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Protein

Troponin I, cardiac muscle

Gene

Tnni3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Involved in TNI-TNT interactions1
Sitei98Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi138 – 149By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle contraction Source: MGI
  • cellular calcium ion homeostasis Source: MGI
  • heart contraction Source: MGI
  • heart development Source: MGI
  • negative regulation of ATPase activity Source: MGI
  • regulation of muscle contraction Source: MGI
  • regulation of smooth muscle contraction Source: Ensembl
  • regulation of systemic arterial blood pressure by ischemic conditions Source: MGI
  • skeletal muscle contraction Source: GO_Central
  • striated muscle contraction Source: MGI
  • vasculogenesis Source: MGI
  • ventricular cardiac muscle tissue morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-5578775. Ion homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:Tnni3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98783. Tnni3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • sarcomere Source: MGI
  • troponin complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001861532 – 211Troponin I, cardiac muscleAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei23Phosphoserine; by PKA and PKD/PRKD11 Publication1
Modified residuei24Phosphoserine; by PKA and PKD/PRKD11 Publication1
Modified residuei27PhosphotyrosineBy similarity1
Modified residuei32Phosphothreonine; by STK4/MST1By similarity1
Modified residuei43Phosphoserine; by PKC/PRKCE1 Publication1
Modified residuei45Phosphoserine; by PKC/PRKCE1 Publication1
Modified residuei52Phosphothreonine; by STK4/MST1By similarity1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei130Phosphothreonine; by STK4/MST1By similarity1
Modified residuei144Phosphothreonine; by STK4/MST1By similarity1
Modified residuei151PhosphoserineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei200PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48787.
PRIDEiP48787.

PTM databases

iPTMnetiP48787.
PhosphoSitePlusiP48787.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035458.
CleanExiMM_TNNI3.
ExpressionAtlasiP48787. baseline and differential.
GenevisibleiP48787. MM.

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204266. 3 interactors.
IntActiP48787. 6 interactors.
MINTiMINT-4138144.
STRINGi10090.ENSMUSP00000096458.

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Helixi24 – 31Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JPWNMR-A1-32[»]
ProteinModelPortaliP48787.
SMRiP48787.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48787.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 80Involved in binding TNCAdd BLAST48
Regioni130 – 151Involved in binding TNC and actinAdd BLAST22

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP48787.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
PhylomeDBiP48787.
TreeFamiTF313374.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL
60 70 80 90 100
KTLMLQIAKQ EMEREAEERR GEKGRVLRTR CQPLELDGLG FEELQDLCRQ
110 120 130 140 150
LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI
160 170 180 190 200
SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS
210
GMEGRKKKFE G
Length:211
Mass (Da):24,259
Last modified:January 23, 2007 - v2
Checksum:iFB886B0E87B8D49D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22784 Genomic DNA. Translation: CAA80459.1.
U09181 mRNA. Translation: AAA19657.1.
BC061171 mRNA. Translation: AAH61171.1.
CCDSiCCDS39736.1.
PIRiA53805.
RefSeqiNP_033432.1. NM_009406.3.
UniGeneiMm.27674.

Genome annotation databases

EnsembliENSMUST00000098859; ENSMUSP00000096458; ENSMUSG00000035458.
GeneIDi21954.
KEGGimmu:21954.
UCSCiuc009exv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22784 Genomic DNA. Translation: CAA80459.1.
U09181 mRNA. Translation: AAA19657.1.
BC061171 mRNA. Translation: AAH61171.1.
CCDSiCCDS39736.1.
PIRiA53805.
RefSeqiNP_033432.1. NM_009406.3.
UniGeneiMm.27674.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JPWNMR-A1-32[»]
ProteinModelPortaliP48787.
SMRiP48787.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204266. 3 interactors.
IntActiP48787. 6 interactors.
MINTiMINT-4138144.
STRINGi10090.ENSMUSP00000096458.

PTM databases

iPTMnetiP48787.
PhosphoSitePlusiP48787.

Proteomic databases

PaxDbiP48787.
PRIDEiP48787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098859; ENSMUSP00000096458; ENSMUSG00000035458.
GeneIDi21954.
KEGGimmu:21954.
UCSCiuc009exv.1. mouse.

Organism-specific databases

CTDi7137.
MGIiMGI:98783. Tnni3.

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP48787.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
PhylomeDBiP48787.
TreeFamiTF313374.

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-5578775. Ion homeostasis.

Miscellaneous databases

EvolutionaryTraceiP48787.
PROiP48787.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035458.
CleanExiMM_TNNI3.
ExpressionAtlasiP48787. baseline and differential.
GenevisibleiP48787. MM.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_MOUSE
AccessioniPrimary (citable) accession number: P48787
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.