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Protein

Sodium/hydrogen exchanger 2

Gene

Slc9a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.

GO - Molecular functioni

  • sodium:proton antiporter activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425986. Sodium/Proton exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 2
Alternative name(s):
H7
Na(+)/H(+) exchanger 2
Short name:
NHE-2
Solute carrier family 9 member 2
Gene namesi
Name:Slc9a2
Synonyms:Nhe2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi3719. Slc9a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Intramembranei14 – 34Name=A/M1Sequence analysisAdd BLAST21
Topological domaini35 – 80CytoplasmicSequence analysisAdd BLAST46
Intramembranei81 – 101Name=B/M2Sequence analysisAdd BLAST21
Topological domaini102 – 107CytoplasmicSequence analysis6
Transmembranei108 – 128Helical; Name=C/M3Sequence analysisAdd BLAST21
Topological domaini129 – 139ExtracellularSequence analysisAdd BLAST11
Transmembranei140 – 160Helical; Name=D/M4Sequence analysisAdd BLAST21
Topological domaini161 – 169CytoplasmicSequence analysis9
Transmembranei170 – 190Helical; Name=E/M5Sequence analysisAdd BLAST21
Topological domaini191 – 209ExtracellularSequence analysisAdd BLAST19
Transmembranei210 – 230Helical; Name=F/M5ASequence analysisAdd BLAST21
Topological domaini231 – 237CytoplasmicSequence analysis7
Transmembranei238 – 258Helical; Name=G/M5BSequence analysisAdd BLAST21
Topological domaini259 – 278ExtracellularSequence analysisAdd BLAST20
Transmembranei279 – 299Helical; Name=H/M6Sequence analysisAdd BLAST21
Topological domaini300 – 308CytoplasmicSequence analysis9
Transmembranei309 – 329Helical; Name=I/M7Sequence analysisAdd BLAST21
Topological domaini330 – 361ExtracellularSequence analysisAdd BLAST32
Transmembranei362 – 382Helical; Name=J/M8Sequence analysisAdd BLAST21
Topological domaini383 – 392CytoplasmicSequence analysis10
Transmembranei393 – 413Helical; Name=K/M9Sequence analysisAdd BLAST21
Topological domaini414 – 430ExtracellularSequence analysisAdd BLAST17
Intramembranei431 – 451Name=LSequence analysisAdd BLAST21
Topological domaini452 – 459ExtracellularSequence analysis8
Transmembranei460 – 480Helical; Name=M13Sequence analysisAdd BLAST21
Topological domaini481 – 813CytoplasmicSequence analysisAdd BLAST333

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000523541 – 813Sodium/hydrogen exchanger 2Add BLAST813

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi351N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylated (Possible).

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48763.

PTM databases

iPTMnetiP48763.
PhosphoSitePlusiP48763.

Expressioni

Tissue specificityi

Predominantly in small intestine, colon, and stomach, with much lower levels in skeletal muscle, kidney, brain, testis, uterus, heart and lung.

Gene expression databases

BgeeiENSRNOG00000015567.
GenevisibleiP48763. RN.

Interactioni

Subunit structurei

Interacts with CHP1 and CHP2.1 Publication

Protein-protein interaction databases

IntActiP48763. 2 interactors.
STRINGi10116.ENSRNOP00000021270.

