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P48763 (SL9A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 2
Alternative name(s):
H7
Na(+)/H(+) exchanger 2
Short name=NHE-2
Solute carrier family 9 member 2
Gene names
Name:Slc9a2
Synonyms:Nhe2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.

Subunit structure

Interacts with CHP1 and CHP2. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Predominantly in small intestine, colon, and stomach, with much lower levels in skeletal muscle, kidney, brain, testis, uterus, heart and lung.

Post-translational modification

Phosphorylated (Possible).

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.

Ref.3 sequence was originally thought to originate from human.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P48763-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P48763-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Sodium/hydrogen exchanger 2
PRO_0000052354

Regions

Topological domain1 – 1313Cytoplasmic Potential
Intramembrane14 – 3421Name=A/M1; Potential
Topological domain35 – 8046Cytoplasmic Potential
Intramembrane81 – 10121Name=B/M2; Potential
Topological domain102 – 1076Cytoplasmic Potential
Transmembrane108 – 12821Helical; Name=C/M3; Potential
Topological domain129 – 13911Extracellular Potential
Transmembrane140 – 16021Helical; Name=D/M4; Potential
Topological domain161 – 1699Cytoplasmic Potential
Transmembrane170 – 19021Helical; Name=E/M5; Potential
Topological domain191 – 20919Extracellular Potential
Transmembrane210 – 23021Helical; Name=F/M5A; Potential
Topological domain231 – 2377Cytoplasmic Potential
Transmembrane238 – 25821Helical; Name=G/M5B; Potential
Topological domain259 – 27820Extracellular Potential
Transmembrane279 – 29921Helical; Name=H/M6; Potential
Topological domain300 – 3089Cytoplasmic Potential
Transmembrane309 – 32921Helical; Name=I/M7; Potential
Topological domain330 – 36132Extracellular Potential
Transmembrane362 – 38221Helical; Name=J/M8; Potential
Topological domain383 – 39210Cytoplasmic Potential
Transmembrane393 – 41321Helical; Name=K/M9; Potential
Topological domain414 – 43017Extracellular Potential
Intramembrane431 – 45121Name=L; Potential
Topological domain452 – 4598Extracellular Potential
Transmembrane460 – 48021Helical; Name=M13; Potential
Topological domain481 – 813333Cytoplasmic Potential

Amino acid modifications

Glycosylation3511N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 116116Missing in isoform Short.
VSP_003394

Experimental info

Sequence conflict5041H → HW in AAB36180. Ref.3
Sequence conflict610 – 6167LYQIRQR → SLSNPPA in AAB36180. Ref.3
Sequence conflict7421A → P in AAB36180. Ref.3
Sequence conflict7861V → G in AAA75406. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 29727267D7085845

FASTA81391,403
        10         20         30         40         50         60 
MGPSGTAHRM RAPLSWLLLL LLSLQVAVPA GALAETLLDA PGARGASSNP PSPASVVAPG 

        70         80         90        100        110        120 
TTPFEESRLP VFTLDYPHVQ IPFEITLWIL LASLAKIGFH LYHKLPTIVP ESCLLIMVGL 

       130        140        150        160        170        180 
LLGGIIFGVD EKSPPAMKTD VFFLYLLPPI VLDAGYFMPT RPFFENLGTI FWYAVVGTLW 

       190        200        210        220        230        240 
NSIGIGLSLF GICQIEAFGL SDITLLQNLL FGSLISAVDP VAVLAVFENI HVNEQLYILV 

       250        260        270        280        290        300 
FGESLLNDAV TVVLYNLFKS FCQMKTIQTV DVFAGIANFF VVGIGGVLIG ILLGFIAAFT 

       310        320        330        340        350        360 
TRFTHNIRVI EPLFVFLYSY LSYITAEMFH LSGIMAITAC AMTMNKYVEE NVSQKSYTTI 

