Reviewed, UniProtKB/Swiss-Prot P48818 (ACADV_BOVIN)

Names and origin

Protein names

Recommended name:
    Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
      Short name=VLCAD
    EC=1.3.99.-

Gene names

Name:	ACADVL
Synonyms:	VLCAD

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
Catalytic activity	Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF.
Cofactor	FAD.
Pathway	Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion By similarity
	Chain	41 – 655	615	Very long-chain specific acyl-CoA dehydrogenase, mitochondrial		PRO_0000000514
Regions
	Region	41 – 482	442	Catalytic
	Region	483 – 516	34	Membrane-anchoring Probable
Sites
	Active site	462	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	239	1	N6-acetyllysine By similarity
	Modified residue	276	1	N6-acetyllysine By similarity
	Cross-link	331		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Experimental info
	Sequence conflict	2	1	R → Q in AAA74051. Ref.1
	Sequence conflict	7 – 8	2	AQ → TA in AAA74051. Ref.1
	Sequence conflict	10	1	T → L in AAA74051. Ref.1
	Sequence conflict	13	1	Q → T in AAA74051. Ref.1
	Sequence conflict	37	1	R → P in AAA74051. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48818-1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: 677FCBD01AD3DA8B	FASTA	655	70,649
	10 20 30 40 50 60 MRAARMAQST GRQLLRLRGV SSWPGELLGQ PRPGPARRPY ASGVAQAAVD QSDSQPSEAS 70 80 90 100 110 120 TREKRANSVS KSFAVGTFKG QLTTDQVFPY PSVLNEDQTQ FLKELVGPVT RFFEEVNDAA 130 140 150 160 170 180 KNDMLERVEE TTMQGLKELG AFGLQVPNEL GGVGLCNTQY ARLVEIVGMY DLGVGIVLGA 190 200 210 220 230 240 HQSIGFKGIL LFGTKAQKEK YLPKLASGET IAAFCLTEPS SGSDAASIRS SAVPSPCGKY 250 260 270 280 290 300 YTLNGSKIWI SNGGLADIFT VFAKTPVTDT ATGAVKEKIT AFVVERSFGG VTHGPPEKKM 310 320 330 340 350 360 GIKASNTAEV YFDGVRVPAE NVLGEVGGGF KVAMHILNNG RFGMAAALAG TMKGIIAKAV 370 380 390 400 410 420 DHAANRTQFG EKIHNFGLIQ EKLARMAMLQ YVTESMAYMV SANMDQGSTD FQIEAAISKI 430 440 450 460 470 480 FGSEAAWKVT DECIQIMGGM GFMKEPGVER VLRDLRIFRI FEGTNDILRL FVALQGCMDK 490 500 510 520 530 540 GKELSGLGNA LKNPFGNAGL LLGEAGKQLR RRAGLGSGLS LSGIVHQELS RSGELAVQAL 550 560 570 580 590 600 EQFATVVEAK LIKHKKDIIN EQFLLQRLAD SAIDLYAMVV VLSRASRSLS EGHPTAQHEK 610 620 630 640 650 MLCDSWCIEA AARIRENMTA LQSDPQQQEL FRNFKSISKA LVERGGVVTS NPLGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The molecular cloning and the nucleotide sequence of cDNA encoding the precursor of the mitochondrial very-long-chain acyl-CoA dehydrogenase from bovine heart." Zhang X., Liu W., Zhu H., Sun X. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30817 mRNA. Translation: AAA74051.1. BT030546 mRNA. Translation: ABQ12986.1. BC103104 mRNA. Translation: AAI03105.1.
IPI	IPI00696178.
RefSeq	NP_776919.1.
UniGene	Bt.48920
3D structure databases
HSSP	HSSP built from PDB template 1BUC based on UniProtKB Q06319.
SMR	P48818. Positions 70-655.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000003072. Bos taurus. [Contig view]
GeneID	282130.
KEGG	bta:282130.
Phylogenomic databases
HOVERGEN	P48818.
OMA	P48818. KNDALEM.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACADV_BOVIN

Accession

Primary (citable) accession number: P48818
Secondary accession number(s): A5D9C7, Q3SZ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)