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P48841

- GANA_CELJU

UniProt

P48841 - GANA_CELJU

Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

ganB

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Kineticsi

    1. KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei161 – 1611Proton donor
    Active sitei270 – 2701Nucleophile
    Metal bindingi281 – 2811CalciumBy similarity
    Metal bindingi285 – 2851CalciumBy similarity

    GO - Molecular functioni

    1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
    2. glucosidase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-497-MONOMER.

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
    Alternative name(s):
    Endo-1,4-beta-galactanase
    Short name:
    Galactanase
    Gene namesi
    Name:ganB
    Synonyms:gal53A-2, galA, ganA
    Ordered Locus Names:CJA_0497
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi161 – 1611E → A: Partial loss of activity. 1 Publication
    Mutagenesisi270 – 2701E → A: Complete loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 376359Arabinogalactan endo-beta-1,4-galactanasePRO_0000012220Add
    BLAST

    Expressioni

    Inductioni

    By galactan.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_0497.

    Structurei

    3D structure databases

    ProteinModelPortaliP48841.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 53 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000118034.
    KOiK01224.
    OMAiVINTFAT.
    OrthoDBiEOG6H4K5M.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48841-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG    50
    KKVDPFQLFA DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT 100
    LLDFHYSDTW TDPEKQFIPK AWAHITDTKE LAKALYDYTT DTLASLDQQQ 150
    LLPNLVQVGN ETNIEILQAE DTLVHGIPNW QRNATLLNSG VNAVRDYSKK 200
    TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY PQWSEYSLPQ 250
    LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP 300
    RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF 350
    DATRKNNALP AFLFFKADYQ ASAQAE 376
    Length:376
    Mass (Da):42,315
    Last modified:February 1, 1996 - v1
    Checksum:iBEC4F7243C6CAE10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1.
    CP000934 Genomic DNA. Translation: ACE83887.1.
    RefSeqiYP_001981020.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
    GeneIDi6413839.
    KEGGicja:CJA_0497.
    PATRICi21324375. VBICelJap122165_0507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1 .
    CP000934 Genomic DNA. Translation: ACE83887.1 .
    RefSeqi YP_001981020.1. NC_010995.1.

    3D structure databases

    ProteinModelPortali P48841.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498211.CJA_0497.

    Protein family/group databases

    CAZyi GH53. Glycoside Hydrolase Family 53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE83887 ; ACE83887 ; CJA_0497 .
    GeneIDi 6413839.
    KEGGi cja:CJA_0497.
    PATRICi 21324375. VBICelJap122165_0507.

    Phylogenomic databases

    HOGENOMi HOG000118034.
    KOi K01224.
    OMAi VINTFAT.
    OrthoDBi EOG6H4K5M.

    Enzyme and pathway databases

    BioCyci CJAP498211:GHIT-497-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF07745. Glyco_hydro_53. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues."
      Braithwaite K.L., Barna T., Spurway T.D., Charnock S.J., Black G.W., Hughes N., Lakey J.H., Virden R., Hazlewood G.P., Henrissat B., Gilbert H.J.
      Biochemistry 36:15489-15500(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REACTION MECHANISM, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-161 AND GLU-270.
    2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.

    Entry informationi

    Entry nameiGANA_CELJU
    AccessioniPrimary (citable) accession number: P48841
    Secondary accession number(s): B3PIY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3