Reviewed, UniProtKB/Swiss-Prot P48833 (ZP3_RABIT)

Names and origin

Protein names

Recommended name:
    Zona pellucida sperm-binding protein 3
Alternative name(s):
    Zona pellucida glycoprotein ZP3
    Zona pellucida protein C
    Sperm receptor
Cleaved into the following chain:
    1- Recommended name:
            Processed zona pellucida sperm-binding protein 3

Gene names

Name:	ZP3
Synonyms:	ZPC

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	415 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated By similarity. O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPC subfamily. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 18	›18	By similarity
	Chain	19 – 341	323	Zona pellucida sperm-binding protein 3		PRO_0000041719
	Chain	19 – ?		Processed zona pellucida sperm-binding protein 3		PRO_0000304574
	Propeptide	342 – 415	74	Removed in mature form By similarity		PRO_0000041720
Regions
	Topological domain	19 – 378	360	Extracellular Potential
	Transmembrane	379 – 399	21	Potential
	Topological domain	400 – 415	16	Cytoplasmic Potential
	Domain	41 – 301	261	ZP
Amino acid modifications
	Modified residue	19	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	28	1	O-linked (GalNAc...) By similarity
	Glycosylation	119	1	N-linked (GlcNAc...) By similarity
	Glycosylation	141	1	N-linked (GlcNAc...) By similarity
	Glycosylation	150	1	O-linked (GalNAc...) By similarity
	Glycosylation	156	1	O-linked (GalNAc...) By similarity
	Glycosylation	157	1	O-linked (GalNAc...) By similarity
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) By similarity
	Glycosylation	297	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	42 ↔ 134		By similarity
	Disulfide bond	74 ↔ 93		By similarity
	Disulfide bond	211 ↔ 276		By similarity
	Disulfide bond	233 ↔ 294		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P48833-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 77396CF1BAA3F5CB	FASTA	415	44,987
	10 20 30 40 50 60 YGLFVCLLLW GGSELCCPQP LWFWQGGTRQ PAPSVTPVVV ECLEARLVVT VSRDLFGTGK 70 80 90 100 110 120 LIQEADLSLG PEGCEPQAST DAVVRFEVGL HECGNSVQVT DDSLVYSSFL LHDPRPAGNL 130 140 150 160 170 180 SILRTNRAEV PIECRYPRQG NVSSRAILPT WVPFWTTVLS EERLVFSLRL MEENWSREKM 190 200 210 220 230 240 SPTFHLGDTA HLQAEVRTGS HPPLLLFVDR CVATPTRDQS GSPYHTIVDL HGCLVDGLSD 250 260 270 280 290 300 GASKFKAPRP KPDVLQFMVA VFHFANDSRH TVYITCHLRV IPAQQAPDRL NKACSFNQSS 310 320 330 340 350 360 SSWAPVEGSA DICECCGNGD CDLIAGSPMN QNHAARSSLR SRRHVTEEAD VTVGPLIFLG 370 380 390 400 410 KAGDPAGTEG LASAAQATLV LGLRMATIVF LAVAAVVLGL TRGRHAASHP RSASQ

References

[1]

"Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families." Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G. DNA Seq. 4:361-393(1994) [PubMed: 7841460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05782 mRNA. Translation: AAA74392.1.
PIR	S70401.
UniGene	Ocu.2033
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48833.
Genome annotation databases
Ensembl	ENSOCUT00000015760; ENSOCUP00000013539; ENSOCUG00000015762; Oryctolagus cuniculus. [Genome view]
Phylogenomic databases
HOVERGEN	P48833.
Family and domain databases
InterPro	IPR001507. Endoglin/CD105. IPR017977. Endoglin/CD105_CS. IPR017976. Endoglin/CD105_subgr. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZP3_RABIT

Accession

Primary (citable) accession number: P48833

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	October 13, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)