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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processFertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Chromosome E3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 383ExtracellularSequence analysisAdd BLAST361
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 424CytoplasmicSequence analysisAdd BLAST20

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000004170723 – 348Zona pellucida sperm-binding protein 3Add BLAST326
ChainiPRO_000030456823 – ?Processed zona pellucida sperm-binding protein 3
PropeptideiPRO_0000041708349 – 424Removed in mature formBy similarityAdd BLAST76

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 138By similarity
Glycosylationi52N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi76 ↔ 97By similarity
Glycosylationi123N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi145N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi154O-linked (GalNAc...) threonineBy similarity1
Glycosylationi160O-linked (GalNAc...) threonineBy similarity1
Glycosylationi161O-linked (GalNAc...) threonineBy similarity1
Disulfide bondi215 ↔ 280By similarity
Disulfide bondi237 ↔ 298By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.By similarity
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Oocytes.

Gene expression databases

BgeeiENSFCAG00000013474.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000012496.

Structurei

3D structure databases

ProteinModelPortaliP48832.
SMRiP48832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 305ZPPROSITE-ProRule annotationAdd BLAST263

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9V. Eukaryota.
ENOG410YXE1. LUCA.
GeneTreeiENSGT00530000063482.
HOVERGENiHBG007985.
InParanoidiP48832.
KOiK19928.
OMAiNRRHVTE.
OrthoDBiEOG091G0AQ6.

Family and domain databases

InterProiView protein in InterPro
IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
PfamiView protein in Pfam
PF00100. Zona_pellucida. 1 hit.
PRINTSiPR00023. ZPELLUCIDA.
SMARTiView protein in SMART
SM00241. ZP. 1 hit.
PROSITEiView protein in PROSITE
PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSYGLFIC FLLWAGTGLC YPPTTTEDKT HPSLPSSPSV VVECRHAWLV
60 70 80 90 100
VNVSKNLFGT GRLVRPADLT LGPENCEPLI SGDSDDTVRF EVELHKCGNS
110 120 130 140 150
VQVTEDALVY STFLLHNPRP MGNLSILRTN RAEVPIECRY PRHSNVSSEA
160 170 180 190 200
ILPTWVPFRT TMLSEEKLAF SLRLMEEDWG SEKQSPTFQL GDLAHLQAEV
210 220 230 240 250
HTGRHIPLRL FVDYCVATLT PDQNASPHHT IVDFHGCLVD GLSDASSAFK
260 270 280 290 300
APRPRPETLQ FTVDTFHFAN DPRNMIYITC HLKVTPASRV PDQLNKACSF
310 320 330 340 350
IKSSNRWFPV EGPADICNCC NKGSCGLQGR SWRLSHLDRP WHKMASRNRR
360 370 380 390 400
HVTEEADITV GPLIFLGKAA DRGVEGSTSP HTSVMVGIGL ATVLSLTLAT
410 420
IVLGLARRHH TASRPMICPV SASQ
Length:424
Mass (Da):46,854
Last modified:February 1, 1996 - v1
Checksum:iCFC62F35F9AAFC0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72G → W in BAA08096 (Ref. 2) Curated1
Sequence conflicti264D → Y in BAA08096 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05778 mRNA. Translation: AAA74390.1.
D45068 mRNA. Translation: BAA08096.1.
PIRiS70399.
RefSeqiNP_001009330.1. NM_001009330.2.

Genome annotation databases

EnsembliENSFCAT00000013478; ENSFCAP00000012496; ENSFCAG00000013474.
GeneIDi493925.
KEGGifca:493925.

Similar proteinsi

Entry informationi

Entry nameiZP3_FELCA
AccessioniPrimary (citable) accession number: P48832
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families