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P48841

- GANA_CELJU

UniProt

P48841 - GANA_CELJU

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Protein
Arabinogalactan endo-beta-1,4-galactanase
Gene
ganB, gal53A-2, galA, ganA, CJA_0497
Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Kineticsi

  1. KM=2.3 mM for 2,4-dinitrophenyl-beta-galactobioside (at pH 7 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.5.

Temperature dependencei

Optimum temperature is 60 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Proton donor
Active sitei270 – 2701Nucleophile
Metal bindingi281 – 2811Calcium By similarity
Metal bindingi285 – 2851Calcium By similarity

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciCJAP498211:GHIT-497-MONOMER.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase
Short name:
Galactanase
Gene namesi
Name:ganB
Synonyms:gal53A-2, galA, ganA
Ordered Locus Names:CJA_0497
OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Taxonomic identifieri498211 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
ProteomesiUP000001036: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1611E → A: Partial loss of activity. 1 Publication
Mutagenesisi270 – 2701E → A: Complete loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Chaini18 – 376359Arabinogalactan endo-beta-1,4-galactanase
PRO_0000012220Add
BLAST

Expressioni

Inductioni

By galactan.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi498211.CJA_0497.

Structurei

3D structure databases

ProteinModelPortaliP48841.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000118034.
KOiK01224.
OMAiVINTFAT.
OrthoDBiEOG6H4K5M.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48841-1 [UniParc]FASTAAdd to Basket

« Hide

MKKKILAATA ILLAAIANTG VADNTPFYVG ADLSYVNEME SCGATYRDQG    50
KKVDPFQLFA DKGADLVRVR LWHNATWTKY SDLKDVSKTL KRAKNAGMKT 100
LLDFHYSDTW TDPEKQFIPK AWAHITDTKE LAKALYDYTT DTLASLDQQQ 150
LLPNLVQVGN ETNIEILQAE DTLVHGIPNW QRNATLLNSG VNAVRDYSKK 200
TGKPIQVVLH IAQPENALWW FKQAKENGVI DYDVIGLSYY PQWSEYSLPQ 250
LPDAIAELQN TYHKPVMIVE TAYPWTLHNF DQAGNVLGEK AVQPEFPASP 300
RGQLTYLLTL TQLVKSAGGM GVIYWEPAWV STRCRTLWGK GSHWENASFF 350
DATRKNNALP AFLFFKADYQ ASAQAE 376
Length:376
Mass (Da):42,315
Last modified:February 1, 1996 - v1
Checksum:iBEC4F7243C6CAE10
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91885 Genomic DNA. Translation: CAA62990.1.
CP000934 Genomic DNA. Translation: ACE83887.1.
RefSeqiYP_001981020.1. NC_010995.1.

Genome annotation databases

EnsemblBacteriaiACE83887; ACE83887; CJA_0497.
GeneIDi6413839.
KEGGicja:CJA_0497.
PATRICi21324375. VBICelJap122165_0507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91885 Genomic DNA. Translation: CAA62990.1 .
CP000934 Genomic DNA. Translation: ACE83887.1 .
RefSeqi YP_001981020.1. NC_010995.1.

3D structure databases

ProteinModelPortali P48841.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498211.CJA_0497.

Protein family/group databases

CAZyi GH53. Glycoside Hydrolase Family 53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE83887 ; ACE83887 ; CJA_0497 .
GeneIDi 6413839.
KEGGi cja:CJA_0497.
PATRICi 21324375. VBICelJap122165_0507.

Phylogenomic databases

HOGENOMi HOG000118034.
KOi K01224.
OMAi VINTFAT.
OrthoDBi EOG6H4K5M.

Enzyme and pathway databases

BioCyci CJAP498211:GHIT-497-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF07745. Glyco_hydro_53. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues."
    Braithwaite K.L., Barna T., Spurway T.D., Charnock S.J., Black G.W., Hughes N., Lakey J.H., Virden R., Hazlewood G.P., Henrissat B., Gilbert H.J.
    Biochemistry 36:15489-15500(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REACTION MECHANISM, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-161 AND GLU-270.
  2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
    DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
    J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ueda107.

Entry informationi

Entry nameiGANA_CELJU
AccessioniPrimary (citable) accession number: P48841
Secondary accession number(s): B3PIY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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