Reviewed, UniProtKB/Swiss-Prot P48819 (VTNC_PIG)

Names and origin

Protein names

Recommended name:
    Vitronectin
Alternative name(s):
    Serum-spreading factor
      Short name=S-protein

Gene names

Name:

VTN

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway By similarity.
Subunit structure	Monomer. Interacts with SERPINE1/PAI1 By similarity.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Plasma.
Domain	The SMB domain mediates interaction with SERPINE1/PAI1 By similarity.
Post-translational modification	Sulfated on 2 tyrosine residues By similarity. N- and O-glycosylated By similarity. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Sequence similarities	Contains 4 hemopexin-like domains. Contains 1 SMB (somatomedin-B) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Heparin-binding
PTM	Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.2
	Chain	20 – 459	440	Vitronectin		PRO_0000036399
Regions
	Domain	20 – 63	44	SMB
	Domain	138 – 181	44	Hemopexin-like 1
	Domain	183 – 229	47	Hemopexin-like 2
	Domain	231 – 281	51	Hemopexin-like 3
	Domain	406 – 453	48	Hemopexin-like 4
	Motif	64 – 66	3	Cell attachment site Potential
Amino acid modifications
	Modified residue	75	1	Sulfotyrosine Potential
	Modified residue	78	1	Sulfotyrosine Potential
	Modified residue	80	1	Sulfotyrosine Potential
	Modified residue	148	1	Phosphoserine By similarity
	Modified residue	289	1	Phosphoserine By similarity
	Modified residue	398	1	Sulfotyrosine Potential
	Modified residue	401	1	Sulfotyrosine Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 40		Alternate By similarity
	Disulfide bond	24 ↔ 28		Alternate By similarity
	Disulfide bond	28 ↔ 58		Alternate By similarity
	Disulfide bond	38 ↔ 51		Alternate By similarity
	Disulfide bond	38 ↔ 40		Alternate By similarity
	Disulfide bond	44 ↔ 50		By similarity
	Disulfide bond	51 ↔ 58		Alternate By similarity
Experimental info
	Sequence conflict	44	1	C → L AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48819-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 630E134B0DC706BE	FASTA	459	52,572
	10 20 30 40 50 60 MAPLRPLLML ALLAWVALAD QESCKGRCTD GFIAERKCQC DELCSYYQSC CTDYVAECKP 70 80 90 100 110 120 QVTRGDVFLQ PDDEYRAYDY HEETRHNTSV QEEQRIPVLL AKTEETPVLK PEEEAPPPGP 130 140 150 160 170 180 QTDDLGVPEE ELCSGKPFDA FTNLKNGSVF AFRGLYCYEL DEKAVRPGYP KLIQDVWGIK 190 200 210 220 230 240 GPIDAAFTRI NCQGKTYLFK GSQYWRFDDG VLDPNYPREI SEGFKGIPDD VDAALALPAH 250 260 270 280 290 300 SYSGRERVYF FKGKQYWEYV FQQQPSREEC EGSSPSDVFA HFALMQRDSW EDIFRLLFWS 310 320 330 340 350 360 HSFGGAIEPR VISQDWLGLP EQVDAAMAGQ IYISGSALKP SQPKMTKSAR RSGKRYRSRR 370 380 390 400 410 420 GRGRGRGHSR SQKSHRQSRS TWLPWFSSEE TGPGGYNYDD YKMDWLVPAT CEPIQSVYFF 430 440 450 SGEEYYRVNL RTQRVDTVTP PYPRSIAQYW LGCPVPDQK

References

[1]	"Porcine vitronectin, the most compact form of single-chain vitronectin: the smallest molecular mass among vitronectins was ascribed to deletion and substitution of base pairs, and proteolytic trimming of the peptide." Yoneda A., Kojima K., Matsumoto I., Yamamoto K., Ogawa H. J. Biochem. 120:954-960(1996) [PubMed: 8982862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Vitronectin diversity in evolution but uniformity in ligand binding and size of the core polypeptide." Nakashima N., Miyazaki K., Ishikawa M., Yatohgo T., Ogawa H., Uchibori H., Matsumoto I., Seno N., Hayashi M. Biochim. Biophys. Acta 1120:1-10(1992) [PubMed: 1372829] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-44.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63145 mRNA. Translation: BAA09630.1.
PIR	JC5139.
RefSeq	NP_999269.1.
UniGene	Ssc.32202
3D structure databases
HSSP	HSSP built from PDB template 1OC0 based on UniProtKB P04004.
SMR	P48819. Positions 20-68.
ModBase	Search...
PTM databases
GlycoSuiteDB	P48819.
Genome annotation databases
GeneID	397192.
KEGG	ssc:397192.
Phylogenomic databases
HOVERGEN	P48819.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001212. Somatomedin_B. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 2 hits.
Pfam	PF00045. Hemopexin. 4 hits. PF01033. Somatomedin_B. 1 hit. [Graphical view]
SMART	SM00120. HX. 4 hits. SM00201. SO. 1 hit. [Graphical view]
PROSITE	PS00024. HEMOPEXIN. 1 hit. PS00524. SMB_1. 1 hit. PS50958. SMB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VTNC_PIG

Accession

Primary (citable) accession number: P48819

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)