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P48763

- SL9A2_RAT

UniProt

P48763 - SL9A2_RAT

Protein

Sodium/hydrogen exchanger 2

Gene

Slc9a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.

    GO - Molecular functioni

    1. sodium:proton antiporter activity Source: RGD

    GO - Biological processi

    1. protein localization Source: Ensembl
    2. regulation of pH Source: Ensembl
    3. sodium ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Antiport, Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/hydrogen exchanger 2
    Alternative name(s):
    H7
    Na(+)/H(+) exchanger 2
    Short name:
    NHE-2
    Solute carrier family 9 member 2
    Gene namesi
    Name:Slc9a2
    Synonyms:Nhe2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi3719. Slc9a2.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 813813Sodium/hydrogen exchanger 2PRO_0000052354Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Phosphorylated (Possible).

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP48763.
    PRIDEiP48763.

    Expressioni

    Tissue specificityi

    Predominantly in small intestine, colon, and stomach, with much lower levels in skeletal muscle, kidney, brain, testis, uterus, heart and lung.

    Gene expression databases

    GenevestigatoriP48763.

    Interactioni

    Subunit structurei

    Interacts with CHP1 and CHP2.1 Publication

    Protein-protein interaction databases

    IntActiP48763. 2 interactions.
    STRINGi10116.ENSRNOP00000021270.

    Structurei

    3D structure databases

    ProteinModelPortaliP48763.
    SMRiP48763. Positions 137-162, 231-256, 319-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1313CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini35 – 8046CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini102 – 1076CytoplasmicSequence Analysis
    Topological domaini129 – 13911ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini161 – 1699CytoplasmicSequence Analysis
    Topological domaini191 – 20919ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini231 – 2377CytoplasmicSequence Analysis
    Topological domaini259 – 27820ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini300 – 3089CytoplasmicSequence Analysis
    Topological domaini330 – 36132ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini383 – 39210CytoplasmicSequence Analysis
    Topological domaini414 – 43017ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini452 – 4598ExtracellularSequence Analysis
    Topological domaini481 – 813333CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei14 – 3421Name=A/M1Sequence AnalysisAdd
    BLAST
    Intramembranei81 – 10121Name=B/M2Sequence AnalysisAdd
    BLAST
    Intramembranei431 – 45121Name=LSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei108 – 12821Helical; Name=C/M3Sequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021Helical; Name=D/M4Sequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021Helical; Name=E/M5Sequence AnalysisAdd
    BLAST
    Transmembranei210 – 23021Helical; Name=F/M5ASequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821Helical; Name=G/M5BSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29921Helical; Name=H/M6Sequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921Helical; Name=I/M7Sequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221Helical; Name=J/M8Sequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321Helical; Name=K/M9Sequence AnalysisAdd
    BLAST
    Transmembranei460 – 48021Helical; Name=M13Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0025.
    GeneTreeiENSGT00560000077158.
    HOGENOMiHOG000247044.
    HOVERGENiHBG052615.
    InParanoidiP48763.
    KOiK14722.
    OMAiPQSYKME.
    OrthoDBiEOG7PCJGB.
    PhylomeDBiP48763.
    TreeFamiTF317212.

    Family and domain databases

    InterProiIPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR001953. Na/H_exchanger_2.
    IPR004709. NaH_exchanger.
    [Graphical view]
    PANTHERiPTHR10110. PTHR10110. 1 hit.
    PfamiPF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    PRINTSiPR01084. NAHEXCHNGR.
    PR01086. NAHEXCHNGR2.
    TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P48763-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGPSGTAHRM RAPLSWLLLL LLSLQVAVPA GALAETLLDA PGARGASSNP    50
    PSPASVVAPG TTPFEESRLP VFTLDYPHVQ IPFEITLWIL LASLAKIGFH 100
    LYHKLPTIVP ESCLLIMVGL LLGGIIFGVD EKSPPAMKTD VFFLYLLPPI 150
    VLDAGYFMPT RPFFENLGTI FWYAVVGTLW NSIGIGLSLF GICQIEAFGL 200
    SDITLLQNLL FGSLISAVDP VAVLAVFENI HVNEQLYILV FGESLLNDAV 250
    TVVLYNLFKS FCQMKTIQTV DVFAGIANFF VVGIGGVLIG ILLGFIAAFT 300
    TRFTHNIRVI EPLFVFLYSY LSYITAEMFH LSGIMAITAC AMTMNKYVEE 350
    NVSQKSYTTI KYFMKMLSSV SETLIFIFMG VSTVGKNHEW NWAFVCFTLA 400
    FCLIWRALGV FVLTQVINWF RTIPLTFKDQ FIIAYGGLRG AICFALVFLL 450
    PATVFPRKKL FITAAIVVIF FTVFILGITI RPLVEFLDVK RSNKKQQAVS 500
    EEIHCRFFDH VKTGIEDVCG HWGHNFWRDK FKKFDDKYLR KLLIRENQPK 550
    SSIVSLYKKL EIKHAIEMAE TGMISTVPSF ASLNDCREEK IRKLTPGEMD 600
    EIREILSRNL YQIRQRTLSY NRHNLTADTS ERQAKEILIR RRHSLRESLR 650
    KDNSLNRERR ASTSTSRYLS LPKNTKLPEK LQKKNKVSNA DGNSSDSDMD 700
    GTTVLNLQPR ARRFLPDQFS KKASPAYKME WKNEVDVGSA RAPPSVTPAP 750
    RSKEGGTQTP GVLRQPLLSK DQRFGRGRED SLTEDVPPKP PPRLVRRASE 800
    PGNRKGRLGN EKP 813
    Length:813
    Mass (Da):91,403
    Last modified:February 1, 1996 - v1
    Checksum:i29727267D7085845
    GO
    Isoform Short (identifier: P48763-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-116: Missing.

