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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

ganB

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Kineticsi

  1. KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei161 – 1611Proton donor
    Active sitei270 – 2701Nucleophile
    Metal bindingi281 – 2811CalciumBy similarity
    Metal bindingi285 – 2851CalciumBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-497-MONOMER.

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
    Alternative name(s):
    Endo-1,4-beta-galactanase
    Short name:
    Galactanase
    Gene namesi
    Name:ganB
    Synonyms:gal53A-2, galA, ganA
    Ordered Locus Names:CJA_0497
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi161 – 1611E → A: Partial loss of activity. 1 Publication
    Mutagenesisi270 – 2701E → A: Complete loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 376359Arabinogalactan endo-beta-1,4-galactanasePRO_0000012220Add
    BLAST

    Expressioni

    Inductioni

    By galactan.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_0497.

    Structurei

    3D structure databases

    ProteinModelPortaliP48841.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 53 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000118034.
    KOiK01224.
    OMAiPQIMIHL.
    OrthoDBiEOG6H4K5M.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48841-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG
    60 70 80 90 100
    KKVDPFQLFA DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT
    110 120 130 140 150
    LLDFHYSDTW TDPEKQFIPK AWAHITDTKE LAKALYDYTT DTLASLDQQQ
    160 170 180 190 200
    LLPNLVQVGN ETNIEILQAE DTLVHGIPNW QRNATLLNSG VNAVRDYSKK
    210 220 230 240 250
    TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY PQWSEYSLPQ
    260 270 280 290 300
    LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP
    310 320 330 340 350
    RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF
    360 370
    DATRKNNALP AFLFFKADYQ ASAQAE
    Length:376
    Mass (Da):42,315
    Last modified:February 1, 1996 - v1
    Checksum:iBEC4F7243C6CAE10
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1.
    CP000934 Genomic DNA. Translation: ACE83887.1.
    RefSeqiWP_012486177.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
    KEGGicja:CJA_0497.
    PATRICi21324375. VBICelJap122165_0507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1.
    CP000934 Genomic DNA. Translation: ACE83887.1.
    RefSeqiWP_012486177.1. NC_010995.1.

    3D structure databases

    ProteinModelPortaliP48841.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi498211.CJA_0497.

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
    KEGGicja:CJA_0497.
    PATRICi21324375. VBICelJap122165_0507.

    Phylogenomic databases

    HOGENOMiHOG000118034.
    KOiK01224.
    OMAiPQIMIHL.
    OrthoDBiEOG6H4K5M.

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-497-MONOMER.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues."
      Braithwaite K.L., Barna T., Spurway T.D., Charnock S.J., Black G.W., Hughes N., Lakey J.H., Virden R., Hazlewood G.P., Henrissat B., Gilbert H.J.
      Biochemistry 36:15489-15500(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REACTION MECHANISM, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-161 AND GLU-270.
    2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.

    Entry informationi

    Entry nameiGANA_CELJU
    AccessioniPrimary (citable) accession number: P48841
    Secondary accession number(s): B3PIY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: July 22, 2015
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.