Reviewed, UniProtKB/Swiss-Prot P48823 (HEXA_PSEO7)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase A
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase
    Chitobiase

Gene names

Name:

cht60

Organism

Pseudoalteromonas piscicida

Taxonomic identifier

43662 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Most active towards p-nitrophenyl-N-acetyl-beta-D-glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5. Temperature dependence: Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 11	11	Ref.1
	Chain	12 – 598	587	Beta-hexosaminidase A		PRO_0000011784
Sites
	Active site	305	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48823-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5131B17B84DA9688	FASTA	598	64,539
	10 20 30 40 50 60 MSFITSAHAT AAQVPLTTSQ MLGQKLMLDF RYYCGESKKP SGDCRAAMTT LPPELSELIS 70 80 90 100 110 120 RYDIGGAILF AENVQNTAQI ISLTNALQSA AQQSKSQLPL FIAIDQEGGR VARINREQAT 130 140 150 160 170 180 SFTGNMSIGA TYPKQGDIYA TKVASAIGKE LNSLGINVNF APTVDVNSNP NNPVINVRSF 190 200 210 220 230 240 SENPTVVTKL GLAQVKAFEA AGVLSALKHF PGHGDTHVDS HTGLPRVDHD RDKINQQDLL 250 260 270 280 290 300 PFAEIIKASP PGMIMTAHIQ YPALDNSKVV NSQGESMIRP ATMSYQIMTQ LLRHELGYQG 310 320 330 340 350 360 VTVTDALDMA GISDFFNPVD ATIETFNAGV DIALMPIAIR NRADIKRFEQ YMAQLADALE 370 380 390 400 410 420 TNKLNQEQLS SSMARIAKLK TKLPQSSASL AIANSTLGNP SHRRLEAELA LAAITEVKND 430 440 450 460 470 480 GVLPLRDNAQ VVHLIMPDRQ KCFALEQALQ TYSKNSLTLS CTSLQAYDPD IAHDAIKQAD 490 500 510 520 530 540 MIIAAHASPP QSAVEIGGMD DVKKLREHGV ARNVQPAALK ALLQYGQQQG KKQLFISLRA 550 560 570 580 590 PYEISTFGPL SNAVLASYAY NVDVNHDKKV AGPAYTALAK VILGIAKAEG SLPVTVNH

References

[1]

"Gene sequence, purification and characterization of N-acetyl-beta-glucosaminidase from a marine bacterium, Alteromonas sp. strain O-7." Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Imada C., Okami Y., Inamori Y. Gene 146:111-115(1994) [PubMed: 8063094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 12-22. Strain: O-7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17399 Genomic DNA. Translation: BAA04223.1.
3D structure databases
SMR	P48823. Positions 49-337.
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Family and domain databases
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. IPR017853. Glyco_hydro_catalytic_core. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEXA_PSEO7

Accession

Primary (citable) accession number: P48823

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)