P48750 (CSF2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granulocyte-macrophage colony-stimulating factor
Short name=GM-CSF

Alternative name(s):
Colony-stimulating factor
Short name=CSF

Gene names

Name:	Csf2
Synonyms:	Csfgm

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes By similarity.
Subunit structure	Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the GM-CSF family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to lipopolysaccharide Inferred from expression pattern. Source: RGD dendritic cell differentiation Inferred from direct assay. Source: RGD epithelial cell proliferation Traceable author statement. Source: RGD epithelial fluid transport Inferred from direct assay. Source: RGD immune response Inferred from electronic annotation. Source: InterPro macrophage activation Inferred from mutant phenotype. Source: RGD positive regulation of macrophage differentiation Traceable author statement. Source: RGD
Cellular component	extracellular space Inferred from direct assay. Source: RGD
Molecular function	cytokine activity Inferred from direct assay. Source: RGD granulocyte macrophage colony-stimulating factor receptor binding Inferred from electronic annotation. Source: InterPro growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 144	127	Granulocyte-macrophage colony-stimulating factor		PRO_0000144833
Amino acid modifications
	Glycosylation	22	1	O-linked (GalNAc...) By similarity
	Glycosylation	27	1	O-linked (GalNAc...) By similarity
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Disulfide bond	71 ↔ 113		By similarity
	Disulfide bond	105 ↔ 138		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48750 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 7385E93CC40836B1	FASTA	144	16,562
	10 20 30 40 50 60 MWLQNLLFLG IVVYSFSAPT RSPNPVTRPW KHVDAIKEAL SLLNDMRALE NEKNEDVDII 70 80 90 100 110 120 SNEFSIQRPT CVQTRLKLYK QGLRGNLTKL NGALTMIASH YQTNCPPTPE TDCEIEVTTF 130 140 EDFIKNLKGF LFDIPFDCWK PVQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"Nucleotide sequence of the Lewis rat granulocyte-macrophage colony stimulating factor." Smith L.R., Lundeen K.A., Diveley J.P., Carlo D.J., Brostoff S.W. Immunogenetics 39:80-80(1994) [PubMed: 8225444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-144. Strain: Lewis. Tissue: Splenocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABR03075230 Genomic DNA. No translation available. U00620 mRNA. Translation: AAA18281.1.
IPI	IPI00372574.
PIR	I46269.
RefSeq	XP_001074265.1. XM_001074265.1. XP_340800.3. XM_340799.3.
UniGene	Rn.44285.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P48750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000032333; ENSRNOP00000034235; ENSRNOG00000026805.
GeneID	116630.
KEGG	rno:116630.
Organism-specific databases
CTD	1437.
RGD	621065. Csf2.
Phylogenomic databases
eggNOG	roNOG17708.
GeneTree	ENSGT00390000013425.
HOVERGEN	HBG000532.
InParanoid	P48750.
OMA	TCLQTRL.
OrthoDB	EOG4255V3.
Gene expression databases
ArrayExpress	P48750.
Genevestigator	P48750.
GermOnline	ENSRNOG00000026805. Rattus norvegicus.
Family and domain databases
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR000773. GM_colony-stim-fac. [Graphical view]
Gene3D	G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.
KO	K05427.
PANTHER	PTHR10059. GM_CSF. 1 hit.
Pfam	PF01109. GM_CSF. 1 hit. [Graphical view]
PRINTS	PR00693. GMCSFACTOR.
ProDom	PD007349. GM_colony-stim-fac. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00040. CSF2. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00702. GM_CSF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSF2_RAT

Accession

Primary (citable) accession number: P48750

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 25, 2008
Last modified:	November 16, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program