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Protein

Endochitinase 42

Gene

chit42

Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a morphogenetic role during apical growth, cell division and differentiation (cell wall morphogenesis). Acts also as an antifungal agent. Involved in the degradation and further assimilation of phytopathogenic fungi, namely mycoparasitism, the major mechanism accounting for the antagonistic activity against phytopathogenic fungi displayed by Trichoderma.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication

Kineticsi

  1. KM=0.3 mg/ml for colloidal chitin1 Publication

    Temperature dependencei

    Optimum temperature is 45-50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei171Proton donorPROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
    LigandChitin-binding

    Enzyme and pathway databases

    BRENDAi3.2.1.14. 6445.
    SABIO-RKiP48827.

    Protein family/group databases

    CAZyiGH18. Glycoside Hydrolase Family 18.
    mycoCLAPiCHI18D_TRIHA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endochitinase 42 (EC:3.2.1.14)
    Alternative name(s):
    42 kDa endochitinase
    Chitinase 42
    Gene namesi
    Name:chit42
    OrganismiTrichoderma harzianum (Hypocrea lixii)
    Taxonomic identifieri5544 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Biotechnological usei

    The antagonistic activity of Trichoderma harzianum is used for the control of several soil borne plant pathogenic fungi.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 22Sequence analysisAdd BLAST22
    PropeptideiPRO_000001193423 – 341 PublicationAdd BLAST12
    ChainiPRO_000001193535 – 423Endochitinase 42Add BLAST389

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi218N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Cleavage on pair of basic residues, Glycoprotein, Zymogen

    Expressioni

    Inductioni

    Specifically induced by chitin and is catabolite repressed.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP48827.
    SMRiP48827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.50.10. 1 hit.
    InterProiView protein in InterPro
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18_cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR017853. Glycoside_hydrolase_SF.
    PfamiView protein in Pfam
    PF00704. Glyco_hydro_18. 1 hit.
    SMARTiView protein in SMART
    SM00636. Glyco_18. 1 hit.
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    PROSITEiView protein in PROSITE
    PS01095. CHITINASE_18. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48827-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLSFLGKSVA LLAALQATLS SPKPGHRRAS VEKRANGYAN SVYFTNWGIY
    60 70 80 90 100
    DRNFQPADLV ASDVTHVIYS FMNLQADGTV ISGDTYADYE KHYADDSWND
    110 120 130 140 150
    VGTNAYGCVK QLFKVKKANR GLKVLLSIGG WTWSTNFPSA ASTDANRKNF
    160 170 180 190 200
    AKTAITFMKD WGFDGIDIDW EYPADATQAS NMILLLKEVR SQRDAYAAQY
    210 220 230 240 250
    APGYHFLLTI AAPAGKDNYS KLRLADLGQV LDYINLMAYD YAGSFSPLTG
    260 270 280 290 300
    HDANLFNNPS NPNATPFNTD SAVKDYINGG VPANKIVLGM PIYGRSFQNT
    310 320 330 340 350
    AGIGQTYNGV GSGSWEAGIW DYKALPKAGA TVQYDSVAKG YYSYNSATKE
    360 370 380 390 400
    LISFDTPDMI NTKVAYLKSL GLGGSMFWEA SADKKGADSV IGTSHRALGG
    410 420
    LDTTQNLLSY PNSKYDNIKN GLN
    Length:423
    Mass (Da):46,057
    Last modified:February 1, 1996 - v1
    Checksum:iDF90378EED1C30BE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    S78423 mRNA. Translation: AAB34355.1.
    PIRiS51369.

    Similar proteinsi

    Entry informationi

    Entry nameiCHI42_TRIHA
    AccessioniPrimary (citable) accession number: P48827
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: May 10, 2017
    This is version 91 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families