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P48794

- HYALP_MOUSE

UniProt

P48794 - HYALP_MOUSE

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Protein

Hyaluronidase PH-20

Gene

Spam1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: MGI

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: UniProtKB-KW
  3. fusion of sperm to egg plasma membrane Source: InterPro
  4. single fertilization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_196631. Interaction With Cumulus Cells.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20
Gene namesi
Name:Spam1
Synonyms:Ph20, Spam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:109335. Spam1.

Subcellular locationi

GO - Cellular componenti

  1. acrosomal vesicle Source: MGI
  2. anchored component of membrane Source: UniProtKB-KW
  3. external side of plasma membrane Source: MGI
  4. membrane raft Source: MGI
  5. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 512Removed in mature formSequence AnalysisPRO_0000012094
Signal peptidei1 – 3535By similarityAdd
BLAST
Chaini36 – ?Hyaluronidase PH-20PRO_0000012093

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi60 ↔ 351By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 237By similarity
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Disulfide bondi437 ↔ 464By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP48794.

Expressioni

Gene expression databases

BgeeiP48794.
CleanExiMM_SPAM1.
GenevestigatoriP48794.

Interactioni

Protein-protein interaction databases

BioGridi203421. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP48794.
SMRiP48794. Positions 53-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77606.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiP48794.
KOiK01197.
OMAiWDAMSLA.
OrthoDBiEOG74J97S.
PhylomeDBiP48794.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48794-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGELRFKHLF WGSFVESGGT FQTVLIFLLI PCSLTVDYRA APILSNTTFL
60 70 80 90 100
WIWNVPTERC VGNVNDPIDL SFFSLIGSPR KTATGQPVTL FYVDRLGLYP
110 120 130 140 150
HIDANQAEHY GGIPQRGDYQ AHLRKAKTDI EHYIPDDKLG LAIIDWEEWR
160 170 180 190 200
PTWLRNWKPK DNYRNKSIEL VQSTNPGLSI TEATQKAIQQ FEEAGRKFME
210 220 230 240 250
GTLHLGKFLR PNQLWGYYLF PDCYNNKFQD PKYDGQCPAV EKKRNDNLKW
260 270 280 290 300
LWKASTGLYP SVYLKKDLKS NRQATLYVRY RVVEAIRVSK VGNASDPVPI
310 320 330 340 350
FVYIRLVFTD RTSEYLLEDD LVNTIGEIVA LGTSGIIIWD AMSLAQRAAG
360 370 380 390 400
CPILHKYMQT TLNPYIVNVT LAAKMCSQTL CNEKGMCSRR KESSDVYLHL
410 420 430 440 450
NPSHFDIMLT ETGKYEVLGN PRVGDLEYFS EHFKCSCFSR MTCKETSDVK
460 470 480 490 500
NVQDVNVCVG DNVCIKAKVE PNPAFYLLPG KSLLFMTTLG HVLYHLPQDI
510
FVFPRKTLVS TP
Length:512
Mass (Da):58,498
Last modified:May 24, 2004 - v2
Checksum:i6502F9FC75E7C86F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821E → R in AAA76603. 1 PublicationCurated
Sequence conflicti191 – 1911F → L in AAA76603. 1 PublicationCurated
Sequence conflicti271 – 2711N → H in BAC55070. (PubMed:12065596)Curated
Sequence conflicti495 – 4951H → R in BAB24161. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33958 mRNA. Translation: AAA76603.1.
AB085677 Genomic DNA. Translation: BAC55070.1.
AY228460 mRNA. Translation: AAP49832.1.
AK005638 mRNA. Translation: BAB24161.1.
CCDSiCCDS19947.1.
RefSeqiNP_001073344.1. NM_001079875.2.
NP_033267.2. NM_009241.3.
XP_006505088.1. XM_006505025.1.
UniGeneiMm.4688.

Genome annotation databases

EnsembliENSMUST00000031693; ENSMUSP00000031693; ENSMUSG00000029682.
GeneIDi20690.
KEGGimmu:20690.
UCSCiuc009bby.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33958 mRNA. Translation: AAA76603.1 .
AB085677 Genomic DNA. Translation: BAC55070.1 .
AY228460 mRNA. Translation: AAP49832.1 .
AK005638 mRNA. Translation: BAB24161.1 .
CCDSi CCDS19947.1.
RefSeqi NP_001073344.1. NM_001079875.2.
NP_033267.2. NM_009241.3.
XP_006505088.1. XM_006505025.1.
UniGenei Mm.4688.

3D structure databases

ProteinModelPortali P48794.
SMRi P48794. Positions 53-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203421. 1 interaction.

Protein family/group databases

CAZyi GH56. Glycoside Hydrolase Family 56.

Proteomic databases

PRIDEi P48794.

Protocols and materials databases

DNASUi 20690.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031693 ; ENSMUSP00000031693 ; ENSMUSG00000029682 .
GeneIDi 20690.
KEGGi mmu:20690.
UCSCi uc009bby.1. mouse.

Organism-specific databases

CTDi 6677.
MGIi MGI:109335. Spam1.

Phylogenomic databases

eggNOGi NOG77606.
GeneTreei ENSGT00550000074476.
HOGENOMi HOG000015133.
HOVERGENi HBG052053.
InParanoidi P48794.
KOi K01197.
OMAi WDAMSLA.
OrthoDBi EOG74J97S.
PhylomeDBi P48794.
TreeFami TF321598.

Enzyme and pathway databases

Reactomei REACT_196631. Interaction With Cumulus Cells.

Miscellaneous databases

NextBioi 299225.
PROi P48794.
SOURCEi Search...

Gene expression databases

Bgeei P48794.
CleanExi MM_SPAM1.
Genevestigatori P48794.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Ramarao C.S., Primakoff P.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Sperm.
  2. "Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg."
    Baba D., Kashiwabara S., Honda A., Yamagata K., Wu Q., Ikawa M., Okabe M., Baba T.
    J. Biol. Chem. 277:30310-30314(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Testis.
  3. "Assessment of contraceptive vaccines based on mouse sperm protein PH-20 (SPAM-1)."
    Hardy C.M., Mobbs K.J.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/c.
    Tissue: Testis.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "Biochemical characterization of a glycosylphosphatidylinositol-linked hyaluronidase on mouse sperm."
    Thaler C.D., Cardullo R.A.
    Biochemistry 34:7788-7795(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHYALP_MOUSE
AccessioniPrimary (citable) accession number: P48794
Secondary accession number(s): Q7TSD6, Q812F4, Q9DAQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 24, 2004
Last modified: October 1, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3