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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

ganB

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Kineticsi

  1. KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei161Proton donor1
    Active sitei270Nucleophile1
    Metal bindingi281CalciumBy similarity1
    Metal bindingi285CalciumBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
    Alternative name(s):
    Endo-1,4-beta-galactanase
    Short name:
    Galactanase
    Gene namesi
    Name:ganB
    Synonyms:gal53A-2, galA, ganA
    Ordered Locus Names:CJA_0497
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCellvibrionalesCellvibrionaceaeCellvibrio
    Proteomesi
    • UP000001036 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi161E → A: Partial loss of activity. 1 Publication1
    Mutagenesisi270E → A: Complete loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 17Sequence analysisAdd BLAST17
    ChainiPRO_000001222018 – 376Arabinogalactan endo-beta-1,4-galactanaseAdd BLAST359

    Proteomic databases

    PRIDEiP48841.

    Expressioni

    Inductioni

    By galactan.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_0497.

    Structurei

    3D structure databases

    ProteinModelPortaliP48841.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 53 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4106FKC. Bacteria.
    COG3867. LUCA.
    HOGENOMiHOG000118034.
    KOiK01224.
    OMAiVINTFAT.
    OrthoDBiPOG091H0X9F.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48841-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG
    60 70 80 90 100
    KKVDPFQLFA DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT
    110 120 130 140 150
    LLDFHYSDTW TDPEKQFIPK AWAHITDTKE LAKALYDYTT DTLASLDQQQ
    160 170 180 190 200
    LLPNLVQVGN ETNIEILQAE DTLVHGIPNW QRNATLLNSG VNAVRDYSKK
    210 220 230 240 250
    TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY PQWSEYSLPQ
    260 270 280 290 300
    LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP
    310 320 330 340 350
    RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF
    360 370
    DATRKNNALP AFLFFKADYQ ASAQAE
    Length:376
    Mass (Da):42,315
    Last modified:February 1, 1996 - v1
    Checksum:iBEC4F7243C6CAE10
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1.
    CP000934 Genomic DNA. Translation: ACE83887.1.
    RefSeqiWP_012486177.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
    KEGGicja:CJA_0497.
    PATRICi21324375. VBICelJap122165_0507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X91885 Genomic DNA. Translation: CAA62990.1.
    CP000934 Genomic DNA. Translation: ACE83887.1.
    RefSeqiWP_012486177.1. NC_010995.1.

    3D structure databases

    ProteinModelPortaliP48841.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi498211.CJA_0497.

    Protein family/group databases

    CAZyiGH53. Glycoside Hydrolase Family 53.

    Proteomic databases

    PRIDEiP48841.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
    KEGGicja:CJA_0497.
    PATRICi21324375. VBICelJap122165_0507.

    Phylogenomic databases

    eggNOGiENOG4106FKC. Bacteria.
    COG3867. LUCA.
    HOGENOMiHOG000118034.
    KOiK01224.
    OMAiVINTFAT.
    OrthoDBiPOG091H0X9F.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR011683. Glyco_hydro_53.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07745. Glyco_hydro_53. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGANA_CELJU
    AccessioniPrimary (citable) accession number: P48841
    Secondary accession number(s): B3PIY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: November 2, 2016
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.