P48745 (NOV_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NOV homolog
Short name=NovH

Alternative name(s):
CCN family member 3
Insulin-like growth factor-binding protein 9
Short name=IBP-9
Short name=IGF-binding protein 9
Short name=IGFBP-9
Nephroblastoma-overexpressed gene protein homolog

Gene names

Name:	NOV
Synonyms:	CCN3, IGFBP9, NOVH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Immediate-early protein likely to play a role in cell growth regulation By similarity.
Subunit structure	Interacts with FBLN1. Ref.9
Subcellular location	Secreted.
Tissue specificity	Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. Ref.7
Induction	Expression is down-regulated by WT1. Ref.2
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell growth Inferred from electronic annotation. Source: InterPro regulation of gene expression Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara extracellular matrix Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA neuronal cell body Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.8
	Chain	32 – 357	326	Protein NOV homolog		PRO_0000014415
Regions
	Domain	32 – 105	74	IGFBP N-terminal
	Domain	108 – 174	67	VWFC
	Domain	205 – 250	46	TSP type-1
	Domain	264 – 338	75	CTCK
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Disulfide bond	264 ↔ 301		By similarity
	Disulfide bond	281 ↔ 315		By similarity
	Disulfide bond	292 ↔ 331		By similarity
	Disulfide bond	295 ↔ 333		By similarity
	Disulfide bond	300 ↔ 337		By similarity
Natural variations
	Natural variant	42	1	R → Q. Corresponds to variant rs2279112 [ dbSNP | Ensembl ].		VAR_049568
	Natural variant	233	1	R → H. Corresponds to variant rs11538929 [ dbSNP | Ensembl ].		VAR_049569
Experimental info
	Sequence conflict	26 – 27	2	LG → CL Ref.7
	Sequence conflict	97	1	N → K in CAG33713. Ref.5
	Sequence conflict	97	1	N → K in AAH15028. Ref.6
	Sequence conflict	231	1	R → G in BAG35274. Ref.4
	Sequence conflict	357	1	M → I in CAG33713. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P48745 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 035D5BF4576BD85B	FASTA	357	39,162
	10 20 30 40 50 60 MQSVQSTSFC LRKQCLCLTF LLLHLLGQVA ATQRCPPQCP GRCPATPPTC APGVRAVLDG 70 80 90 100 110 120 CSCCLVCARQ RGESCSDLEP CDESSGLYCD RSADPSNQTG ICTAVEGDNC VFDGVIYRSG 130 140 150 160 170 180 EKFQPSCKFQ CTCRDGQIGC VPRCQLDVLL PEPNCPAPRK VEVPGECCEK WICGPDEEDS 190 200 210 220 230 240 LGGLTLAAYR PEATLGVEVS DSSVNCIEQT TEWTACSKSC GMGFSTRVTN RNRQCEMLKQ 250 260 270 280 290 300 TRLCMVRPCE QEPEQPTDKK GKKCLRTKKS LKAIHLQFKN CTSLHTYKPR FCGVCSDGRC 310 320 330 340 350 CTPHNTKTIQ AEFQCSPGQI VKKPVMVIGT CTCHTNCPKN NEAFLQELEL KTTRGKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the human nov proto-oncogene and expression in Wilms tumor." Martinerie C., Huff V., Joubert I., Badzioch M., Saunders G.F., Strong L.C., Perbal B. Oncogene 9:2729-2732(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[2]	"Regulation of nov by WT1: a potential role for nov in nephrogenesis." Martinerie C., Chevalier G., Rauscher F.J. III, Perbal B. Oncogene 12:1479-1492(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[3]	"Cloning, sequencing and expression of human nov gene." Jiang D., Gou D., Li W. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[7]	"Expression of a gene encoding a novel potential IGF binding protein in human tissues." Martinerie C., Perbal B. C. R. Acad. Sci. III, Sci. Vie 313:345-351(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-102, TISSUE SPECIFICITY.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-46.
[9]	"The C-terminal domain of the regulatory protein NOVH is sufficient to promote interaction with fibulin 1C: a clue for a role of NOVH in cell-adhesion signaling." Perbal B., Martinerie C., Sainson R., Werner M., He B., Roizman B. Proc. Natl. Acad. Sci. U.S.A. 96:869-874(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FBLN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78351 , X78352, X78353, X78354 Genomic DNA. Translation: CAA55146.1. X96584 mRNA. Translation: CAA65403.1. AY082381 mRNA. Translation: AAL92490.1. AK312355 mRNA. Translation: BAG35274.1. CR457432 mRNA. Translation: CAG33713.1. BC015028 mRNA. Translation: AAH15028.1.
IPI	IPI00011140.
PIR	I38069.
RefSeq	NP_002505.1. NM_002514.3.
UniGene	Hs.235935.
3D structure databases
ProteinModelPortal	P48745.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48745. 1 interaction.
STRING	9606.ENSP00000259526.
Polymorphism databases
DMDM	1352515.
Proteomic databases
PaxDb	P48745.
PeptideAtlas	P48745.
PRIDE	P48745.
Protocols and materials databases
DNASU	4856.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259526; ENSP00000259526; ENSG00000136999.
GeneID	4856.
KEGG	hsa:4856.
UCSC	uc003yoq.2. human.
Organism-specific databases
CTD	4856.
GeneCards	GC08P120428.
HGNC	HGNC:7885. NOV.
HPA	HPA018449. HPA019684.
MIM	164958. gene.
neXtProt	NX_P48745.
PharmGKB	PA31687.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG72934.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	P48745.
OMA	CCTPHNT.
OrthoDB	EOG4QZ7M8.
PhylomeDB	P48745.
Gene expression databases
ArrayExpress	P48745.
Bgee	P48745.
CleanEx	HS_NOV.
Genevestigator	P48745.
GermOnline	ENSG00000136999. Homo sapiens.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00047. Insulin Glargine recombinant. DB00046. Insulin Lyspro recombinant. DB00030. Insulin recombinant. DB00071. Insulin, porcine.
GenomeRNAi	4856.
NextBio	18706.
SOURCE	Search...

Entry information

Entry name

NOV_HUMAN

Accession

Primary (citable) accession number: P48745
Secondary accession number(s): B2R5X7 , Q6I9S3, Q96BY5, Q9UDE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.