Reviewed, UniProtKB/Swiss-Prot P48831 (ZP3_CANFA)

Names and origin

Protein names

Recommended name:
    Zona pellucida sperm-binding protein 3
Alternative name(s):
    Zona pellucida glycoprotein ZP3
    Zona pellucida protein C
    Sperm receptor
Cleaved into the following chain:
    1- Recommended name:
            Processed zona pellucida sperm-binding protein 3

Gene names

Name:	ZP3
Synonyms:	ZPC

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated By similarity. O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPC subfamily. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 350	328	Zona pellucida sperm-binding protein 3		PRO_0000041705
	Chain	23 – ?		Processed zona pellucida sperm-binding protein 3		PRO_0000304567
	Propeptide	351 – 426	76	Removed in mature form By similarity		PRO_0000041706
Regions
	Topological domain	23 – 385	363	Extracellular Potential
	Transmembrane	386 – 406	21	Potential
	Topological domain	407 – 426	20	Cytoplasmic Potential
	Domain	43 – 305	263	ZP
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	123	1	N-linked (GlcNAc...) By similarity
	Glycosylation	145	1	N-linked (GlcNAc...) By similarity
	Glycosylation	154	1	O-linked (GalNAc...) By similarity
	Glycosylation	160	1	O-linked (GalNAc...) By similarity
	Glycosylation	161	1	O-linked (GalNAc...) By similarity
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 138		By similarity
	Disulfide bond	76 ↔ 97		By similarity
	Disulfide bond	215 ↔ 280		By similarity
	Disulfide bond	237 ↔ 298		By similarity
Experimental info
	Sequence conflict	227	1	L → P in BAA08098. Ref.2
	Sequence conflict	307	1	W → S in BAA08098. Ref.2
	Sequence conflict	343	1	K → R in BAA08098. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48831-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BE5825A949DCA172	FASTA	426	47,368
	10 20 30 40 50 60 MGLSYGIFIC FLLLGGMELC CPQTIWPTET YYPLTSRPPV MVDCLESQLV VTVSKDLFGT 70 80 90 100 110 120 GKLIRPADLT LGPENCEPLV SMDTDDVVRF EVGLHECGSR VQVTDNALVY STFLIHSPRP 130 140 150 160 170 180 AGNLSILRTN RAEVPIECHY PRHSNVSSQA ILPTWVPFRT TMLFEEKLVF SLRLMEEDWG 190 200 210 220 230 240 SEKQSPTFQL GDIAHLQAEV HTGSHMPLRL FVDHCVATLT PDRNAFLHHK IVDFHGCLVD 250 260 270 280 290 300 GLYNSSSAFK APRPRPETLQ FTVDVFHFAK DSRNTIYITC HLKVTPADRV PDQLNKACSF 310 320 330 340 350 360 IKSTKRWYPV EGSADICRCC NKGSCGLPGR SRRLSHLERG WRKSVSHTRN RRHVTEEAEI 370 380 390 400 410 420 TVGPLIFLGK ASDHGIEGST SPHTSVMLGL GLATVVSLTL ATIVLVLAKR HRTASHPVIC PASVSQ

References

[1]	"Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families." Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G. DNA Seq. 4:361-393(1994) [PubMed: 7841460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	Okazaki Y., Sugimoto M. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05780 mRNA. Translation: AAA74387.1. D45070 mRNA. Translation: BAA08098.1.
PIR	S70396.
RefSeq	NP_001003224.1.
UniGene	Cfa.3754
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48831.
Genome annotation databases
Ensembl	ENSCAFT00000021646; ENSCAFP00000020101; ENSCAFG00000013648; Canis familiaris. [Genome view]
GeneID	403894.
KEGG	cfa:403894.
Organism-specific databases
CTD	403894.
Phylogenomic databases
HOVERGEN	P48831.
InParanoid	P48831.
Family and domain databases
InterPro	IPR001507. Endoglin/CD105. IPR017977. Endoglin/CD105_CS. IPR017976. Endoglin/CD105_subgr. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZP3_CANFA

Accession

Primary (citable) accession number: P48831

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)