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Reviewed, UniProtKB/Swiss-Prot P48736 (PK3CG_HUMAN)

Last modified July 7, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
    EC=2.7.1.153
Alternative name(s):
    PI3-kinase p110 subunit gamma
    PtdIns-3-kinase subunit p110
    PI3Kgamma
      Short name=PI3K
    p120-PI3K
Gene names
Name: PIK3CG
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length1102 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.

Enzyme regulation

Activated by both the alpha and the beta-gamma G proteins. Ref.1

Pathway

Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

Subunit structure

Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit.

Tissue specificity

Pancreas, skeletal muscle, liver and heart. Ref.1

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 PI3K/PI4K domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

HRASP011121EBI-1030384,EBI-350145

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11021102Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
PRO_0000088792

Regions

Domain828 – 1073246PI3K/PI4K
Compositional bias19 – 235Poly-Arg

Experimental info

Sequence conflict301Missing in CAA58284. Ref.1
Sequence conflict4591Q → R Ref.1
Sequence conflict4591Q → R Ref.3

Secondary structure

1 .................................................................................................................................... 1102
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P48736-1 [UniParc].

Last modified April 4, 2006. Version 3.
Checksum: EF2B1A0E1CBEF406

FASTA1,102126,454
        10         20         30         40         50         60 
MELENYKQPV VLREDNCRRR RRMKPRSAAA SLSSMELIPI EFVLPTSQRK CKSPETALLH 

        70         80         90        100        110        120 
VAGHGNVEQM KAQVWLRALE TSVAADFYHR LGPHHFLLLY QKKGQWYEIY DKYQVVQTLD 

       130        140        150        160        170        180 
CLRYWKATHR SPGQIHLVQR HPPSEESQAF QRQLTALIGY DVTDVSNVHD DELEFTRRGL 

       190        200        210        220        230        240 
VTPRMAEVAS RDPKLYAMHP WVTSKPLPEY LWKKIANNCI FIVIHRSTTS QTIKVSPDDT 

       250        260        270        280        290        300 
PGAILQSFFT KMAKKKSLMD IPESQSEQDF VLRVCGRDEY LVGETPIKNF QWVRHCLKNG 

       310        320        330        340        350        360 
EEIHVVLDTP PDPALDEVRK EEWPLVDDCT GVTGYHEQLT IHGKDHESVF TVSLWDCDRK 

       370        380        390        400        410        420 
FRVKIRGIDI PVLPRNTDLT VFVEANIQHG QQVLCQRRTS PKPFTEEVLW NVWLEFSIKI 

       430        440        450        460        470        480 
KDLPKGALLN LQIYCGKAPA LSSKASAESP SSESKGKVQL LYYVNLLLID HRFLLRRGEY 

       490        500        510        520        530        540 
VLHMWQISGK GEDQGSFNAD KLTSATNPDK ENSMSISILL DNYCHPIALP KHQPTPDPEG 

       550        560        570        580        590        600 
DRVRAEMPNQ LRKQLEAIIA TDPLNPLTAE DKELLWHFRY ESLKHPKAYP KLFSSVKWGQ 

       610        620        630        640        650        660 
QEIVAKTYQL LARREVWDQS ALDVGLTMQL LDCNFSDENV RAIAVQKLES LEDDDVLHYL 

       670        680        690        700        710        720 
LQLVQAVKFE PYHDSALARF LLKRGLRNKR IGHFLFWFLR SEIAQSRHYQ QRFAVILEAY 

       730        740        750        760        770        780 
LRGCGTAMLH DFTQQVQVIE MLQKVTLDIK SLSAEKYDVS SQVISQLKQK LENLQNSQLP 

       790        800        810        820        830        840 
ESFRVPYDPG LKAGALAIEK CKVMASKKKP LWLEFKCADP TALSNETIGI IFKHGDDLRQ 

       850        860        870        880        890        900 
DMLILQILRI MESIWETESL DLCLLPYGCI STGDKIGMIE IVKDATTIAK IQQSTVGNTG 

       910        920        930        940        950        960 
AFKDEVLNHW LKEKSPTEEK FQAAVERFVY SCAGYCVATF VLGIGDRHND NIMITETGNL 

       970        980        990       1000       1010       1020 
FHIDFGHILG NYKSFLGINK ERVPFVLTPD FLFVMGTSGK KTSPHFQKFQ DICVKAYLAL 

