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Protein

High-affinity glutamine permease

Gene

GNP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity transport of glutamine. Also transport Leu, Ser, Thr, Cys, Met and Asn.3 Publications

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: SGD
  • antiporter activity Source: GO_Central
  • L-proline transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transport Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30029-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.5. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glutamine permease
Gene namesi
Name:GNP1
Ordered Locus Names:YDR508C
ORF Names:D9719.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR508C.
SGDiS000002916. GNP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 154154CytoplasmicSequence analysisAdd
BLAST
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Topological domaini176 – 1772Mitochondrial intermembraneSequence analysis
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21921CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 24021HelicalSequence analysisAdd
BLAST
Topological domaini241 – 26424Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence analysisAdd
BLAST
Topological domaini286 – 2894CytoplasmicSequence analysis
Transmembranei290 – 31021HelicalSequence analysisAdd
BLAST
Topological domaini311 – 34232Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei343 – 36321HelicalSequence analysisAdd
BLAST
Topological domaini364 – 38118CytoplasmicSequence analysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence analysisAdd
BLAST
Topological domaini403 – 43230Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei433 – 45321HelicalSequence analysisAdd
BLAST
Topological domaini454 – 48128CytoplasmicSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 5064Mitochondrial intermembraneSequence analysis
Transmembranei507 – 52721HelicalSequence analysisAdd
BLAST
Topological domaini528 – 56033CytoplasmicSequence analysisAdd
BLAST
Transmembranei561 – 58121HelicalSequence analysisAdd
BLAST
Topological domaini582 – 5909Mitochondrial intermembraneSequence analysis
Transmembranei591 – 61121HelicalSequence analysisAdd
BLAST
Topological domaini612 – 66352CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • mitochondrial membrane Source: UniProtKB-SubCell
  • plasma membrane Source: SGD
  • vacuole Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663High-affinity glutamine permeasePRO_0000054152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphoserineCombined sources
Cross-linki34 – 34Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication
Cross-linki39 – 39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki41 – 41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication
Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei113 – 1131PhosphoserineCombined sources
Modified residuei124 – 1241PhosphoserineCombined sources
Modified residuei127 – 1271PhosphothreonineCombined sources
Cross-linki132 – 132Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP48813.

PTM databases

iPTMnetiP48813.
SwissPalmiP48813.

Interactioni

Protein-protein interaction databases

BioGridi32560. 111 interactions.
DIPiDIP-1616N.
IntActiP48813. 6 interactions.
MINTiMINT-399554.

Structurei

3D structure databases

ProteinModelPortaliP48813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP48813.
KOiK16261.
OMAiFFRITVF.
OrthoDBiEOG092C164R.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLGNRRHGR NNEGSSNMNM NRNDLDDVSH YEMKEIQPKE KQIGSIEPEN
60 70 80 90 100
EVEYFEKTVE KTIENMEYEG EHHASYLRRF IDSFRRAEGS HANSPDSSNS
110 120 130 140 150
NGTTPISTKD SSSQLDNELN RKSSYITVDG IKQSPQEQEQ KQENLKKSIK
160 170 180 190 200
PRHTVMMSLG TGIGTGLLVG NSKVLNNAGP GGLIIGYAIM GSCVYCIIQA
210 220 230 240 250
CGELAVIYSD LIGGFNTYPL FLVDPALGFS VAWLFCLQWL CVCPLELVTA
260 270 280 290 300
SMTIKYWTTS VNPDVFVVIF YVLIVVINVF GAKGYAEADF FFNCCKILMI
310 320 330 340 350
VGFFILAIII DCGGAGTDGY IGSKYWRDPG AFRGDTPIQR FKGVVATFVT
360 370 380 390 400
AAFAFGMSEQ LAMTASEQSN PRKAIPSAAK KMIYRILFVF LASLTLVGFL
410 420 430 440 450
VPYTSDQLLG AAGSATKASP YVIAVSSHGV RVVPHFINAV ILLSVLSVAN
460 470 480 490 500
GAFYTSSRIL MSLAKQGNAP KCFDYIDREG RPAAAMLVSA LFGVIAFCAS
510 520 530 540 550
SKKEEDVFTW LLAISGLSQL FTWITICLSH IRFRRAMKVQ GRSLGEVGYK
560 570 580 590 600
SQVGVWGSAY AVLMMVLALI AQFWVAIAPI GGGGKLSAQS FFENYLAMPI
610 620 630 640 650
WIALYIFYKV WKKDWSLFIP ADKVDLVSHR NIFDEELLKQ EDEEYKERLR
660
NGPYWKRVLD FWC
Length:663
Mass (Da):73,598
Last modified:October 1, 1996 - v2
Checksum:i9A5ED9CF7C1DD977
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti319 – 3191G → S in AAB48002 (PubMed:8660458).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21643 Genomic DNA. Translation: AAB48002.1.
U33057 Genomic DNA. Translation: AAB64950.1.
BK006938 Genomic DNA. Translation: DAA12340.1.
PIRiS69566.
RefSeqiNP_010796.1. NM_001180816.1.

Genome annotation databases

EnsemblFungiiYDR508C; YDR508C; YDR508C.
GeneIDi852121.
KEGGisce:YDR508C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21643 Genomic DNA. Translation: AAB48002.1.
U33057 Genomic DNA. Translation: AAB64950.1.
BK006938 Genomic DNA. Translation: DAA12340.1.
PIRiS69566.
RefSeqiNP_010796.1. NM_001180816.1.

3D structure databases

ProteinModelPortaliP48813.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32560. 111 interactions.
DIPiDIP-1616N.
IntActiP48813. 6 interactions.
MINTiMINT-399554.

Protein family/group databases

TCDBi2.A.3.10.5. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP48813.
SwissPalmiP48813.

Proteomic databases

MaxQBiP48813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR508C; YDR508C; YDR508C.
GeneIDi852121.
KEGGisce:YDR508C.

Organism-specific databases

EuPathDBiFungiDB:YDR508C.
SGDiS000002916. GNP1.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP48813.
KOiK16261.
OMAiFFRITVF.
OrthoDBiEOG092C164R.

Enzyme and pathway databases

BioCyciYEAST:G3O-30029-MONOMER.

Miscellaneous databases

PROiP48813.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNP1_YEAST
AccessioniPrimary (citable) accession number: P48813
Secondary accession number(s): D6VTD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.