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Protein

Complement component C9

Gene

C9

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.By similarity

GO - Biological processi

Keywordsi

Biological processComplement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C9
Gene namesi
Name:C9
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei319 – 335Beta strandedSequence analysisAdd BLAST17
Transmembranei340 – 359Beta strandedSequence analysisAdd BLAST20

Keywords - Cellular componenti

Membrane, Membrane attack complex, Secreted, Target cell membrane, Target membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000002360622 – 557Complement component C9Add BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 78By similarity
Disulfide bondi54 ↔ 57By similarity
Disulfide bondi88 ↔ 94By similarity
Disulfide bondi101 ↔ 113By similarity
Disulfide bondi108 ↔ 126By similarity
Disulfide bondi120 ↔ 135By similarity
Disulfide bondi143 ↔ 182By similarity
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi385 ↔ 414By similarity
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi520 ↔ 536By similarity
Disulfide bondi523 ↔ 538By similarity
Disulfide bondi540 ↔ 549By similarity

Post-translational modificationi

Thrombin cleaves factor C9 to produce C9a and C9b.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. About 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes.By similarity

Protein-protein interaction databases

CORUMiP48747.
STRINGi9986.ENSOCUP00000010939.

Structurei

3D structure databases

ProteinModelPortaliP48747.
SMRiP48747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 95TSP type-1Add BLAST54
Domaini99 – 137LDL-receptor class AAdd BLAST39
Domaini139 – 519MACPFAdd BLAST381
Domaini520 – 550EGF-likeAdd BLAST31

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG410IH3C. Eukaryota.
ENOG410XT9A. LUCA.
HOGENOMiHOG000111869.
HOVERGENiHBG106792.
InParanoidiP48747.
KOiK04000.

Family and domain databases

CDDicd00112. LDLa. 1 hit.
InterProiView protein in InterPro
IPR013032. EGF-like_CS.
IPR036055. LDL_receptor-like_sf.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
IPR036383. TSP1_rpt_sf.
PfamiView protein in Pfam
PF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 1 hit.
PRINTSiPR00764. COMPLEMENTC9.
SMARTiView protein in SMART
SM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 1 hit.
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiView protein in PROSITE
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48747-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASHSFAFV VCVLEIGALT AGPTPSYVHE PIQRSDPLQP IDCRMSPWSE
60 70 80 90 100
WSHCDPCLRQ MFRSRSIEVF GQFHGKSCVD ALGDRRACIP TEACEDAEED
110 120 130 140 150
CEKDEFHCGT GRCIKRRLLC NGDNDCGDFS DEDDCETEPR LTCRNREVQE
160 170 180 190 200
SELARTAGYG INILGMDPLA TPFDNEYYHG LCDRVWDGNT LTHYRKPWNV
210 220 230 240 250
AVLAYETKID KNFRTEYYEE QMQAFKSIIE EETSNFNANL ALKFTPTEAK
260 270 280 290 300
ASKAEEASPK NKSLDDNDKG FSSKFQFSYS KNETYQLFLS YSSQKEKMFL
310 320 330 340 350
LVKGIIQLGR FVMKNRGVML TNTFLDDIKS LPTTYEKGEY FAFLETYGTH
360 370 380 390 400
YSSSGSLGGR YELIYVLDKA SMKEKGIELN DIKKCLGFDL DLSLNIPGKS
410 420 430 440 450
AGLSLTGQAN KNNCLKSGHG NAVNITRANL IDDVISLIRG GTQKFAFELK
460 470 480 490 500
EKLLTKAKMV DVTDFINWAS SLSDAPVLIN QKLSPIYNLI PVKIKDAHQK
510 520 530 540 550
RQNLERGIED YINEFSTKKC SPCQNGGTAL LMDGQCLCTC PFMFEGIACE

ISKRKLA
Length:557
Mass (Da):62,662
Last modified:February 1, 1996 - v1
Checksum:iFF4E65FF8D1AB417
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20055 mRNA. Translation: AAC48459.1.
RefSeqiNP_001075815.1. NM_001082346.1.
UniGeneiOcu.2269.

Genome annotation databases

GeneIDi100009197.
KEGGiocu:100009197.

Similar proteinsi

Entry informationi

Entry nameiCO9_RABIT
AccessioniPrimary (citable) accession number: P48747
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families