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P48818 (ACADV_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
Short name=VLCAD
EC=1.3.99.-
Gene names
Name:ACADVL
Synonyms:VLCAD
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, myristoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.

Catalytic activity

Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.

Cofactor

FAD.

Pathway

Lipid metabolism; mitochondrial fatty acid beta-oxidation.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion inner membrane.

Miscellaneous

A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Mitochondrion By similarity
Chain41 – 655615Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
PRO_0000000514

Regions

Nucleotide binding214 – 22310FAD By similarity
Nucleotide binding249 – 2513FAD By similarity
Nucleotide binding435 – 4395FAD By similarity
Nucleotide binding464 – 4663FAD By similarity
Region41 – 482442Catalytic
Region338 – 3414Substrate binging By similarity
Region462 – 4632Substrate binding By similarity
Region483 – 51634Membrane-anchoring Probable

Sites

Active site4621Proton acceptor By similarity
Binding site2231Substrate; via carbonyl oxygen By similarity
Binding site3661FAD By similarity
Binding site4631Substrate; via amide nitrogen By similarity

Amino acid modifications

Modified residue2391N6-acetyllysine By similarity
Modified residue2761N6-acetyllysine By similarity
Modified residue3311N6-acetyllysine By similarity
Cross-link331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict21R → Q in AAA74051. Ref.1
Sequence conflict7 – 82AQ → TA in AAA74051. Ref.1
Sequence conflict101T → L in AAA74051. Ref.1
Sequence conflict131Q → T in AAA74051. Ref.1
Sequence conflict371R → P in AAA74051. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48818 [UniParc].

Last modified November 13, 2007. Version 3.
Checksum: 677FCBD01AD3DA8B

FASTA65570,649
        10         20         30         40         50         60 
MRAARMAQST GRQLLRLRGV SSWPGELLGQ PRPGPARRPY ASGVAQAAVD QSDSQPSEAS 

        70         80         90        100        110        120 
TREKRANSVS KSFAVGTFKG QLTTDQVFPY PSVLNEDQTQ FLKELVGPVT RFFEEVNDAA 

       130        140        150        160        170        180 
KNDMLERVEE TTMQGLKELG AFGLQVPNEL GGVGLCNTQY ARLVEIVGMY DLGVGIVLGA 

       190        200        210        220        230        240 
HQSIGFKGIL LFGTKAQKEK YLPKLASGET IAAFCLTEPS SGSDAASIRS SAVPSPCGKY 

       250        260        270        280        290        300 
YTLNGSKIWI SNGGLADIFT VFAKTPVTDT ATGAVKEKIT AFVVERSFGG VTHGPPEKKM 

       310        320        330        340        350        360 
GIKASNTAEV YFDGVRVPAE NVLGEVGGGF KVAMHILNNG RFGMAAALAG TMKGIIAKAV 

       370        380        390        400        410        420 
DHAANRTQFG EKIHNFGLIQ EKLARMAMLQ YVTESMAYMV SANMDQGSTD FQIEAAISKI 

       430        440        450        460        470        480 
FGSEAAWKVT DECIQIMGGM GFMKEPGVER VLRDLRIFRI FEGTNDILRL FVALQGCMDK 

       490        500        510        520        530        540 
GKELSGLGNA LKNPFGNAGL LLGEAGKQLR RRAGLGSGLS LSGIVHQELS RSGELAVQAL 

       550        560        570        580        590        600 
EQFATVVEAK LIKHKKDIIN EQFLLQRLAD SAIDLYAMVV VLSRASRSLS EGHPTAQHEK 

       610        620        630        640        650 
MLCDSWCIEA AARIRENMTA LQSDPQQQEL FRNFKSISKA LVERGGVVTS NPLGF

« Hide

References

« Hide 'large scale' references
[1]"The molecular cloning and the nucleotide sequence of cDNA encoding the precursor of the mitochondrial very-long-chain acyl-CoA dehydrogenase from bovine heart."
Zhang X., Liu W., Zhu H., Sun X.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Heart ventricle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U30817 mRNA. Translation: AAA74051.1.
BT030546 mRNA. Translation: ABQ12986.1.
BC103104 mRNA. Translation: AAI03105.1.
IPIIPI00696178.
RefSeqNP_776919.1. NM_174494.2.
UniGeneBt.48920.

3D structure databases

ProteinModelPortalP48818.
SMRP48818. Positions 70-655.
ModBaseSearch...

Protein-protein interaction databases

IntActP48818. 35 interactions.
STRINGP48818.

Proteomic databases

PRIDEP48818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000003999; ENSBTAP00000003999; ENSBTAG00000003072.
GeneID282130.
KEGGbta:282130.

Organism-specific databases

CTD37.

Phylogenomic databases

eggNOGmaNOG08365.
GeneTreeENSGT00590000082821.
HOVERGENHBG050448.
InParanoidP48818.
OMAKNDALEM.
OrthoDBEOG4P5K8R.

Family and domain databases

InterProIPR006089. Acyl-CoA_DH_CS.
IPR006092. Acyl-CoA_DH_N.
IPR006090. Acyl-CoA_Oxase/DH_1.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase.
[Graphical view]
Gene3DG3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit.
G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit.
G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 2 hits.
KOK09479.
PfamPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMSSF56645. AcylCoA_dehyd_NM. 1 hit.
SSF47203. AcylCoADH_C_like. 1 hit.
PROSITEPS00072. ACYL_COA_DH_1. 1 hit.
PS00073. ACYL_COA_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACADV_BOVIN
AccessionPrimary (citable) accession number: P48818
Secondary accession number(s): A5D9C7, Q3SZ66
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: January 25, 2012
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents