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P48763

- SL9A2_RAT

UniProt

P48763 - SL9A2_RAT

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Protein

Sodium/hydrogen exchanger 2

Gene

Slc9a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.

GO - Molecular functioni

  1. sodium:proton antiporter activity Source: RGD

GO - Biological processi

  1. protein localization Source: Ensembl
  2. regulation of pH Source: Ensembl
  3. sodium ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 2
Alternative name(s):
H7
Na(+)/H(+) exchanger 2
Short name:
NHE-2
Solute carrier family 9 member 2
Gene namesi
Name:Slc9a2
Synonyms:Nhe2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi3719. Slc9a2.

Subcellular locationi

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Sodium/hydrogen exchanger 2PRO_0000052354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylated (Possible).

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48763.
PRIDEiP48763.

Expressioni

Tissue specificityi

Predominantly in small intestine, colon, and stomach, with much lower levels in skeletal muscle, kidney, brain, testis, uterus, heart and lung.

Gene expression databases

GenevestigatoriP48763.

Interactioni

Subunit structurei

Interacts with CHP1 and CHP2.1 Publication

Protein-protein interaction databases

IntActiP48763. 2 interactions.
STRINGi10116.ENSRNOP00000021270.

Structurei

3D structure databases

ProteinModelPortaliP48763.
SMRiP48763. Positions 137-162, 231-256, 319-346.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini35 – 8046CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini102 – 1076CytoplasmicSequence Analysis
Topological domaini129 – 13911ExtracellularSequence AnalysisAdd
BLAST
Topological domaini161 – 1699CytoplasmicSequence Analysis
Topological domaini191 – 20919ExtracellularSequence AnalysisAdd
BLAST
Topological domaini231 – 2377CytoplasmicSequence Analysis
Topological domaini259 – 27820ExtracellularSequence AnalysisAdd
BLAST
Topological domaini300 – 3089CytoplasmicSequence Analysis
Topological domaini330 – 36132ExtracellularSequence AnalysisAdd
BLAST
Topological domaini383 – 39210CytoplasmicSequence Analysis
Topological domaini414 – 43017ExtracellularSequence AnalysisAdd
BLAST
Topological domaini452 – 4598ExtracellularSequence Analysis
Topological domaini481 – 813333CytoplasmicSequence AnalysisAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei14 – 3421Name=A/M1Sequence AnalysisAdd
BLAST
Intramembranei81 – 10121Name=B/M2Sequence AnalysisAdd
BLAST
Intramembranei431 – 45121Name=LSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei108 – 12821Helical; Name=C/M3Sequence AnalysisAdd
BLAST
Transmembranei140 – 16021Helical; Name=D/M4Sequence AnalysisAdd
BLAST
Transmembranei170 – 19021Helical; Name=E/M5Sequence AnalysisAdd
BLAST
Transmembranei210 – 23021Helical; Name=F/M5ASequence AnalysisAdd
BLAST
Transmembranei238 – 25821Helical; Name=G/M5BSequence AnalysisAdd
BLAST
Transmembranei279 – 29921Helical; Name=H/M6Sequence AnalysisAdd
BLAST
Transmembranei309 – 32921Helical; Name=I/M7Sequence AnalysisAdd
BLAST
Transmembranei362 – 38221Helical; Name=J/M8Sequence AnalysisAdd
BLAST
Transmembranei393 – 41321Helical; Name=K/M9Sequence AnalysisAdd
BLAST
Transmembranei460 – 48021Helical; Name=M13Sequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiP48763.
KOiK14722.
OMAiPQSYKME.
OrthoDBiEOG7PCJGB.
PhylomeDBiP48763.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P48763-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPSGTAHRM RAPLSWLLLL LLSLQVAVPA GALAETLLDA PGARGASSNP
60 70 80 90 100
PSPASVVAPG TTPFEESRLP VFTLDYPHVQ IPFEITLWIL LASLAKIGFH
110 120 130 140 150
LYHKLPTIVP ESCLLIMVGL LLGGIIFGVD EKSPPAMKTD VFFLYLLPPI
160 170 180 190 200
VLDAGYFMPT RPFFENLGTI FWYAVVGTLW NSIGIGLSLF GICQIEAFGL
210 220 230 240 250
SDITLLQNLL FGSLISAVDP VAVLAVFENI HVNEQLYILV FGESLLNDAV
260 270 280 290 300
TVVLYNLFKS FCQMKTIQTV DVFAGIANFF VVGIGGVLIG ILLGFIAAFT
310 320 330 340 350
TRFTHNIRVI EPLFVFLYSY LSYITAEMFH LSGIMAITAC AMTMNKYVEE
360 370 380 390 400
NVSQKSYTTI KYFMKMLSSV SETLIFIFMG VSTVGKNHEW NWAFVCFTLA
410 420 430 440 450
FCLIWRALGV FVLTQVINWF RTIPLTFKDQ FIIAYGGLRG AICFALVFLL
460 470 480 490 500
PATVFPRKKL FITAAIVVIF FTVFILGITI RPLVEFLDVK RSNKKQQAVS
510 520 530 540 550
EEIHCRFFDH VKTGIEDVCG HWGHNFWRDK FKKFDDKYLR KLLIRENQPK
560 570 580 590 600
SSIVSLYKKL EIKHAIEMAE TGMISTVPSF ASLNDCREEK IRKLTPGEMD
610 620 630 640 650
EIREILSRNL YQIRQRTLSY NRHNLTADTS ERQAKEILIR RRHSLRESLR
660 670 680 690 700
KDNSLNRERR ASTSTSRYLS LPKNTKLPEK LQKKNKVSNA DGNSSDSDMD
710 720 730 740 750
GTTVLNLQPR ARRFLPDQFS KKASPAYKME WKNEVDVGSA RAPPSVTPAP
760 770 780 790 800
RSKEGGTQTP GVLRQPLLSK DQRFGRGRED SLTEDVPPKP PPRLVRRASE
810
PGNRKGRLGN EKP
Length:813
Mass (Da):91,403
Last modified:February 1, 1996 - v1
Checksum:i29727267D7085845
GO
Isoform Short (identifier: P48763-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Show »
Length:697
Mass (Da):79,063
Checksum:iDD5C1A503CAE9ACE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti504 – 5041H → HW in AAB36180. (PubMed:8595899)Curated
Sequence conflicti610 – 6167LYQIRQR → SLSNPPA in AAB36180. (PubMed:8595899)Curated
Sequence conflicti742 – 7421A → P in AAB36180. (PubMed:8595899)Curated
Sequence conflicti786 – 7861V → G in AAA75406. (PubMed:7683411)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 116116Missing in isoform Short. 2 PublicationsVSP_003394Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11236 mRNA. Translation: AAA72350.1.
L11004 mRNA. Translation: AAA75406.1.
S81591 mRNA. Translation: AAB36180.1.
PIRiA46748.
A57644.
RefSeqiNP_001106806.1. NM_001113335.1. [P48763-1]
NP_036785.2. NM_012653.2. [P48763-2]
UniGeneiRn.11047.