      1030       1040       1050       1060       1070       1080 
RHHTNLLIIL FSMMLMTGMP QLTSKEDIEY IRDALTVGKN EEDAKKYFLD QIEVCRDKGW 

      1090       1100 
TVQFNWFLHL VLGIKQGEKH SA

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase."
Stoyanov B., Volinia S., Hanck T., Rubio I., Loubtchenkov M., Malek D., Stoyanova S., Vanhaesebroeck B., Dhand R., Nuernberg B., Gierschik P., Seedorf K., Hsuan J.J., Waterfield M.D., Wetzker R.
Science 269:690-693(1995) [PubMed: 7624799] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.
[2]Waterfield M.D.
Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Regulation of a G-protein-activated phosphoinositide-3-kinase."
Michalke M., Schaefer M., Stoyanov B., Wetzker R., Nuernberg B.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[6]"p84, a new Gbetagamma-activated regulatory subunit of the type IB phosphoinositide 3-kinase p110gamma."
Suire S., Coadwell J., Ferguson G.J., Davidson K., Hawkins P., Stephens L.
Curr. Biol. 15:566-570(2005) [PubMed: 15797027] [Abstract]
Cited for: INTERACTION WITH PIK3R5.
[7]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]"Structural insights into phosphoinositide 3-kinase catalysis and signalling."
Walker E.H., Perisic O., Ried C., Stephens L., Williams R.L.
Nature 402:313-320(1999) [PubMed: 10580505] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 144-1102.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X83368 mRNA. Translation: CAA58284.1.
AF327656 mRNA. Translation: AAG61115.1.
AC005018 Genomic DNA. Translation: AAQ96873.1.
BC035683 mRNA. Translation: AAH35683.1.
IPIIPI00292690.
RefSeqNP_002640.2.
UniGeneHs.32942

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1E8YX-ray2.00A144-1102[»]
1E8ZX-ray2.40A144-1102[»]
1HE8X-ray3.00A144-1102[»]
2A4ZX-ray2.90A144-1101[»]
2A5UX-ray2.70A144-1101[»]
2CHWX-ray2.60A144-1101[»]
2CHXX-ray2.50A144-1101[»]
2CHZX-ray2.60A144-1101[»]
2V4LX-ray2.50A144-1102[»]
3CSFX-ray2.80A144-1102[»]
3CSTX-ray3.20A144-1102[»]
3DBSX-ray2.80A144-1102[»]
3DPDX-ray2.85A144-1102[»]
3ENEX-ray2.40A144-1102[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP48736. 1 interaction.

PTM databases

PhosphoSiteP48736.

Proteomic databases

PRIDEP48736.

Genome annotation databases

EnsemblENSG00000105851. Homo sapiens. [Contig view]
GeneID5294.
KEGGhsa:5294.
UCSCuc003vdu.1. human.

Organism-specific databases

GeneCardsGC07P106292.
H-InvDBHIX0025282.
HGNCHGNC:8978. PIK3CG.
HPACAB010283.
MIM601232. gene.
PharmGKBPA33311.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP48736.
HOVERGENP48736.
OMAP48736. WYEIYDK.

Enzyme and pathway databases

BRENDA2.7.1.137. 247.
2.7.1.153. 247.
Pathway_Interaction_DBpi3kcipathway. Class I PI3K signaling events.
pi3kcibpathway. Class IB PI3K non-lipid kinase events.
epha_fwdpathway. EPHA forward signaling.
txa2pathway. Thromboxane A2 receptor signaling.

Gene expression databases

ArrayExpressP48736.
BgeeP48736.
CleanExHS_PIK3CG.
GermOnlineENSG00000105851. Homo sapiens.

Family and domain databases

InterProIPR000403. PI3/4_kinase_cat.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2.
IPR000341. PI3K_ras_bd.
IPR001263. PI3Ka.
IPR015433. PI_Kinase.
[Graphical view]
Gene3DG3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHERPTHR10048. PI_Kinase. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio20458.
SOURCESearch...

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Entry information

Entry namePK3CG_HUMAN
AccessionPrimary (citable) accession number: P48736
Secondary accession number(s): Q8IV23, Q9BZC8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 4, 2006
Last modified: July 7, 2009
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents