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Protein

Glutathione S-transferase Mu 5

Gene

Gstm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119SubstrateBy similarity1

GO - Molecular functioni

  • enzyme binding Source: MGI
  • glutathione binding Source: MGI
  • glutathione transferase activity Source: MGI
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 5 (EC:2.5.1.18)
Alternative name(s):
Fibrous sheath component 2
Short name:
Fsc2
GST class-mu 5
Gene namesi
Name:Gstm5
Synonyms:Fsc2, Gstm3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1309466. Gstm5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nucleus Source: MGI
  • sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858291 – 224Glutathione S-transferase Mu 5Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP48774.
MaxQBiP48774.
PaxDbiP48774.
PeptideAtlasiP48774.
PRIDEiP48774.

PTM databases

iPTMnetiP48774.
PhosphoSitePlusiP48774.
SwissPalmiP48774.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004032.
CleanExiMM_GSTM3.
MM_GSTM5.
ExpressionAtlasiP48774. baseline and differential.
GenevisibleiP48774. MM.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with PFKM isoform 2 and isoform 3 (via N-terminal testis-specific region) (PubMed:19889946).By similarity1 Publication

GO - Molecular functioni

  • enzyme binding Source: MGI
  • identical protein binding Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi200098. 1 interactor.
IntActiP48774. 4 interactors.
MINTiMINT-4096984.
STRINGi10090.ENSMUSP00000004134.

Structurei

3D structure databases

ProteinModelPortaliP48774.
SMRiP48774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 91GST N-terminalAdd BLAST88
Domaini93 – 211GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 11Glutathione bindingBy similarity2
Regioni49 – 53Glutathione bindingBy similarity5
Regioni62 – 63Glutathione bindingBy similarity2
Regioni75 – 76Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP48774.
KOiK00799.
OMAiQVMDFRM.
OrthoDBiEOG091G0K2E.
PhylomeDBiP48774.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKSMVLGY WDIRGLAHAI RMLLEFTDTS YEEKRYICGE APDYDRSQWL
60 70 80 90 100
DVKFKLDLDF PNLPYLMDGK NKITQSNAIL RYIARKHNMC GDTEEEKIRV
110 120 130 140 150
DIMENQIMDF RMQLVRLCYN SNHENLKPQY LEQLPAQLKQ FSLFLGKFTW
160 170 180 190 200
FAGEKLTFVD FLTYDVLDQN RIFEPKCLDE FPNLKAFMCR FEALEKIAAF
210 220
LQSDRFFKMP INNKMAKWGN KCLC
Length:224
Mass (Da):26,635
Last modified:February 1, 1996 - v1
Checksum:i70122FD0682237E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24428 mRNA. Translation: AAB04096.1.
AK002750 mRNA. Translation: BAB22327.1.
AK019317 mRNA. Translation: BAB31661.1.
AK028098 mRNA. Translation: BAC25746.1.
BC008206 mRNA. Translation: AAH08206.1.
CCDSiCCDS17741.1.
RefSeqiNP_034490.1. NM_010360.2.
UniGeneiMm.282351.

Genome annotation databases

EnsembliENSMUST00000004134; ENSMUSP00000004134; ENSMUSG00000004032.
GeneIDi14866.
KEGGimmu:14866.
UCSCiuc008qxs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24428 mRNA. Translation: AAB04096.1.
AK002750 mRNA. Translation: BAB22327.1.
AK019317 mRNA. Translation: BAB31661.1.
AK028098 mRNA. Translation: BAC25746.1.
BC008206 mRNA. Translation: AAH08206.1.
CCDSiCCDS17741.1.
RefSeqiNP_034490.1. NM_010360.2.
UniGeneiMm.282351.

3D structure databases

ProteinModelPortaliP48774.
SMRiP48774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200098. 1 interactor.
IntActiP48774. 4 interactors.
MINTiMINT-4096984.
STRINGi10090.ENSMUSP00000004134.

PTM databases

iPTMnetiP48774.
PhosphoSitePlusiP48774.
SwissPalmiP48774.

Proteomic databases

EPDiP48774.
MaxQBiP48774.
PaxDbiP48774.
PeptideAtlasiP48774.
PRIDEiP48774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004134; ENSMUSP00000004134; ENSMUSG00000004032.
GeneIDi14866.
KEGGimmu:14866.
UCSCiuc008qxs.1. mouse.

Organism-specific databases

CTDi2949.
MGIiMGI:1309466. Gstm5.

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiP48774.
KOiK00799.
OMAiQVMDFRM.
OrthoDBiEOG091G0K2E.
PhylomeDBiP48774.
TreeFamiTF353040.

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.

Miscellaneous databases

ChiTaRSiGstm5. mouse.
PROiP48774.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004032.
CleanExiMM_GSTM3.
MM_GSTM5.
ExpressionAtlasiP48774. baseline and differential.
GenevisibleiP48774. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM5_MOUSE
AccessioniPrimary (citable) accession number: P48774
Secondary accession number(s): Q545W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.