Reviewed,
UniProtKB/Swiss-Prot P48794 (HYALP_MOUSE)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hyaluronidase PH-20 Short name=Hyal-PH20 EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase PH-20 Sperm surface protein PH-20 Sperm adhesion molecule 1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 512 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. |
| Catalytic activity | Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. |
| Subcellular location | |
| Sequence similarities | Belongs to the glycosyl hydrolase 56 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell adhesion |
| Cellular component | Cell membrane Membrane |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond GPI-anchor Glycoprotein Lipoprotein |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesionInferred from electronic annotation. Source: UniProtKB-KW fusion of sperm to egg plasma membraneInferred from electronic annotation. Source: InterPro |
| Cellular component | acrosomal vesicle Inferred from direct assay. Source: MGI anchored to membraneInferred from electronic annotation. Source: UniProtKB-KW external side of plasma membraneInferred from direct assay. Source: MGI |
| Molecular function | hyalurononglucosaminidase activity Ref.2 Inferred from direct assay. Source: MGI protein bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 35 | 35 | By similarity | ||||||||
| Chain | 36 – ? | Hyaluronidase PH-20 | PRO_0000012093 | ||||||||
| Propeptide | ? – 512 | Removed in mature form Potential | PRO_0000012094 | ||||||||
Sites | |||||||||||
| Active site | 147 | 1 | Proton donor By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 46 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 165 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 293 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 368 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 60 ↔ 351 | By similarity | |||||||||
| Disulfide bond | 223 ↔ 237 | By similarity | |||||||||
| Disulfide bond | 376 ↔ 387 | By similarity | |||||||||
| Disulfide bond | 381 ↔ 435 | By similarity | |||||||||
| Disulfide bond | 437 ↔ 464 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 182 | 1 | E → R in AAA76603. Ref.1 | ||||||||
| Sequence conflict | 191 | 1 | F → L in AAA76603. Ref.1 | ||||||||
| Sequence conflict | 271 | 1 | N → H in BAC55070. Ref.2 | ||||||||
| Sequence conflict | 495 | 1 | H → R in BAB24161. Ref.4 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Ramarao C.S., Primakoff P. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Sperm. |
| [2] | "Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg." Baba D., Kashiwabara S., Honda A., Yamagata K., Wu Q., Ikawa M., Okabe M., Baba T. J. Biol. Chem. 277:30310-30314(2002) [PubMed: 12065596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Testis. |
| [3] | "Assessment of contraceptive vaccines based on mouse sperm protein PH-20 (SPAM-1)." Hardy C.M., Mobbs K.J. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BALB/c. Tissue: Testis. |
| [4] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis. |
| [5] | "Biochemical characterization of a glycosylphosphatidylinositol-linked hyaluronidase on mouse sperm." Thaler C.D., Cardullo R.A. Biochemistry 34:7788-7795(1995) [PubMed: 7794889] [Abstract] Cited for: CHARACTERIZATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U33958 mRNA. Translation: AAA76603.1. AB085677 Genomic DNA. Translation: BAC55070.1. AY228460 mRNA. Translation: AAP49832.1. AK005638 mRNA. Translation: BAB24161.1. | |
| IPI | IPI00114402. |
| RefSeq | NP_001073344.1. NP_033267.2. |
| UniGene | Mm.4688 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FCQ based on UniProtKB Q08169. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH56. Glycoside Hydrolase Family 56. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000029682. Mus musculus. [Contig view] |
| GeneID | 20690. |
| KEGG | mmu:20690. |
Organism-specific databases | |
| MGI | MGI:109335. Spam1. |
Phylogenomic databases | |
| HOGENOM | P48794. |
| HOVERGEN | P48794. |
| OMA | P48794. LEYFSEH. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.35. 244. |
Gene expression databases | |
| ArrayExpress | P48794. |
| Bgee | P48794. |
| CleanEx | MM_SPAM1. |
| GermOnline | ENSMUSG00000029682. Mus musculus. |
Family and domain databases | |
| InterPro | IPR013785. Aldolase_TIM. IPR017430. Glyco_hydro_56_Hyaluronidase. IPR001439. Glyco_hydro_56_PH20. IPR001968. Glycoside_hydrolase_family_56. IPR018155. Hyaluronidase. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. |
| PANTHER | PTHR11769. Glyco_hydro_56. 1 hit. PTHR11769:SF4. Glyco_hydro_56_PH20. 1 hit. |
| Pfam | PF01630. Glyco_hydro_56. 1 hit. [Graphical view] |
| PIRSF | PIRSF038193. Hyaluronidase. 1 hit. |
| PRINTS | PR00846. GLHYDRLASE56. PR00848. SPERMPH20. |
| ProDom | PD003549. Glyco_hydro_56. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 299225. |
| SOURCE | Search... |
Entry information
| Entry name | HYALP_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P48794 Secondary accession number(s): Q7TSD6, Q812F4, Q9DAQ1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


