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P48774

- GSTM5_MOUSE

UniProt

P48774 - GSTM5_MOUSE

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Protein

Glutathione S-transferase Mu 5

Gene

Gstm5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191SubstrateBy similarity

GO - Molecular functioni

  1. glutathione binding Source: Ensembl
  2. glutathione transferase activity Source: MGI
  3. protein homodimerization activity Source: MGI

GO - Biological processi

  1. cellular detoxification of nitrogen compound Source: Ensembl
  2. glutathione metabolic process Source: UniProtKB
  3. nitrobenzene metabolic process Source: Ensembl
  4. response to estrogen Source: Ensembl
  5. xenobiotic catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_215316. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 5 (EC:2.5.1.18)
Alternative name(s):
Fibrous sheath component 2
Short name:
Fsc2
GST class-mu 5
Gene namesi
Name:Gstm5
Synonyms:Fsc2, Gstm3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1309466. Gstm5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: Ensembl
  4. sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Glutathione S-transferase Mu 5PRO_0000185829Add
BLAST

Proteomic databases

MaxQBiP48774.
PaxDbiP48774.
PRIDEiP48774.

PTM databases

PhosphoSiteiP48774.

Expressioni

Gene expression databases

BgeeiP48774.
CleanExiMM_GSTM3.
MM_GSTM5.
ExpressionAtlasiP48774. baseline and differential.
GenevestigatoriP48774.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with PFKM isoform 2 and isoform 3 (via N-terminal testis-specific region) (PubMed:19889946).By similarity1 Publication

Protein-protein interaction databases

IntActiP48774. 2 interactions.
MINTiMINT-4096984.
STRINGi10090.ENSMUSP00000004134.

Structurei

3D structure databases

ProteinModelPortaliP48774.
SMRiP48774. Positions 5-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 9188GST N-terminalAdd
BLAST
Domaini93 – 211119GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Glutathione bindingBy similarity
Regioni49 – 535Glutathione bindingBy similarity
Regioni62 – 632Glutathione bindingBy similarity
Regioni75 – 762Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG300089.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP48774.
KOiK00799.
OMAiCYNSDHE.
OrthoDBiEOG7KH9M3.
PhylomeDBiP48774.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48774-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSKSMVLGY WDIRGLAHAI RMLLEFTDTS YEEKRYICGE APDYDRSQWL
60 70 80 90 100
DVKFKLDLDF PNLPYLMDGK NKITQSNAIL RYIARKHNMC GDTEEEKIRV
110 120 130 140 150
DIMENQIMDF RMQLVRLCYN SNHENLKPQY LEQLPAQLKQ FSLFLGKFTW
160 170 180 190 200
FAGEKLTFVD FLTYDVLDQN RIFEPKCLDE FPNLKAFMCR FEALEKIAAF
210 220
LQSDRFFKMP INNKMAKWGN KCLC
Length:224
Mass (Da):26,635
Last modified:February 1, 1996 - v1
Checksum:i70122FD0682237E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24428 mRNA. Translation: AAB04096.1.
AK002750 mRNA. Translation: BAB22327.1.
AK019317 mRNA. Translation: BAB31661.1.
AK028098 mRNA. Translation: BAC25746.1.
BC008206 mRNA. Translation: AAH08206.1.
CCDSiCCDS17741.1.
RefSeqiNP_034490.1. NM_010360.2.
UniGeneiMm.282351.

Genome annotation databases

EnsembliENSMUST00000004134; ENSMUSP00000004134; ENSMUSG00000004032.
GeneIDi14866.
KEGGimmu:14866.
UCSCiuc008qxs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24428 mRNA. Translation: AAB04096.1 .
AK002750 mRNA. Translation: BAB22327.1 .
AK019317 mRNA. Translation: BAB31661.1 .
AK028098 mRNA. Translation: BAC25746.1 .
BC008206 mRNA. Translation: AAH08206.1 .
CCDSi CCDS17741.1.
RefSeqi NP_034490.1. NM_010360.2.
UniGenei Mm.282351.

3D structure databases

ProteinModelPortali P48774.
SMRi P48774. Positions 5-224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48774. 2 interactions.
MINTi MINT-4096984.
STRINGi 10090.ENSMUSP00000004134.

PTM databases

PhosphoSitei P48774.

Proteomic databases

MaxQBi P48774.
PaxDbi P48774.
PRIDEi P48774.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000004134 ; ENSMUSP00000004134 ; ENSMUSG00000004032 .
GeneIDi 14866.
KEGGi mmu:14866.
UCSCi uc008qxs.1. mouse.

Organism-specific databases

CTDi 2949.
MGIi MGI:1309466. Gstm5.

Phylogenomic databases

eggNOGi NOG300089.
GeneTreei ENSGT00550000074559.
HOGENOMi HOG000115735.
HOVERGENi HBG106842.
InParanoidi P48774.
KOi K00799.
OMAi CYNSDHE.
OrthoDBi EOG7KH9M3.
PhylomeDBi P48774.
TreeFami TF353040.

Enzyme and pathway databases

Reactomei REACT_215316. Glutathione conjugation.

Miscellaneous databases

ChiTaRSi GSTM5. mouse.
NextBioi 287121.
PROi P48774.
SOURCEi Search...

Gene expression databases

Bgeei P48774.
CleanExi MM_GSTM3.
MM_GSTM5.
ExpressionAtlasi P48774. baseline and differential.
Genevestigatori P48774.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
PRINTSi PR01267. GSTRNSFRASEM.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a unique mu-class glutathione S-transferase in mouse spermatogenic cells."
    Fulcher K.D., Welch J.E., Klapper D.G., O'Brien D.A., Eddy E.M.
    Mol. Reprod. Dev. 42:415-424(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus, Kidney and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 22-34; 54-70; 71-81; 156-171 AND 197-205, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  5. "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5."
    Nakamura N., Mori C., Eddy E.M.
    Biol. Reprod. 82:504-515(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PFKM.

Entry informationi

Entry nameiGSTM5_MOUSE
AccessioniPrimary (citable) accession number: P48774
Secondary accession number(s): Q545W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3