Reviewed, UniProtKB/Swiss-Prot P48776 (T23O_MOUSE)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	Tdo2
Synonyms:	Tdo

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Domain	Coiled coil
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Tryptophan 2,3-dioxygenase		PRO_0000072400
Regions
	Region	42 – 46	5	Substrate binding By similarity
	Region	72 – 76	5	Substrate binding By similarity
	Coiled coil	229 – 268	40	Potential
Sites
	Metal binding	328	1	Iron (heme axial ligand) By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	151	1	Heme By similarity
	Binding site	342	1	Substrate By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine Ref.3
	Modified residue	155	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	18	1	V → L in AAB60491. Ref.1
	Sequence conflict	229	1	N → K in AAB60491. Ref.1
	Sequence conflict	240	1	R → K in AAB60491. Ref.1
	Sequence conflict	245	1	T → K in AAB60491. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48776-1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 201835A021BE7A24	FASTA	406	47,756
	10 20 30 40 50 60 MSGCPFAGNS VGYTLKNVSM EDNEEDRAQT GVNRASKGGL IYGNYLQLEK ILNAQELQSE 70 80 90 100 110 120 VKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG HVRDERNMLK VIARMHRVVV 130 140 150 160 170 180 IFKLLVQQFS VLETMTALDF NDFREYLSPA SGFQSLQFRL LENKIGVLQS LRVPYNRKHY 190 200 210 220 230 240 RDNFGGDYNE LLLKSEQEQT LLQLVEAWLE RTPGLEPNGF NFWGKFEKNI LKGLEEEFLR 250 260 270 280 290 300 IQAKTDSEEK EEQMAEFRKQ KEVLLCLFDE KRHDYLLSKG ERRLSYRALQ GALMIYFYRE 310 320 330 340 350 360 EPRFQVPFQL LTSLMDIDTL MTKWRYNHVC MVHRMLGTKA GTGGSSGYHY LRSTVSDRYK 370 380 390 400 VFVDLFNLST YLVPRHWVPK MNPIIHKFLY TAEYSDSSYF SSDESD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Novoradovsky A., Goldman D. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2J.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U24493 mRNA. Translation: AAB60491.1. AC159260 Genomic DNA. No translation available.
IPI	IPI00114381.
RefSeq	NP_064295.2.
UniGene	Mm.258622
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48776.
PTM databases
PhosphoSite	P48776.
Proteomic databases
PRIDE	P48776.
Genome annotation databases
Ensembl	ENSMUST00000029645; ENSMUSP00000029645; ENSMUSG00000028011; Mus musculus. [Genome view]
GeneID	56720.
KEGG	mmu:56720.
Organism-specific databases
CTD	56720.
MGI	MGI:1928486. Tdo2.
Phylogenomic databases
HOGENOM	P48776.
HOVERGEN	P48776.
OMA	MKLILHE.
Enzyme and pathway databases
BRENDA	1.13.11.11. 244.
Gene expression databases
ArrayExpress	P48776.
Bgee	P48776.
CleanEx	MM_TDO2.
Genevestigator	P48776.
GermOnline	ENSMUSG00000028011. Mus musculus.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

T23O_MOUSE

Accession

Primary (citable) accession number: P48776

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 20, 2009
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)