P48757 (GAST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin

Cleaved into the following 3 chains:

1. Gastrin-71
   Short name=G71
2. Big gastrin
   Alternative name(s):
   Gastrin-34
   Short name=G34
3. Gastrin

Gene names

Name:	Gast
Synonyms:	Gas

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Subcellular location	Secreted.
Tissue specificity	Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas. Ref.3
Post-translational modification	Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins and between tissues By similarity.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Phosphoprotein Pyrrolidone carboxylic acid Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Peptide	22 – 92	71	Gastrin-71		PRO_0000010640
	Peptide	59 – 92	34	Big gastrin		PRO_0000010641
	Peptide	76 – 92	17	Gastrin		PRO_0000010642
	Propeptide	96 – 101	6	By similarity		PRO_0000010643
Amino acid modifications
	Modified residue	59	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	76	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	87	1	Sulfotyrosine By similarity
	Modified residue	92	1	Phenylalanine amide
	Modified residue	96	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	45	1	R → G in AAB06947. Ref.2
	Sequence conflict	62	1	P → L in AAB97872. Ref.1
	Sequence conflict	62	1	P → L in AAB06947. Ref.2
	Sequence conflict	62	1	P → L in CAA64385. Ref.3
	Sequence conflict	62	1	P → L in CAA64386. Ref.3
	Sequence conflict	76	1	Q → E in AAB97872. Ref.1
	Sequence conflict	76	1	Q → E in AAB06947. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48757 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 41DE15814DBFB68E	FASTA	101	11,590
	10 20 30 40 50 60 MPRLCVYMLV LVLALATFSE ASWKPRSQLQ DASSGPGTNE DLEQRQFNKL GSASHHRRQL 70 80 90 100 GPQGPQHFIA DLSKKQRPRM EEEEEAYGWM DFGRRSAEED Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of the murine gastrin gene." Koh T.J., Wang T.C. Biochem. Biophys. Res. Commun. 216:34-41(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Sequences responsible for transcription termination of the mouse gastrin gene." Noh M.J., Kim S.J., Kang Y.K., Yoo O.J. Biochem. Mol. Biol. Int. 35:1205-1213(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[3]	"Molecular structure and genetic mapping of the mouse gastrin gene." Friis-Hansen L., Rourke I.J., Bundgaard J.R., Rehfeld J.F., Samuelson L.C. FEBS Lett. 386:128-132(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: 129/SvJ. Tissue: Stomach.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Small intestine and Stomach.
[5]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34293 Genomic DNA. Translation: AAB97872.1. U58136 Genomic DNA. Translation: AAB06947.1. X94760 Genomic DNA. Translation: CAA64386.1. X94758 mRNA. Translation: CAA64385.1. AK008062 mRNA. Translation: BAB25437.1. AK008159 mRNA. Translation: BAB25501.1. AK008313 mRNA. Translation: BAB25596.1. AK008420 mRNA. Translation: BAB25658.1. AK008494 mRNA. Translation: BAB25699.1. AK008649 mRNA. Translation: BAB25807.1. AL590968 Genomic DNA. Translation: CAM23027.1.
IPI	IPI00319752. IPI00556767.
PIR	S68861.
RefSeq	NP_034387.3. NM_010257.3.
UniGene	Mm.4767.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P48757.
Proteomic databases
PRIDE	P48757.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000017309; ENSMUSP00000017309; ENSMUSG00000017165.
GeneID	14459.
KEGG	mmu:14459.
Organism-specific databases
CTD	2520.
MGI	MGI:104768. Gast.
Phylogenomic databases
eggNOG	NOG39909.
GeneTree	ENSGT00390000014792.
HOGENOM	HOG000073533.
HOVERGEN	HBG097593.
InParanoid	P48757.
KO	K13768.
OMA	KPRSQLQ.
OrthoDB	EOG4CG09S.
Gene expression databases
CleanEx	MM_GAST.
Genevestigator	P48757.
GermOnline	ENSMUSG00000017165. Mus musculus.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286088.
SOURCE	Search...

Entry information

Entry name

GAST_MOUSE

Accession

Primary (citable) accession number: P48757
Secondary accession number(s): P70334, Q64295, Q9CPR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program