Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P48832

- ZP3_FELCA

UniProt

P48832 - ZP3_FELCA

Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

    GO - Molecular functioni

    1. carbohydrate binding Source: UniProtKB
    2. manganese ion transmembrane transporter activity Source: UniProtKB
    3. signal transducer activity Source: UniProtKB
    4. store-operated calcium channel activity Source: Ensembl

    GO - Biological processi

    1. binding of sperm to zona pellucida Source: UniProtKB
    2. blastocyst formation Source: UniProtKB
    3. egg coat formation Source: UniProtKB
    4. humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
    5. intracellular protein transport Source: UniProtKB
    6. intracellular signal transduction Source: UniProtKB
    7. manganese ion transport Source: UniProtKB
    8. negative regulation of binding of sperm to zona pellucida Source: UniProtKB
    9. negative regulation of transcription, DNA-templated Source: UniProtKB
    10. oocyte development Source: UniProtKB
    11. phosphatidylinositol-mediated signaling Source: UniProtKB
    12. positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
    13. positive regulation of acrosome reaction Source: UniProtKB
    14. positive regulation of antral ovarian follicle growth Source: UniProtKB
    15. positive regulation of calcium ion import Source: UniProtKB
    16. positive regulation of humoral immune response Source: UniProtKB
    17. positive regulation of inflammatory response Source: UniProtKB
    18. positive regulation of interferon-gamma production Source: UniProtKB
    19. positive regulation of interleukin-4 production Source: UniProtKB
    20. positive regulation of leukocyte migration Source: UniProtKB
    21. positive regulation of ovarian follicle development Source: UniProtKB
    22. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
    23. positive regulation of protein kinase activity Source: UniProtKB
    24. positive regulation of protein kinase B signaling Source: UniProtKB
    25. positive regulation of T cell proliferation Source: UniProtKB
    26. positive regulation of transcription, DNA-templated Source: UniProtKB
    27. positive regulation of type IV hypersensitivity Source: UniProtKB
    28. protein kinase C signaling Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Fertilization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zona pellucida sperm-binding protein 3
    Alternative name(s):
    Sperm receptor
    Zona pellucida glycoprotein 3
    Short name:
    Zp-3
    Zona pellucida protein C
    Cleaved into the following chain:
    Gene namesi
    Name:ZP3
    Synonyms:ZPC
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Chromosome E3

    Subcellular locationi

    Chain Processed zona pellucida sperm-binding protein 3 : Secretedextracellular spaceextracellular matrix
    Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. extracellular matrix Source: UniProtKB
    4. extracellular space Source: UniProtKB
    5. Golgi apparatus Source: UniProtKB
    6. integral component of membrane Source: UniProtKB-KW
    7. multivesicular body Source: UniProtKB
    8. outer acrosomal membrane Source: UniProtKB
    9. perinuclear region of cytoplasm Source: UniProtKB
    10. plasma membrane Source: UniProtKB
    11. proteinaceous extracellular matrix Source: UniProtKB-SubCell
    12. secretory granule Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Extracellular matrix, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 348326Zona pellucida sperm-binding protein 3PRO_0000041707Add
    BLAST
    Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304568
    Propeptidei349 – 42476Removed in mature formBy similarityPRO_0000041708Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 138By similarity
    Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi76 ↔ 97By similarity
    Glycosylationi123 – 1231N-linked (GlcNAc...)By similarity
    Glycosylationi145 – 1451N-linked (GlcNAc...)By similarity
    Glycosylationi154 – 1541O-linked (GalNAc...)By similarity
    Glycosylationi160 – 1601O-linked (GalNAc...)By similarity
    Glycosylationi161 – 1611O-linked (GalNAc...)By similarity
    Disulfide bondi215 ↔ 280By similarity
    Disulfide bondi237 ↔ 298By similarity

    Post-translational modificationi

    Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    N-glycosylated.By similarity
    O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Oocytes.

    Interactioni

    Subunit structurei

    Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP48832.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 383361ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini405 – 42420CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 305263ZPPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

    Sequence similaritiesi

    Belongs to the ZP domain family. ZPC subfamily.Curated
    Contains 1 ZP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00530000063482.
    HOVERGENiHBG007985.
    OMAiLMEEDWG.

    Family and domain databases

    InterProiIPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view]
    PfamiPF00100. Zona_pellucida. 1 hit.
    [Graphical view]
    PRINTSiPR00023. ZPELLUCIDA.
    SMARTiSM00241. ZP. 1 hit.
    [Graphical view]
    PROSITEiPS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48832-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLSYGLFIC FLLWAGTGLC YPPTTTEDKT HPSLPSSPSV VVECRHAWLV    50
    VNVSKNLFGT GRLVRPADLT LGPENCEPLI SGDSDDTVRF EVELHKCGNS 100
    VQVTEDALVY STFLLHNPRP MGNLSILRTN RAEVPIECRY PRHSNVSSEA 150
    ILPTWVPFRT TMLSEEKLAF SLRLMEEDWG SEKQSPTFQL GDLAHLQAEV 200
    HTGRHIPLRL FVDYCVATLT PDQNASPHHT IVDFHGCLVD GLSDASSAFK 250
    APRPRPETLQ FTVDTFHFAN DPRNMIYITC HLKVTPASRV PDQLNKACSF 300
    IKSSNRWFPV EGPADICNCC NKGSCGLQGR SWRLSHLDRP WHKMASRNRR 350
    HVTEEADITV GPLIFLGKAA DRGVEGSTSP HTSVMVGIGL ATVLSLTLAT 400
    IVLGLARRHH TASRPMICPV SASQ 424
    Length:424
    Mass (Da):46,854
    Last modified:February 1, 1996 - v1
    Checksum:iCFC62F35F9AAFC0D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721G → W in BAA08096. 1 PublicationCurated
    Sequence conflicti264 – 2641D → Y in BAA08096. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05778 mRNA. Translation: AAA74390.1.
    D45068 mRNA. Translation: BAA08096.1.
    PIRiS70399.
    RefSeqiNP_001009330.1. NM_001009330.2.

    Genome annotation databases

    EnsembliENSFCAT00000013478; ENSFCAP00000012496; ENSFCAG00000013474.
    GeneIDi493925.
    KEGGifca:493925.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Molecular chastity - Issue 93 of April 2008

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05778 mRNA. Translation: AAA74390.1 .
    D45068 mRNA. Translation: BAA08096.1 .
    PIRi S70399.
    RefSeqi NP_001009330.1. NM_001009330.2.

    3D structure databases

    ProteinModelPortali P48832.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSFCAT00000013478 ; ENSFCAP00000012496 ; ENSFCAG00000013474 .
    GeneIDi 493925.
    KEGGi fca:493925.

    Organism-specific databases

    CTDi 7784.

    Phylogenomic databases

    GeneTreei ENSGT00530000063482.
    HOVERGENi HBG007985.
    OMAi LMEEDWG.

    Family and domain databases

    InterProi IPR001507. ZP_dom.
    IPR017977. ZP_dom_CS.
    [Graphical view ]
    Pfami PF00100. Zona_pellucida. 1 hit.
    [Graphical view ]
    PRINTSi PR00023. ZPELLUCIDA.
    SMARTi SM00241. ZP. 1 hit.
    [Graphical view ]
    PROSITEi PS00682. ZP_1. 1 hit.
    PS51034. ZP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families."
      Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G.
      DNA Seq. 4:361-393(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.
    2. Okazaki Y., Sugimoto M.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiZP3_FELCA
    AccessioniPrimary (citable) accession number: P48832
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3