 320 330 340 350 360 RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF DATRKNNALP 370 AFLFFKADYQ ASAQAE

References

[1]

"Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues." Braithwaite K.L., Barna T., Spurway T.D., Charnock S.J., Black G.W., Hughes N., Lakey J.H., Virden R., Hazlewood G.P., Henrissat B., Gilbert H.J. Biochemistry 36:15489-15500(1997) [PubMed: 9398278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REACTION MECHANISM, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-161 AND GLU-270. Strain: Sp. Cellulosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91885 Genomic DNA. Translation: CAA62990.1.
3D structure databases
HSSP	HSSP built from PDB template 1FOB based on UniProtKB P48842.
ModBase	Search...
Protein family/group databases
CAZy	GH53. Glycoside Hydrolase Family 53.
Enzyme and pathway databases
BRENDA	3.2.1.89. 329.
Family and domain databases
InterPro	IPR011683. Glyco_hydro_53. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF07745. Glyco_hydro_53. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GANA_PSEFL

Accession

Primary (citable) accession number: P48841

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)