Reviewed, UniProtKB/Swiss-Prot P48769 (DCK_RAT)

Names and origin

Protein names

Recommended name:
    Deoxycytidine kinase
      Short name=dCK
    EC=2.7.1.74

Gene names

Name:

Dck

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA) By similarity.
Catalytic activity	NTP + deoxycytidine = NDP + dCMP.
Subunit structure	Homodimer.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the DCK/DGK family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	deoxycytidine metabolic process Inferred from direct assay. Source: RGD drug metabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from direct assay. Source: RGD deoxycytidine kinase activity Inferred from direct assay. Source: RGD drug binding Inferred from direct assay. Source: RGD magnesium ion binding Inferred from direct assay. Source: RGD phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro pyrimidine deoxyribonucleoside binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Deoxycytidine kinase		PRO_0000175092
Regions
	Nucleotide binding	28 – 35	8	ATP Probable
Amino acid modifications
	Modified residue	3	1	Phosphothreonine By similarity
	Modified residue	11	1	Phosphoserine By similarity
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	15	1	Phosphoserine By similarity
	Modified residue	16	1	Phosphoserine By similarity
	Modified residue	35	1	Phosphoserine By similarity
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	72	1	Phosphothreonine By similarity
	Modified residue	74	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48769-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4F6C9FAAC09EDBB7	FASTA	260	30,406
	10 20 30 40 50 60 MATPPKRFCS SPSTSSEGTR IKKISIEGNI AAGKSTFVNI LKQVCEDWEV VPEPVARWCN 70 80 90 100 110 120 VQSTQDEFEE LTTSQKSGGN VLQMMYEKPE RWSFIFQSYA CLSRIRAQLA SLNGSLRDAE 130 140 150 160 170 180 KPVLFFERSV YSDRYIFASN LYESDCMNET EWTIYQDWHD WMNSQFGQSL ELDGIIYLRA 190 200 210 220 230 240 TPEKCLNRIY IRGRDEEQGI PLEYLEKLHY KHESWLLHRT LKTNFEYLQE VPILTLDVNL 250 260 DFKDKEESLV EKVKEFLSTT

References

[1]	"Cloning of the Dck gene encoding rat deoxycytidine kinase." Stegmann A.P., Honders M.W., Willemze R., Landegent J.E. Gene 150:351-354(1994) [PubMed: 7821805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33894 mRNA. Translation: AAA65098.1.
IPI	IPI00193680.
RefSeq	NP_077072.1.
UniGene	Rn.10058
3D structure databases
SMR	P48769. Positions 19-259.
ModBase	Search...
Protein-protein interaction databases
STRING	P48769.
Genome annotation databases
Ensembl	ENSRNOT00000004440; ENSRNOP00000004440; ENSRNOG00000003296; Rattus norvegicus. [Genome view]
GeneID	79127.
KEGG	rno:79127.
UCSC	NM_024158. rat.
Organism-specific databases
CTD	79127.
RGD	620667. Dck.
Phylogenomic databases
eggNOG	roNOG14466.
HOVERGEN	P48769.
InParanoid	P48769.
Enzyme and pathway databases
BRENDA	2.7.1.74. 248.
Gene expression databases
ArrayExpress	P48769.
Genevestigator	P48769.
GermOnline	ENSRNOG00000003296. Rattus norvegicus.
Family and domain databases
InterPro	IPR002624. Deoxynucleoside_kinase. [Graphical view]
PANTHER	PTHR10513. dNK. 1 hit.
Pfam	PF01712. dNK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614566.

Entry information

Entry name

DCK_RAT

Accession

Primary (citable) accession number: P48769

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)