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P48823 (HEXA_PSEO7) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase A

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
Chitobiase
N-acetyl-beta-glucosaminidase
Gene names
Name:cht60
OrganismPseudoalteromonas piscicida
Taxonomic identifier43662 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Most active towards p-nitrophenyl-N-acetyl-beta-D-glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose.

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5.

Temperature dependence:

Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-N-acetylhexosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1111 Ref.1
Chain12 – 598587Beta-hexosaminidase A
PRO_0000011784

Sites

Active site3051 By similarity

Sequences

Sequence LengthMass (Da)Tools
P48823 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 5131B17B84DA9688

FASTA59864,539
        10         20         30         40         50         60 
MSFITSAHAT AAQVPLTTSQ MLGQKLMLDF RYYCGESKKP SGDCRAAMTT LPPELSELIS 

        70         80         90        100        110        120 
RYDIGGAILF AENVQNTAQI ISLTNALQSA AQQSKSQLPL FIAIDQEGGR VARINREQAT 

       130        140        150        160        170        180 
SFTGNMSIGA TYPKQGDIYA TKVASAIGKE LNSLGINVNF APTVDVNSNP NNPVINVRSF 

       190        200        210        220        230        240 
SENPTVVTKL GLAQVKAFEA AGVLSALKHF PGHGDTHVDS HTGLPRVDHD RDKINQQDLL 

       250        260        270        280        290        300 
PFAEIIKASP PGMIMTAHIQ YPALDNSKVV NSQGESMIRP ATMSYQIMTQ LLRHELGYQG 

       310        320        330        340        350        360 
VTVTDALDMA GISDFFNPVD ATIETFNAGV DIALMPIAIR NRADIKRFEQ YMAQLADALE 

       370        380        390        400        410        420 
TNKLNQEQLS SSMARIAKLK TKLPQSSASL AIANSTLGNP SHRRLEAELA LAAITEVKND 

       430        440        450        460        470        480 
GVLPLRDNAQ VVHLIMPDRQ KCFALEQALQ TYSKNSLTLS CTSLQAYDPD IAHDAIKQAD 

       490        500        510        520        530        540 
MIIAAHASPP QSAVEIGGMD DVKKLREHGV ARNVQPAALK ALLQYGQQQG KKQLFISLRA 

       550        560        570        580        590 
PYEISTFGPL SNAVLASYAY NVDVNHDKKV AGPAYTALAK VILGIAKAEG SLPVTVNH 

« Hide

References

[1]"Gene sequence, purification and characterization of N-acetyl-beta-glucosaminidase from a marine bacterium, Alteromonas sp. strain O-7."
Tsujibo H., Fujimoto K., Tanno H., Miyamoto K., Imada C., Okami Y., Inamori Y.
Gene 146:111-115(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 12-22.
Strain: O-7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17399 Genomic DNA. Translation: BAA04223.1.

3D structure databases

ProteinModelPortalP48823.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.300. 1 hit.
InterProIPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEXA_PSEO7
AccessionPrimary (citable) accession number: P48823
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries