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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Processed zona pellucida sperm-binding protein 3 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 378ExtracellularSequence analysisAdd BLAST360
Transmembranei379 – 399HelicalSequence analysisAdd BLAST21
Topological domaini400 – 415CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 18By similarityAdd BLAST›18
ChainiPRO_000004171919 – 341Zona pellucida sperm-binding protein 3Add BLAST323
ChainiPRO_000030457419 – ?Processed zona pellucida sperm-binding protein 3
PropeptideiPRO_0000041720342 – 415Removed in mature formBy similarityAdd BLAST74

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Glycosylationi28O-linked (GalNAc...)By similarity1
Disulfide bondi42 ↔ 134By similarity
Disulfide bondi74 ↔ 93By similarity
Glycosylationi119N-linked (GlcNAc...)By similarity1
Glycosylationi141N-linked (GlcNAc...)By similarity1
Glycosylationi150O-linked (GalNAc...)By similarity1
Glycosylationi156O-linked (GalNAc...)By similarity1
Glycosylationi157O-linked (GalNAc...)By similarity1
Glycosylationi174N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi211 ↔ 276By similarity
Disulfide bondi233 ↔ 294By similarity
Glycosylationi266N-linked (GlcNAc...)By similarity1
Glycosylationi297N-linked (GlcNAc...)By similarity1

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.By similarity
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Oocytes (at protein level).1 Publication

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013539.

Structurei

3D structure databases

ProteinModelPortaliP48833.
SMRiP48833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 301ZPPROSITE-ProRule annotationAdd BLAST261

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9V. Eukaryota.
ENOG410YXE1. LUCA.
HOGENOMiHOG000220813.
HOVERGENiHBG007985.
InParanoidiP48833.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
YGLFVCLLLW GGSELCCPQP LWFWQGGTRQ PAPSVTPVVV ECLEARLVVT
60 70 80 90 100
VSRDLFGTGK LIQEADLSLG PEGCEPQAST DAVVRFEVGL HECGNSVQVT
110 120 130 140 150
DDSLVYSSFL LHDPRPAGNL SILRTNRAEV PIECRYPRQG NVSSRAILPT
160 170 180 190 200
WVPFWTTVLS EERLVFSLRL MEENWSREKM SPTFHLGDTA HLQAEVRTGS
210 220 230 240 250
HPPLLLFVDR CVATPTRDQS GSPYHTIVDL HGCLVDGLSD GASKFKAPRP
260 270 280 290 300
KPDVLQFMVA VFHFANDSRH TVYITCHLRV IPAQQAPDRL NKACSFNQSS
310 320 330 340 350
SSWAPVEGSA DICECCGNGD CDLIAGSPMN QNHAARSSLR SRRHVTEEAD
360 370 380 390 400
VTVGPLIFLG KAGDPAGTEG LASAAQATLV LGLRMATIVF LAVAAVVLGL
410
TRGRHAASHP RSASQ
Length:415
Mass (Da):44,987
Last modified:February 1, 1996 - v1
Checksum:i77396CF1BAA3F5CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05782 mRNA. Translation: AAA74392.1.
PIRiS70401.
UniGeneiOcu.2033.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05782 mRNA. Translation: AAA74392.1.
PIRiS70401.
UniGeneiOcu.2033.

3D structure databases

ProteinModelPortaliP48833.
SMRiP48833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410II9V. Eukaryota.
ENOG410YXE1. LUCA.
HOGENOMiHOG000220813.
HOVERGENiHBG007985.
InParanoidiP48833.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZP3_RABIT
AccessioniPrimary (citable) accession number: P48833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.