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Protein

Tryptophan 2,3-dioxygenase

Gene

Tdo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin (By similarity).By similarity

Catalytic activityi

L-tryptophan + O2 = N-formyl-L-kynurenine.UniRule annotation

Cofactori

hemeUniRule annotationNote: Binds 2 heme groups per tetramer.UniRule annotation

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 1 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (Tdo2), Indoleamine 2,3-dioxygenase 2 (Ido2), Tryptophan 2,3-dioxygenase (Tdo2), Tryptophan 2,3-dioxygenase (Tdo2)
  2. Kynurenine formamidase (Afmid)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441SubstrateUniRule annotation
Binding sitei151 – 1511HemeUniRule annotation
Metal bindingi328 – 3281Iron (heme axial ligand)UniRule annotation
Binding sitei342 – 3421SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Tryptophan catabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.11. 3474.
ReactomeiR-MMU-71240. Tryptophan catabolism.
UniPathwayiUPA00333; UER00453.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 2,3-dioxygenaseUniRule annotation (EC:1.13.11.11UniRule annotation)
Short name:
TDOUniRule annotation
Alternative name(s):
Tryptamin 2,3-dioxygenaseUniRule annotation
Tryptophan oxygenaseUniRule annotation
Short name:
TOUniRule annotation
Short name:
TRPOUniRule annotation
Tryptophan pyrrolaseUniRule annotation
TryptophanaseUniRule annotation
Gene namesi
Name:Tdo2
Synonyms:Tdo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1928486. Tdo2.

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075307.
GuidetoPHARMACOLOGYi2887.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Tryptophan 2,3-dioxygenasePRO_0000072400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48776.
PRIDEiP48776.

PTM databases

iPTMnetiP48776.
PhosphoSiteiP48776.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028011.
CleanExiMM_TDO2.
ExpressionAtlasiP48776. baseline and differential.
GenevisibleiP48776. MM.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029645.

Chemistry

BindingDBiP48776.

Structurei

3D structure databases

ProteinModelPortaliP48776.
SMRiP48776. Positions 41-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 465Substrate bindingUniRule annotation
Regioni72 – 765Substrate bindingUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili231 – 26838UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the tryptophan 2,3-dioxygenase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3906. Eukaryota.
COG3483. LUCA.
HOGENOMiHOG000221584.
HOVERGENiHBG003043.
InParanoidiP48776.
KOiK00453.
OMAiWRYNHVL.
OrthoDBiEOG091G080K.
PhylomeDBiP48776.
TreeFamiTF105827.

Family and domain databases

HAMAPiMF_01972. T23O. 1 hit.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGCPFAGNS VGYTLKNVSM EDNEEDRAQT GVNRASKGGL IYGNYLQLEK
60 70 80 90 100
ILNAQELQSE VKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG
110 120 130 140 150
HVRDERNMLK VIARMHRVVV IFKLLVQQFS VLETMTALDF NDFREYLSPA
160 170 180 190 200
SGFQSLQFRL LENKIGVLQS LRVPYNRKHY RDNFGGDYNE LLLKSEQEQT
210 220 230 240 250
LLQLVEAWLE RTPGLEPNGF NFWGKFEKNI LKGLEEEFLR IQAKTDSEEK
260 270 280 290 300
EEQMAEFRKQ KEVLLCLFDE KRHDYLLSKG ERRLSYRALQ GALMIYFYRE
310 320 330 340 350
EPRFQVPFQL LTSLMDIDTL MTKWRYNHVC MVHRMLGTKA GTGGSSGYHY
360 370 380 390 400
LRSTVSDRYK VFVDLFNLST YLVPRHWVPK MNPIIHKFLY TAEYSDSSYF

SSDESD
Length:406
Mass (Da):47,756
Last modified:January 20, 2009 - v2
Checksum:i201835A021BE7A24
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181V → L in AAB60491 (Ref. 1) Curated
Sequence conflicti229 – 2291N → K in AAB60491 (Ref. 1) Curated
Sequence conflicti240 – 2401R → K in AAB60491 (Ref. 1) Curated
Sequence conflicti245 – 2451T → K in AAB60491 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24493 mRNA. Translation: AAB60491.1.
AC159260 Genomic DNA. No translation available.
CCDSiCCDS17427.1.
RefSeqiNP_064295.2. NM_019911.2.
UniGeneiMm.258622.

Genome annotation databases

EnsembliENSMUST00000029645; ENSMUSP00000029645; ENSMUSG00000028011.
GeneIDi56720.
KEGGimmu:56720.
UCSCiuc008poo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24493 mRNA. Translation: AAB60491.1.
AC159260 Genomic DNA. No translation available.
CCDSiCCDS17427.1.
RefSeqiNP_064295.2. NM_019911.2.
UniGeneiMm.258622.

3D structure databases

ProteinModelPortaliP48776.
SMRiP48776. Positions 41-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029645.

Chemistry

BindingDBiP48776.
ChEMBLiCHEMBL1075307.
GuidetoPHARMACOLOGYi2887.

PTM databases

iPTMnetiP48776.
PhosphoSiteiP48776.

Proteomic databases

PaxDbiP48776.
PRIDEiP48776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029645; ENSMUSP00000029645; ENSMUSG00000028011.
GeneIDi56720.
KEGGimmu:56720.
UCSCiuc008poo.1. mouse.

Organism-specific databases

CTDi6999.
MGIiMGI:1928486. Tdo2.

Phylogenomic databases

eggNOGiKOG3906. Eukaryota.
COG3483. LUCA.
HOGENOMiHOG000221584.
HOVERGENiHBG003043.
InParanoidiP48776.
KOiK00453.
OMAiWRYNHVL.
OrthoDBiEOG091G080K.
PhylomeDBiP48776.
TreeFamiTF105827.

Enzyme and pathway databases

UniPathwayiUPA00333; UER00453.
BRENDAi1.13.11.11. 3474.
ReactomeiR-MMU-71240. Tryptophan catabolism.

Miscellaneous databases

ChiTaRSiTdo2. mouse.
PROiP48776.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028011.
CleanExiMM_TDO2.
ExpressionAtlasiP48776. baseline and differential.
GenevisibleiP48776. MM.

Family and domain databases

HAMAPiMF_01972. T23O. 1 hit.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT23O_MOUSE
AccessioniPrimary (citable) accession number: P48776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 20, 2009
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.