P48830 (ZP3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zona pellucida sperm-binding protein 3

Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name=Zp-3
Zona pellucida glycoprotein 3B
Short name=Zp-3B
Zona pellucida protein C

Cleaved into the following chain:

1. Processed zona pellucida sperm-binding protein 3

Gene names

Name:	ZP3
Synonyms:	ZPC

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated By similarity. O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPC subfamily. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	binding of sperm to zona pellucida Inferred from physical interaction. Source: UniProtKB blastocyst formation Inferred from sequence or structural similarity. Source: UniProtKB egg coat formation Inferred from sequence or structural similarity. Source: UniProtKB humoral immune response mediated by circulating immunoglobulin Inferred from sequence or structural similarity. Source: UniProtKB intracellular protein transport Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB oocyte development Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol-mediated signaling Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of T cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of acrosomal vesicle exocytosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of acrosome reaction Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of antral ovarian follicle growth Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of calcium ion import Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of calcium ion transport via store-operated calcium channel activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of humoral immune response Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interferon-gamma production Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interleukin-4 production Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of leukocyte migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ovarian follicle development Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of phosphatidylinositol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of type IV hypersensitivity Inferred from sequence or structural similarity. Source: UniProtKB protein kinase C signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB extracellular space Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body Inferred from sequence or structural similarity. Source: UniProtKB outer acrosomal membrane Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion transmembrane transporter activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: UniProtKB receptor activity Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from sequence or structural similarity. Source: UniProtKB sugar binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 346	324	Zona pellucida sperm-binding protein 3		PRO_0000041701
	Chain	23 – ?		Processed zona pellucida sperm-binding protein 3		PRO_0000304565
	Propeptide	347 – 421	75	Removed in mature form By similarity		PRO_0000041702
Regions
	Topological domain	23 – 381	359	Extracellular Potential
	Transmembrane	382 – 402	21	Helical; Potential
	Topological domain	403 – 421	19	Cytoplasmic Potential
	Domain	44 – 306	263	ZP
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	124	1	N-linked (GlcNAc...) By similarity
	Glycosylation	146	1	N-linked (GlcNAc...) By similarity
	Glycosylation	155	1	O-linked (GalNAc...) By similarity
	Glycosylation	161	1	O-linked (GalNAc...) By similarity
	Glycosylation	162	1	O-linked (GalNAc...) By similarity
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	45 ↔ 139		By similarity
	Disulfide bond	77 ↔ 98		By similarity
	Disulfide bond	216 ↔ 281		By similarity
	Disulfide bond	238 ↔ 299		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48830 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 905C4722B7BA11DC	FASTA	421	46,545
	10 20 30 40 50 60 MGPCSRLFVC FLLWGSTELC SPQPFWDDET ERFRPSKPPA VMVECQEAQL VVTVDKDLFG 70 80 90 100 110 120 TGKLIRPADL TLGPDNCEPL ASADTDGVVR FAVGLHECGN ILQVTDNALV YSTFLLHNPR 130 140 150 160 170 180 PAGNLSILRT NRAEVPIECH YPRQGNVSSW AIQPTWVPFR TTVFSEEKLV FSLRLMEENW 190 200 210 220 230 240 SAEKMTPTFQ LGDRAHLQAQ VHTGSHVPLR LFVDHCVASL TPDWSTSPYH TIVDFHGCLV 250 260 270 280 290 300 DGLTDASSAF KAPRPRPEIL QFTVDVFRFA NDSRNMIYIT CHLKVTPVDR VPDQLNKACS 310 320 330 340 350 360 FSKSSNRWSP VEGPTDICRC CSKGRCGISG RSMRLSHREG RPVPRSRRHV TEEADVTVGP 370 380 390 400 410 420 LIFLRKMNDR GVEGPTSSPP LVMLGLGLAT VMTLTLAAIV LGLTGRLRAA SHPVCPVSAS Q

References

[1]

"Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families." Harris J.D., Hibler D.W., Fontenot G.K., Hsu K.T., Yurewicz E.C., Sacco A.G. DNA Seq. 4:361-393(1994) [PubMed: 7841460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05775 mRNA. Translation: AAA74385.1.
IPI	IPI00703432.
PIR	S70402.
RefSeq	NP_776399.1. NM_173974.2.
UniGene	Bt.511.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48830.
Proteomic databases
PRIDE	P48830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	280964.
KEGG	bta:280964.
Organism-specific databases
CTD	7784.
Phylogenomic databases
eggNOG	maNOG06569.
HOVERGEN	HBG007985.
InParanoid	P48830.
OrthoDB	EOG437RFK.
Family and domain databases
InterPro	IPR017977. Endoglin/CD105_CS. IPR017976. Endoglin/CD105_subgr. IPR001507. Zona_pellucida_Endoglin/CD105. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZP3_BOVIN

Accession

Primary (citable) accession number: P48830

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program