Reviewed, UniProtKB/Swiss-Prot P48839 (AGAA_VIBS7)

Names and origin

Protein names

Recommended name:
    Beta-agarase A
    EC=3.2.1.81
Alternative name(s):
    Agarase 0107

Gene names

Name:

agaA

Organism

Vibrio sp. (strain JT0107)

Taxonomic identifier

47913 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	995 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes agarose and also neoagarotetraose to yield neoagarobiose.
Catalytic activity	Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.
Sequence similarities	Belongs to the glycosyl hydrolase 50 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-agarase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 995	975	Beta-agarase A		PRO_0000012215
Regions
	Compositional bias	781 – 784	4	Poly-Ala
	Compositional bias	971 – 974	4	Poly-Gly

Sequences

	Sequence		Length	Mass (Da)
	P48839-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 437142F6C254124B	FASTA	995	107,276
	10 20 30 40 50 60 MKIKFLSAAI AASLALPLSA ATLVTSFEEA DYSSSENNAE FLEVSGDATS EVSTEQATDG 70 80 90 100 110 120 NQSIKASFDA AFKPMVVWNW ASWNWGAEDV MSVDVVNPND TDVTFAIKLI DSDILPDWVD 130 140 150 160 170 180 ESQTSLDYFT VSANTTQTFS FNLNGGNEFQ THGENFSKDK VIGVQFMLSE NDPQVLYFDN 190 200 210 220 230 240 IMVDGETVTP PPSDGAVNTQ TAPVATLAQI EDFETIPDYL RPDGGVNVST TTEIVTKGAA 250 260 270 280 290 300 AMAAEFTAGW NGLVFAGTWN WAELGEHTAV AVDVSNNSDS NIWLYSRIED VNSQGETATR 310 320 330 340 350 360 GVLVKAGESK TIYTSLNDNP SLLTQDERVS ALGLRDIPAD PMSAQNGWGD FVALDKSQIT 370 380 390 400 410 420 AIRYFIGELA SGETSQTLVF DNMRVIKDLN HESAYAEMTD AMGQNNLVTY AGKVASKEEL 430 440 450 460 470 480 AKLSDPEMAA LGELTNRNMY GGNPDSSPAT DCVLATPASF NACKDADGNW QLVDPAGNAF 490 500 510 520 530 540 FSTGVDNIRL QDTYTMTGVS SDAESESALR QSMFTEIPSD YVNENYGPVH SGPVSQGQAV 550 560 570 580 590 600 SFYANNLITR HASEDVWRDI TVKRMKDWGF NTLGNWTDPA LYANGDVPYV ANGWSTSGAD 610 620 630 640 650 660 RLPVKQIGSG YWGPLPDPWD ANFATNAATM AAEIKAQVEG NEEYLVGIFV DNEMSWGNVT 670 680 690 700 710 720 DVEGSRYAQT LAVFNTDGTD ATTSPAKNSF IWFLENQRYT GGIADLNAAW GTDYASWDAT 730 740 750 760 770 780 SPAQELAYVA GMEADMQFLA WQFAFQYFNT VNTALKAELP NHLYLGSRFA DWGRTPDVVS 790 800 810 820 830 840 AAAAVVDVMS YNIYKDSIAA ADWDADALSQ IEAIDKPVII GEFHFGALDS GSFAEGVVNA 850 860 870 880 890 900 TSQQDRADKM VSFYESVNAH KNFVGAHWFQ YIDSPLTGRA WDGENYNVGF VSNTDTPYTL 910 920 930 940 950 960 MTDAAREFNC GMYGTDCSSL SNATEAASRA GELYTGTNIG VSHSGPEAPD PGEPVDPPID 970 980 990 PPTPPTGGVT GGGGSAGWLS LLGLAGVFLL RRRKV

References

[1]	"Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107." Sugano Y., Matsumoto T., Kodama H., Noma M. Appl. Environ. Microbiol. 59:3750-3756(1993) [PubMed: 8285681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D14721 Genomic DNA. Translation: BAA03541.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH50. Glycoside Hydrolase Family 50.
Family and domain databases
InterPro	IPR020008. Gammaproteobac_enz_TM-dom_C. [Graphical view]
TIGRFAMs	TIGR03501. Gamma_C_targ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGAA_VIBS7

Accession

Primary (citable) accession number: P48839

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)