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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

ganB

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Kineticsi

  1. KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Proton donor
Active sitei270 – 2701Nucleophile
Metal bindingi281 – 2811CalciumBy similarity
Metal bindingi285 – 2851CalciumBy similarity

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-497-MONOMER.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase
Short name:
Galactanase
Gene namesi
Name:ganB
Synonyms:gal53A-2, galA, ganA
Ordered Locus Names:CJA_0497
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
ProteomesiUP000001036 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1611E → A: Partial loss of activity. 1 Publication
Mutagenesisi270 – 2701E → A: Complete loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 376359Arabinogalactan endo-beta-1,4-galactanasePRO_0000012220Add
BLAST

Expressioni

Inductioni

By galactan.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_0497.

Structurei

3D structure databases

ProteinModelPortaliP48841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 53 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000118034.
KOiK01224.
OMAiVINTFAT.
OrthoDBiEOG6H4K5M.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG
60 70 80 90 100
KKVDPFQLFA DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT
110 120 130 140 150
LLDFHYSDTW TDPEKQFIPK AWAHITDTKE LAKALYDYTT DTLASLDQQQ
160 170 180 190 200
LLPNLVQVGN ETNIEILQAE DTLVHGIPNW QRNATLLNSG VNAVRDYSKK
210 220 230 240 250
TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY PQWSEYSLPQ
260 270 280 290 300
LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP
310 320 330 340 350
RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF
360 370
DATRKNNALP AFLFFKADYQ ASAQAE
Length:376
Mass (Da):42,315
Last modified:February 1, 1996 - v1
Checksum:iBEC4F7243C6CAE10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91885 Genomic DNA. Translation: CAA62990.1.
CP000934 Genomic DNA. Translation: ACE83887.1.
RefSeqiYP_001981020.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
KEGGicja:CJA_0497.
PATRICi21324375. VBICelJap122165_0507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91885 Genomic DNA. Translation: CAA62990.1.
CP000934 Genomic DNA. Translation: ACE83887.1.
RefSeqiYP_001981020.1. NC_010995.1.

3D structure databases

ProteinModelPortaliP48841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498211.CJA_0497.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
KEGGicja:CJA_0497.
PATRICi21324375. VBICelJap122165_0507.

Phylogenomic databases

HOGENOMiHOG000118034.
KOiK01224.
OMAiVINTFAT.
OrthoDBiEOG6H4K5M.

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-497-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues."
    Braithwaite K.L., Barna T., Spurway T.D., Charnock S.J., Black G.W., Hughes N., Lakey J.H., Virden R., Hazlewood G.P., Henrissat B., Gilbert H.J.
    Biochemistry 36:15489-15500(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REACTION MECHANISM, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-161 AND GLU-270.
  2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.

Entry informationi

Entry nameiGANA_CELJU
AccessioniPrimary (citable) accession number: P48841
Secondary accession number(s): B3PIY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.