Reviewed, UniProtKB/Swiss-Prot P48795 (PYRC_LACLE)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:

pyrC

Organism

Lactobacillus leichmannii

Taxonomic identifier

28039 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Dihydroorotase		PRO_0000147236
Sites
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1; via carbamate group By similarity
	Metal binding	140	1	Zinc 2; via carbamate group By similarity
	Metal binding	177	1	Zinc 2 By similarity
	Metal binding	230	1	Zinc 2 By similarity
	Metal binding	303	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	140	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48795-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 1AB5BC6163C57E30	FASTA	427	46,319
	10 20 30 40 50 60 MAILLKNGLV YQEGEFIKED VLISGSKIQA IGLDLPEEGA EVYDLKGKLL APGLVDIHEH 70 80 90 100 110 120 YREPGFTYKE TIKTGSEAAS RGGFTTVCTM PNVDPIPDDL ETFEKQVALN EANSCVHLKQ 130 140 150 160 170 180 YGAITEDLTS DKVVDMAALK EAGAFAFSND GHGIQQAGTM YEAMQEAAKV GLAICEHIQD 190 200 210 220 230 240 DSLYHHGVMN AGKKAEELGL PGILGVSESA QLARDLVLAQ ATGVHYHACH VSTKESVELI 250 260 270 280 290 300 RIAKEYGINV TAEATPHHLL LSEEEIDGNN GYYKMNPPLR SKEDQFALIE GMLDGTIDLI 310 320 330 340 350 360 ATDHAPHSRE EKAGDMRKAA FGIIGNETAF ACLYTKFVKS GQMDLSLLLD LMSYQPAKLF 370 380 390 400 410 420 GLDAGVLAPG KEADLAVFDL DHAEKLSEED YLSKGVNTPF TGQEVYGMTA LTFVSGKLVY KSKHFAD

References

[1]	"Cloning, sequencing, and characterizing the Lactobacillus leichmannii pyrC gene encoding dihydroorotase." Schenk-Groeninger R., Becker J., Brendel M. Biochimie 77:265-272(1995) [PubMed: 8589056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78999 Genomic DNA. Translation: CAA55633.1.
PIR	T46955.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M38.972.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_LACLE

Accession

Primary (citable) accession number: P48795

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 2, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)