SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P48832

- ZP3_FELCA

UniProt

P48832 - ZP3_FELCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zona pellucida sperm-binding protein 3

Gene
ZP3, ZPC
Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB
  2. manganese ion transmembrane transporter activity Source: UniProtKB
  3. signal transducer activity Source: UniProtKB
  4. store-operated calcium channel activity Source: Ensembl

GO - Biological processi

  1. binding of sperm to zona pellucida Source: UniProtKB
  2. blastocyst formation Source: UniProtKB
  3. egg coat formation Source: UniProtKB
  4. humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
  5. intracellular protein transport Source: UniProtKB
  6. intracellular signal transduction Source: UniProtKB
  7. manganese ion transport Source: UniProtKB
  8. negative regulation of binding of sperm to zona pellucida Source: UniProtKB
  9. negative regulation of transcription, DNA-templated Source: UniProtKB
  10. oocyte development Source: UniProtKB
  11. phosphatidylinositol-mediated signaling Source: UniProtKB
  12. positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
  13. positive regulation of acrosome reaction Source: UniProtKB
  14. positive regulation of antral ovarian follicle growth Source: UniProtKB
  15. positive regulation of calcium ion import Source: UniProtKB
  16. positive regulation of humoral immune response Source: UniProtKB
  17. positive regulation of inflammatory response Source: UniProtKB
  18. positive regulation of interferon-gamma production Source: UniProtKB
  19. positive regulation of interleukin-4 production Source: UniProtKB
  20. positive regulation of leukocyte migration Source: UniProtKB
  21. positive regulation of ovarian follicle development Source: UniProtKB
  22. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  23. positive regulation of protein kinase activity Source: UniProtKB
  24. positive regulation of protein kinase B signaling Source: UniProtKB
  25. positive regulation of T cell proliferation Source: UniProtKB
  26. positive regulation of transcription, DNA-templated Source: UniProtKB
  27. positive regulation of type IV hypersensitivity Source: UniProtKB
  28. protein kinase C signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Chromosome E3

Subcellular locationi

Chain Processed zona pellucida sperm-binding protein 3 : Secretedextracellular spaceextracellular matrix
Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 383361Extracellular Reviewed predictionAdd
BLAST
Transmembranei384 – 40421Helical; Reviewed predictionAdd
BLAST
Topological domaini405 – 42420Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. extracellular matrix Source: UniProtKB
  4. extracellular space Source: UniProtKB
  5. Golgi apparatus Source: UniProtKB
  6. integral component of membrane Source: UniProtKB-KW
  7. multivesicular body Source: UniProtKB
  8. outer acrosomal membrane Source: UniProtKB
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. plasma membrane Source: UniProtKB
  11. proteinaceous extracellular matrix Source: UniProtKB-SubCell
  12. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 By similarityAdd
BLAST
Chaini23 – 348326Zona pellucida sperm-binding protein 3PRO_0000041707Add
BLAST
Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304568
Propeptidei349 – 42476Removed in mature form By similarityPRO_0000041708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 138 By similarity
Glycosylationi52 – 521N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi76 ↔ 97 By similarity
Glycosylationi123 – 1231N-linked (GlcNAc...) By similarity
Glycosylationi145 – 1451N-linked (GlcNAc...) By similarity
Glycosylationi154 – 1541O-linked (GalNAc...) By similarity
Glycosylationi160 – 1601O-linked (GalNAc...) By similarity
Glycosylationi161 – 1611O-linked (GalNAc...) By similarity
Disulfide bondi215 ↔ 280 By similarity
Disulfide bondi237 ↔ 298 By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated By similarity.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP48832.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 305263ZPAdd
BLAST

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Contains 1 ZP domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063482.
HOVERGENiHBG007985.
OMAiLMEEDWG.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48832-1 [UniParc]FASTAAdd to Basket

« Hide

MGLSYGLFIC FLLWAGTGLC YPPTTTEDKT HPSLPSSPSV VVECRHAWLV    50
VNVSKNLFGT GRLVRPADLT LGPENCEPLI SGDSDDTVRF EVELHKCGNS 100
VQVTEDALVY STFLLHNPRP MGNLSILRTN RAEVPIECRY PRHSNVSSEA 150
ILPTWVPFRT TMLSEEKLAF SLRLMEEDWG SEKQSPTFQL GDLAHLQAEV 200
HTGRHIPLRL FVDYCVATLT PDQNASPHHT IVDFHGCLVD GLSDASSAFK 250
APRPRPETLQ FTVDTFHFAN DPRNMIYITC HLKVTPASRV PDQLNKACSF 300
IKSSNRWFPV EGPADICNCC NKGSCGLQGR SWRLSHLDRP WHKMASRNRR 350
HVTEEADITV GPLIFLGKAA DRGVEGSTSP HTSVMVGIGL ATVLSLTLAT 400
IVLGLARRHH TASRPMICPV SASQ 424
Length:424
Mass (Da):46,854
Last modified:February 1, 1996 - v1
Checksum:iCFC62F35F9AAFC0D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721G → W in BAA08096. 1 Publication
Sequence conflicti264 – 2641D → Y in BAA08096. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05778 mRNA. Translation: AAA74390.1.
D45068 mRNA. Translation: BAA08096.1.
PIRiS70399.
RefSeqiNP_001009330.1. NM_001009330.2.

Genome annotation databases

EnsembliENSFCAT00000013478; ENSFCAP00000012496; ENSFCAG00000013474.
GeneIDi493925.
KEGGifca:493925.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05778 mRNA. Translation: AAA74390.1 .
D45068 mRNA. Translation: BAA08096.1 .
PIRi S70399.
RefSeqi NP_001009330.1. NM_001009330.2.

3D structure databases

ProteinModelPortali P48832.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSFCAT00000013478 ; ENSFCAP00000012496 ; ENSFCAG00000013474 .
GeneIDi 493925.
KEGGi fca:493925.

Organism-specific databases

CTDi 7784.

Phylogenomic databases

GeneTreei ENSGT00530000063482.
HOVERGENi HBG007985.
OMAi LMEEDWG.

Family and domain databases

InterProi IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view ]
Pfami PF00100. Zona_pellucida. 1 hit.
[Graphical view ]
PRINTSi PR00023. ZPELLUCIDA.
SMARTi SM00241. ZP. 1 hit.
[Graphical view ]
PROSITEi PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families."
    Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G.
    DNA Seq. 4:361-393(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. Okazaki Y., Sugimoto M.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiZP3_FELCA
AccessioniPrimary (citable) accession number: P48832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi