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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712 Componenti: Chromosome E3

Subcellular locationi

Processed zona pellucida sperm-binding protein 3 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 383361ExtracellularSequence AnalysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence AnalysisAdd
BLAST
Topological domaini405 – 42420CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 348326Zona pellucida sperm-binding protein 3PRO_0000041707Add
BLAST
Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304568
Propeptidei349 – 42476Removed in mature formBy similarityPRO_0000041708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 138By similarity
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi76 ↔ 97By similarity
Glycosylationi123 – 1231N-linked (GlcNAc...)By similarity
Glycosylationi145 – 1451N-linked (GlcNAc...)By similarity
Glycosylationi154 – 1541O-linked (GalNAc...)By similarity
Glycosylationi160 – 1601O-linked (GalNAc...)By similarity
Glycosylationi161 – 1611O-linked (GalNAc...)By similarity
Disulfide bondi215 ↔ 280By similarity
Disulfide bondi237 ↔ 298By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.By similarity
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000012496.

Structurei

3D structure databases

ProteinModelPortaliP48832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 305263ZPPROSITE-ProRule annotationAdd
BLAST

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063482.
HOVERGENiHBG007985.
InParanoidiP48832.
OMAiNRRHVTE.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSYGLFIC FLLWAGTGLC YPPTTTEDKT HPSLPSSPSV VVECRHAWLV
60 70 80 90 100
VNVSKNLFGT GRLVRPADLT LGPENCEPLI SGDSDDTVRF EVELHKCGNS
110 120 130 140 150
VQVTEDALVY STFLLHNPRP MGNLSILRTN RAEVPIECRY PRHSNVSSEA
160 170 180 190 200
ILPTWVPFRT TMLSEEKLAF SLRLMEEDWG SEKQSPTFQL GDLAHLQAEV
210 220 230 240 250
HTGRHIPLRL FVDYCVATLT PDQNASPHHT IVDFHGCLVD GLSDASSAFK
260 270 280 290 300
APRPRPETLQ FTVDTFHFAN DPRNMIYITC HLKVTPASRV PDQLNKACSF
310 320 330 340 350
IKSSNRWFPV EGPADICNCC NKGSCGLQGR SWRLSHLDRP WHKMASRNRR
360 370 380 390 400
HVTEEADITV GPLIFLGKAA DRGVEGSTSP HTSVMVGIGL ATVLSLTLAT
410 420
IVLGLARRHH TASRPMICPV SASQ
Length:424
Mass (Da):46,854
Last modified:February 1, 1996 - v1
Checksum:iCFC62F35F9AAFC0D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721G → W in BAA08096 (Ref. 2) Curated
Sequence conflicti264 – 2641D → Y in BAA08096 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05778 mRNA. Translation: AAA74390.1.
D45068 mRNA. Translation: BAA08096.1.
PIRiS70399.
RefSeqiNP_001009330.1. NM_001009330.2.

Genome annotation databases

EnsembliENSFCAT00000013478; ENSFCAP00000012496; ENSFCAG00000013474.
GeneIDi493925.
KEGGifca:493925.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05778 mRNA. Translation: AAA74390.1.
D45068 mRNA. Translation: BAA08096.1.
PIRiS70399.
RefSeqiNP_001009330.1. NM_001009330.2.

3D structure databases

ProteinModelPortaliP48832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000012496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSFCAT00000013478; ENSFCAP00000012496; ENSFCAG00000013474.
GeneIDi493925.
KEGGifca:493925.

Organism-specific databases

CTDi7784.

Phylogenomic databases

GeneTreeiENSGT00530000063482.
HOVERGENiHBG007985.
InParanoidiP48832.
OMAiNRRHVTE.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families."
    Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G.
    DNA Seq. 4:361-393(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. Okazaki Y., Sugimoto M.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiZP3_FELCA
AccessioniPrimary (citable) accession number: P48832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.