Structurei

3D structure databases

ProteinModelPortaliP48763.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiP48763.
KOiK14722.
OMAiAYKMEWK.
OrthoDBiEOG091G02Q0.
PhylomeDBiP48763.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
IPR032103. NHE_CaM-bd.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF16644. NEXCaM_BD. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P48763-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPSGTAHRM RAPLSWLLLL LLSLQVAVPA GALAETLLDA PGARGASSNP
60 70 80 90 100
PSPASVVAPG TTPFEESRLP VFTLDYPHVQ IPFEITLWIL LASLAKIGFH
110 120 130 140 150
LYHKLPTIVP ESCLLIMVGL LLGGIIFGVD EKSPPAMKTD VFFLYLLPPI
160 170 180 190 200
VLDAGYFMPT RPFFENLGTI FWYAVVGTLW NSIGIGLSLF GICQIEAFGL
210 220 230 240 250
SDITLLQNLL FGSLISAVDP VAVLAVFENI HVNEQLYILV FGESLLNDAV
260 270 280 290 300
TVVLYNLFKS FCQMKTIQTV DVFAGIANFF VVGIGGVLIG ILLGFIAAFT
310 320 330 340 350
TRFTHNIRVI EPLFVFLYSY LSYITAEMFH LSGIMAITAC AMTMNKYVEE
360 370 380 390 400
NVSQKSYTTI KYFMKMLSSV SETLIFIFMG VSTVGKNHEW NWAFVCFTLA
410 420 430 440 450
FCLIWRALGV FVLTQVINWF RTIPLTFKDQ FIIAYGGLRG AICFALVFLL
460 470 480 490 500
PATVFPRKKL FITAAIVVIF FTVFILGITI RPLVEFLDVK RSNKKQQAVS
510 520 530 540 550
EEIHCRFFDH VKTGIEDVCG HWGHNFWRDK FKKFDDKYLR KLLIRENQPK
560 570 580 590 600
SSIVSLYKKL EIKHAIEMAE TGMISTVPSF ASLNDCREEK IRKLTPGEMD
610 620 630 640 650
EIREILSRNL YQIRQRTLSY NRHNLTADTS ERQAKEILIR RRHSLRESLR
660 670 680 690 700
KDNSLNRERR ASTSTSRYLS LPKNTKLPEK LQKKNKVSNA DGNSSDSDMD
710 720 730 740 750
GTTVLNLQPR ARRFLPDQFS KKASPAYKME WKNEVDVGSA RAPPSVTPAP
760 770 780 790 800
RSKEGGTQTP GVLRQPLLSK DQRFGRGRED SLTEDVPPKP PPRLVRRASE
810
PGNRKGRLGN EKP
Length:813
Mass (Da):91,403
Last modified:February 1, 1996 - v1
Checksum:i29727267D7085845
GO
Isoform Short (identifier: P48763-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Show »
Length:697
Mass (Da):79,063
Checksum:iDD5C1A503CAE9ACE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti504H → HW in AAB36180 (PubMed:8595899).Curated1
Sequence conflicti610 – 616LYQIRQR → SLSNPPA in AAB36180 (PubMed:8595899).Curated7
Sequence conflicti742A → P in AAB36180 (PubMed:8595899).Curated1
Sequence conflicti786V → G in AAA75406 (PubMed:7683411).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0033941 – 116Missing in isoform Short. 2 PublicationsAdd BLAST116

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11236 mRNA. Translation: AAA72350.1.
L11004 mRNA. Translation: AAA75406.1.
S81591 mRNA. Translation: AAB36180.1.
PIRiA46748.
A57644.
RefSeqiNP_001106806.1. NM_001113335.1. [P48763-1]
NP_036785.2. NM_012653.2. [P48763-2]
UniGeneiRn.11047.

Genome annotation databases

EnsembliENSRNOT00000021270; ENSRNOP00000021270; ENSRNOG00000015567. [P48763-1]
GeneIDi24783.
KEGGirno:24783.
UCSCiRGD:3719. rat. [P48763-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11236 mRNA. Translation: AAA72350.1.
L11004 mRNA. Translation: AAA75406.1.
S81591 mRNA. Translation: AAB36180.1.
PIRiA46748.
A57644.
RefSeqiNP_001106806.1. NM_001113335.1. [P48763-1]
NP_036785.2. NM_012653.2. [P48763-2]
UniGeneiRn.11047.

3D structure databases

ProteinModelPortaliP48763.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP48763. 2 interactors.
STRINGi10116.ENSRNOP00000021270.

PTM databases

iPTMnetiP48763.
PhosphoSitePlusiP48763.

Proteomic databases

PaxDbiP48763.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021270; ENSRNOP00000021270; ENSRNOG00000015567. [P48763-1]
GeneIDi24783.
KEGGirno:24783.
UCSCiRGD:3719. rat. [P48763-1]

Organism-specific databases

CTDi6549.
RGDi3719. Slc9a2.

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiP48763.
KOiK14722.
OMAiAYKMEWK.
OrthoDBiEOG091G02Q0.
PhylomeDBiP48763.
TreeFamiTF317212.

Enzyme and pathway databases

ReactomeiR-RNO-425986. Sodium/Proton exchangers.

Miscellaneous databases

PROiP48763.

Gene expression databases

BgeeiENSRNOG00000015567.
GenevisibleiP48763. RN.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
IPR032103. NHE_CaM-bd.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF16644. NEXCaM_BD. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSL9A2_RAT
AccessioniPrimary (citable) accession number: P48763
Secondary accession number(s): Q16434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.Curated
PubMed:8595899 sequence was originally thought to originate from human.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.