    Show »
    Length:697
    Mass (Da):79,063
    Checksum:iDD5C1A503CAE9ACE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti504 – 5041H → HW in AAB36180. (PubMed:8595899)Curated
    Sequence conflicti610 – 6167LYQIRQR → SLSNPPA in AAB36180. (PubMed:8595899)Curated
    Sequence conflicti742 – 7421A → P in AAB36180. (PubMed:8595899)Curated
    Sequence conflicti786 – 7861V → G in AAA75406. (PubMed:7683411)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 116116Missing in isoform Short. 2 PublicationsVSP_003394Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11236 mRNA. Translation: AAA72350.1.
    L11004 mRNA. Translation: AAA75406.1.
    S81591 mRNA. Translation: AAB36180.1.
    PIRiA46748.
    A57644.
    RefSeqiNP_001106806.1. NM_001113335.1. [P48763-1]
    NP_036785.2. NM_012653.2. [P48763-2]
    UniGeneiRn.11047.

    Genome annotation databases

    EnsembliENSRNOT00000021270; ENSRNOP00000021270; ENSRNOG00000015567. [P48763-1]
    ENSRNOT00000066000; ENSRNOP00000059336; ENSRNOG00000015567. [P48763-2]
    GeneIDi24783.
    KEGGirno:24783.
    UCSCiRGD:3719. rat. [P48763-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11236 mRNA. Translation: AAA72350.1 .
    L11004 mRNA. Translation: AAA75406.1 .
    S81591 mRNA. Translation: AAB36180.1 .
    PIRi A46748.
    A57644.
    RefSeqi NP_001106806.1. NM_001113335.1. [P48763-1 ]
    NP_036785.2. NM_012653.2. [P48763-2 ]
    UniGenei Rn.11047.

    3D structure databases

    ProteinModelPortali P48763.
    SMRi P48763. Positions 137-162, 231-256, 319-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P48763. 2 interactions.
    STRINGi 10116.ENSRNOP00000021270.

    Proteomic databases

    PaxDbi P48763.
    PRIDEi P48763.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021270 ; ENSRNOP00000021270 ; ENSRNOG00000015567 . [P48763-1 ]
    ENSRNOT00000066000 ; ENSRNOP00000059336 ; ENSRNOG00000015567 . [P48763-2 ]
    GeneIDi 24783.
    KEGGi rno:24783.
    UCSCi RGD:3719. rat. [P48763-1 ]

    Organism-specific databases

    CTDi 6549.
    RGDi 3719. Slc9a2.

    Phylogenomic databases

    eggNOGi COG0025.
    GeneTreei ENSGT00560000077158.
    HOGENOMi HOG000247044.
    HOVERGENi HBG052615.
    InParanoidi P48763.
    KOi K14722.
    OMAi PQSYKME.
    OrthoDBi EOG7PCJGB.
    PhylomeDBi P48763.
    TreeFami TF317212.

    Miscellaneous databases

    NextBioi 604396.
    PROi P48763.

    Gene expression databases

    Genevestigatori P48763.

    Family and domain databases

    InterProi IPR006153. Cation/H_exchanger.
    IPR018422. Cation/H_exchanger_CPA1.
    IPR001953. Na/H_exchanger_2.
    IPR004709. NaH_exchanger.
    [Graphical view ]
    PANTHERi PTHR10110. PTHR10110. 1 hit.
    Pfami PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    PRINTSi PR01084. NAHEXCHNGR.
    PR01086. NAHEXCHNGR2.
    TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of a novel gastrointestinal isoform of the rat Na/H exchanger."
      Wang Z., Orlowski J., Shull G.E.
      J. Biol. Chem. 268:11925-11928(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
      Tissue: Stomach.
    2. "Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2)."
      Collins J.F., Honda T., Knobel S., Bulus N.M., Conary J., Dubois R., Ghishan F.K.
      Proc. Natl. Acad. Sci. U.S.A. 90:3938-3942(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: Sprague-Dawley.
      Tissue: Small intestine.
    3. "Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+ exchanger (SLC9A2)."
      Ghishan F.K., Knobel S.M., Summar M.
      Genomics 30:25-30(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Tissue: Liver.
    4. "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium."
      Inoue H., Nakamura Y., Nagita M., Takai T., Masuda M., Nakamura N., Kanazawa H.
      Biol. Pharm. Bull. 26:148-155(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHP1 AND CHP2.

    Entry informationi

    Entry nameiSL9A2_RAT
    AccessioniPrimary (citable) accession number: P48763
    Secondary accession number(s): Q16434
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.Curated
    PubMed:8595899 sequence was originally thought to originate from human.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3