       370        380        390        400        410        420 
KYFMKMLSSV SETLIFIFMG VSTVGKNHEW NWAFVCFTLA FCLIWRALGV FVLTQVINWF 

       430        440        450        460        470        480 
RTIPLTFKDQ FIIAYGGLRG AICFALVFLL PATVFPRKKL FITAAIVVIF FTVFILGITI 

       490        500        510        520        530        540 
RPLVEFLDVK RSNKKQQAVS EEIHCRFFDH VKTGIEDVCG HWGHNFWRDK FKKFDDKYLR 

       550        560        570        580        590        600 
KLLIRENQPK SSIVSLYKKL EIKHAIEMAE TGMISTVPSF ASLNDCREEK IRKLTPGEMD 

       610        620        630        640        650        660 
EIREILSRNL YQIRQRTLSY NRHNLTADTS ERQAKEILIR RRHSLRESLR KDNSLNRERR 

       670        680        690        700        710        720 
ASTSTSRYLS LPKNTKLPEK LQKKNKVSNA DGNSSDSDMD GTTVLNLQPR ARRFLPDQFS 

       730        740        750        760        770        780 
KKASPAYKME WKNEVDVGSA RAPPSVTPAP RSKEGGTQTP GVLRQPLLSK DQRFGRGRED 

       790        800        810 
SLTEDVPPKP PPRLVRRASE PGNRKGRLGN EKP 

« Hide

Isoform Short [UniParc].

Checksum: DD5C1A503CAE9ACE
Show »

FASTA69779,063

References

[1]"Primary structure and functional expression of a novel gastrointestinal isoform of the rat Na/H exchanger."
Wang Z., Orlowski J., Shull G.E.
J. Biol. Chem. 268:11925-11928(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Stomach.
[2]"Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2)."
Collins J.F., Honda T., Knobel S., Bulus N.M., Conary J., Dubois R., Ghishan F.K.
Proc. Natl. Acad. Sci. U.S.A. 90:3938-3942(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Strain: Sprague-Dawley.
Tissue: Small intestine.
[3]"Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+ exchanger (SLC9A2)."
Ghishan F.K., Knobel S.M., Summar M.
Genomics 30:25-30(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Liver.
[4]"Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium."
Inoue H., Nakamura Y., Nagita M., Takai T., Masuda M., Nakamura N., Kanazawa H.
Biol. Pharm. Bull. 26:148-155(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHP1 AND CHP2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11236 mRNA. Translation: AAA72350.1.
L11004 mRNA. Translation: AAA75406.1.
S81591 mRNA. Translation: AAB36180.1.
PIRA46748.
A57644.
RefSeqNP_001106806.1. NM_001113335.1.
NP_036785.2. NM_012653.2.
UniGeneRn.11047.

3D structure databases

ProteinModelPortalP48763.
SMRP48763. Positions 137-162, 231-256, 319-346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP48763. 2 interactions.
STRING10116.ENSRNOP00000021270.

Proteomic databases

PaxDbP48763.
PRIDEP48763.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000021270; ENSRNOP00000021270; ENSRNOG00000015567. [P48763-1]
ENSRNOT00000066000; ENSRNOP00000059336; ENSRNOG00000015567. [P48763-2]
GeneID24783.
KEGGrno:24783.
UCSCRGD:3719. rat. [P48763-1]

Organism-specific databases

CTD6549.
RGD3719. Slc9a2.

Phylogenomic databases

eggNOGCOG0025.
GeneTreeENSGT00560000077158.
HOGENOMHOG000247044.
HOVERGENHBG052615.
InParanoidP48763.
KOK14722.
OMAPQSYKME.
OrthoDBEOG7PCJGB.
PhylomeDBP48763.
TreeFamTF317212.

Gene expression databases

GenevestigatorP48763.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

NextBio604396.
PROP48763.

Entry information

Entry nameSL9A2_RAT
AccessionPrimary (citable) accession number: P48763
Secondary accession number(s): Q16434
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families