Genome annotation databases

EnsembliENSRNOT00000021270; ENSRNOP00000021270; ENSRNOG00000015567. [P48763-1]
ENSRNOT00000066000; ENSRNOP00000059336; ENSRNOG00000015567. [P48763-2]
GeneIDi24783.
KEGGirno:24783.
UCSCiRGD:3719. rat. [P48763-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11236 mRNA. Translation: AAA72350.1 .
L11004 mRNA. Translation: AAA75406.1 .
S81591 mRNA. Translation: AAB36180.1 .
PIRi A46748.
A57644.
RefSeqi NP_001106806.1. NM_001113335.1. [P48763-1 ]
NP_036785.2. NM_012653.2. [P48763-2 ]
UniGenei Rn.11047.

3D structure databases

ProteinModelPortali P48763.
SMRi P48763. Positions 137-162, 231-256, 319-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P48763. 2 interactions.
STRINGi 10116.ENSRNOP00000021270.

Proteomic databases

PaxDbi P48763.
PRIDEi P48763.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021270 ; ENSRNOP00000021270 ; ENSRNOG00000015567 . [P48763-1 ]
ENSRNOT00000066000 ; ENSRNOP00000059336 ; ENSRNOG00000015567 . [P48763-2 ]
GeneIDi 24783.
KEGGi rno:24783.
UCSCi RGD:3719. rat. [P48763-1 ]

Organism-specific databases

CTDi 6549.
RGDi 3719. Slc9a2.

Phylogenomic databases

eggNOGi COG0025.
GeneTreei ENSGT00760000119074.
HOGENOMi HOG000247044.
HOVERGENi HBG052615.
InParanoidi P48763.
KOi K14722.
OMAi PQSYKME.
OrthoDBi EOG7PCJGB.
PhylomeDBi P48763.
TreeFami TF317212.

Miscellaneous databases

NextBioi 604396.
PROi P48763.

Gene expression databases

Genevestigatori P48763.

Family and domain databases

InterProi IPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
[Graphical view ]
PANTHERi PTHR10110. PTHR10110. 1 hit.
Pfami PF00999. Na_H_Exchanger. 1 hit.
[Graphical view ]
PRINTSi PR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Primary structure and functional expression of a novel gastrointestinal isoform of the rat Na/H exchanger."
    Wang Z., Orlowski J., Shull G.E.
    J. Biol. Chem. 268:11925-11928(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Stomach.
  2. "Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2)."
    Collins J.F., Honda T., Knobel S., Bulus N.M., Conary J., Dubois R., Ghishan F.K.
    Proc. Natl. Acad. Sci. U.S.A. 90:3938-3942(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Strain: Sprague-Dawley.
    Tissue: Small intestine.
  3. "Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+ exchanger (SLC9A2)."
    Ghishan F.K., Knobel S.M., Summar M.
    Genomics 30:25-30(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Tissue: Liver.
  4. "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium."
    Inoue H., Nakamura Y., Nagita M., Takai T., Masuda M., Nakamura N., Kanazawa H.
    Biol. Pharm. Bull. 26:148-155(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHP1 AND CHP2.

Entry informationi

Entry nameiSL9A2_RAT
AccessioniPrimary (citable) accession number: P48763
Secondary accession number(s): Q16434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.Curated
PubMed:8595899 sequence was originally thought to originate from human.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3