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Protein

Carbonyl reductase [NADPH] 1

Gene

Cbr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (By similarity).By similarity

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.
(5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH.
(13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost-13-enoate + NADP+ = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901NADP; via carbonyl oxygenBy similarity
Binding sitei106 – 1061GlutathioneBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3425NADPBy similarityAdd
BLAST
Nucleotide bindingi63 – 642NADPBy similarity
Nucleotide bindingi194 – 1985NADPBy similarity
Nucleotide bindingi231 – 2333NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKP48758.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonyl reductase [NADPH] 1 (EC:1.1.1.184)
Alternative name(s):
15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197)
NADPH-dependent carbonyl reductase 1
Prostaglandin 9-ketoreductase
Prostaglandin-E(2) 9-reductase (EC:1.1.1.189)
Gene namesi
Name:Cbr1
Synonyms:Cbr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:88284. Cbr1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 277276Carbonyl reductase [NADPH] 1PRO_0000054603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei30 – 301PhosphoserineCombined sources
Modified residuei162 – 1621PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP48758.
MaxQBiP48758.
PaxDbiP48758.
PRIDEiP48758.

2D gel databases

REPRODUCTION-2DPAGEP48758.

PTM databases

iPTMnetiP48758.
PhosphoSiteiP48758.
SwissPalmiP48758.

Expressioni

Gene expression databases

BgeeiENSMUSG00000051483.
CleanExiMM_CBR1.
ExpressionAtlasiP48758. baseline and differential.
GenevisibleiP48758. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi198531. 2 interactions.
IntActiP48758. 4 interactions.
MINTiMINT-1869255.
STRINGi10090.ENSMUSP00000049394.

Structurei

3D structure databases

ProteinModelPortaliP48758.
SMRiP48758. Positions 7-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 973Glutathione bindingBy similarity
Regioni193 – 1942Glutathione bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1208. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00510000046499.
HOVERGENiHBG001909.
InParanoidiP48758.
KOiK00079.
OMAiMETFVED.
OrthoDBiEOG091G0V7L.
PhylomeDBiP48758.
TreeFamiTF329359.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSRPVALV TGANKGIGFA ITRDLCRKFS GDVVLAARDE ERGQTAVQKL
60 70 80 90 100
QAEGLSPRFH QLDIDNPQSI RALRDFLLKE YGGLDVLVNN AGIAFKVNDD
110 120 130 140 150
TPFHIQAEVT MKTNFFGTRD VCKELLPLIK PQGRVVNVSS MVSLRALKNC
160 170 180 190 200
RLELQQKFRS ETITEEELVG LMNKFVEDTK KGVHAEEGWP NSAYGVTKIG
210 220 230 240 250
VTVLSRILAR KLNEQRRGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET
260 270
PVYLALLPPD AEGPHGQFVQ DKKVEPW
Length:277
Mass (Da):30,641
Last modified:April 17, 2007 - v3
Checksum:i6053423B6D5F82B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901N → K in AAB19006 (PubMed:8661038).Curated
Sequence conflicti112 – 1121K → E in AAB19006 (PubMed:8661038).Curated
Sequence conflicti218 – 2181G → E in AAB19006 (PubMed:8661038).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31966 mRNA. Translation: AAB19006.1.
BC012714 mRNA. Translation: AAH12714.1.
CCDSiCCDS28341.1.
RefSeqiNP_031646.2. NM_007620.2.
UniGeneiMm.26940.

Genome annotation databases

EnsembliENSMUST00000039659; ENSMUSP00000049394; ENSMUSG00000051483.
GeneIDi12408.
KEGGimmu:12408.
UCSCiuc007zzr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31966 mRNA. Translation: AAB19006.1.
BC012714 mRNA. Translation: AAH12714.1.
CCDSiCCDS28341.1.
RefSeqiNP_031646.2. NM_007620.2.
UniGeneiMm.26940.

3D structure databases

ProteinModelPortaliP48758.
SMRiP48758. Positions 7-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198531. 2 interactions.
IntActiP48758. 4 interactions.
MINTiMINT-1869255.
STRINGi10090.ENSMUSP00000049394.

PTM databases

iPTMnetiP48758.
PhosphoSiteiP48758.
SwissPalmiP48758.

2D gel databases

REPRODUCTION-2DPAGEP48758.

Proteomic databases

EPDiP48758.
MaxQBiP48758.
PaxDbiP48758.
PRIDEiP48758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039659; ENSMUSP00000049394; ENSMUSG00000051483.
GeneIDi12408.
KEGGimmu:12408.
UCSCiuc007zzr.1. mouse.

Organism-specific databases

CTDi873.
MGIiMGI:88284. Cbr1.

Phylogenomic databases

eggNOGiKOG1208. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00510000046499.
HOVERGENiHBG001909.
InParanoidiP48758.
KOiK00079.
OMAiMETFVED.
OrthoDBiEOG091G0V7L.
PhylomeDBiP48758.
TreeFamiTF329359.

Enzyme and pathway databases

ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKP48758.

Miscellaneous databases

PROiP48758.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051483.
CleanExiMM_CBR1.
ExpressionAtlasiP48758. baseline and differential.
GenevisibleiP48758. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBR1_MOUSE
AccessioniPrimary (citable) accession number: P48758
Secondary accession number(s): Q91X28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.