P48827 (CHI4_TRIHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
42 kDa endochitinase
EC=3.2.1.14

Gene names

Name:

chit42

Organism

Trichoderma harzianum (Hypocrea lixii)

Taxonomic identifier

5544 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Morphogenetic role during apical growth, cell division and differentiation (cell wall morphogenesis). Antifungal agent.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted.
Induction	Specifically induced by chitin and is catabolite repressed.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 34	12			PRO_0000011934
	Chain	35 – 423	389	42 kDa endochitinase		PRO_0000011935
Sites
	Active site	171	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	218	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48827 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DF90378EED1C30BE	FASTA	423	46,057
	10 20 30 40 50 60 MLSFLGKSVA LLAALQATLS SPKPGHRRAS VEKRANGYAN SVYFTNWGIY DRNFQPADLV 70 80 90 100 110 120 ASDVTHVIYS FMNLQADGTV ISGDTYADYE KHYADDSWND VGTNAYGCVK QLFKVKKANR 130 140 150 160 170 180 GLKVLLSIGG WTWSTNFPSA ASTDANRKNF AKTAITFMKD WGFDGIDIDW EYPADATQAS 190 200 210 220 230 240 NMILLLKEVR SQRDAYAAQY APGYHFLLTI AAPAGKDNYS KLRLADLGQV LDYINLMAYD 250 260 270 280 290 300 YAGSFSPLTG HDANLFNNPS NPNATPFNTD SAVKDYINGG VPANKIVLGM PIYGRSFQNT 310 320 330 340 350 360 AGIGQTYNGV GSGSWEAGIW DYKALPKAGA TVQYDSVAKG YYSYNSATKE LISFDTPDMI 370 380 390 400 410 420 NTKVAYLKSL GLGGSMFWEA SADKKGADSV IGTSHRALGG LDTTQNLLSY PNSKYDNIKN GLN

References

[1]	"Cloning and characterization of a chitinase (chit42) cDNA from the mycoparasitic fungus Trichoderma harzianum." Garcia I., Lora J.M., de la Cruz J., Benitez T., Llobell A., Pintor-Toro J.A. Curr. Genet. 27:83-89(1994) [PubMed: 7750151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-52; 93-107; 371-385 AND 397-414.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S78423 mRNA. Translation: AAB34355.1.
PIR	S51369.
3D structure databases
ProteinModelPortal	P48827.
SMR	P48827. Positions 35-423.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.14. 6445.
Family and domain databases
InterPro	IPR011583. Chitinase_II. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SMART	SM00636. Glyco_18. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI4_TRIHA

Accession

Primary (citable) accession number: P48827

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program